Main Article Content

Abstract

[ELIMINATION OF Pineapple Mealybug Wilt-associated Virus (PMWaV) FROM PINEAPPLE PLANT BY HOT WATER TREATMENT]. Mealybug wilt of pineapple (MWP) is a devastating disease found in all the major pineapple growing regions. Closterovirus particles can be detected in both MWP symptomatic and asymptomatic pineapple. Rogue of symptomatic plants is the simplest method of controlling MWP disease, but this method is less effective as PMWaV might remains at asymptomatic plants. Use of pesticide to control mealybug and ants is not efficient and high cost. The research was conducted to develop elimination method for PMWaV-free plant by hot water treatment. PMWaV infected plant parts (leave, stem, crown) were exposed to two hot water treatments, consisting of 35 ºC for 24 hour as pre-treatment and immediately followed by hot water treatment either 56 ºC for 60 minute or 58 ºC for 40 minute in a water bath. The infected plant without hot water treatment served as the positive control, and healthy plant without hot water treatment as the negative control. PMWaV infection could be eliminated from propagative material through hot water treatment 58 ºC 40 minute in a water bath without decrease propagative material viability.

Article Details

How to Cite
Sutrawati, M., Suastika, G., & Sobir, S. (2017). ELIMINASI PINEAPPLE MEALYBUG WILT-ASSOCIATED-VIRUS (PMWaV) DARI TANAMAN NANAS DENGAN HOT WATER TREATMENT. Jurnal Ilmu-Ilmu Pertanian Indonesia, 12(1), 19–25. https://doi.org/10.31186/jipi.12.1.19-25

References

  1. Bartholomew. D.P., R.E. Paul, and K.G. Rohrbach. 2003. Pineapple: Botany, Production, and Uses. CAB international.
  2. Beardsley, J.W. 1996. The pineapple mealybug complex: taxonomy, distribution and host relationship. Acta Hort. 334: 383-386.
  3. Borroto F.E.G, A.J.A. Torres, and M. Laimer. 2007. RTPCR detection and protein-protein interaction of viral component of pineapple mealybug wilt-associated virus-2 in Cuba. Plant Pathology. 89: 435-439.
  4. [CABI] Centre in Agricultural and Biological Institute. 2005. Plant protection compendium. CAB International. Wallingford, United Kingdom.
  5. Deptan. 2008. Profil Nenas di Kabupaten Subang. http:// www.deptan.go.id [23 Maret 2007].
  6. Forsberg, G. 2001. Heat sanitation of cereal seeds with a new, efficient, cheap and environmentally friendly method. In: A.J. Biddle (ed). Seed Treatment, Challenges and Opportunities. Proceedings from Symposium No. 76 of the British Crop Protection Council BCPC, Farnham. pp. 69-72.
  7. Glasa, M.,G. Labonne, and J.B. Quiot. 2003. Effect of temperature on plum pox virus infection [abstract]. Acta Virol 47(1): 49-52. http://www. ncbi.nlm.nih.gov/ entrez/query.fcgi [13 April 2007].
  8. Hadidi, A., R.K. Khetarpal, and H. Koganezawa. 1998. Plant Virus Diseases Control. USA-APS.
  9. Hutahayan, A.J. 2006. Peranan strain dan Pineapple Mealybug Wilt-associated Virus dan kutu putih (Dysmicoccus spp.) dalam menginduksi gejala layu pada tanaman nanas. Thesis. Sekolah Pascasarjana IPB. Bogor.
  10. Hu, J.S., D.M. Sether, X.P. Liu, and M. Wang. 1997. Use of tissue blotting immunoassay to examine the distribution of pineapple closterovirus in Hawaii. Phytopathology 88: 1150-1154
  11. [PKBT] Pusat Kajian Buah Tropika. 2008. Perbanyakan Massal Bibit Nenas dengan Stek Daun. Pusat Kajian Buah Tropika, LPPM. Institut Pertanian Bogor. Rohrbach, K.G., J.W. Beardsley, T.L. German,
  12. N.J. Reimer, and W.G. Sanford. 1988. Mealybug wilt, mealybug, and ants of pineapple. Plant Disease 72: 558-565. Sether, D.M, A.V.
  13. Karasev, C. Okumura, C. Arakawa, F. Zee, M.M. Kislan, J.L.
  14. Busto, and J.S. Hu. 2001. Differentiation, distribution, and elimination of two different pineapple mealybug wilt-associated viruses found in pineapple. Plant Disease 85: 856-864. Sether, D.M. and
  15. J.S. Hu. 2002a. Closterovirus Infection and Mealybug Exposure are Necessary for the Development of Mealybug Wilt of Pineapple Disease. Phytopathology 92: 928-935.
  16. Sether, D.M. and J.S. Hu. 2002b. Yield impact and spread of pineapple mealybug wilt-associated virus-2 and mealybug wilt of pineapple in Hawaii. Plant Diseases 86: 867-874.
  17. Sether, D.M., D.E. Ullman, and J.S. Hu. 1998. Transmission of pineapple mealybug wilt-associated virus by two species of mealybug (Dysmicoccus Spp.). Phytopathology 88: 1224-1230.
  18. Tryono, R. 2006. Deteksi dan identifikasi Pineapple Mealybug Wilt-associated Virus penyebab penyakit layu pada tanaman nanas di Indonesia. Tesis. Sekolah Pascasarjana IPB. Bogor.