UJI AKTIVITAS ANTIPLASMODIUM FRAKSI N-HEKSANA DAUN Peronema canescens TERHADAP Mus musculus

Fenny Andriani, Agus Sundaryono, Nurhamidah Nurhamidah

Abstract


Peronema canescens (Sungkai) has been used in Bengkulu as raw herbal remedy to reduce the fever, some people are using as a malaria drug. P.  canescens leaves contain alkaloids, flavonoids, tannins and terpenoids – steroids .This study aims to analyze the effect of P.  canescens leaves  n-hexane fraction.  against paracetemias in Mus musculus infected with Plasmodium berghei, then to prove whether the . P.  canescens leaves n-hexane fraction  has potential as alternative medicine for malaria .   To make P. canescens extract , leaves was macerated using EtOH (96%), then filtrate was evaporated using a rotary evaporator, then fractionated with n-hexane. The 25 healthy  M.musculus  weighing 20-40g each, infected with P.berghei, grouped into 5 ie group 1 (K-) was treated orally with aquades, group 2 (K +) was treated orally with 0.42 mg of Chloroquine, Group 3 (P1) Group 4 (P2), group 5 (P3) was treated orally with a P.  canescens leaves n-hexane fraction  each at a dose of 0.028 ,  0.056 and 0.084 g / kgBW. After 3 days of digestion for each treatment, the amount of erythrocytes was calculated under a microscope. : The giving of the with dose 0,028 g / kgBW able to inhibit paracetemia 33,49%,  0,056 g / kgBW able to  57.91% and  0,084 g / kgBW able to  61.69%   The conclusion is the P. canescens leaves n-hexane fraction  at a dose of 0.028 , 0.056 , and 0.084 g / kgBW orally wil be decreased the amount of paracetemia in M.musculus, and the higher the dose given the higher percent of the inhibition.


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References


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