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Abstract
Bakteri selulolitik memiliki kemampuan degradasi selulosa dan membuat karbohidrat lebih mudah dicerna bagi ternak. Penelitian ini bertujuan untuk mengidentifikasi bakteri selulolitik di tanah, kayu lapuk dan daun dari mangrove pulau Bangka melalui skrining dan analisis gen 16s rRNA. Penelitian dilakukan dari September 2017 hingga Maret 2018. Isolat TSL7 dan TSS4 dari Mangrove Tukak Sadai dan SLS5 dari Mangrove Sungailiat yang memiliki kemampuan mendegradasi selulosa terbesar berdasarkan hasil skrining menjadi isolat yang diidentifikasi pada gen 16S rRNA untuk diurutkan dan analisis BLAST. Analisis gen bank menunjukkan kedekatan dengan Pseudomonas aeruginosa dengan query cover 37% - 87%. Hasil query cover rendah dihasilkan karena sekuens yang sesuai pendek. Kontaminasi dan perubahan komunitas karena seleksi lingkungan dengan adanya logam berat dapat menjadi penyebab dominasi Pseudomonas aeruginosa. Pseudomonas aeruginosa yang bersifat patogen tidak disarankan untuk diaplikasikan sebagai pendegradasi selulosa pakan ternak.
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References
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References
Ahmad Fasmi, 2013. Distribusi Dan Prediksi Tingkat Pencemaran Logam Berat (Pb, Cd, Cu, Zn, Dan Ni) Dalam Sedimen Di Perairan Pulau Bangka Menggunakan Indeks Beban Pencemaran Dan Indeks Geoakumulasi. Jurnal Ilmu dan Teknologi Kelautan Tropis, Vol. 5, No. 1.
Chantarasiri A. 2015. Aquatic Bacillus cereus JD0404 isolated from the muddy sediments of mangrove swamps in Thailand and characterization of its cellulolytic activity. Egypt J Aquat Res. 41(3).
Deepika K.V., P.Ramu Sridharb, P.V Bramhacharia. 2015. Characterization and antifungal properties of rhamnolipids produced by mangrove sediment bacterium Pseudomonas aeruginosa strain KVD-HM52. Biocatalysis and Agricultural Biotechnology. Vol 4 No. 4.
Kurniawan et al, 2018b. Isolation and Identification of cellulolytic bacteria from mangrove sediment in Bangka Island. IOP Conf. Series: Earth and Environmental Science 137.
Kurniawan et al, 2018c. Isolasi Dan Identifikasi Bakteri Pendegradasi Selulosa Asal Ekosistem Mangrove Tukak Sadai, Bangka Selatan. Jurnal Perikanan Pantura. Volume 1, Nomor 2
Kurniawan, A., Prihanto, A.A., Puspita, S., Kurniawan, A., Asriani, E., Sambah, A.B. 2018a. Cellulolytic Bacteria Mangrove Leaf Litter In Bangka Island. Samakia: Jurnal Ilmu Perikanan, 9 (1) : 06-11
Lubis Y.P, Yunasfi, R. Leidonald, 2013. Jenis-Jenis Bakteri Pada Luka Ikan Patin (Pangasius djambal). Aquacoastmarine. Universitas Sumatera Utara, Medan.
Maulani SH. 2014. Isolation And Identification Of Cellulolitic Bacteria From Land Of Estuary River Mangrove Gunung Anyar, Surabaya. ADLN Perpustakaan Universitas Airlangga.
Nurrochman F. 2015. Exploration of Cellulolytic Bacteria From Land Mangrove Forest Baros Kretek, Bantul, Yogyakarta. Publication manuscript. Faculty of Teacher Training and Education. Muhammadiyah Surakarta University.
Putri. R.W, W. Tjahjaningsih, D. Handijatno. 2008. Daya Antibakterial Pigmen Pyocyanin Dari Isolat Pseudomonas aeruginosa Terhadap Aeromonas hydrophila Secara In Vitro. Berkala Ilmiah Perikanan Vol. 3 No. 1.
Rigonato J, Kent AD, Gumiere T, Branco LHZ, Andreote FD, Fiore MF. 2017. Temporal assessment of microbial communities in soils of two contrasting mangroves. Brazilian J Microbiol
Strohl WA, Rouse H, Fisher BD. 2001. Microbiology. USA: Lippincott Williams & Wilikns.
Sari P.S, D. Rosalina, W. Adi, 2017. Bioakumulasi timbal (Pb) dan cadmium (Cd) pada lamun Cymodocea serrulata di Perairan Bangka Selatan. Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan. Volume 6, Number 2.
Sari P.S. dan D. Rosalina, 2014. Tingkat Keberhasilan Penanaman Mangrove pada Lahan Pasca Penambangan Timah di Kabupaten Bangka Selatan. Maspari Journal, Vol. 6, No. 2.
Wahyuni. H, S.B Sasongko, D. P. Sasongko, 2013. Kandungan Logam Berat pada Air, Sedimen dan Plankton di Daerah Penambangan Masyarakat Desa Batu Belubang Kabupaten Bangka Tengah. Prosiding Seminar Nasional Pengelolaan Sumberdaya Alam dan Lingkungan.