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Abstract

The research aim was to determine the optimum ratio and flow rate of mobile phase of acetonitrile: water for separation of pesticide residue of imidacloprid, profenofos and deltamethrin simultaneously with HPLC. The compromise wavelength of the three of residues was measured with UV–VIS spectrophotometer. The ratio of acetonitrile:water consisted of 70 : 30, 60 : 40, 50 : 50, 40 : 60 and 30 : 70 v/v, and the flow rate of the mobile phase consisted of 0,5, 0,75 and 1 mL minutes-1, respectively. The results show the optimum mobile phase was achieved at a ratio of 60:40 v/v and at a wavelength of 270 nm with retention tim of imidacloprid, profenofos and deltamethrin components achieved at 3.0, 6.2 and 17.1 minutes, respectively. The higher ratio of acetonitrile: water and higher mobile phase resulted in accelerating its retention time and a good separation of imidacloprid was not achieved. An unstatisfied separation was also obtained when smaller ratio of acetonitrile: water and lower mobile phase was used. It occurred due to higher deltametrin retention time.

 

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How to Cite
Nurhamidah, N. (2018). PENENTUAN KONDISI OPTIMUM HPLC UNTUK PEMISAHAN RESIDU PESTISIDA IMIDAKLOPRID, PROFENOFOS DAN DELTAMETRIN PADA CABAI (Capsicumannum ). Jurnal Ilmu-Ilmu Pertanian Indonesia, 7(2), 87–93. https://doi.org/10.31186/jipi.7.2.87-93
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References

  1. Deptan. 1997a. Metode Pengujian Residu Pestisida Dalam Hasil Pertanian. Komisi Pestisida Departemen Pertanian, Jakarta.
  2. Deptan 1997b. Pestisida Untuk Pertanian dan Kehutanan. Komisi Pestisida Departemen Pertanian, Jakarta.
  3. Frank C. Lu. 1994. Toksikologi Dasar. Universitas Indonesia Jakarta.
  4. Gritter, R. J., J. M. Bobbitt, A. E. Schwarting. 1985. Intoduction to Chromatography. Halden Day Inc Oakland, USA. Diterjemahan oleh K.
  5. Padmawinata.1991. Pengantar Kromatografi. ITB Bandung.
  6. Habiba, R., A, H. M. Ali and S. M. Ismail. 1992. Biochemical Effect of Profenofos Residues in Potatoes, J. Agric. Food Chem, 40, 1852- 1855.
  7. Heberle, S. A., D. S. Aga, R. Hany and S.R. Muller. 2000. Simultaneus Quantification of Acetocnilide Herbicide and their Oxanilic and Sulfonic Acid Metabolite in Natural Waters. Anal. Chem. 72 : 840-845
  8. Ishii, D. 1988. Introduction to Microscale High - Performance Liquid Chromatography, VCH Publishers Inc, New York.
  9. Ishii, Y., I. Kobori, Y. Araki, S. Kurogochi, K Iwaya and S. Kagabu, 1994. HPLC Determination of The New Insecticide Imidacloprid and Its Behavior in Rice and Cucumber. J. Agric. Food Chem. 42, 2917 – 2921
  10. Pavan, F.A., R.M. Dallago, R.Zanella and A.F. Martins. 1999. Determination of Deltamethrin in Cattle Dipping Baths by High Performance Liquid Chromatography. J. Agric. Food Chem. 47 : 174 – 176 Peresz,
  11. M., J. Alario, A. Varquez and J. Villeer. 2000. Pesticide Residue Analysis by off-line SPE and on–line Reverser phase LC-GC Using The Thought Oven Transfer Adsorpstion/Desorption Interface. Anal. Chem. 72 : 846-852.
  12. Pichon, V, L. Chen., and M.C. Hennion. 1995. On-line Preconsentrration and Liquid Chromatographyc Analysis of Phenylurea Pesticides in Environmental Water Using A Silica-Based Immunosorbent. Anal. Chem. Acta. 311, 429-436.
  13. Poole, C.F. and S.K. Poole. 1994. 1st ed. Chromatography Today. Elsevier Science B.V. Netherlands. pp. 545-550.
  14. Riza, V.T. dan Gayatri. 1994. Ingatlah Bahaya Pestisida. Bunga Rampai Residu Pestisida dan Alternatifnya. Pesticide Action Network (PAN), Jakarta.
  15. Sherwa, J. 1993. Pesticide. Anal. Chem. 65: 40R- 54R
  16. Skoog, D. A. 1985. Principles of Instrumental Analysis. 3rded. Saunders Golden Sumburst Series, New York.
  17. Weiss, J. 1995. Ion Chromatography. 2nded. VCH Publisher Inc, New York.