Isi Artikel Utama
Abstrak
Bacterial wilt disease caused by Ralstolnia solanacearum had decreased the ginger production. Saphrophyte and antagonistic microbe are potential to control this disease by Integrated application. This microbes could be found at supressive land for pathogens. Research due to select the potential microbes to control bacterial wilt disease on ginger. Microbe was isolated from rhizosfer soil and root of healthy crop on infected ginger cropping by Ralstolnia solanacearum in Bengkulu by plate dilution methode using Pepton Glucose Agar (PGA) and Ginger-Potato Dextrose Agar (GPDA). Antagonistic was tested by Double Culture Technique on PGA and GPDA. Selected saphrophyte and antagonistic isolat was cultured on organic medium and tested to ginger crop that grown on infected soil. Disease development was observated until 5 months-age crop. The result of this experiment had isolated 4 fungi isolates and 4 bacteria isolates that potential as biocontrol agent to Ralstolnia solanacearum. Tested on Var. Badak ginger cropping indicated that all isolates had reduced 64-84% of diseases. Five Isolates of Pennicilium digitatum, Trichoderma harzianum, T. viride, Achromobacter sp., and Pseudomonas fluorescence was protected the crop to zero diseases; while three isolates of Trichoderma koningii, Bacillus sp., and Pseudomonas putida was protected the crop to 4% infected. Amundment of 12 isolates cultured on organic matter can improve the growth of false stem 11.11 – 96.97%; leave number 8.29 - 156%; and plant height 27.68 – 93.75%.
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Referensi
- Baker, K.F. and R.J. Cook. 1983. Biological Control of Plant Pathogen. Freeman dan Co. San Francisco.
- Bustamam, H. 1997. Patogenisitas dua belas isolat
- Pseudomonas solanacearum dan ketahanan beberapa klon jahe di bengklu. J. Penelitian UNIB (8) : 53-57
- Bustamam, H. 2001. Pengaruh pemberian jamur terhadap serapan P dan pengurangan penyakit layu bakteri pada tanaman jahe. J.Akta Agrosia 4(2):69-75.
- Bustamam, H. 2003. Perkembangan penelitian penyakit jahe di Bengkulu. Proseding Lokakarya Grand Design Pengembangan Jahe Sehat di Propinsi Bengkulu. Dinas Perkebunan Propinsi Bengkulu. 29 Juli 2003.
- Bustamam, H., D. Apriyanto, dan E. Inoriah. 2003. Pedoman Budidaya Jahe Sehat Untuk Penangkaran Benih Jahe. Dinas Perkebunan
- Propinsi Bengkulu.
- Butt, T.M., C. Jackson and N. Magan. 2001. Introduction – Fungal Biological Control Agents: Progress, Problems, and Potential. In Butt, T.M., C. Jackson and N. Magan : Fungi as Biocontrol Agents. CAB International : 1-8
- Dinas Perkebunan dan Kehutanan Kabupaten Rejang Lebong, 2003. Laporan Tahunan. Dinas Perkebunan dan kehutanan Kabupaten Rejang Lebong. Curup.
- Domsch, K.H, W. Gams, and T.H. Anderson. 1980. Compendium of soil fungi. Academic Press. London.
- Hartati, S.Y. and Supriadi. 1994. Systemic action of bactericide containing oxytetracycline and streptomycin sulphate in treated ginger rhizomes. J. of Spice and Medicine Crops 3
- :7-11
- Hartman, G.L., W.F. Fong, Hanudin, and A.C. Hayward. 1993. Potential of biological and chemical control of bacterial wilt in : Hartman, G.L. and A.C. Hayward (Eds.) Bacterial Wilt. ACIAR Proceddings 45:322- 326
- Howell, C.R., L.E. Hanson, R.D. Stipanovic and
- S.L. Puckhaber. 2000. Induction of trepenoid synthesis in cotton roots and control of Rhizoctonia solany by seed treatment with Trichoderma virens. Phytopathology 90(3)248-252
- Januwati, M. 1999. Optimalisasi ushatani tanaman jahe. Balai Peneneltian Tanaman Rempah dan Obat.
- Meyer, J.M., D. Hohnadel, and F.Halle. 1989. Cephabactin from Pseudomonas cepacia, a new type of siderophore. J.Gen. Microbiol. 135:1479-1487.
- Mulya, K., M. Wanatabe, M. Goto, Y. Takikawa and S. Tsuyumu, 1996. Supression of bacterial wilt diseases of tomato by root dipping with Pseudomonas fluorescens PfG32: The role of antibiotic and siderophore production. Ann. Phytopath. Soc. Japan 62(2):134-140
- Mulya, K. Supriadi, E.M. Adhi, S. Rahayu dan
- N. Karyani.2000. Potensi bakteri antagonis
- dalam menekan perkembangan penyakit layu bakteri jahe. Jurnal LITRI 6(2):37-43
- Phae, C.G., M. Shoda, N.Kita, K. Nakano , and
- K. Ushiyama. 1992. Biological control of crown and root rot and bacterial wilt of tomato by Bacillus subtilis NB22. Ann.Pyhtopath.Soc.Japan 58:329-339
- Schaad, N.W., J.B. Jones dan W. Chun. 2001. Plant Pathogenic Bacteria. APS Press. St. Paul. Minnesota.
- Schekhawat, G.S., S.K. Chakrabarti, V. Kishore,
- V. Sunaina and A.V. Gadewar. 1993. Possibilities of biological management of potato bacterial wilt wilth strains of Bacillus sp., B. subtilis, Pseudomonas fluorescens, and actinomycetes. In : Hartman, G.L. and A.C. Hayward (Eds.) Bacterial Wilt. ACIAR Proceddings 45:327-330
- Schidler, D.A., P.J. Glininger and R.J. Bothast. 1997. Effect of antagonist cell concetration and two strain mixture on biological control of Fusarium dry rot of potatoes. Pyhtopatology 87: 177-183
- Semangun, H. 2000. Penyakit-penyakit tanaman hortikultura di Indonesia. Gadjah Mada University Press. Yogyakarta
- Setyowati, N., H. Bustamam, and T. Nopiyanti. 2003. Effect of microbes fertilizer on Lettuce (Lactuca sativa L.) yield, root disease, and weed growth. Proceding of International Seminar on Organic Farming and Sustainable Agriculture in The Tropic and Subtropic, Palembang October 8-9 : 67-72
- Subba-Rao, N.S. 1994. Mikroorganisme Tanah dan Pertumbuhan Tanaman. UI Press, Jakarta Utama, S.P., H. Bustamam, K.S. Hindarto,
- W. Marsigit, dan Fahrurozi. 2003. Analisis empat komoditas Jahe, tomat, aren dan jagung. Dinas Perindustrian Propinsi
- Bengkulu, Bengkulu
Referensi
Baker, K.F. and R.J. Cook. 1983. Biological Control of Plant Pathogen. Freeman dan Co. San Francisco.
Bustamam, H. 1997. Patogenisitas dua belas isolat
Pseudomonas solanacearum dan ketahanan beberapa klon jahe di bengklu. J. Penelitian UNIB (8) : 53-57
Bustamam, H. 2001. Pengaruh pemberian jamur terhadap serapan P dan pengurangan penyakit layu bakteri pada tanaman jahe. J.Akta Agrosia 4(2):69-75.
Bustamam, H. 2003. Perkembangan penelitian penyakit jahe di Bengkulu. Proseding Lokakarya Grand Design Pengembangan Jahe Sehat di Propinsi Bengkulu. Dinas Perkebunan Propinsi Bengkulu. 29 Juli 2003.
Bustamam, H., D. Apriyanto, dan E. Inoriah. 2003. Pedoman Budidaya Jahe Sehat Untuk Penangkaran Benih Jahe. Dinas Perkebunan
Propinsi Bengkulu.
Butt, T.M., C. Jackson and N. Magan. 2001. Introduction – Fungal Biological Control Agents: Progress, Problems, and Potential. In Butt, T.M., C. Jackson and N. Magan : Fungi as Biocontrol Agents. CAB International : 1-8
Dinas Perkebunan dan Kehutanan Kabupaten Rejang Lebong, 2003. Laporan Tahunan. Dinas Perkebunan dan kehutanan Kabupaten Rejang Lebong. Curup.
Domsch, K.H, W. Gams, and T.H. Anderson. 1980. Compendium of soil fungi. Academic Press. London.
Hartati, S.Y. and Supriadi. 1994. Systemic action of bactericide containing oxytetracycline and streptomycin sulphate in treated ginger rhizomes. J. of Spice and Medicine Crops 3
:7-11
Hartman, G.L., W.F. Fong, Hanudin, and A.C. Hayward. 1993. Potential of biological and chemical control of bacterial wilt in : Hartman, G.L. and A.C. Hayward (Eds.) Bacterial Wilt. ACIAR Proceddings 45:322- 326
Howell, C.R., L.E. Hanson, R.D. Stipanovic and
S.L. Puckhaber. 2000. Induction of trepenoid synthesis in cotton roots and control of Rhizoctonia solany by seed treatment with Trichoderma virens. Phytopathology 90(3)248-252
Januwati, M. 1999. Optimalisasi ushatani tanaman jahe. Balai Peneneltian Tanaman Rempah dan Obat.
Meyer, J.M., D. Hohnadel, and F.Halle. 1989. Cephabactin from Pseudomonas cepacia, a new type of siderophore. J.Gen. Microbiol. 135:1479-1487.
Mulya, K., M. Wanatabe, M. Goto, Y. Takikawa and S. Tsuyumu, 1996. Supression of bacterial wilt diseases of tomato by root dipping with Pseudomonas fluorescens PfG32: The role of antibiotic and siderophore production. Ann. Phytopath. Soc. Japan 62(2):134-140
Mulya, K. Supriadi, E.M. Adhi, S. Rahayu dan
N. Karyani.2000. Potensi bakteri antagonis
dalam menekan perkembangan penyakit layu bakteri jahe. Jurnal LITRI 6(2):37-43
Phae, C.G., M. Shoda, N.Kita, K. Nakano , and
K. Ushiyama. 1992. Biological control of crown and root rot and bacterial wilt of tomato by Bacillus subtilis NB22. Ann.Pyhtopath.Soc.Japan 58:329-339
Schaad, N.W., J.B. Jones dan W. Chun. 2001. Plant Pathogenic Bacteria. APS Press. St. Paul. Minnesota.
Schekhawat, G.S., S.K. Chakrabarti, V. Kishore,
V. Sunaina and A.V. Gadewar. 1993. Possibilities of biological management of potato bacterial wilt wilth strains of Bacillus sp., B. subtilis, Pseudomonas fluorescens, and actinomycetes. In : Hartman, G.L. and A.C. Hayward (Eds.) Bacterial Wilt. ACIAR Proceddings 45:327-330
Schidler, D.A., P.J. Glininger and R.J. Bothast. 1997. Effect of antagonist cell concetration and two strain mixture on biological control of Fusarium dry rot of potatoes. Pyhtopatology 87: 177-183
Semangun, H. 2000. Penyakit-penyakit tanaman hortikultura di Indonesia. Gadjah Mada University Press. Yogyakarta
Setyowati, N., H. Bustamam, and T. Nopiyanti. 2003. Effect of microbes fertilizer on Lettuce (Lactuca sativa L.) yield, root disease, and weed growth. Proceding of International Seminar on Organic Farming and Sustainable Agriculture in The Tropic and Subtropic, Palembang October 8-9 : 67-72
Subba-Rao, N.S. 1994. Mikroorganisme Tanah dan Pertumbuhan Tanaman. UI Press, Jakarta Utama, S.P., H. Bustamam, K.S. Hindarto,
W. Marsigit, dan Fahrurozi. 2003. Analisis empat komoditas Jahe, tomat, aren dan jagung. Dinas Perindustrian Propinsi
Bengkulu, Bengkulu