Main Article Content
Abstract
This study aimed to investigate research about cytotoxicity correlation level of Phaleria macrocarpa (Scheff) Boerl also known as Mahkota dewa leaves methanol extract against MCF-7 cell in vitroto investigate the relationship between them in the goal to investigate alternative low cost herbal medicine agents to fight breast cancer. Cytotoxicity properties of samples against MCF-7 breast cancer cell lines was performed by using the Microtetrazolium (MTT) assay against MCF-7 cell line. The correlation between concentration of crude and cytotoxic activity was interpreted by statistical analyses. The study showed that P. macrocarpa leaves extracts showed cytoxicity activity against breast cancer MCF-7 cell lines which IC50 concentration showed at 15 µg/mL Correlation between concentration of extract and cytotoxicity property (absorbance value) were founded in weak relationship (R = 0.372, R2 = 0.138). It could be effect of many different compounds in the P. macrocarpa leaves methanol extracts may cause the pharmacological interactions, so lower or higher concentration will be antagonistic effect on absorbance or cell viability. Further study on its mechanism pathway on revealing against breast cancer could be explored. Furthermore, the natural product derived from P. macrocarpa leaves methanol extracts have potential use as alternative drugs against breast cancer.
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References
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- Rasjidi, Imam, 2009, Deteksi Dini & Pence-gahan Kanker pada Wanita .Cetakan 1 , PT Sagung Seto, Jakarta , ISBN9789793288956.
- Andriani. Y, Mohamad, H. , 2013, Potential Therapeutic Lead Coumpouds From Our Local Coastal Forest,, The 26th Symposium of Malaysia Analytical Sciences (SKAM 26) , Khuching Serawak Malaysia, 4-5 December .
- Eldeen, I M.S Mohamed, H , Tan, WN.Fong. J Y S, Andriani Y and Muhammad TST, April 2016, Cyclooxygenase, 5-Lipoxy genase and Acetylcholinesterase Inhibitory Effects of Fractions Containing, ?-Guaiene and Oil Isola-ted from the Root of Xylocarpus moluccensis, Research Journal of Medicinal Plant . http://www. scialert.net.
- Andriani Y. 2005, Pengaruh Ekstrak Daun Jati Belanda (Guazuma ulmifolia Lamk.) Terhadap Bobot Badan Kelinci Yang Diberi Pakan Berlemak, Jurnal Gradien, 1(2).
- Mohamad H , Andriani Y , Bakar K, Siang CC, Syamsumir, DF Alias A and Radzi SAM, 2015, Effect of drying method on anti-microbial, anti-oxidant activities and isolation of bioactive compounds from Peperomia pellucida (L) Hbk, Journal of Chemical and Pharmaceutical Research, 7(8). http:// www.jocpr.com.
- Andriani Y. 2008, Toksisitas Fraksi Aktif Steroid Ekstrak Daun Jati Belanda (Guazuma ulmifolia Lamk.) Terhadap Aktivitas Serum Glutamat Oksalat Transaminase (SGOT) Dan Serum Glutamat Piruvat Transaminase (SGPT) Pada Tikus Putih Jurnal Gradien, 4(2).
- Andriani. Y, 2016, Hibiscus tiliaceus Leaves as Antioxidant and Anti – Inflammantory Potential Agents, International conference on Natural Products ( ICNP), Surabaya Indonesia , 24-26 Agustus.
- Andriani,Y , M N Ramli, M N Syamsumir, DF Kassim, MNI Jaafar, J Aziz, NA Marlina, L Musa, NS, Mohamad H, 2015, Phytochemical Analysis, Antioxidant, Anti bacterial and Cy-totoxicity Properties Of Keys and Cores Parts Of Pandanus tectorius Fruits, Arabian Journal of Chemistry, http://www.journal. elsevier.com.
- Andriani. Y, 2016, Anti bacterial, cytotoxicity and anti-inflammatory activities of Hexane frac-tions from Pandanus Tectorius keys part, Inter-national conference on Natural Products (ICNP) , Surabaya Indonesia , 24-26 Agustus .
- Andriani. Y, 2017, Anti Atherosclerosis and Anti–Hypercholesterolemia Potencies of Pandanus tectorius Fruits Through Increasing the SR-B1 Expression in Vitro , International conference on Natural Products ( ICNP), Swiss Garden Hotel Lumut Perak Malaysia, 14-16 March .
- Andriani. Y, 2016, Anti bacterial and anti bio-film potencies of mangrove –associated Hibis-cus tiliaceus, International conference on Na-tural Products (ICNP), Permai Inn Hotel Kuala Terengganu Malaysia, 12-14 March.
- Radzi, SAM Andriani Y, Mohamad H ., Muhammad TST, Saidin J, 2015, In-Vitro Anti -Inflammatory Activities Of Extracts From Bacteria Associated With Marine Sponges : Theonella SP. Jurnal Teknologi (Sciences & Engineering), 77(25).
- Zalilawati, M. R Andriani, Y Shaari, K , Bourgougnon, N Ali, AM , Muhammad TST and Mohamad H , 2015, Induction of apoptosis and anti HSV-1 activity of 3-(phenethylamino) demethyl(oxy) aaptamine from a Malaysian Aaptos aaptos, Journal of Chemical and Pharmaceutical Research, 7(10).
- Andriani, Y, Syamsumir, DF Yee, TC, Harisson, FS Herng, GM Abdullah SA Oroscoa, CA Orosco, CA, Ali, AM Latip, J , Kikuzaki, H .Mohamad H, 2016, Biological Activities of Isolated Compounds from Three Edible Malay-sian Red Seaweeds, Gracilaria changii, G. Manilaensis and Gracilaria sp, Natural Product Communications , 11(8).
- Andriani Y, 2007, Uji Aktivitas Antioksidan Ekstrak Betaglukan Dari Saccharomyces cerevisiae , Jurnal Gradien , 3(1).
- Andriani Y, 2014, Anti –Inflammatory Potential of Methanol Extract From Haliclona sp- Associated Bacteria on LPS –stimulated RAW 264.7, Symposium of Malaysian Society for Microbiology (MSM) , Terengganu Equestrian Resort (TER) Kuala Terengganu Malaysia, 6-8 December .
- Andriani Y, 2014 , The Miracolous Mahko-ta Dewa, Article in Voyages of Discovery UMT, 2:9,Publisher : University Malaysia Terengganu, Kuala Terengganu.
- Andriani Y, Wahid MEA Muhammad TST and Mohamad H, January 2011, AntibacterialL, Radical – Scavenging Activities And Cyto-toxicity Properties Of Phaleria macrocarpa (Scheff.) Boerl leaves In HEPG2 Cell Lines International Journal of Pharmaceutical Science and Research, Volume 2, Issue 7 ISSN: 0975-8232. http://www ijpsr. com.
- Andriani. Y, 2010, Study Correlation between antioxidant activity and total phenolics content of Phaleria macrocarpa leaves extract,UMTAS International conference, University Malaysia Terengganu (UMT) Kuala Terengganu Malaysia 6-8 Mei.
- Andriani Y, Chew G.S., Muhammad T.S.T, Mohamad H , Latip J., Wahid MEA, January 2014, A new 4',6-dihydroxy,4-methoxybenzo phenone-2-O-?-D-gentiobioside and 4',6- di hydroxy, 4 methoxy benzo phenone-2-O-?-D-glucoside isolated from methanolic extract of Phaleria macrocarpa (Scheff.) Boerl leaves, International Journal of Phytomedicine, Volume 6, http://www. arjournals.org.
- Harahap Y, Syafhan NF, Karsono B, 2007, In Vitro cytotoxicity test of Mahkota dewa fruit dosage form on MCF-7 cell, Jurnal Bahan Alam Indonesia. 6(2). http://jbai.iregway.com/index. php/jurnal.
- Andriani Y, Muhammad TST, Mohamad H, Saidin J , Syamsumir DF, Chew GS and Wahid MEA, March 2015, Phaleria macrocarpa Boerl. (Thymelaeaceae) Leaves Increase SR-BI Expression and Reduce Cholesterol Levels in Rats Fed a High Cholesterol Diet , Molecules , Volume 20, http://www.mdpi.com.
- Sajuthi, Dondin., 2001, Ekstraksi, Fraksinasi, Karakterisasi, dan Uji Hayati In Vitro Senyawa Bioaktif Daun Dewa (Gynura pseudochina L.) sebagai Antikanker Tahap II, Buletin Kimia, jilid 1, http://www. journal.ipb.ac.id.
- Mosmann, T, 1983, Rapid colorimetric assay for cellular growth and survival: application to pro-liferation and cytotoxicity assays, Journal of Immunology Methods, Volume 65 Issues 1–2, https:// www.journals .elsevier.com.
- Andriani. Y, 2015, Apotosis property of Hypnophytum formicarum against MCF-7 Cells International conference on Natural Products ( ICNP), Perak Malaysia, 24-25 March .
- Andriani. Y, ,2016, Study on Cytotoxicity Activity of Hibiscus tiliaceus Against Breast Cancer Cells (MCF-7), International Conferen-ce on Natural Products ( ICNP), University Teknologi Malaysia (UTM) Johor Baru Malaysia , 10-11 November.
- Golden, E. B., Lam, P.Y., Kardosh,A.,Gaffney, K.J, Cadenas,E., Louie,S. G., Petasis, N.A., Chen, T. C., Schonthal., A. H. 2009. Green tea polyphenols block the anticancer effects of bortezomib and other boronic acid–based proteasome inhibitors. Blood Journal, Volume 113 Issue 23, http://www.bloodjornal.org.
References
Houghton , A.R dan Chamberlain's , D.G ,2012 ; Gejala dan Tanda dalam Kedokteran Klinis: Pengantar Diagnosis Medis, Edisi 13 , PT Indeks , Jakarta, ISBN: 979-062-283-X.
Rasjidi, Imam, 2009, Deteksi Dini & Pence-gahan Kanker pada Wanita .Cetakan 1 , PT Sagung Seto, Jakarta , ISBN9789793288956.
Andriani. Y, Mohamad, H. , 2013, Potential Therapeutic Lead Coumpouds From Our Local Coastal Forest,, The 26th Symposium of Malaysia Analytical Sciences (SKAM 26) , Khuching Serawak Malaysia, 4-5 December .
Eldeen, I M.S Mohamed, H , Tan, WN.Fong. J Y S, Andriani Y and Muhammad TST, April 2016, Cyclooxygenase, 5-Lipoxy genase and Acetylcholinesterase Inhibitory Effects of Fractions Containing, ?-Guaiene and Oil Isola-ted from the Root of Xylocarpus moluccensis, Research Journal of Medicinal Plant . http://www. scialert.net.
Andriani Y. 2005, Pengaruh Ekstrak Daun Jati Belanda (Guazuma ulmifolia Lamk.) Terhadap Bobot Badan Kelinci Yang Diberi Pakan Berlemak, Jurnal Gradien, 1(2).
Mohamad H , Andriani Y , Bakar K, Siang CC, Syamsumir, DF Alias A and Radzi SAM, 2015, Effect of drying method on anti-microbial, anti-oxidant activities and isolation of bioactive compounds from Peperomia pellucida (L) Hbk, Journal of Chemical and Pharmaceutical Research, 7(8). http:// www.jocpr.com.
Andriani Y. 2008, Toksisitas Fraksi Aktif Steroid Ekstrak Daun Jati Belanda (Guazuma ulmifolia Lamk.) Terhadap Aktivitas Serum Glutamat Oksalat Transaminase (SGOT) Dan Serum Glutamat Piruvat Transaminase (SGPT) Pada Tikus Putih Jurnal Gradien, 4(2).
Andriani. Y, 2016, Hibiscus tiliaceus Leaves as Antioxidant and Anti – Inflammantory Potential Agents, International conference on Natural Products ( ICNP), Surabaya Indonesia , 24-26 Agustus.
Andriani,Y , M N Ramli, M N Syamsumir, DF Kassim, MNI Jaafar, J Aziz, NA Marlina, L Musa, NS, Mohamad H, 2015, Phytochemical Analysis, Antioxidant, Anti bacterial and Cy-totoxicity Properties Of Keys and Cores Parts Of Pandanus tectorius Fruits, Arabian Journal of Chemistry, http://www.journal. elsevier.com.
Andriani. Y, 2016, Anti bacterial, cytotoxicity and anti-inflammatory activities of Hexane frac-tions from Pandanus Tectorius keys part, Inter-national conference on Natural Products (ICNP) , Surabaya Indonesia , 24-26 Agustus .
Andriani. Y, 2017, Anti Atherosclerosis and Anti–Hypercholesterolemia Potencies of Pandanus tectorius Fruits Through Increasing the SR-B1 Expression in Vitro , International conference on Natural Products ( ICNP), Swiss Garden Hotel Lumut Perak Malaysia, 14-16 March .
Andriani. Y, 2016, Anti bacterial and anti bio-film potencies of mangrove –associated Hibis-cus tiliaceus, International conference on Na-tural Products (ICNP), Permai Inn Hotel Kuala Terengganu Malaysia, 12-14 March.
Radzi, SAM Andriani Y, Mohamad H ., Muhammad TST, Saidin J, 2015, In-Vitro Anti -Inflammatory Activities Of Extracts From Bacteria Associated With Marine Sponges : Theonella SP. Jurnal Teknologi (Sciences & Engineering), 77(25).
Zalilawati, M. R Andriani, Y Shaari, K , Bourgougnon, N Ali, AM , Muhammad TST and Mohamad H , 2015, Induction of apoptosis and anti HSV-1 activity of 3-(phenethylamino) demethyl(oxy) aaptamine from a Malaysian Aaptos aaptos, Journal of Chemical and Pharmaceutical Research, 7(10).
Andriani, Y, Syamsumir, DF Yee, TC, Harisson, FS Herng, GM Abdullah SA Oroscoa, CA Orosco, CA, Ali, AM Latip, J , Kikuzaki, H .Mohamad H, 2016, Biological Activities of Isolated Compounds from Three Edible Malay-sian Red Seaweeds, Gracilaria changii, G. Manilaensis and Gracilaria sp, Natural Product Communications , 11(8).
Andriani Y, 2007, Uji Aktivitas Antioksidan Ekstrak Betaglukan Dari Saccharomyces cerevisiae , Jurnal Gradien , 3(1).
Andriani Y, 2014, Anti –Inflammatory Potential of Methanol Extract From Haliclona sp- Associated Bacteria on LPS –stimulated RAW 264.7, Symposium of Malaysian Society for Microbiology (MSM) , Terengganu Equestrian Resort (TER) Kuala Terengganu Malaysia, 6-8 December .
Andriani Y, 2014 , The Miracolous Mahko-ta Dewa, Article in Voyages of Discovery UMT, 2:9,Publisher : University Malaysia Terengganu, Kuala Terengganu.
Andriani Y, Wahid MEA Muhammad TST and Mohamad H, January 2011, AntibacterialL, Radical – Scavenging Activities And Cyto-toxicity Properties Of Phaleria macrocarpa (Scheff.) Boerl leaves In HEPG2 Cell Lines International Journal of Pharmaceutical Science and Research, Volume 2, Issue 7 ISSN: 0975-8232. http://www ijpsr. com.
Andriani. Y, 2010, Study Correlation between antioxidant activity and total phenolics content of Phaleria macrocarpa leaves extract,UMTAS International conference, University Malaysia Terengganu (UMT) Kuala Terengganu Malaysia 6-8 Mei.
Andriani Y, Chew G.S., Muhammad T.S.T, Mohamad H , Latip J., Wahid MEA, January 2014, A new 4',6-dihydroxy,4-methoxybenzo phenone-2-O-?-D-gentiobioside and 4',6- di hydroxy, 4 methoxy benzo phenone-2-O-?-D-glucoside isolated from methanolic extract of Phaleria macrocarpa (Scheff.) Boerl leaves, International Journal of Phytomedicine, Volume 6, http://www. arjournals.org.
Harahap Y, Syafhan NF, Karsono B, 2007, In Vitro cytotoxicity test of Mahkota dewa fruit dosage form on MCF-7 cell, Jurnal Bahan Alam Indonesia. 6(2). http://jbai.iregway.com/index. php/jurnal.
Andriani Y, Muhammad TST, Mohamad H, Saidin J , Syamsumir DF, Chew GS and Wahid MEA, March 2015, Phaleria macrocarpa Boerl. (Thymelaeaceae) Leaves Increase SR-BI Expression and Reduce Cholesterol Levels in Rats Fed a High Cholesterol Diet , Molecules , Volume 20, http://www.mdpi.com.
Sajuthi, Dondin., 2001, Ekstraksi, Fraksinasi, Karakterisasi, dan Uji Hayati In Vitro Senyawa Bioaktif Daun Dewa (Gynura pseudochina L.) sebagai Antikanker Tahap II, Buletin Kimia, jilid 1, http://www. journal.ipb.ac.id.
Mosmann, T, 1983, Rapid colorimetric assay for cellular growth and survival: application to pro-liferation and cytotoxicity assays, Journal of Immunology Methods, Volume 65 Issues 1–2, https:// www.journals .elsevier.com.
Andriani. Y, 2015, Apotosis property of Hypnophytum formicarum against MCF-7 Cells International conference on Natural Products ( ICNP), Perak Malaysia, 24-25 March .
Andriani. Y, ,2016, Study on Cytotoxicity Activity of Hibiscus tiliaceus Against Breast Cancer Cells (MCF-7), International Conferen-ce on Natural Products ( ICNP), University Teknologi Malaysia (UTM) Johor Baru Malaysia , 10-11 November.
Golden, E. B., Lam, P.Y., Kardosh,A.,Gaffney, K.J, Cadenas,E., Louie,S. G., Petasis, N.A., Chen, T. C., Schonthal., A. H. 2009. Green tea polyphenols block the anticancer effects of bortezomib and other boronic acid–based proteasome inhibitors. Blood Journal, Volume 113 Issue 23, http://www.bloodjornal.org.