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Abstract
Salinitas termasuk dalam salah satu faktor yang berpengaruh terhadap mikroalga dalam mempertahankan tekanan osmotik protoplasma. Perubahan salinitas pada media kultivasinya akan memperlambat laju fotosintesis mikroalga. Maka dari itu, salinitas diduga mempengaruhi pertumbuhan, produksi biomassa dan kandungan pigmen (klorofil dan karotenoid) D. salina yang dihasilkan. Penelitian ini memiliki tujuan untuk melakukan kajian mengenai hubungan antara laju pertumbuhan, pighmen dan aktivitas antioksidan ekstrak polar mikroalga D salina pada media kultur bersalinitas yang berbeda. Penelitian dilakukan di Laboratorium Prodi Budidaya Perairan UNISNU – Jepara dengan metode eksperimen laboratoris. Variabel independent penelitian adalah perbedaan salinitas media kultur yaitu 20, 25, 30, 35 dan 40 ppt. Sedangkan variabel dependent penelitian adalah kepadatan sel, berat basah, kadar klorofil a, b, karotenoid dan aktitivas antioksidan terhadap radikal DPPH. Data antar variabel dilakukan analisis korelasi. Hasil penelitian menunjukkan bahwa dalam perbedaan salinitas, D salina lebih mengutamakan produksi pighmen klorofil a, dari pada klorofil b dan karotenoid (p < 0,05). Terdapat hubungan positif antara laju pertumbuhan terhadap produksi klorofil a, b dan karotenoid yaitu y = 128,2x - 10,10 (R2 = 0,938); y = 46,51x - 4,004 (R2 = 0,958); y = 50,93x - 3,393 (R2 = 0,955). Terdapat hubungan positif antara aktivitas antioksidan terhadap laju pertumbuhan, produksi klorofil a, b dan karotenoid yaitu y = -2683x + 460,5 (R2 = 0,927); y = -20,54x + 245,5 (R2 = 0,951); y = -56,39x + 225,3 (R2 = 0,924); dan y = -52,91x + 282,7 (R2 = 0,978). Semakin tinggi laju pertumbuhan D. salina maka produksi klorofil a, b dan karotenoid semakin tinggi. Selanjutnya hasil ektraksi tersebut memiliki aktifitas antioksidan yang semakin tinggi.
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References
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References
Abdille HMD, Singh RP, Jayaprakasha GK, Jena BS. 2004. Antioxidant activity of the extracts from Dillenia indica fruits. Journal of Food Chemistry. 90: 891-896.
Adenan, N.S., F.Md. Yusoff. and M. Shariff. 2013. Effect of Salinity and Temperature on the Growth of Diatoms and Green Algae. J.Fish.Aquat.Sci. 8(2) : 397-404.
Adenan, N.S., F.Md. Yusoff. and M. Shariff. 2013. Effect of Salinity and Temperature on the Growth of Diatoms and Green Algae. J.Fish.Aquat.Sci. 8(2) : 397-404.
Bold, H., Wyne, M. 1985. Introduction to The Algae: Structure and Reproduction. 2nded., Prentice-Hall Mc.Englewood Cliffs, New York, pp. 720.
Cifuentes, A.S., Gonzaléz M.A., Inostroza, I., and Aguilera, A. 2001. Reappraisal of The Physiological Attributes of Nine Strains of Dunaliella (Chlorophyceae): Growth and Pigment Content Across a Salinity Gradient. J. Phycol. 37: 334-344.
Erlina, A. 2007. Produksi Pakan Hidup. (Pelatihan Pembenihan Udang). Laboratorium Pakan Alami. Balai Besar Pengembengan Budidaya Air Payau, Jepara.
Fazeli M. R., Tofighi H., Samadi N., Jamalifar H., Fazeli A., 2006. Carotenoids accumulation by Dunaliella tertiolecta (Lake Urmia isolate) and Dunaliella salina (ccap 19/18 & wt) under Stress Conditions. Journal of Pharmaceutical Sciences 14(3):146-150.
Giuliano, G., Tavazza, R., Diretto, G., Beyer, P., & Taylor, M. A. 2008. Metabolic Engineering of Carotenoid Biosynthesis in Plants. Cell Press 3: 0167-7799.
Gunawan, A dan Roeswati. 2004. Tangkas Kimia. Kartika. Surabaya.
Hastuti, W. dan Djunaidah. 1991. Heavy Metal Activate Synthesis Of Peptides in Chlamydomonas reinhardtii. Plant. Physiol., 98: 127-136.
Helwig B. 2008. Antioxidants. http://www.exrx.net/nutrition/antioxidants/html. [24 Juli 2016].
Hirata H, Andarias I, dan Yamasaki S. 1981. Effect of salinity temperature on the growth of the marine phytoplankton Chlorella saccharophila. Mem. Fac. Fish. Kaghosima Univ. 30 : 257-262.
Hong Ye, Zhou Chunhong, Sun Yi, Zhang Xin, Liu Jun, Hu Qiuhui, Zeng Xiaoxiong, 2009, Antioxidant Activities Invitro of Ethanol Extract From Brown Seaweed Sargassum pallidum, Eur Food Res Technol 230:101-109.
Imron, M.A., Sudarno dan E.D. Masithah. 2016. Pengaruh Salinitas Terhadap Kandungan Lutein pada Mikroalga Botryococcus braunii. Journal of Marine and Coastal Science., 5(1).
Imron, M.A., Sudarno dan E.D. Masithah. 2016. Pengaruh Salinitas Terhadap Kandungan Lutein pada Mikroalga Botryococcus braunii. Journal of Marine and Coastal Science., 5(1).
Kontara, E.K.M., Djunaidah, I.S., Coutteau, P., Sorgeloos, P., 1998. Comparison of native, lyso and hydrogenated soybean phosphatidylcholine as phospholipid source in the diet of postlarval Penaeus japonicus Bate. Arch. Anim. Nutr., 51(1):1–19.
Pisal, S. Dipak and S. S. Lele. 2005. Carotenoid Production from Microalga, Dunaliella Salina. Indian Journal of Biotechnology, 10(4):476-483.
Purnamawati, F.S., T.R. Soeprobowati. 2013. Pertumbuhan Chlorella Vulgaris Beijerinck dalam Medium yang Mengandung Logam Berat Cd dan Pb Skala Laboratorium. Dalam: Seminar Nasional Biologi; Peran Biologi dalam Meningkatkan Produktivitas yang Menunjang Ketahanan Pangan. Semarang; pp. 104-116.
Rusyani, E., 2001, Pengaruh Dosis Zeolit yang Berbeda terhadap Pertumbuhan Isochrysis galbana Klon Tahiti Skala Laboratorium dalam Media Komersial. Skripsi. Institut Pertanian Bogor, Bogor. 53 hlm.
Soeder, C. and E. Stengel. 1974. Physico-chemical Factors Affecting Metabolism and Growth Rate. In: Algal physiology and chemistry, pp. 714-740, W. D. P. Stewart-red.). Univ. of California Press, Berkeley and Los Angeles, California.
Stengel. and Soeder, C. 1974. Physicochemical Factors Affecting Metabolism and Growth Rate. In: “Algal Physiology and Biochemistry”. (W.D.P. Stewart. Editor).Blackwell Scientific Publication. Oxford London Edinburgh Melbourne: 714-730.
Vo, Trung, and Duc Tran. 2014. Carotene and Antioxidant Capacity of Dunaliella salina Strains. World Journal of Nutrition and Health, 2 (2):21-23.