Konservasi Hayati

Characterization of Lactic Acid Bacteria (LAB) from Tempeh Probiotic Drink with Combination of Dates and Skim Milk

2024-03-12T22:45:23+00:00

Tempeh can be processed into a probiotic drink because it contains Lactic Acid Bacteria (LAB), which are beneficial for the digestive system. A combination of dates and skim milk as prebiotics is required by LAB as a substrate to produce energy and cellular components, and to increase the population to produce sufficient amounts of acid. This study aims to describe the characteristics of LAB present in tempeh probiotic drinks with a combination of dates and skim milk. Isolation was performed using the total plate count (TPC) method with the spread plate technique. Selected isolates were identified macroscopically, microscopically, biochemically, and physiologically. The results showed that the average amount of LAB viable counts was 9.6 x 10⁶ CFU/mL with 8 suspected isolates as LAB. Microscopic observation obtained all 8 isolates were Gram-positive with two round shapes and six-rod shapes. The catalase test results showed all 8 isolates were negative due to the absence of bubbles. The triple sugar iron agar (TSIA) test showed a yellow color, indicating the capability to ferment glucose, lactose, and sucrose. All selected isolates non-motile and positive MR and negative SCA testing. Some of the isolates showed tolerance to salts and acids based on physiological testing. The six most promising LAB isolates showed important characteristics of LAB that should be evaluated when selecting probiotic candidates, namely TEa-4130, TEb-4230, TEb-4130, TEb-3150, TEb-3250, and TEb-4250 isolates.

Inventarisasi Anggrek (Orchidaceae) di Kawasan Taman Wisata Alam Bukit Tangkiling Kalimantan Tengah

2024-02-07T08:38:13+00:00

Orchid (Orchidaceae) is one among the plants that grow in Taman Wisata Alam (TWA) Bukit Tangkiling, Central Kalimantan. The lack of information about orchid species in that area makes an inventory crucial. The purpose of this study was to inventory the species of orchids in the TWA Bukit Tangkiling area, as well as to learn about the environmental characteristics and distribution patterns of these plants. The research was conducted from October 2022 to March 2023. This research used the exploratory method and the sample was taken using the purposive sampling technique. Seven orchid species were identified as a result of this study: Acriopsis sp., Arachnis sp., Cymbidium finlaysonianum, Dendrobium crumenatum, Bulbophyllum purpurascens, Bromheadia finlaysoniana, and Dipodium pictum. Five species grow on plant stems (epiphytic), while two species grow on soil (terrestrial). Since each species was discovered less than 100 meters above sea level, they were all categorized as lowland orchid species. Dendrobium crumenatum is distributed randomly. The distribution of Bromheadia finlaysoniana, Bulbophyllum purpurascens, and Cyimbidium finlaysonianum is clustered. While the distribution pattern of the rest could not be determined because only one individual was found.

Inventarisasi Jamur Terbawa Benih Cabai Dari Desa Sidodadi Ramunia, Deli Serdang Sumatera Utara

2024-04-22T03:20:52+00:00

Inventory of seed-borne fungi is a data collection on fungi that infect seeds, this is done to determine the health of the seeds. Seed health testing is very necessary to prevent the spread of disease in the field through seeds. The aim of this research is to inventory and identify fungi carried by chili seeds from Sidodadi Ramunia village. The research method used was isolation of seed-borne fungi which refers to seed health testing methods, namely the Blotter Test method, Rolling Paper method and identification of the fungi obtained to determine the type of fungi that infects chili seeds. The results obtained were that chili seeds from Sidodadi Ramunia Village were infected with seed-borne fungi with the highest percentage being 8% and the lowest being 0.5%. The seed-borne fungi with the highest percentage of infection is the fungus Curvularia sp. The conclusion of this research is that red chili seeds from Sidodadi Ramunia Village, Deli Serdang, North Sumatra were infected with seed-borne fungi, namely Aspergillus sp1., Fusarium sp., Curvularia sp., and Aspergillus sp2. Next, it is necessary to carry out pathogenicity tests and molecular identification of the fungi that have been identified.

Kepadatan dan Estimasi Populasi Owa (Hylobates funereus) di Hutan Long Ayap, Kabupaten Berau, Provinsi Kalimantan Timur

2023-11-14T08:11:10+00:00

Gibbon (Hylobates funereus) is one of the endemic primates of Borneo Island. H. funereus is declared as endangered species in the IUCN Red List 2020. One of the threats is the loss of habitat caused by deforestation for palm oil plantations. The aim of the research is to assess population density and to estimate population of gibbons at Long Ayap Forest, Berau Regency, East Kalimantan Province. Observation is conducted using strip transect sampling method, carried out at three forest blocks at palm oil plantation of PT Mulia Inti Perkasa (MIP) from September to December 2022 with the total of 27 repetitions. The total gibbon population density is 11-11,96 individuals/km2. Estimation of gibbon population at Long Ayap Forest, Berau Regency, East Kalimantan Province is 17.82 individuals.