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Abstract
The objective of this experiment was to determine the optimum concentration of 6- benzyl amino purine (BAP) and 1-naphthalene acetic acid (NAA) for in vitro regeneration of diseases-free plantlets of ginger. Meristem part of ginger rhizome selected from infected area was used as plant material. The explant was cut into 0.5 cm3 and, then, cultured in an MS basal medium supplemented with combination of treatments. The experiment was
arranged in Completed Randomized Design with factorial arrangement of the treatments. The first factor was BAP concentrations (0,1,2,3,4 and 5 ppm of BAP), and the second factor was NAA concentrations (0,1,2,3,4 and 5 ppm of NAA). The experiment was done in 18 sterilization methods and planting techniques to regenerate disease-free plantlets. The results showed that supplements of BAP and NAA affectedin vitro plantlet regeneration of ginger. Additional BAP and NAA on the basal media improved percentage of root, shoot and plantlet formation about 83.33- 100%. The highest number of shoot was obtained on 4 ppm of BAP and 3 ppm of NAA, while the highest number of root was obtained on media with addition 3.58 ppm of BAP and 5 ppm of NAA. Supplementing the medium with 3-4 ppm of BAP and 3 ppm of NAA was the best combination for regenerating plantlets of ginger. Microscopic test of micro-rhizome cell showed that there was only 2% of sample infected by P. solanacearum. This result indicated that in vitro technique could be used as an alternative method to produce a disease-free rhizome of ginger.
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References
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References
Ammirato, P.V. 1986. Control and expression of morphogenesis in culture. p. 23-40. in : Withers, L.A and P.G. Alderson (eds). Plant tissue culture and its agricultural application, Butterworths Universirty Pres, Cambridge.
George, E.F and P.D Sherrington. 1984. Plant Propagation by Tissue Culture. Handbook and Directory of Commercial Laboratories. Exegetis. Ltd, England.
Halperin, W. 1978. Alternative morphogenic events in cells suspensions. Am. J.Bot. 53: 443-453
Hartman, G.L., W.F Fong, Hanudin, and A.C. Hayward. 1993. Potential of biological and chemical control of bacterial wilt. In : Hartman G.L. and A.C. Hayward (Eds.) Bacterial Wilt. ACIAR Proceedings No. 45 : 322-326.
Hosoki, T and Y. Sagawa. 1977. Clonal propagation of ginger (Zingiber officinale Roscoe.) through tissue culture. HortScience. 12(5):451-452.
Krikorian, A.D., K.Kelly, and D.L Smith. 1987. Hormones in tissue culture and micropropagation.p.593-613.in P.J.Davies (ed). Plant hormone and their role in plant growth and development. Martinus Nijhoff Publishers. Dordrecht, Netherlands.
Marlin. 1998. High Multiplication of Plant Regeneration of Garlic (Allium sativum L.) in vitro. J.Akta Agrosia 2(2):57-60.
Marlin, Alnopri dan A. Rohim. 2000. Proliferasi tunas jahe (Zingiber officinale Rosc.) in vitro dengan pemberian sukrosa dan agar powder. J.Akta Agrosia 4(2) : 45-47.
Salisburry, F.B. and C.W, Ross. 1992. Plant Physiology. 4th edition. Wadsworth Publ. Diterjemahkan oleh Lukman, D.R dan Sumaryono. 1995. Fisiologi Tumbuhan. Jilid 3. ITB, Bandung.
Simatupang, S. 1996. Pengaruh penambahan sitokinin dan asam naphthalene acetic acid pada media Murashige and Skoog terhadap perkembangan eksplan asparagus. J.Hort 6(2) : 105-108.
V
an, T.T.K and T.H. Trinh. 1990. Organic Differentiation. In; S.S. Bhojwani (ed.). Plant Tissue Culture: Applications and Limitations. Elsevier Science Publ, Netherlands.
Wareing, P.F. and I.D.J. Phillips. 1981. Growth and differentiation in Plants. Pergamon Press 3rd Ed.
Wattimena, G.A. 1987. Zat Pengatur Tumbuh Tanaman. PAU. Bioteknologi. IPB, Bogor.
Weiss, E.A. 1997. Essential Oil Crops. Cab International, New York.
Zulparmaidi.1993. Karakteristik dan patoagenitas beberapa isolate bakteri P. solanacearum, penyebab panyakit layu bakteri pada tanaman jahe. Skripsi Jurusan Budidaya Pertanian, Fakultas Pertanian, Universitas Bengkulu, Bengkulu. (Tidak dipublikasikan)