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Abstract
ABSTRACH
This study aims to obtain the interaction of rhizobacteria and soybean varieties, types of rizobacteria that effectively dissolve phosphate, varieties that are able to grow well on ultisol soils. The research is rizobacterial isolation and rizobacteria selection. Observations made were bacterial isolation, identification of morphological and physiological rhizobacteria (gram reactions, hypersensitive reactions, as phosphate solvents and germination test and rhizobacteria test of phosphate solvents by inplanta. The results showed that rizobacteria isolates from ultisol soil of chilli plants, eggplant plants and rice roots obtained 32 isolates. The highest number of isolates were obtained from the ultisol soil of chilli plants namely 18 isolates. The color of the rizobacteria isolate colony was obtained by 27 cream isolates and 5 yellow isolates. The surface of the colony in the chilli ultisol soil consisted of 3 groups namely arising, flat and convex, the surface of the colony arising obtained 8 isolates, the flat surface obtained 9 isolates and the convex surface obtained 1 isolate. All isolates were gram negative. From 32 rizobacteria isolates, there were 2 isolates which were able to dissolve phosphate, namely RT1 and RC3. In green bean plants with RT1 administration, the seeds weight was 2.76 grams high compared to control and RC3.
Key words: Rhizobacteria solvent phosphate RT1, RC3, ultisol, mung bean
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Copyright (c) 2020 Afri R. Diyanti, M Z.H. Utama, Milda Ernita
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References
- Faccini G, Garzon S, Martines M, & Varela A. 2004. Evaluation of the effects of a dual inoculum of phosphate-solubilizing bacteria and Azotobacter chroococcum, in creolo potato (Papa “Criolla”) (S o l a n u m p h u reya ) va r ‘Yema deHu evo’. h t t p : w w w. a g . a u b u r n . e d u / a r g e n t i n a / pdfmanuscripts/faccini.pdf . [ 17 Januari 2008].
- Hafeez FY, Yasmin S, Ariani D, Rahman M, Zafar Y, & Malik KA. 2006. Plant growth promoting bacteria as biofertilizer. J Agron. Sustain. Dev 26:143-150.
- Ilham,Ida B. G. D, Gusti M O N dan Retno K. 2014. Isolates and Identification of Potential Phosphate Solvent Bacteria in Conventional Soils and Organic Soils. Journal of Symbiosis II (1): 173-183.
- Hasnudin. 2002. Peningkatan Kesuburan Tanah dan Hasil Kedelai akibat Inokulasi Mikroba Pelarut Fosfat dan Azobakteri pada Ultisol. Jurnal Ilmu-ilmu Pertanian Indonesia. Volume4, No.2:97-103.
- Joo GJ, Kim YM, Kim JT, Rhee IK, Kim JH, & Lee IJ. 2005. Gibberellins-producing rhizoba cteria increase endogenous gibberellins content and promote growth of red peppers. J. Microbiol. 43:510-515.
- Karnwal A. 2009. Production of indole acetic acid by fluorescent Pseudomonas in the presence of L- tryptophan and rice root exudates. J. Plant Pathol. 91:61-63.
- Khamdanah, Tiara R A dan Jati P. 2014. Effectiveness of Phosphate Solubilizing Bacteria from the Lebak Ultisol Land of Banten against Soybean Growth. Proceedings of the seminar on organic agriculture journals. Bogor.
- Krisdiana, R. 2014. Distribution of Soybean Superior Varieties and Their Impact on the Rural Economy. J Food Crop Agriculture Research33:1:61-69
- Larasati .E. D, Rukmi MG. I, Kusdiyantini E, dan Ginting, R. C. B. 2018. Isolation and Identification of Phosphate Solubilizing Bacteria from Peat Soil. Biomes. 20, 1,1-8.
- Marbun , S, Sembiring M, Bintang. 2015. The Application of Microbial Phosphate Solvents and Organic Materials to Increase P Absorption and Potato Growth in Andisols Affected by Eruption of Mt. Journal of Agroecotechnology Vol.4. No.1, Desember 2015. (552) :1651 – 1658
- Maryanti, D. 2006. Isolation and test the ability of phosphate solvent bacteria from rhizosphere food plants and shrubs. [skripsi]. Padang. Andalas University Faculty of Agriculture. 84 halaman.
- Mehrvraz S & Chaichi MR. 2008. Effect of phosphate solubilizing microorganisms and phosphorus chemical fertilizer on forage and garin quality of barley. American-Eurasian J.Agric. & Environ. Sci. 3(6):855-860.
- Prasetyo, B. H dan D. A Suriadikarta. 2006. Characteristics, Potential and Technology of Ultisol Land Management for the Development of Dry Land Agriculture in Indonesia. A Agricultural Research and Development, 25(2):39-47
- Rao NSS. 2007. Microorganisms and Plant Growth. Jakarta: UI Press.
- Rohyani, Zul D, dan Fibrianti, B. L. 2014. Potential Indigenus Bacteria Isolates as Biofertilizer Agents from Peat soils in the Zambrud Region and Tesso Nilo National Park, Riau. JOM FMIPA 1(2):417-429
- Simanungkalit, R. D. M dan Suriadikarta, D. A. 2006. Organic Fertilizers and Biofertilizers. Indonesian Center for Agricultural Land Resources. Bogor.
- Sudaryanto, T dan Swastika, D. K. S. 2007. Soybean Economy in Indonesia. In: Sumarno, Suyamto, Widjono, A., Hermanto, K. H., (Eds). Soybean: Production and Development Techniques (pp. 1-27). Bogor: Agency for Research and Development of Agricultural Plants.
- Suliasih, Widawati, S dan A, Muharam. 2010. Application of Organic Fertilizers and Phosphate Solvent Bacteria to Increase Tomato Plant Growth and Soil Microbial Activity. J. Hort. 20(3):241-246
- Widiawati, S dan Suliasih. 2005. Augmentation of Phosphate Solubilizing Bacteria (BPF) as a Potential for Growth of Caysin (Brasica caventis Oed.) In Marginal Soils. J Biodiversitas 7(1):10-14.
References
Faccini G, Garzon S, Martines M, & Varela A. 2004. Evaluation of the effects of a dual inoculum of phosphate-solubilizing bacteria and Azotobacter chroococcum, in creolo potato (Papa “Criolla”) (S o l a n u m p h u reya ) va r ‘Yema deHu evo’. h t t p : w w w. a g . a u b u r n . e d u / a r g e n t i n a / pdfmanuscripts/faccini.pdf . [ 17 Januari 2008].
Hafeez FY, Yasmin S, Ariani D, Rahman M, Zafar Y, & Malik KA. 2006. Plant growth promoting bacteria as biofertilizer. J Agron. Sustain. Dev 26:143-150.
Ilham,Ida B. G. D, Gusti M O N dan Retno K. 2014. Isolates and Identification of Potential Phosphate Solvent Bacteria in Conventional Soils and Organic Soils. Journal of Symbiosis II (1): 173-183.
Hasnudin. 2002. Peningkatan Kesuburan Tanah dan Hasil Kedelai akibat Inokulasi Mikroba Pelarut Fosfat dan Azobakteri pada Ultisol. Jurnal Ilmu-ilmu Pertanian Indonesia. Volume4, No.2:97-103.
Joo GJ, Kim YM, Kim JT, Rhee IK, Kim JH, & Lee IJ. 2005. Gibberellins-producing rhizoba cteria increase endogenous gibberellins content and promote growth of red peppers. J. Microbiol. 43:510-515.
Karnwal A. 2009. Production of indole acetic acid by fluorescent Pseudomonas in the presence of L- tryptophan and rice root exudates. J. Plant Pathol. 91:61-63.
Khamdanah, Tiara R A dan Jati P. 2014. Effectiveness of Phosphate Solubilizing Bacteria from the Lebak Ultisol Land of Banten against Soybean Growth. Proceedings of the seminar on organic agriculture journals. Bogor.
Krisdiana, R. 2014. Distribution of Soybean Superior Varieties and Their Impact on the Rural Economy. J Food Crop Agriculture Research33:1:61-69
Larasati .E. D, Rukmi MG. I, Kusdiyantini E, dan Ginting, R. C. B. 2018. Isolation and Identification of Phosphate Solubilizing Bacteria from Peat Soil. Biomes. 20, 1,1-8.
Marbun , S, Sembiring M, Bintang. 2015. The Application of Microbial Phosphate Solvents and Organic Materials to Increase P Absorption and Potato Growth in Andisols Affected by Eruption of Mt. Journal of Agroecotechnology Vol.4. No.1, Desember 2015. (552) :1651 – 1658
Maryanti, D. 2006. Isolation and test the ability of phosphate solvent bacteria from rhizosphere food plants and shrubs. [skripsi]. Padang. Andalas University Faculty of Agriculture. 84 halaman.
Mehrvraz S & Chaichi MR. 2008. Effect of phosphate solubilizing microorganisms and phosphorus chemical fertilizer on forage and garin quality of barley. American-Eurasian J.Agric. & Environ. Sci. 3(6):855-860.
Prasetyo, B. H dan D. A Suriadikarta. 2006. Characteristics, Potential and Technology of Ultisol Land Management for the Development of Dry Land Agriculture in Indonesia. A Agricultural Research and Development, 25(2):39-47
Rao NSS. 2007. Microorganisms and Plant Growth. Jakarta: UI Press.
Rohyani, Zul D, dan Fibrianti, B. L. 2014. Potential Indigenus Bacteria Isolates as Biofertilizer Agents from Peat soils in the Zambrud Region and Tesso Nilo National Park, Riau. JOM FMIPA 1(2):417-429
Simanungkalit, R. D. M dan Suriadikarta, D. A. 2006. Organic Fertilizers and Biofertilizers. Indonesian Center for Agricultural Land Resources. Bogor.
Sudaryanto, T dan Swastika, D. K. S. 2007. Soybean Economy in Indonesia. In: Sumarno, Suyamto, Widjono, A., Hermanto, K. H., (Eds). Soybean: Production and Development Techniques (pp. 1-27). Bogor: Agency for Research and Development of Agricultural Plants.
Suliasih, Widawati, S dan A, Muharam. 2010. Application of Organic Fertilizers and Phosphate Solvent Bacteria to Increase Tomato Plant Growth and Soil Microbial Activity. J. Hort. 20(3):241-246
Widiawati, S dan Suliasih. 2005. Augmentation of Phosphate Solubilizing Bacteria (BPF) as a Potential for Growth of Caysin (Brasica caventis Oed.) In Marginal Soils. J Biodiversitas 7(1):10-14.