Main Article Content

Abstract

[DETECTION OF TUNGRO VIRUS ON RICE IN BENGKULU]. Several types of viruses have been reported to infect rice plants in Indonesia, including tungro, dwarf grass, and empty dwarfs. The spread of tungro in Indonesia in the beginning was only limited to certain regions in South Sulawesi, South Kalimantan, West Nusa Tenggara and North Sulawesi, but later it expanded to East Java, Central Java and Yogyakarta. In this study a tungro disease survey was conducted in several areas in Bengkulu Province. Virus detection is done by Loop-medated isothermal amplification (LAMP). Observations in the field showed that there were typical symptoms of tungro infection in rice plants, namely orange leaves starting from leaves to 2 and 3, little tillers, stunted plant growth. The results of detection with LampPCR showed rice samples from the district Pondok Kelapa Kab Bengkulu Tengah is positively infected with tungro virus.

Article Details

How to Cite
Sutrawati, M., Sariasih, Y., Priyatiningsih, P., & Ladja, F. T. (2019). DETEKSI VIRUS TUNGRO PADA PADI DI BENGKULU. Jurnal Ilmu-Ilmu Pertanian Indonesia, 21(2), 99–102. https://doi.org/10.31186/jipi.21.2.99-102

References

  1. Agrios, G.N. (2012). Plant Pathology. Academic Press., San Diego.
  2. Azzam, O., Chancellor, T.C.B. (2002). The biology, epidemiology, and management of rice tungro disease in Asia. Plant Disease, 86(2), 88-100.
  3. Boubourakas, I.N., Fukuta, S., Kyriakopoulou, P.E.(2009). Sensitive and rapid detection of peach latent mosaic viroid by the reverse transcription loop-mediated isothermal amplification. J. Virol. Methods 160, 63–68. DOI : https://doi.org/10.1016/j.jviromet.2009.04.021
  4. Burhanuddin, Widiarta, I.N., Hassanuddin, A. (2006). Penyempurnaan pengendalian terpadu penyakit tungro dengan strategi menghindari infeksi dan pergiliran varietas tahan. JHPT Tropika, 6(2), 92-99. DOI : http://dx.doi.org/10.23960/j.hptt.2692-99.
  5. Daradjat, A. A., Widiarta, I.N. & Hasanuddin, A. (1999). Breeding for rice tungro virus resistance in Indonesia. In T. C. B. Chancellor, O. Azzam,
  6. Kong Luen Heong (Ed). Rice Tungro Disease Management (pp. 31-38). IRRI, Philippines.
  7. Fukuta, S., Iida, T., Mizukami, Y., Ishida, A., Ueda, J., Kanbe, M., Ishimoto, Y. (2003). Detection of Japanese yam mosaic virus by RT-LAMP. Arch. Virol, 148,1713–1720. DOI: https://doi.org/10.1007/s00705-003-0134-5
  8. Fukuta, S., Ohishi, K., Yoshida, K., Mizukami, Y., Ishida, A., Kanbe, M. (2004). Development of immunocapture reverse transcription loop-mediated isothermal amplification for the detection of tomato spotted wilt virus from chrysanthemum. J. Virol. Methods, 121, 49–55. DOI: https://doi.org/10.1016/j.jviromet.2004.05.016
  9. Hibino, H., Roechan, M., Sudarisman, S.(1978). Association of two types of virus particles with penyakit habang (tungro disease) of rice in Indonesia. Phytopathology, 68,1412-1416. DOI: https://doi.org/10.1094/Phyto-68-1412
  10. Hibino, H. Saleh, N., Roechan, M. (1979). Transmission of two kinds of rice tungro-associated viruses by insect vectors. Phytopathology, 69,1266-1268.
  11. Loebenstein, G. & Gera, A. (1993). The local lesion response to viruses possibilities for engineering resistant plants. Biotechnology in plant disease control. Wiley-liss, Inc. p.105-113.
  12. Notomi, T., Okayama, H., Masubuchi, H., Yonekawa, T., Watanabe, K., Amino, N., Hase,T., (2000). Loop-mediated isothermal amplification of DNA. Nucl. Acids Res. 28, e63.
  13. Sama, S. (1985). Penerapan konsep pergiliran varietas dalam pengelolaan penyakit tungro.Makalah Temu Lapang Pengendalian Penyakit Tungro. Banyumas, 18-19 September 1985.
  14. Varga, A., James, D., (2006). Use of reverse transcription loop-mediated isothermal amplification for the detection of Plum pox virus. J. Virol. Methods, 138, 184–190. DOI: https://doi.org/10.1016/j.jviromet.2006.08.014