Efektivitas Limbah Bonggol Nanas (Ananas comosus) sebagai Bahan Antibakteri Indonesia
Abstract
The pineapple core has not been utilized optimally, despite containing several active components, one of which is the bromelain enzyme. Bromelain exhibits antibacterial properties. The objective of this study was to determine whether pineapple core extract (Ananas comosus (L.) Merr) is effective in inhibiting the growth of Escherichia coli bacteria. The study employed the disk diffusion test method to measure the clear zone around the blank disk vertically and horizontally using a Vernier caliper with units in millimeters (mm). The treatment was conducted three times with different concentrations, an incubation time of 1 x 24 hours, and a temperature of 37°C. The concentrations tested were 20%, 40%, 60%, and 100%, with ciprofloxacin as the positive control (+). The results of this study showed that a concentration of 20% produced an average inhibition zone of 7.8 mm, 40% produced an average inhibition zone of 18 mm, 60% produced an average inhibition zone of 21 mm, 80% produced an average inhibition zone of 22 mm, and 100% produced an average inhibition zone of 24 mm.
Full text article
References
hystrix Dc) terhadap beberapa bakteri. JCPS (Journal of Current Pharmaceutical Sciences), 2(1), 136-141.
Daely, P. J., Sarwendah, S., Laia, H. C. ., & Damardi, S. 2019. Uji Daya Hambat Anti Bakteri Air Perasan Daging Buah
Nanas (Ananas Comosus (L) Merr Var. Queen) terhadap Bakteri Eschericia Coli. Jurnal Ilmiah Universitas Batanghari
Jambi, 19(2), 239
Dewi, S. H., Lubis, M. S., Yuniarti, R., & Nasution, H. M. 2024. Skrining Fitokimia Dan Uji Aktivitas Antibakteri Ekstrak
Etanol Bonggol Nanas (Ananas comosus (L.) Merr). 4(1), 95–105.
Fatmawati, L. . 2019. Uji Efektivitas Antibakteri Ekstrak Kulit Nanas (Ananas comosus [L.] Merr.) Dan Kulit Pisang (Musa
paradisiaca L.) Terhadap Pertumbuhan Bakteri Escherichia coli. In Program Studi Biologi Fakultas Sains Dan Teknologi
Universitas Islam Negeri Sunan Ampel Surabaya.
Indah Sari, V., Putri, V. J., & Rahmah, A. 2022. Peningkatan Pengetahuan Melalui Pelatihan Pemanfaatan Limbah Kulit
Nenas Sebagai Bahan Pembuatan Sabun Cuci Piring. COMSEP: Jurnal Pengabdian Kepada Masyarakat, 3(3), 332–
338. https://doi.org/10.54951/comsep.v3i3.323
Juariah, S., & Wati, D. 2021. Efektifitas Ekstrak Bonggol Nanas (Ananas comosus L. Merr) Terhadap Escherichia coli.
Meditory : The Journal of Medical Laboratory, 8(2), 95–100.
Lestari, G., & Fitri, R. D. 2019. Uji Daya Hambat Ekstrak Buah Nanas (Ananas comosus. L) Terhadap Bakteri Escherichia
coli. Jurnal Ilmiah Farmacy, 6(1), 57–65.
Lourenço, S. C., Campos, D. A., García, G. R., Pintado, M., Oliveira, M. C., Santos, D. I., Filho, C. L. C., Martins, M.
M., & Alves, V. D. Nurcholis, J., Saturu, B., Syaifuddin, & Buhaerah. 2020. Aplikasi Pupuk Organik Cair Limbah
Kulit Nenas Terhadap Pertumbuhan Tanaman Kacang Panjang. Jurnal Agrisistem, 16(2), 100–
107. http://ejournal.polbangtan-gowa.ac.id
Mosayan, G. A., Hukma S, S., & Walidah P H, H. 2022. Pinaplast: Plester Luka dari Ekstrak Bonggol Nanas (Ananas Comosus (L.) Merr.) sebagai Pengobatan Alami Luka Sayat. Jurnal Edukasi Dan Sains Biologi, 4(1), 26–33
Noviana, H. 2004. Pola kepekaan antibiotika Escherichia coli yang diisolasi dari berbagai spesimen klinis. Jurnal kedokteran
trisakti, 23(4), 122-126.
Parfati, N., & Windono, T. 2017. Sirih Merah (Piper crocatum Ruiz & Pav.) Kajian Pustaka Aspek Botani, Kandungan Kimia,
dan Aktivitas Farmakologi. MPI (Media Pharmaceutica Indonesiana), 1(2), 106–115.
Pratiwi, B. E. 2015. Isolasi dan skrining fitokimia bakteri endofit dari daun rambutan (Nephelium lappaceum L.) yang
berpotensi sebagai antibakteri (Bachelor's thesis, UIN Syarif Hidayatullah Jakarta: Fakultas Kedokteran dan Ilmu
Kesehatan, 2015).
Putri, D. A., Mawli, R. E., Safitri, A. R., Anggraini, D. A., & Kristina, M. 2024. Sosialisasi dan Aplikasi Eco-Enzyme dari
Limbah Kulit Nanas sebagai Bahan Sabun Antiseptik. Sewagati, 8(5), 2195–
2204. https://doi.org/10.12962/j26139960.v8i5.2178
Rahmawati, I., Maulida, R., & Aisyah, S. 2021. Potensi Antibakteri Sediaan Sabun Cair Ekstrak Kulit Nanas (Ananas comosus
L. Merr.) Terhadap Bakteri Staphylococcus aureus ATCC 25923. Jurnal Farmasi & Sains Indonesia, 4(2), 1–
11. https://doi.org/10.52216/jfsi.vol4no2p1-11
Rishliani, Y. R. 2022. Uji Aktivitas Antibakteri Ekstrak Etanol Daun Nanas (Ananas Comosus (L.) Merr.) Terhadap
Propionibacterium Acnes. In Jurusan Farmasi Fakultas Kedokteran Dan Ilmu Kesehatan Universitas Jambi.
Ristiani, N., Putri, Y., & Ananda, P. 2023. Optimalisasi pemanfaatan limbah kulit nanas (Ananas comosus, L) dalam
pembuatan sabun cuci piring di Desa Kubang Jaya. Jurnal Dedikasi Teknik, 3(2), 27-
31. https://doi.org/10.58794/jdt.v3i2.528.
Siddiq, N. A. A. M., Hikmawan, D. B., Hajrah, Gama, I. N., Zamruddin, M. N., Rijai, J. A., & Wijaya, V. 2023. Penyuluhan
Pemanfaatan Limbah Kulit Buah Nanas Sebagai Alternatif Pembuatan Sabun Cuci Piring Di Kelurahan Bukit Pinang,
Samarinda, Kalimantan Timur. AMMA : Jurnal Pengabdian Masyarakat, 2(9 : Oktober), 1113–
1118. https://journal.mediapublikasi.id/index.php/amma/article/view/3648
Soemarno. 2001. Isolasi dan Identifekasi Bakteri Klinik. Yogyakarta : AAK Yogyakarta. Depkesri. Hal 5-6, 22,38
Sitepu, N., Rahman, A. O., & Puspasari, A. 2022. Efektivitas antibakteri ekstrak kulit nanas (Ananas comosus) N-Heksana
terhadap bakteri Staphylococcus aureus ATCC 25923. JOMS (Jurnal of Medicine and Science), 2(1), 59-67. https://onlinejournal.unja.ac.id/joms/article/download/18093/13346.
Susanto., Sudrajat dan R. Ruga. 2012. Studi Kandungan Bahan Aktif Tumbuhan Meranti Merah (Shorea leprosula Miq)
Sebagai Sumber Senyawa Antibakteri. Jurnal Mulawarman Scientifie. Vol. 11, No. 12: 181-190
Tivani, I., & Perwitasari, M. 2021. Efektivitas Antibakteri Ekstrak Beberapa Kulit Buah Terhadap Bakteri Eschericia coli. Of
Current Pharmaceutical Sciences, 4(2), 339–346.
Authors
Copyright (c) 2026 Rica Denis, Yurike Yurike, Ari Femi Agustiyab

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
An author who publishes in Naturalis agrees to the following terms:
Author retains the copyright and grants the journal the right of first publication of the work simultaneously licensed under the Creative Commons Attribution-ShareAlike 4.0 License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal
The author is able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book) with the acknowledgement of its initial publication in this journal. The author is permitted and encouraged to post his/her work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of the published work (See The Effect of Open Access).
Submission of a manuscript implies that the submitted work has not been published before (except as part of a thesis or report, or abstract); that it is not under consideration for publication elsewhere; that its publication has been approved by all co-authors. If and when the manuscript is accepted for publication, the author(s) still hold the copyright and retain publishing rights without restrictions. Authors or others are allowed to multiply an article as long as not for commercial purposes. For the new invention, authors are suggested to manage its patent before published. The license type is CC-BY-SA 4.0.
Naturalis is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.