Main Article Content
Abstract
[IDENTIFICATION OF SPECIES AND POPULATION OF ARBUSCULAR MYCORRHIZAL FUNGI IN SUGARCANE PLANTATIONS AT PG. REJO AGUNG BARU, MADIUN]. Arbuscular Mycorrhizal Fungi (AMF) play an important role in enhancing plant root capacity for nutrient uptake. This study aimed to identify the species and determine the population of AMF in sugarcane fields at PG. Rejo Agung Baru, Madiun. Observations were conducted on four fields with different productivity levels: very high, high, moderate, and low. The research employed a quantitative descriptive approach with an exploratory method. Soil and root samples were analyzed to determine AMF species and population density, while the relationships between AMF populations and soil properties were examined using simple linear regression and t-tests. The results revealed that all sites contained the same AMF species, Glomus sp., which exhibited a high adaptability to various soil fertility conditions. The highest AMF population was observed in the very high productivity field (1.01 × 10⁷ spores/ha), whereas the lowest was found in the moderate productivity field (0.06 × 10⁷ spores/ha). Population variations were influenced by interactions among soil physical and chemical properties, particularly pH, organic carbon, total nitrogen, and clay fraction. Overall, this study demonstrates that the occurrence and population density of AMF, especially Glomus sp., are strongly affected by soil conditions. Optimal AMF populations were associated with soils characterized by near neutral pH, high organic matter content, low clay fraction, and moderate nitrogen levels, suggesting that these factors are critical for enhancing AMF activity in sugarcane cultivation.
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References
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- Genre, A., Chabaud, M., Faccio, A., Barker, D. G. & Bonfante, P. (2008). Prepenetration apparatus assembly precedes and predicts the colonization patterns of arbuscular mycorrhizal fungi within the root cortex of both Medicago truncatula and Daucus carota. The Plant Cell, 20(5), 1407–1420.
- Gloria, S., Hasairin, A. & Edi, S. (2022). Mengenal spora mikoriza hutan kampus Universitas Negeri Medan berbasis literasi sains. Angewandte Chemie International Edition, 6(11), 951–952.
- Iba, Z. & Wardhana, A. (2024). Analisis regresi dan analisis jalur untuk riset bisnis menggunakan SPSS 29.0 & SMART-PLS 4.0 (1st ed.). Eureka Media Aksara.
- Lele, O. K. (2023). Peran endomikoriza dan tingkat ketersediaan air pada tanah Vertisol terhadap peningkatan serapan fosfor dan hasil paprika. Savana Cendana, 8(1), 1–5. DOI:https://doi.org/10.32938/sc.v8i01.1935.
- Masebo, N., Birhane, E., Takele, S., Belay, Z., Lucena, J. J., Pérez-Sanz, A. & Anjulo, A. (2023). Diversity of arbuscular mycorrhizal fungi under different agroforestry practices in the drylands of Southern Ethiopia. BMC Plant Biology, 23 (1), 634. DOI:https://doi.org/10.1186/s12870023-04645-6.
- Maulana, M., Ritaqwin, Z. & Mawaddah, F. (2022). Pertumbuhan dan kolonisasi fungi mikoriza terhadap cekaman tanah salin pada tanaman cabai. Jurnal Biologi Tropis, 16(1), 1–12.
- Maydayana, A., Kusumo, B. H., Bakti, L. A. A. & Dewi, R. A. S. (2023). Pengaruh pemberian biochar terhadap perubahan sifat kimia tanah Vertisol dan pertumbuhan kacang hijau (Vigna radiata L.): The impact of biochar application on alterations in chemical properties of Vertisol soil and the growth of mung bean (Vigna radiata L.). Jurnal Sains Teknologi & Lingkungan, 9(4), 663–674. DOI: https://doi.org/10.29303/jstl.v9i4.478.
- Mualif, M. S. & Kusumawati, A. (2021). Pengaruh sifat kimia tanah terhadap produktivitas tebu (Saccharum officinarum L.). Jurnal Pengelolaan Perkebunan (JPP), 2(2), 66–72. DOI:https://doi.org/10.54387/jpp.v1i1.5.
- Nugroho, W. A. & Prasetya, B. (2023). Eksplorasi mikoriza arbuskular pada beberapa sistem penggunaan lahan pertanian di Desa Ngawonggo, Kecamatan Tajinan, Kabupaten Malang. Jurnal Tanah dan Sumberdaya Lahan, 10(1), 25–35. DOI:https://doi.org/10.21776/ub.jtsl.2023.010.1.3.
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- Prayoga, M. H. & Prasetya, B. (2021). Eksplorasi mikoriza arbuskula indigenous pada rizosfer vegetasi lahan pascatambang batubara. Jurnal Tanah dan Sumberdaya Lahan, 8(2), 349-357. DOI: https://doi.org/10.21776/ub.jtsl.2021.008.2.6.
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- Sarah, S., Ab, B. & Bustan, B. (2024). Sebaran nilai kapasitas tukar kation (KTK) dan kemasaman (pH) tanah di tanah Vertisol Kecamatan Sakra Kabupaten Lombok Timur. Journal of Soil Quality and Management, 3(1), 1–6. https://doi.org/10.29303/jsqm.v3i1.145
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References
Alayya, N. P. & Prasetya, B. (2022). Kepadatan spora dan persen koloni mikoriza vesikula arbuskula (MVA) pada beberapa tanaman pangan di lahan pertanian Kecamatan Jabung Malang. Jurnal Tanah dan Sumberdaya Lahan, 9(2), 267–276. DOI:https://doi.org/10.21776/ub.jtsl.2022.009.2.7.
Alhudaya, S., Syafruddin & Marliah, A. (2023). Pengaruh jenis mikoriza dan kombinasi media tanam terhadap pertumbuhan bibit kakao (Theobroma cacao L.) pada tanah Entisol. Jurnal Ilmiah Mahasiswa Pertanian,7(2), 47
Aryanto, R., Rozy, M. F., Purwiyono, T. T. & Yulianti, R. (2022). Analisis laju infiltrasi berbagai penggunaan lahan. Indonesian Mining and Energy Journal, 5(1), 17–23. DOI:https://doi.org/10.25105/imej.v5i1.13777.
Bening Jiwa Jeni, K., Maroeto, Solekhah, B. A., Sholikah, D. H. & Rosmala, K. I. (2025). Kesesuaian lahan untuk komoditas tebu, matoa, vanili,dan jagung di wilayah Wonosalam, Jombang, Jawa Timur. Agroteknika,1(2),231–247.
Bouskout, M., ElJebri, S., Khan, Y., Saleh, I. A., Okla, M. K., Al-Amri, S. S., Ahmed, S., Dounas, H. & Ouahmane, L. (2025). Unveiling native mycorrhizal fungi diversity: Insights into growth performance, nutrient uptake, and root system robustness in caper-bush (Capparis spinosa L.) seedlings. Journal of Agriculture and Food Research, 21, 101984. DOI:https://doi.org/10.1016/j.jafr.2025.101984.
Burak, K., Yanardağ, İ. H., Gómez-López, M. D., Faz, Á., Yalçin, H., Sakin, E., Ramazanoğlu, E., Orak, A. B., & Yanardağ, A. (2024). The effect of arbuscular mycorrhizal fungi on biological activity and biochemical properties of soil under vetch growing conditions in calcareous soils. Heliyon, 10(3), e24820. DOI:https://doi.org/10.1016/j.heliyon.2024.e24820
Creswell, J. W. & Creswell, J. D. (2017). Research design: Qualitative, quantitative, and mixed methods approaches (5th ed.). SAGE Publications.
Dulur, N. W. D., Wangiyana, W., Farida, N. & Kusnarta, I. G. M. (2020). Growth and yield of soybean direct-seeded following conventional and aerobic rice intercropped with peanut and amended with organic wastes. International Journal of Horticulture, Agriculture and Food Science, 4(5), 189–195. DOI: https://doi.org/10.22161/ijhaf.4.5.2.
Fabilla, N., Astiko, W. & Fauzi, M. T. (2023). Pengaruh berbagai teknik pengolahan tanah yang ditambahkan bioamelioran terhadap pertumbuhan dan hasil tumpangsari jagung dan kedelai di lahan kering. E-Prints Unram, 1, 1–17.
Fall, A. F., Nakabonge, G., Ssekandi, J., Founoune Mboup, H., Apori, S. O., Ndiaye, A., Badji, A. & Ngom, K. (2022). Roles of arbuscular mycorrhizal fungi on soil fertility: Contribution in the improvement of physical, chemical, and biological properties of the soil. Frontiers in Fungal Biology, 3, 723892. DOI:https://doi.org/10.3389/ffunb.2022.723892.
Fatmawati, Z., Widada, J., Widianto, D. & Cahyaningtyas, A. (2025). Keragaman jamur mikoriza arbuskular pada rizosfer tiga varietas tanaman tebu. [Manuskrip tidak dipublikasikan].
George, N. P. & Ray, J. G. (2025). Arbuscular mycorrhizal fungal (AMF) diversity and population dynamics in organic vs chemicalized fields concerning soil types, agroclimatic zones and different organic farming practices over seasons, in South India. Geoderma Regional, 42, e00992. DOI: https://doi.org/10.1016/j.geodrs.2025.e00992.
Genre, A., Chabaud, M., Faccio, A., Barker, D. G. & Bonfante, P. (2008). Prepenetration apparatus assembly precedes and predicts the colonization patterns of arbuscular mycorrhizal fungi within the root cortex of both Medicago truncatula and Daucus carota. The Plant Cell, 20(5), 1407–1420.
Gloria, S., Hasairin, A. & Edi, S. (2022). Mengenal spora mikoriza hutan kampus Universitas Negeri Medan berbasis literasi sains. Angewandte Chemie International Edition, 6(11), 951–952.
Iba, Z. & Wardhana, A. (2024). Analisis regresi dan analisis jalur untuk riset bisnis menggunakan SPSS 29.0 & SMART-PLS 4.0 (1st ed.). Eureka Media Aksara.
Lele, O. K. (2023). Peran endomikoriza dan tingkat ketersediaan air pada tanah Vertisol terhadap peningkatan serapan fosfor dan hasil paprika. Savana Cendana, 8(1), 1–5. DOI:https://doi.org/10.32938/sc.v8i01.1935.
Masebo, N., Birhane, E., Takele, S., Belay, Z., Lucena, J. J., Pérez-Sanz, A. & Anjulo, A. (2023). Diversity of arbuscular mycorrhizal fungi under different agroforestry practices in the drylands of Southern Ethiopia. BMC Plant Biology, 23 (1), 634. DOI:https://doi.org/10.1186/s12870023-04645-6.
Maulana, M., Ritaqwin, Z. & Mawaddah, F. (2022). Pertumbuhan dan kolonisasi fungi mikoriza terhadap cekaman tanah salin pada tanaman cabai. Jurnal Biologi Tropis, 16(1), 1–12.
Maydayana, A., Kusumo, B. H., Bakti, L. A. A. & Dewi, R. A. S. (2023). Pengaruh pemberian biochar terhadap perubahan sifat kimia tanah Vertisol dan pertumbuhan kacang hijau (Vigna radiata L.): The impact of biochar application on alterations in chemical properties of Vertisol soil and the growth of mung bean (Vigna radiata L.). Jurnal Sains Teknologi & Lingkungan, 9(4), 663–674. DOI: https://doi.org/10.29303/jstl.v9i4.478.
Mualif, M. S. & Kusumawati, A. (2021). Pengaruh sifat kimia tanah terhadap produktivitas tebu (Saccharum officinarum L.). Jurnal Pengelolaan Perkebunan (JPP), 2(2), 66–72. DOI:https://doi.org/10.54387/jpp.v1i1.5.
Nugroho, W. A. & Prasetya, B. (2023). Eksplorasi mikoriza arbuskular pada beberapa sistem penggunaan lahan pertanian di Desa Ngawonggo, Kecamatan Tajinan, Kabupaten Malang. Jurnal Tanah dan Sumberdaya Lahan, 10(1), 25–35. DOI:https://doi.org/10.21776/ub.jtsl.2023.010.1.3.
Pangaribuan, E. A. S., Darmawati, A. & Budiyanto, S. (2020). Pertumbuhan dan hasil tanaman pakchoy pada tanah berpasir dengan pemberian biochar dan pupuk kandang sapi. Agrosains:Jurnal Penelitian Agronomi, 22(2), 72. DOI:https://doi.org/10.20961/agsjpa.v22i2.42093.
Prayoga, M. H. & Prasetya, B. (2021). Eksplorasi mikoriza arbuskula indigenous pada rizosfer vegetasi lahan pascatambang batubara. Jurnal Tanah dan Sumberdaya Lahan, 8(2), 349-357. DOI: https://doi.org/10.21776/ub.jtsl.2021.008.2.6.
Putra, T. K., Afany, M. R. & Widodo, R. A. (2020). Pengaruh bahan organik dan tanah Vertisol sebagai pembenah tanah terhadap ketersediaan dan pelindian kalium di tanah Regosol pasir pantai. Jurnal Tanah dan Air (Soil and Water Journal), 17(1), 20. DOI: https://doi.org/10.31315/jta.v17i1.4007.
Sarah, S., Ab, B. & Bustan, B. (2024). Sebaran nilai kapasitas tukar kation (KTK) dan kemasaman (pH) tanah di tanah Vertisol Kecamatan Sakra Kabupaten Lombok Timur. Journal of Soil Quality and Management, 3(1), 1–6. https://doi.org/10.29303/jsqm.v3i1.145
Sugiyono. (2018). Metode penelitian pendidikan: Pendekatan kuantitatif, kualitatif, dan R&D (Cetakan ke-27). Alfabeta.
Wahid, I. (2018). Keanekaragaman fungi mikoriza arbuskula di kawasan manifestasi geothermal Ie Jueseulawah Agam Desa Meurah Kecamatan Seulimeum sebagai referensi mata kuliah ekologi tumbuhan. Jurnal Biologi Edukasi, 6(1), 1–7.