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September 19, 2023
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December 31, 2022
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Abstract

Latar Belakang: Akumulasi asprin menghasilkan senyawa reaktif berupa radikal bebas yang menginduksi terjadinya stres oksidatif sehingga akan mepengaruhi fungsi ginjal. Ekstrak etanol daun C. blumei Benth mengandung senyawa metabolit sekunder golongan alkaloid, flavonoid, saponin, fenol, antrakuinon, tanin, dan steroid yang memiliki kemampuan mencegah pembentukan radikal bebas, antiinflamasi dan memperbaiki fungsi ginjal. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak etanol daun ati-ati (C. blumei Benth) terhadap kadar ureum dan kreatinin darah mencit (M. musculus) yang diinduksi aspirin.


Metode: Penelitian ini bersifat eksperimental post test only control group design. Sampel penelitian menggunakan 30 ekor mencit (M. musculus) yang dibagi menjadi 6 kelompok, yaitu kelompok kontrol (P0), perlakuan I (P1) yang diberi aspirin 84 mg/kgBB, perlakuan II (P2) diberi aspirin 84 mg/kgBB + silimarin 100 mg/kgBB, perlakuan III (P3) aspirin 84 mg/kgBB + ekstrak etanol daun C. blumei Benth 500 mg/kgBB, perlakuan IV (P4) aspirin 84 mg/kgBB + ekstrak etanol daun C. blumei Benth 1000 mg/kgBB dan perlakuan V (P5) aspirin 84 mg/kgBB + ekstrak etanol daun C. blumei Benth 2000 mg/kgBB. Seluruh kelompok mendapat perlakuan selama 11 hari yang terdiri dari 4 induksi aspirin dan 7 hari pemberian silimarin atau ekstrak. Pada hari ke-12 mencit dilakukan pengambilan darah secara intracardiac untuk dilakukan pemeriksaan kadar ureum dan kreatinin darah. Data analisis dilakukan dengan uji one-way ANOVA dan uji post-hoc Tukey.


Hasil: Hasil uji one-way ANOVA menunjukan terdapat perbedaan kadar ureum dan kreatinin darah yang signifikan antar kelompok dengan nilai p yaitu 0,020 dan 0,003. Kelompok P5 mengalami penurunan kadar ureum dan kreatinin yang signifikan dengan nilai p 0,024 dan 0,034, meskipun kelompok P5 tidak sebaik kelompok P0.


Kesimpulan: Pemberian ekstrak etanol daun C. blumei Benth dosis bertingkat, yaitu 2000 mg/kgBB mampu menurun kadar ureum dan kreatinin darah mencit yang induksi aspirin secara signifikan.


 


Kata kunci:  Aspirin, C. blumei Benth, silymarin, ureum, kreatinin.

Keywords

Aspirin C. blumei Benth silymarin ureum kreatinin

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Copyright (c) 2022 Indri Wahyuni, Besly Sinuhaji, Novriantika Lestari

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How to Cite
Wahyuni, I., Sinuhaji, B., & Lestari, N. (2022). Pengaruh Ekstrak Etanol Daun Ati-ati (Coleus blumei Benth) Terhadap Kadar Ureum dan Kreatinin Darah Mencit (Mus musculus) yang Diinduksi Aspirin. Jurnal Kedokteran Raflesia, 8(2), 56–65. https://doi.org/10.33369/juke.v8i2.30114
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References

  1. Saifudin A. Senyawa Alam Metabolit Sekunder: Teori, Konsep, dan Teknik Pemurnian Edisi ke-1. Yogyakarta: Deepublish; 2014.
  2. Dalimartha S. Atlas Tumbuhan Obat Indonesia Edisi ke-3. Jakarta: Trubus Agriwidya; 2006.
  3. Bauer N, Vuković R, Likić S, Jelaska S. Potential of Different Coleus blumei Tissues for Rosmarinic Acid Production. Food Technol Biotechnol. 2015;53(1):3–10.
  4. Marianny RS. Aktivitas Anthelmintika Ekstrak Daun Miana (Coleus blumei) Terhadap Hymenolepis microstoma In Vitro. Skripsi. Institut Pertanian Bogor. 2008
  5. Pakadang SR, Wahjuni CU, Notobroto HB, Winarni D, Dwiyanti R, Yadi, et al,. Immunomodulator Potential of Miana Leaves (Coleus scutellarioides (L) Benth) in Prevention of Tuberculosis Infection. Am J Microbiol Res. 2015;3(4):129–34.
  6. Wakhidah AZ, Silalahi M. Etnofarmakologi Tumbuhan Miana (Coleus scutellariodes (L.) Benth) pada Masyarakat Halmahera Barat, Maluku Utara. J Pro-Life. 2018;5(2):567–78.
  7. Suva MA, Patel AM, Sharma N. Coleus Species :Solenostemon scutellarioides. Inven Journals Planta Act. 2015;(2):1–5.
  8. Moron MJ, Acero LH. Mayana (Coleus blumei) Leaves Ointment in Wound Healing of Albino Rats (Rattus albus). Int J Food Eng. 2017;3(1):18–22.
  9. Saraswati, Katnoria JK, Nagpal AK. Analytical Techniques for Phytochemicals Screening and Bioactivities Of Some Coleus Species : A Review. J Pharm Sci Res. 2016;8(4):227–37.
  10. Pisoschi AM, Pop A. The role of antioxidants in the chemistry of oxidative stress. Eur J Med Chem [Internet]. 2015;97(1):55–74. Available from: http://dx.doi.org/10.1016/j.ejmech.2015.04.040
  11. Dennis JM, Witting PK. Review: Protective Role for Antioxidants in Acute Kidney Disease. Nutrients. 2017;9(718):1–25.
  12. Patel SS, Savjani JK. Systematic review of plant steroids as potential anti- inflammatory agents : Current status and future perspectives. J Phytopharm. 2015;4(2):121–5.
  13. Zhou Y, Boudreau DM, Freedman AN. Trends in the use of aspirin and nonsteroidal anti-inflammatory drugs in the general U.S. population. Pharmacoepidemiol Drug Saf. 2014;23:43–50.
  14. PERKI. Panduan praktik klinis (ppk) dan clinical pathway (cp) penyakit jantung dan pembuluh darah. Jakarta: Perhimpunan Dokter Spesialis Kardiovaskular Indonesia (Indonesian Heart Association); 2016. p. 1–378.
  15. Perazella MA. Pharmacology behind Common Drug Nephrotoxicities. Clin J Am Soc Nephrol. 2018;14:1–12.
  16. Gummin DD, Mowry JB, Spyker DA, Brooks DE, Fraser MO, Banner W. 2016 Annual Report of the American Association of Poison Control Centers National Poison Data System (NPDS): 34th Annual Report. Cilinical Toxicol. 2017;55(10):1072–254.
  17. Bouzenna H, Dhibi S, Samout N, Rjeibi I, Talarmin H, Elfeki A, et al., The protective effect of Citrus limon essential oil on hepatotoxicity and nephrotoxicity induced by aspirin in rats. Biomed Pharmacother. 2016;83:1327–34.
  18. Sherwood L. Fisiologi Manusia: Dari Sel ke Sistem. 8th ed. Jakarta: Buku Kedokteran EGC; 2015.
  19. Shahbazi F, Dashti-khavidaki S, Khalili H, Lessan-pezeshki M. Potential Renoprotective Effects of Silymarin Against Nephrotoxic Drugs : A Review of Literature Potential. J Pharm Pharm Sci. 2012;15(1):112–23.
  20. Bektur NE, Sahin E, Baycu C, Unver G. Protective effects of silymarin against acetaminophen-induced hepatotoxicity and nephrotoxicity in mice. Toxicol Ind Health. 2013;32(4):1–12.
  21. Nuraeni D. Pengaruh Pemberian Aspirin Dosis Toksik Per Oral terhadap Gambaran Histopatologi Gaster, Duodenum, dan Jejunum Tikus Wistar. Skripsi. Fakultas Kedokteran Universitas Diponegoro: Semarang. 2007.
  22. Nissa C, Madjid IJ. Potensi Glukomanan pada Tepung Porang sebagai Agen Ati-obesitas pada Tikus dengan Induksi Diet Tinggi Lemak. J Klin Indones. 2016;13(1).
  23. Anggraini D. Pengaruh Ekstrak Etanol Coleus blumei Terhadap Gambaran Histopatologi Gaster Mencit (Mus musculus) yang Diinduksi Aspirin. Skripsi. Fakultas Kedokteran dan Ilmu Kesehatan Universitas Bengkulu. 2019.
  24. Awdishu L, Mehta RL. The 6R’s of drug induced nephrotoxicity. BMC Nephrol. BioMed Central. 2017;18(124):1–12.
  25. Bhattcharyya S, Ghosh S, Sil CP. Amelioration of aspirin Induced Oxidative Impairment and Apoptotic Cell Death by a Novel Antioxidant Protein Molecule Isolated from the Herb Phyllanthus niruri. PLoS. 2014;9(1):1–14.
  26. Tsimihodimos V, Psychogios N, Kakaidi V, Bairaktari E, Elisaf M. Salicylate-induced proximal tubular dysfunction. Am J Kidney Dis. 2016;50(1):463–7.
  27. Nwanto HU, Okolie NJC, Oze G, Okafor D, Nwosu D, Ajero C. Chages in Some Biochemical Parameters of Kidney Functions in Rats Co-Administered with Chloroquine and Aspirin. Res J Med Sci. 2007;1(2):106–9.
  28. Ridwan Y, Satrija F, Darusmanb LK, Handharyani E. Efektivitas Anticestoda Ekstrak Daun Miana (Coleus blumei Bent) terhadap Cacing Hymenolepis microstoma pada Mencit. 2010;33(1):6–11.
  29. Wang LW, Liang J, Wang XD, Yuan XF, Zhao B, Yang YW. High efficient antioxidant activity of extracts from Lepidium meyenii Walp. Asian J Chem. 2012;24(10):4795–8.
  30. Wardojo MM, Sumiwi SA, Iskandar Y, Novinda D, Mustarichie R. Antioxidant activity and phytochemical screening of Plectranthus scutellarioides L . leaves ethanol and water extracts by DPPH method. Res J Pharm Biol Chem Sci. 2018;9(954):954–61.
  31. Parwata IM. Kimia Organik Bahan Alam: Flavanoid. Denpasar; 2016.
  32. Akinpelu BA, Igbeneghu OA, Awotunde AI, Iwalewa EO, Oyedapo OO. Antioxidant and antibacterial activities of saponin fractions of Erythropheleum suaveolens (Guill. and Perri.) stem bark extract. Sci Res Essays. 2014;9(18):826–33.
  33. Dhianawaty D, Ruslin. Kandungan Total Polifenol dan Aktivitas Antioksidan dari Ekstrak Metanol Akar Imperata cylindrica (L) Beauv (Alang-alang). MKB. 2015;47(1):60–4.
  34. Hangerman AE. Tannins as Antioxidants. USA: Departement of Chemistry and Biochemistry, MiamyUniversity, Oxford; 2002.
  35. Karimi G, Vahabzadeh M, Lari P, Rashedinia M, Moshiri M. “ Silymarin ”, a Promising Pharmacological Agent for Treatment of Diseases. Iran J Basic Med Sci. 2011;14(4):308–17.
  36. Gopi KS, Reddy AG, Jyothi K, Kumar BA. Acetaminophen-induced Hepato- and Nephrotoxicity and Amelioration by Silymarin and Terminalia chebula in Rats. Toxicol Int. 2010;17(2):64–7.
  37. Hilmi SR, Dewan ZF, Kabir AKMN, Islam MM. Comparison of Effect of Silymarin and Ethanol Extract of Trigonella foenum- graecum on Gentamicin Induced Nephrotoxicity in Rats. J Curr Adv Med Res. 2019;6(1):23–7.