Main Article Content
Abstract
Xylem Limited Bacterium (XLB) disease (Ralstonia syzygii) is the most seriuos disease on clove plant. Biological control using Endophytic Bacteria (Bacillus sp. Bc25 and Fluorescentpseudomonad Pf22) is expected to control clove XLB disease.The research aim to get Bacillus sp. Bc25 and Fluorescent pseudomonad Pf22product are effective to control XLB disease on clove plant.The study of Bacillus sp. Bc25 and Fluorescent pseudomonad Pf22 was done in watering on clove plant field were infected by XLB disease in Solok West Sumatera on January to December 2017.The study used a randomized block design (RBD) with four replications. The parameters observed were the disease attack level and plant growth.The study results showed thatBacillus sp.Bc25 andFluorescentpseudomonadPf 22 can control XLB disease and increase clove growth.Bacillus sp. Bc25 and Fluorescentpseudomonad Pf22 combinationdemonstrate XLB disease control capabilities and promote the best clove plants growthby intensity suppressing of XLB disease from 86.80% to 20.59% and increasing the clove plants growth from the stem circle 20.00 cm to 46.00 cm.Combination product of biological agents Bacillus sp. Bc25 and FluorescentpseudomonadPf22 can be developed to control XLB diseases and increase clove plant growth.
Keywords : Clove, Xylem Limited Bacteria Disease (Ralstonia syzygii), Endophytic Bacteria (Bacillus sp. Bc25 and Fluorescent pseudomonad Pf22.
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Copyright (c) 2022 Chrisnawati Chrisnawati, Nasrun Nasrun, Yulfidesi Yulfidesi, Suryani Suryani, Kasmawati Kasmawati

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References
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- Guetsky, R., D., Shtienberg, Y. Elad, and A. Dinoor. 2002. Improving biological control by combining biocontrol agents each with several mechanisms of disease suppression. Phytopathology.92 : 979-985.
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- Nasrun, Nurmansyah, H.Idris and Chrisnawati. 2016. Isolation and Selection of Potential Endophytic Bacteria to Increase Clove Resistance to Xylem Limited Bacteria (XLB)Disease. Proceedings of the National Seminar on the Indonesian BIODIVERSITY Society. Volume 3 No.3 89-131.
- Premono. E. 1998. Phosphate Solubilizing Microbes to Make Phosphate Fertilizers Efficient and Their Prospects in Indonesia.Hayati.Vol 11 pp 13-23.
- Rahayu and Yuniarti. 2014. Xylem LimetedBacteria (XLB) Clove Attack in East Java Quarter 1 of 2014. Directorate General of Agriculture.
- Schippers, B., B. Lugtenberg, and P.J. Weisbeek. 1987. Plant Growth Control by Fluorescent Pseudomonass. Innovative Approaches to Plant Disease Control 30-34.
- Wahdah.S.R. 2015. Utilization of Plant Growth Promoting Rhizobacteria (PGPR) in controlling tungro disease in local rice in South Kalimantan PROSSEMNAS MASY BIODIV INDON 1 (6): 1448-1456
- Zieden.E.H.E. 2006. Manipulating Endophytic Bacteria for Biological control to soil borne disease of Peanut. Journal of Applied Sciences Research. 2(8): 497-502.
References
Akila.R; Rajendran.L; Harish.S; Saveetha.K; Raguchanderan.T; and Samiyappan.R. 2011. Combined Application of Botanical formulation and Biocontrol agents for the management of Fusarium oxysporum. f .sp. cubences (Foc) Causing Fusarium Wilt in Banana. Tamil Nadu India: Department of Plant Patholology Centre for Plant Protection Studies, Tamil Nadu Agricultural University, LawleyRoad, Colmbatore 641003.
Ardakani.S.S; A.Heydari; N.Khorasani and R.Arjmandi. 2010. Development of New Bioformulations of Pseudomonas fluorecsens and Evaluation of these Products against Damping-off of Cotton Seedlings. Journal of Plant Pathology. 91 (1): 83-88.
Boer, M.D., P., Bom, F. Kindt, J.J.B. Keurentes, L.V.D. Sluis, L.C.V, Loon, and P.A.H.M. Baker. 2003. Control of fusarium wilt of radish by combining Pseudomonas putida strains that different disease suppressive mechanisms. Phytopathology 93: 626 – 632.
Campbell., R.1989. Biological control of Microbial Plant Pathogens. Cambridge University Press. Cambridge. P.218
Guetsky, R., D., Shtienberg, Y. Elad, and A. Dinoor. 2001. Combining biocontrol agents to reduce the variability of biological control. Phytopatology.91(43): 621-627.
Guetsky, R., D., Shtienberg, Y. Elad, and A. Dinoor. 2002. Improving biological control by combining biocontrol agents each with several mechanisms of disease suppression. Phytopathology.92 : 979-985.
Khabbaz.S.E and P.A.Abbasi, 2014. Isolation, Characterization, and formulation of antagonistic bacteria for the management of seedlings dampingoff and root rot disease of cucumber. Can.J.Microbiology 60: 25-33
Maharina, K.E., Aini, L.Q., And Wardianto.T.2014. Application of biological agents and
vegetable materials as control of bacterial (Ralstonia solanaceraum) in plant cultivation
tomato. Journal of Plant Production. 1 (6); 506-513.
Muthukumar.A; R.Bhaskaran and K.Sanjeevkumar. 2010. Efficacy of Endophytic Pseudomonas fluorescens (Trevisan) migula against chilli damping-off. Journal of Biopesticides 3 (1): 105-109
Nasrun 2005.Study of Biological Control of Patchouli Bacterial Wilt (Ralstoniasolanacearum) with fluorescent Pseudomonad.Doctoral Dissertation of GadjahMada University's Postgraduate Program. 129 p
Nasrun, Christanti, T.Arwiyanto, and I.Mariska, 2007.Physiological Characteristics of Ralstoniasolanacearum Causes of Patchouli Bacterial Disease.Journal of Industrial Crops Research Journal.Center for Agriculture Research and Development, Agricultural Research and Development Agency.Bogor .. Volume 13 No.2; 43-48
Nasrun, Nurmansyah and Burhanudin, 2009. Utilization of fluorescent Pseudomonas as an Agency for Enhancing Plant Resilience in Controlling Patchouli Budog Disease Final Report of the 2009 National Education Incentive Program. Center for Plantation Research and Development. Research Institute for Medicinal and Aromatic Plants. 2008. 49pp.
Nasrun, Nurmansyah, H.Idris and Chrisnawati. 2016. Isolation and Selection of Potential Endophytic Bacteria to Increase Clove Resistance to Xylem Limited Bacteria (XLB)Disease. Proceedings of the National Seminar on the Indonesian BIODIVERSITY Society. Volume 3 No.3 89-131.
Premono. E. 1998. Phosphate Solubilizing Microbes to Make Phosphate Fertilizers Efficient and Their Prospects in Indonesia.Hayati.Vol 11 pp 13-23.
Rahayu and Yuniarti. 2014. Xylem LimetedBacteria (XLB) Clove Attack in East Java Quarter 1 of 2014. Directorate General of Agriculture.
Schippers, B., B. Lugtenberg, and P.J. Weisbeek. 1987. Plant Growth Control by Fluorescent Pseudomonass. Innovative Approaches to Plant Disease Control 30-34.
Wahdah.S.R. 2015. Utilization of Plant Growth Promoting Rhizobacteria (PGPR) in controlling tungro disease in local rice in South Kalimantan PROSSEMNAS MASY BIODIV INDON 1 (6): 1448-1456
Zieden.E.H.E. 2006. Manipulating Endophytic Bacteria for Biological control to soil borne disease of Peanut. Journal of Applied Sciences Research. 2(8): 497-502.