Main Article Content
Abstract
[EXPLORATION AND PATHOGENICITY TEST OF Beauveria bassiana AGAINST ARMYWORMS (Spodoptera litura F.)]. Armyworm (Spodoptera litura) is a pest that can attack a variety of host plants. S. litura has a chewing bite mouth type and the phase that causes the most damage is instar 2, if no control is done then S. litura can cause damage up to 80% or cause crop failure. Control of S. litura still relies on chemical pesticides, this can have negative effects in the long term so alternative control is needed. Beauveria bassiana is an entomopathogenic fungus found in soil and has been studied to be able to control various types of insects at almost all stages of insects, including larvae and imago. The purpose of this study was to determine the effectiveness of B. bassiana fungus in controlling S. litura. The method used was the spore density level of 106, 107, 108 conidia/mL and the type of isolate B1 (isolate origin from Pacet) and B2 (isolate origin from Trawas), while the observation parameters were mortality rate and feeding rate. The results obtained were the treatment of isolate B1 at a spore density of 108 conidia/mL was able to cause the highest mortality of 80% while the rate of feeding power of isolate B1 treatment with a spore density of 108 conidia/mL was able to reduce the appetite of S. litura most effectively.
Article Details
Copyright (c) 2024 Jurnal Ilmu-Ilmu Pertanian Indonesia
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
References
- Alkapi, Sukra. (2021). Uji efektivitas jamur entomopatogen terhadap larva penggerek batang kelapa sawit (Oryctes rhinoceros I.) di laboratorium. Jurnal Riset Perkebunan, 2(2), 69–75. DOI: https://doi.org/10.25077/jrp.2.2.69-75. 2021.
- Aufa, N. & Jadmiko, W. (2023). Penambahan beberapa jenis tepung serangga pada media perbanyakan jamur Metarhizium anisopliae (Metsch.) sorokin guna meningkatkan virulensinya terhadap hama Crocidolomia pavonana Fabricius di laboratorium. Berkala Ilmiah Pertanian, 6(4), 215. DOI: https://doi. org/10.19184/bip.v6i4.39288.
- Budi, A. S., Afandhi, A. & Dyah Puspitarini, R. (2013). Patogenisitas jamur entomopatogen Beauveria bassiana Balsamo (Deuteromycetes: Moniliales) pada larva Spodoptera litura Fabricius (Lepidoptera: Noctuidae). Jurnal HPT (Hama Penyakit Tumbuhan), 1(1), 57–65.
- Daud, I. D., Elkawakib, Mustari, K., Baso, A. & Widiayani, N. (2020). Infection of Ostrinia furnacalis (Lepidoptera: Pyralidae) by endophytic Beauveria bassiana on corn. Online Journal of Biological Sciences, 20(1), 1–7. DOI: https://doi.org/10.3844/ojbsci.2020.1.7 .
- Fattah, A. & Ilyas, A. (2016). Siklus hidup ulat grayak (Spodoptera litura F.) dan tingkat serangan pada beberapa varietas unggul kedelai di Sulawesi Selatan. Prosiding Seminar Nasional Inovasi Teknologi Pertanian, 834–842.
- Hasyim, A., Setiawati, W., Lukman, L. & Marhaeni, L.S. (2019). Evaluasi konsentrasi lethal dan waktu lethal insektisida botani terhadap ulat bawang (Spodoptera exigua) di laboratorium. Jurnal Hortikultura, 29(1), 69–80.
- Kurniawan, R. & Yuniarto, B. (2016). Analisis Regresi : Dasar dan Penerapannya dengan R. Kencana, Jakarta.
- Nyoman Ana Andari, N., Yunus, M. & Asrul. (2020). Pengaruh masa inkubasi biakan Trichoderma sp. terhadap kerapatan spora dan viabilitasnya. Mitra Sains , 8(1), 95–103.
- Pertiwi, S. A. & Haryadi, N.T. (2022). Uji toksisitas jamur Metarhizium anisopliae terhadap hama ulat krop kubis Crocidolomia binotalis Zell. JURNAL AGRI-TEK : Jurnal Penelitian Ilmu-Ilmu Eksakta, 23(2), 15–20. DOI: https://doi.org/10.33319/agtek.v23i2.116
- Prayogo, Y. (2013). Patogenisitas cendawan entomopatogen Beauveria bassiana (Deutero-mycotina: Hyphomycetes) pada berbagai stadia kepik hijau (Nezara viridula l.). Jurnal Hama dan Penyakit Tumbuhan Tropika, 13(1), 75–86. DOI: https://doi.org/10.23960/j.hptt. 11375-86.
- Puji Priyatno, T., Made Samudra, I., Manzila, I., Ningsih Susilowati, D. & Suryadi, Y. (2016). Eksplorasi dan karakterisasi entomopatogen asal berbagai inang dan lokasi. Jurnal Ilmu-Ilmu Hayati , 15(1), 69–79.
- Rosmiati, A., Hidayat, C., Firmansyah, E. & Setiati, Y. (2018). Potensi Beauveria bassiana sebagai agens hayati Spodoptera litura Fabr. pada tanaman kedelai. Agrikultura, 29(1), 43. DOI: https://doi.org/10.24198/agrikultura.v29i1. 16925.
- S, Ahmad., Ilmi, N., Ambar, A. A. & Nuraliyah. (2022). Description of infection symptoms in armyworm larvae (Spodoptera litura F.) by the insect pathogen Beauveria bassiana (Bals.). Seminar Nasional LPPM UMMAT, 1, 346–351.
- Safitri, A., Herlinda, S. & Setiawan, A. (2018). Entomopathogenic fungi of soils of freshwater swamps, tidal lowlands, peatlands, and highlands of South Sumatra, Indonesia. Biodiversitas Journal of Biological Diversity, 19(6), 2365–2373. DOI: https://doi.org/10.13057/biodiv/d190647.
- Saputro, T. B., Yusmani, P., Faradiba, Lazuardi R., Alami, N. H. (2019). The virulence improvement of Beauveria bassiana in infecting Cylas formicarius modulated by various chitin based compounds. Biodiversitas Journal of Biological Diversity, 20(9). DOI: https://doi.org/ 10.13057/biodiv/d200909 .
- Senthil Kumar, C. M., Jacob, T. K., Devasahayam, S., D’Silva, S. & Nandeesh, P. G. (2016). Characterization and virulence of Beauveria bassiana associated with auger beetle (Sinoxylon anale) infesting allspice (Pimenta dioica). Journal of Invertebrate Pathology, 139, 67–73. DOI: https://doi.org/10.1016/j.jip.2016. 07.016.
- Sopialena, S., Sahid, A. & Hutajulu, J. (2022). Uji efektivitas jamur Metarhizium anisoplae dan Beauveria bassiana Bals. lokal dan komerisial terhadap hama kutu daun (Aphis craccivora) pada tanaman kacang panjang (Vigna sinensis L.). Agrifor, 21(1), 147. DOI: https://doi.org/ 10.31293/agrifor.v21i1.5939.
- Suciatmih, Kartika, T. & Yusuf, S. (2015). Jamur entomopatogen dan aktivitas enzim ekstraselulernya. Berita Biologi, 14(2), 131–142.
- Swathi, P., Visalakshy, P. N. G. & Das, S. B. (2017). Potentiality of Beauveria bassiana strains against Helicoverpa armigera through laboratory bioassay. Journal of Entomology and Zoology Studies, 5, 463–467.
- Syamsulhadi, M., Ramadhan, V. T. & Widjayanti, T. (2023). Pertumbuhan jamur Beauveria bassiana pada beberapa tingkat keasaman media dan suhu penyimpanan serta efektivitasnya terhadap hama Spodoptera litura. Jurnal Hama dan Penyakit Tumbuhan, 11(1), 28–41. DOI: https://doi.org/10.21776/ub.jurnalhpt. 2023. 011.1.4.
- Thalib, R., Fernando, R., Khodijah, K., Meidalima, D. & Herlinda, S. (2013). Patogenisitas isolat Beauveria bassiana dan Metarhizium anisopliae asal tanah lebak dan pasang surut sumatera selatan untuk agens hayati Scirpophaga incertulas. Jurnal Hama dan Penyakit Tumbuhan Tropika, 13(1), 10–18. DOI: https://doi.org/10. 23960/j.hptt.11310-18.
- Utami, R. S., Isnawati & Ambarwati, R. (2014). Eksplorasi dan karakterisasi cendawan entomopatogen Beauveria bassiana dari kabupaten malang dan magetan. LenteraBio, 3(1), 59–66.
- Wang, H., Peng, H., Li, W., Cheng, P. & Gong, M. (2021). The toxins of Beauveria bassiana and the strategies to improve their virulence to insects. Frontiers in Microbiology, 12. DOI: https://doi.org/10.3389/fmicb.2021.705343
References
Alkapi, Sukra. (2021). Uji efektivitas jamur entomopatogen terhadap larva penggerek batang kelapa sawit (Oryctes rhinoceros I.) di laboratorium. Jurnal Riset Perkebunan, 2(2), 69–75. DOI: https://doi.org/10.25077/jrp.2.2.69-75. 2021.
Aufa, N. & Jadmiko, W. (2023). Penambahan beberapa jenis tepung serangga pada media perbanyakan jamur Metarhizium anisopliae (Metsch.) sorokin guna meningkatkan virulensinya terhadap hama Crocidolomia pavonana Fabricius di laboratorium. Berkala Ilmiah Pertanian, 6(4), 215. DOI: https://doi. org/10.19184/bip.v6i4.39288.
Budi, A. S., Afandhi, A. & Dyah Puspitarini, R. (2013). Patogenisitas jamur entomopatogen Beauveria bassiana Balsamo (Deuteromycetes: Moniliales) pada larva Spodoptera litura Fabricius (Lepidoptera: Noctuidae). Jurnal HPT (Hama Penyakit Tumbuhan), 1(1), 57–65.
Daud, I. D., Elkawakib, Mustari, K., Baso, A. & Widiayani, N. (2020). Infection of Ostrinia furnacalis (Lepidoptera: Pyralidae) by endophytic Beauveria bassiana on corn. Online Journal of Biological Sciences, 20(1), 1–7. DOI: https://doi.org/10.3844/ojbsci.2020.1.7 .
Fattah, A. & Ilyas, A. (2016). Siklus hidup ulat grayak (Spodoptera litura F.) dan tingkat serangan pada beberapa varietas unggul kedelai di Sulawesi Selatan. Prosiding Seminar Nasional Inovasi Teknologi Pertanian, 834–842.
Hasyim, A., Setiawati, W., Lukman, L. & Marhaeni, L.S. (2019). Evaluasi konsentrasi lethal dan waktu lethal insektisida botani terhadap ulat bawang (Spodoptera exigua) di laboratorium. Jurnal Hortikultura, 29(1), 69–80.
Kurniawan, R. & Yuniarto, B. (2016). Analisis Regresi : Dasar dan Penerapannya dengan R. Kencana, Jakarta.
Nyoman Ana Andari, N., Yunus, M. & Asrul. (2020). Pengaruh masa inkubasi biakan Trichoderma sp. terhadap kerapatan spora dan viabilitasnya. Mitra Sains , 8(1), 95–103.
Pertiwi, S. A. & Haryadi, N.T. (2022). Uji toksisitas jamur Metarhizium anisopliae terhadap hama ulat krop kubis Crocidolomia binotalis Zell. JURNAL AGRI-TEK : Jurnal Penelitian Ilmu-Ilmu Eksakta, 23(2), 15–20. DOI: https://doi.org/10.33319/agtek.v23i2.116
Prayogo, Y. (2013). Patogenisitas cendawan entomopatogen Beauveria bassiana (Deutero-mycotina: Hyphomycetes) pada berbagai stadia kepik hijau (Nezara viridula l.). Jurnal Hama dan Penyakit Tumbuhan Tropika, 13(1), 75–86. DOI: https://doi.org/10.23960/j.hptt. 11375-86.
Puji Priyatno, T., Made Samudra, I., Manzila, I., Ningsih Susilowati, D. & Suryadi, Y. (2016). Eksplorasi dan karakterisasi entomopatogen asal berbagai inang dan lokasi. Jurnal Ilmu-Ilmu Hayati , 15(1), 69–79.
Rosmiati, A., Hidayat, C., Firmansyah, E. & Setiati, Y. (2018). Potensi Beauveria bassiana sebagai agens hayati Spodoptera litura Fabr. pada tanaman kedelai. Agrikultura, 29(1), 43. DOI: https://doi.org/10.24198/agrikultura.v29i1. 16925.
S, Ahmad., Ilmi, N., Ambar, A. A. & Nuraliyah. (2022). Description of infection symptoms in armyworm larvae (Spodoptera litura F.) by the insect pathogen Beauveria bassiana (Bals.). Seminar Nasional LPPM UMMAT, 1, 346–351.
Safitri, A., Herlinda, S. & Setiawan, A. (2018). Entomopathogenic fungi of soils of freshwater swamps, tidal lowlands, peatlands, and highlands of South Sumatra, Indonesia. Biodiversitas Journal of Biological Diversity, 19(6), 2365–2373. DOI: https://doi.org/10.13057/biodiv/d190647.
Saputro, T. B., Yusmani, P., Faradiba, Lazuardi R., Alami, N. H. (2019). The virulence improvement of Beauveria bassiana in infecting Cylas formicarius modulated by various chitin based compounds. Biodiversitas Journal of Biological Diversity, 20(9). DOI: https://doi.org/ 10.13057/biodiv/d200909 .
Senthil Kumar, C. M., Jacob, T. K., Devasahayam, S., D’Silva, S. & Nandeesh, P. G. (2016). Characterization and virulence of Beauveria bassiana associated with auger beetle (Sinoxylon anale) infesting allspice (Pimenta dioica). Journal of Invertebrate Pathology, 139, 67–73. DOI: https://doi.org/10.1016/j.jip.2016. 07.016.
Sopialena, S., Sahid, A. & Hutajulu, J. (2022). Uji efektivitas jamur Metarhizium anisoplae dan Beauveria bassiana Bals. lokal dan komerisial terhadap hama kutu daun (Aphis craccivora) pada tanaman kacang panjang (Vigna sinensis L.). Agrifor, 21(1), 147. DOI: https://doi.org/ 10.31293/agrifor.v21i1.5939.
Suciatmih, Kartika, T. & Yusuf, S. (2015). Jamur entomopatogen dan aktivitas enzim ekstraselulernya. Berita Biologi, 14(2), 131–142.
Swathi, P., Visalakshy, P. N. G. & Das, S. B. (2017). Potentiality of Beauveria bassiana strains against Helicoverpa armigera through laboratory bioassay. Journal of Entomology and Zoology Studies, 5, 463–467.
Syamsulhadi, M., Ramadhan, V. T. & Widjayanti, T. (2023). Pertumbuhan jamur Beauveria bassiana pada beberapa tingkat keasaman media dan suhu penyimpanan serta efektivitasnya terhadap hama Spodoptera litura. Jurnal Hama dan Penyakit Tumbuhan, 11(1), 28–41. DOI: https://doi.org/10.21776/ub.jurnalhpt. 2023. 011.1.4.
Thalib, R., Fernando, R., Khodijah, K., Meidalima, D. & Herlinda, S. (2013). Patogenisitas isolat Beauveria bassiana dan Metarhizium anisopliae asal tanah lebak dan pasang surut sumatera selatan untuk agens hayati Scirpophaga incertulas. Jurnal Hama dan Penyakit Tumbuhan Tropika, 13(1), 10–18. DOI: https://doi.org/10. 23960/j.hptt.11310-18.
Utami, R. S., Isnawati & Ambarwati, R. (2014). Eksplorasi dan karakterisasi cendawan entomopatogen Beauveria bassiana dari kabupaten malang dan magetan. LenteraBio, 3(1), 59–66.
Wang, H., Peng, H., Li, W., Cheng, P. & Gong, M. (2021). The toxins of Beauveria bassiana and the strategies to improve their virulence to insects. Frontiers in Microbiology, 12. DOI: https://doi.org/10.3389/fmicb.2021.705343