Article Sidebar

Submitted
October 17, 2020
Accepted
March 28, 2021
Published
June 15, 2021
Download
PDF
Statistic
Read Counter : 2083 Download : 1152

Downloads

Download data is not yet available.

Main Article Content

Abstract

[TUNGRO DISEASE ON RICE PLANT (Oryza sativa) IN TABA PENANJUNG DISTRICT: DISEASE INCIDENCE AND MOLECULAR DETECTION OF VIRUSES]. Tungro is one of the important diseases of rice plants. Tungro disease caused by Rice tungro bacilliform virus (RTBV) and Rice tungro spherical virus (RTSV). This research aims to obtain information on the spread of the disease, vector population, and molecular detection by polymerase chain reaction method. This research was conducted from December 2019 to April 2020 in rice fields in Taba Penanjung sub-district, Bengkulu Tengah district at ± 540 meters above sea level and virus detection in the Laboratory of Biotechnology and Genetics Department of Biology Faculty of Mathematics and Natural Sciences (MIPA) and Laboratory of Plant Protection, Agriculture Faculty, Bengkulu University. Data analysis used descriptive analysis. The results showed that the highest disease incidence on the seven weeks after planting (wap) is 1.7% and the highest population of the vector on the 7 wap with an average of 7.7 vector/100m2 in the village of Taba Penanjung. Detection of viruses with the PCR method with the specific primer DAF/DAR succeeded in amplification of DNA band measuring 1,400 bp. RTSV was not detected by RT- PCR using a specific primer or RTSV. The result of this detection shows that the symptoms of tungro on rice plants in the cultivation of Taba Penanjung district associated with RTBV infection. RTSV unable to detect can be caused by many factors in the detection process, including total RNA extraction, cDNA synthesis, DNA amplification, and DNA visualization of PCR products.

 

Article Details

License

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
How to Cite
Fiddin, A., Sutrawati, M., Bustamam, H., Ganefianti, D. W., & Sipriyadi, S. (2021). PENYAKIT TUNGRO PADA TANAMAN PADI (Oryza sativa) DI KECAMATAN TABA PENANJUNG : INSIDENSI PENYAKIT DAN DETEKSI VIRUS SECARA MOLEKULER. Jurnal Ilmu-Ilmu Pertanian Indonesia, 23(1), 37–45. https://doi.org/10.31186/jipi.23.1.37-45
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX

References

  1. Badan Meteorologi, Klimatologi, dan Geofisika (BMKG). (2020). Prakiraan cuaca rata-rata suhu harian, kelembapan harian, dan curah hujan harian di pos Kecamatan Taba Penanjung. Badan Meteorologi Klimatologi,dan Geofisika Provinsi Bengkulu, Bengkulu.
  2. Balai Besar Penelitian Tanaman Padi (BB Padi). (2020). Informasi :Varietas Padi Berdasarkan Tahun Pelepasan. http//bbpadi.litbang. pertanian.go.id/index. php/varietas-padi/inbrida-padi-sawah- inpari . 18 Juli 2020.
  3. BPS Provinsi Bengkulu. (2015a). Luas Panen Padi Sawah dan Padi Ladang Provinsi Bengkulu. Badan Pusat Statistik Provinsi Bengkulu, Bengkulu.
  4. BPS Provinsi Bengkulu. (2015b). Produksi Padi dan Palawija Provinsi Bengkulu. Badan Pusat Statistik Provinsi Bengkulu, Bengkulu.
  5. Baehaki, S.E. (1992). Berbagai Hama Serangga Tanaman Padi, Angkasa, Bandung.
  6. Azzam, O. & Chancellor, T.C.B. 2002). The Biology, Epidemiology, and Management of Rice Tungro Disease. Plant Disease, 86, 88-100.
  7. Cabauatan, P.Q. & Hibino, H. (1988). Isolation, purification, and serology of Rice Tungro bacilliform and Rice Tungro Spherical viruses. Plant Diseases, 72, 526-528.
  8. Davidson, J.G. (1923). Biological studies of Aphids rumicis Linn. The penetration of plant tissue and the source of the food supply of aphids. Annals of Applied Biology, 35-54.
  9. Doyle, J.J. & Doyle, J.L. (1990). A rapid total DNA preparation procedure for fresh plant tissue. Focus , 12, 13-15.
  10. Favali, M.A.,Pellegrini, S. & Bassi, M. (1975). Ultra structural alterations induced by tungro virus in rice leaves. Virology , 66, 502-507.
  11. Ladja, F.T. (2013). Gulma inang virus tungro dan kemampuan penularannya ke tanaman padi. Jurnal Penelitian Pertanian Tanaman Pangan, 32(3),187-191. DOI: http://dx.doi.org/10.21082/ jpptp.v32n3.2013.p187-191.
  12. Ling, K.C. (1966). Nonpersintence of the tungro virus of rice inits leafhopper vector, Nephotettix impicticeps. Phytopathology, 56, 1252-1256.
  13. Meidiwarman.(2008). Perkembangan populasi wereng hijau (Nephotettix sp.) pada beberapa varietas padi unggul nasional di musim hujan. Agroteksos , 18(1), 15-19.
  14. Muhsin, M. & Widiarta, I.N. (2009). Patosistem, strategi, dan komponen teknologi pengendalian tungro pada tanaman padi. Iptek Tanaman Pangan, 4(2), 202-221.
  15. Rivai, F. (2005). Dasar-Dasar Epidemiologi Penyakit Tumbuhan. Yayasan Perguruan Tinggi Komputer UPI PRESS., Padang.
  16. Sari, D. (2013). Uji ketahanan beberapa varietas padi terhadap virus tungro. DOI: http://dx. doi.org/10.23960/jat.v1i3.2060.
  17. Semangun, H. (2008). Penyakit-penyakit tanaman pangan di Indonesia. 2nd Ed. Gadjah Mada University Press., Yogyakarta.
  18. Suarsana, M., Parmila, I.P., Wahyuni, P.S. & Suarmika, I.G.M. (2020). Pengaruh serangan hama penggerek batang dan penyakit tungro terhadap produktivitas sembilan varietas padi di Loka Paksa, Bali. Agricultural Journal, 3(1), 84-90.DOI: https://10.37637/ab.v3i1. 461.
  19. Sutrawati, M., Sariasih Y, Priyatiningsih, Ladja, F.T. (2019). Deteksi virus yungro pada padi di Bengkulu. Jurnal Ilmu-Ilmu Pertanian Indonesia, 21(2),99-102.DOI:https://doi.org/ 10.31186/jipi.21.2.99-102
  20. Suzuki, Y., Widrawan, I.K.R., Gede, I.G.N., Raga, I.N., Yasis, & Soeroto. (1992). Field epidemiology and forecasting technology of rice tungro disease vectored by green leafhopper. Japan Agricultural Research Quarterly , 26, 98-104.
  21. Takahashi, Y., Tiongco, E.R., Cabauatan, P.Q., Koganezawa, H., Hibino, H. & Omura, T. (1993). Detection of rice tungro bacilliform virus by polymerase chain reaction for assessing mild infection of plants and viruliferous vector leafhoppers. Phytopathology, 83(6), 655-659.
  22. Widiarta, I.N., Kusdiaman, D. & Hasanuddin, A. (2003). Pemencaran wereng hijau dan keberadaan tungro pada pertanaman padi dengan beberapa cara tanam. Penelitian Pertanian Tanaman Pangan, 22, 129-133.
  23. Widiarta, I.N. (2005). Wereng hijau (Nephotettix virescens Distant): dinamika populasi dan strategi pengendaliannya sebagai vektor penyakit tungro. J Litbang Pertanian, 24(3), 85-92