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May 25, 2025
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June 10, 2025
Published
June 11, 2025
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Abstract

Rice (Oryza sativa L.) is a staple food crop that plays a vital role in supporting national food security. One of the major constraints in rice cultivation is blast disease, caused by the fungal pathogen Pyricularia oryzae, which can significantly reduce crop productivity and lead to substantial economic losses. The conventional management of this disease relies heavily on chemical pesticides, which pose long-term risks to environmental and human health. Therefore, eco-friendly alternatives such as biological control agents are necessary, with Bacillus spp. being one promising candidate. This study aimed to evaluate the antagonistic activity of three Bacillus spp. isolates (Bcz-14, Bcz-20, and Bcz-30) against P. oryzae at two bacterial population densities (10⁶ CFU/mL and 10⁹ CFU/mL) under in vitro conditions. The experiment was conducted at the Plant Health Laboratory, Faculty of Agriculture, Universitas Pembangunan Nasional “Veteran” East Java, from January to March 2025. Results of the antagonism assay demonstrated that all Bacillus spp. isolates inhibited the growth of P. oryzae, with the highest inhibition observed in treatments Bcz-14 and Bcz-30 at 10⁶ CFU/mL, with inhibition rates of 24.29% and 23.35%, respectively. The observed inhibition mechanisms included competition and antibiosis. Microscopic observations revealed abnormal hyphal structures in P. oryzae, including lysis, swelling, and chlamydospore formation, in response to Bacillus spp. treatment.


 

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Author Biographies

Setiawan Setiawan, Fakultas Pertanian, Universitas Pembangunan Nasional “Veteran”Jawa Timur

Program Studi Agroteknologi

Yenny Wuryandari, Fakultas Pertanian, Universitas Pembangunan Nasional “Veteran”Jawa Timur

Program Studi Agroteknologi

Tr Mujoko, Fakultas Pertanian, Universitas Pembangunan Nasional “Veteran”Jawa Timur

Program Studi Agroteknologi

How to Cite
Setiawan, A. B., Wuryandari, Y., & Mujoko, T. (2025). KEMAMPUAN ANTAGONISTIK BEBERAPA ISOLAT Bacillus spp. TERHADAP PATOGEN Pyricularia oryzae PENYEBAB PENYAKIT BLAS TANAMAN PADI (Oryza sativa L.) PADA BEBERAPA KONSENTRASI. Jurnal Ilmu-Ilmu Pertanian Indonesia, 27(1), 67–76. https://doi.org/10.31186/jipi.27.1.67-76
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References

  1. Astriawati, F. & Anfa, Q. (2025). Isolasi dan karakterisasi fungi Pyricularia oryzae penyebab penyakit blas pada tanaman padi. Biospecies, 18(1), 16–24. DOI: https://doi.org/10.22437/biospecies.v18i1.38524.
  2. Bushal, K., & Neupane, N. (2021). A review of blast disease of rice in Nepal. Journal of Plant Pathology and Microbiology, 12, 1–5. DOI: https://doi.org/10.35248/2157-7471.20.12.528.
  3. Djojosumatro, P. (2008). Pestisida dan aplikasinya. Agromedia Pustaka, Jakarta.
  4. Elfina, Y., Sabirunah, A., Wijayanto, D., Rizqi, A. & Mu’arif, M. (2024). The morphological identification of fungi causing cocoa fruit rot disease and inhibition test of Bacillus spp. against the fungus in vitro. Jurnal Budidaya Pertanian, 20(1), 83–89. DOI: https://doi.org/10.30598/jbdp/2024.20.1.83.
  5. Hanif, A., Suryanto, D. & Nurwahyuni, I. (2012). Pemanfaatan bakteri kitinolitik dalam menghambat pertumbuhan Curvularia sp. penyebab penyakit bercak daun pada tanaman mentimun. Saintia Biologi, 1(1), 26–32.
  6. Hersanti, Safitri, N., Djaya, L. & Sianipar, M. S. (2020). Kemampuan Bacillus subtilis dan Trichoderma harzianum dalam campuran serat karbon dan silika nano untuk meningkatkan ke-tahanan tanaman padi terhadap penyakit blas (Pyricularia oryzae). Agrikultura, 31(3), 182. DOI: https://doi.org/10.24198/agrikultura. v31i3.29483.
  7. Khan, N., Martínez-Hidalgo, P., Ice, T. A., Maymon, M., Humm, E. A., Nejat, N., ... & Hirsch, A. M. (2018). Antifungal activity of Bacillus species against Fusarium and analysis of the potential mechanisms used in biocontrol. Frontiers in Microbiology, 9, 2363. DOI: https://doi.org/10.3389/fmicb.2018.02363.
  8. Kuswinanti, T., Patandjengi, B. & Amin, N. (2023). Pyricularia oryzae: Races distribution and screening of fungal antagonists in vitro. Pakistan Journal of Phytopathology, 35(1), 55–65. DOI: https://doi.org/10.33866/phytopathol.035.01.0848.
  9. Leiwakabessy, C., Inayatri, F., Jambormias, E., Patty, J. & Ririhena, R. E. (2020). Ketahanan enam varietas padi terhadap penyakit blas (Pyricularia oryzae Cav.) pada lahan sawah irigasi dan sawah tadah hujan. Jurnal Budidaya Pertanian, 16(2), 147–156. DOI: https://doi.org/ 10.30598/jbdp.2020.16.2.147.
  10. Lestari, S. A., Ramdan, E. P., Kulsum, U., Risnawati & Pribadi, E. M. (2021). Identifikasi penyebab penyakit blas padi pada kombinasi pola tanam System of Rice Intensification (SRI) dan jajar legowo. Agropross, National Conference Proceedings of Agriculture, Politeknik Negeri Jember. DOI: https://doi.org/10.25047/agropross.2021.235.
  11. Masyitah, N., Chamzurni, T. & Oktarina, H. (2023). Uji kompatibilitas kombinasi Bacillus thuringiensis dan Pseudomonas aeruginosa untuk mengendalikan Fusarium oxysporum pada pembibitan melon (Cucumis melo L.). Jurnal Ilmiah Mahasiswa Pertanian, 8(1), 466–482.
  12. Nabila, F. & Asri, M. T. (2021). Keefektifan Bacillus subtilis, Bacillus megaterium dan kombinasi Bacillus terhadap penghambatan pertumbuhan Ralstonia solanacearum secara in vitro. LenteraBio: Berkala Ilmiah Biologi, 10(2), 220–225. DOI: https://doi.org/10.26740/lenterabio.v10n2.p220-225.
  13. Novina, D. & Suryanto, D. (2013). Uji potensi bakteri kitinolitik dalam menghambat pertumbuhan Rhizoctonia solani penyebab rebah kecambah pada kentang varietas Granola. Saintia Biologi, 1(1), 26–32.
  14. Rochmawati, Z. N. & Trimulyono, G. (2020). Uji antagonis Bacillus subtilis dan Bacillus megaterium terhadap pertumbuhan Cercospora sp. yang diisolasi dari Nepenthes sp. LenteraBio: Berkala Ilmiah Biologi, 9(3), 204–210.
  15. Sandy, Y. A., Dewi, F. S. & Li’aini, A. S. (2024). Isolasi, identifikasi dan karakterisasi jamur Pyricularia oryzae penyebab penyakit blas pada tanaman padi di Kediri, Jawa Timur. Journal of Applied Agricultural Sciences, 8(2), 167–174. DOI: https://doi.org/10.25047/agriprima.v8i2.669.
  16. Setiaji, A., Annisa, R. R. R. & Rahmandhias, D. T. (2023). Bakteri Bacillus sebagai agen kontrol hayati dan biostimulan tanaman. Rekayasa, 16(1), 96–106. DOI: https://doi.org/10.21107/rekayasa.v16i1.17207.
  17. Sofiani, M., Djauhari, S. & Aini, L. Q. (2017). Pengaruh aplikasi Plant Growth Promoting Rhizobacteria (PGPR) dalam menghambat penyakit rebah kecambah yang disebabkan oleh jamur Sclerotium rolfsii pada kedelai. Jurnal Hama dan Penyakit Tumbuhan, 4(1), 32–38.
  18. Suganda, T., Yulia, E., Widiantini, F. & Hersanti, H. (2016). Intensitas penyakit blas (Pyricularia oryzae Cav.) pada padi varietas Ciherang di lokasi endemik dan pengaruhnya terhadap kehilangan hasil. Agrikultura, 27(3).
  19. Syofiana, R. V. T. & Masnilah, R. (2019). Eksplorasi Bacillus spp. pada beberapa patogen rizosfer gulma dan potensinya sebagai agens pengendali hayati patogen tanaman secara in vitro. Jurnal Bionindustri, 2(1), 344–363.
  20. Takagi, M., Kaku, K., Watanabe, S., Kawai, K., Shimizu, T., Sawada, H., Kumakura, K. & Nagayama, K. (2004). Mechanism of resistance to carpropamid in Magnaporthe grisea. Pest Management Science, 60, 921–926. DOI: https://doi.org/10.1002/ps.897
  21. Wahyuni, S. (2019). Isolasi dan karakterisasi Actinomycetes dari beberapa sentra perkebunan bawang antagonis Fusarium oxysporum f. sp cepae dan perkecambahan tanaman bawang merah (Allium ascalonicum L.) varietas Tuktuk [Disertasi, Universitas Negeri Makassar].
  22. Wicaksono, D., Arif, W., & Ani, W. (2017). Metode isolasi Pyricularia oryzae penyebab penyakit blas padi. Jurnal Hama dan Penyakit Tropika, 17(1), 62–69.
  23. Zakqy, N. (2023). Potensi agensi hayati Bacillus spp. dalam menghambat penyakit layu Fusarium serta pengaruhnya terhadap pertumbuhan dan produksi cabai rawit (Capsicum frutescens L.) [Skripsi, Universitas Pembangunan Nasional “Veteran” Jawa Timur].
  24. Zinidin, M. (2022). Eksplorasi Bacillus spp. pada rizosfer cabai merah (Capsicum annuum L.) dataran tinggi dan potensinya sebagai agensia pengendalian hayati patogen Ralstonia solanacearum secara in vitro [Skripsi, Universitas Pembangunan Nasional “Veteran” Jawa Timur].