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Abstract
Usaha perikanan hiu yang menjanjikan di negara Indonesia ini menjadikan nilai produksi hiu terus meningkat dari tahun ke tahun. Menurut catatan FAO, Indonesia berada diposisi teratas yang banyak memproduksi hiu setiap tahunnya. Tingginya aktivitas penangkapan hiu berpengaruh besar terhadap populasi yang memengaruhi keseimbangan ekosistem laut, khususnyadi perairan Kepulauan Bangka Belitung. Tujuan penelitian ini yaitu untuk mengidentifikasi sirip ikan hiu secara molekuler dengan menggunakan gen mitokondria cytochrome c oxydase subunit I (COI) yang didaratkan di pesisir Kabupaten Bangka Selatan. Metode penelitian terdiri dari tiga tahap: pengumpulan sampel sirip ikan hiu, identifikasi molekuler (mtDNA gen Cytochrome Oxidase Subunit 1/COI), analisis filogenetik dan status konservasi ikan hiu. Hasil pencocokan karakter nukleotida gen COI dilakukan dengan menggunakan program BLAST yang terintegrasi pada laman GenBank dan menunjukkan bahwa kode sampel MSPUBB_HC_01 memiliki tingkat kemiripan 100% dengan spesies Chiloscyllium punctatum, MSPUBB_HK_02 dan MSPUBB_HS_03 masing-masing memiliki tingkat kemiripan 99.80% dan 99.69% dengan spesies Carcharhinus sealei.Jika ditinjau dari data IUCN Red List, spesies Chiloscyllium punctatumdan Carcharhinus sealeimasuk dalam kategori Near threatened(Hampir Terancam).
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References
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References
Camhi, M., S. Fowler, J. Musick, A. Brautigam and S. Fordham 1998. Sharks and their relatives, ecology and conservation. Occasional Paper of the IUCN Species Survival Commission No.20. IUCN, Gland, Switzerland and Cambridge, UK. 39p.
Claveire J.M., and C. Notredame. 2003. Bioinformatics for Dummies. Wiley Publishing, Indianapolis.
Dudgeon, C.L., Bennett, M.B. & Kyne, P.M. 2016. Chiloscyllium punctatum. The IUCN Red List of Threatened Species 2016: e.T41872A68616745. https://dx.doi.org/10.2305/IUCN.UK.2016-1.RLTS.T41872A68616745.en.
Eugene, H.K.W., Mahmood, S.S., and Robert, H. H. 2009. Identifying Sharks with DNA Barcodes: Assessing the Utility of a Nucleotide Diagnostic Approach. Molecular Ecology Resources (Suppl. 1), doi: 10.1111/j.1755-0998.2009.02653.x. 243–256.
Fatchiyah, Estri, L. A., Sri, W., dan Sri, R. 2011. Biologi Molekular – Prinsip Dasar Analisis. Erlangga, Jakarta. 8-48.
Felsenstein J. 1985. Confidence limits on phylogenies: An approach using the bootstrap. Evolution 39:783-791.
Kimura M. 1980. A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution 16:111-120.
Kumar S., Stecher G., Li M., Knyaz C., and Tamura K. 2018. MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms. Molecular Biology and Evolution 35:1547-1549.
Mcdonald J.H., and M. Kreitman. 1991. Adaptive protein evolution at the Adh locus in Drosophila. Nature. 351: 652 – 654.
Mount D.W. 2001. Phylogenetic prediction. In: Bioinformatic, Sequence and Genome Analysis. Cold Spring Harbor laboratory. New York Press. pp. 237 –280.
Nielsen R., and Z. Yang. 1998. Likehood models for detecting positively selected amino acid sites and application to the HIV-1 envelope gene. Genetics.148: 929 – 936.
Rahmad. 2013. Taksonomi Molekuler DNA Barcoding dan Analisis Filogenetik Ikan Hiu di Pelabuhan Perikanan Palabuhanratu Berdasarkan Marka Mitokondria. [Skripsi]. Departemen Ilmu dan Teknologi Kelautan. Fakultas Perikanan dan Ilmu Kelautan. Institut Pertanian Bogor.
Saitou N. and Nei M. 1987. The neighbor-joining method: A new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4:406-425.
Stecher G., Tamura K., and Kumar S. 2020. Molecular Evolutionary Genetics Analysis (MEGA) for macOS. Molecular Biology and Evolution (https://doi.org/10.1093/molbev/msz312).
Stevens, J.D., Bonfil, R., Dulvy, N.K., and Walker, P.A. 2000. The Effects of Fishing on Sharks, Rays and Chimaeras (Chondrichthyans), and the Implications for Marine Ecosystem. ICES Journal of Marine Science, 57:476-494.
Tamura K., G. Stecher, D. Peterson, A. Filipski, and S. Kumar. 2013. MEGA 6: Molecular Evolutionary Genetics Analysis version 6.0. [Computer software]. Molecular Biology and Evolution. 30:2725-2729.
Traffic. 2002. A CITES Priorities: Sharks and the Twelfth Meeting of the Conference of the Parties to CITES, Santiago Chile. IUCN and TRAFFIC Briefing document, page 2. (Online) Available at: http://www.traffic.org/news/SharksCoP12.
Ward R.D., Zemlak T.S., Innes B.H., Last P.R., and Hebert P.D.N. 2005. DNA barcoding Australia’s fish species. Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences.
White, W.T. (SSG Australia & Oceania Regional Workshop, March 2003). 2003. Carcharhinus sealei. The IUCN Red List of Threatened Species 2003: e.T41738A10551361. https://dx.doi.org/10.2305/IUCN.UK.2003.RLTS.T41738A10551361.en.
World Wild Life. 2018. Shark Species. https://www.worldwildlife.org/species/shark.
Zein, M.S.A. 2007. DNA barcode keragaman genetic, dan konservasi fauna Indonesia. Laboratorium genetika bidang zoology pusat peneliti biologi LIPI. 70 hal.