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Abstract
Oryctes rhinoceros is one of the main pests in oil palm plantations. Biological control using Metharizium anisopliae has great potential to control O. rhinoceros larvae which are usually found in organic matter. This study aims to obtain the best organic compost material for the development of M. anisopliae as biological control against O. rhinoceros larvae. This research was conducted with a completely randomized design (CRD) consisting of 6 treatments, each treatment was repeated 5 times to obtain 30 experimental units. Each unit contained 10 O. rhino larvae. The concentration of M. anisopliae was 50 g / L of distilled water. The treatments of organic media consisted of 6 setting media, as follows: Oil Palm Empty Fruit bunch (OPEFB), rice husk, sawdust, ½ part OPEFB + ½ sawdust, ½ part OPEFB + ½ part rice husk, and ½ part rice husk + ½ part sawdust. The observations included the time of initial death, time of death, daily mortality, total mortality, changes in behavior and larvae morphology. Chemical analysis of OPEFB, temperature, and humidity were recorded. The results showed that compost from sawdust media had the tendency as the best media for the development of Metarizhium anisopliae. It had ability to infect O. rhinoceros larvae which caused larvae infection 72.50%.
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Copyright (c) 2023 Hafiz Fauzana, Eka Kortima, Nelvia nelvia, Rusli Rustam, Fifi Puspita

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References
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- Saebani BA 2008. Mushroom Research Methods. Faithful Reader. Bandung
- Shegal, AK and A. Sagar, 2006. In vitro isolation and influence of nutritional conditions on the mycelial growth of the entomopathogenic and medicinal fungus cordyceps militaris. Plant Pathology Journal. Department of Bioscience. Ilimachal Pradesh University. Shimla Vol. 5 (3).
- Sinaga, S. 2010. Effect of Addition of Noni(JuiceMorinda citrifolia L) to Peroxide Numbers, Iodine Numbers for Cooking Oil. Thesis. FMIPA. USU
- Sudharto, PS, ZA Aini, CU Ginting and B. Papierok. 1998. Development of thefungus Cordyceps aff. militaris on rice bran media and its pathogenicity to the cocoon of Setothosea asigna Van Eecke. Palm oil research journal. 6 (2): 141-151.
- Suziani, W. 2011. Pathogenicity Test ofBorer Metarhizium anisopliae andFungus Cordyceps militaris on Palm-PalmLarvae.
- Winarto, L. 2005. Integrated Palm Beetle Pest Control. http://www.agroindonesia.com Accessed October 14, 2018.
- Wesi, Jasmi and Z. Lusi. 2014. Population density of horn beetles (Oryctes rhinoceros L.) In oil palm plants at PTPN VI Ophir Pasaman Barat Business Unit. Palm Oil Research Journal. 15 (2): 69-82.
- Vander, MJEC, B. Van der Veen, JCM Uitdehaag, Leemhuis , and L. Dijkhuizen, 2002. Properties and applications of starch converting enzymes of the ?amylase family) Journal of Biotechnology 94: 137–155
References
REFERENCES
Central Bureau of Statistics. 2018. Statistics of Indonesian Palm Oil. 2006 Statistics of Indonesian Palm Oil. Jakarta
Ministry of Agriculture.2012 Palm Oil Area by Province in Indonesia, 2008-2012. http://www.deptan.go.id [Downloaded July 10, 2013]
Riau Provincial Plantation Office. 2014. Palm Oil Damage Data in Riau. Oil Palm Plantation Service. Pekanbaru.
Engelkes, CA; RLNuclo & DRFravel. 1997. Effects of Carbon, Nitrogen, and C: N ratio on growth, sporulation, and biocontrol efficacy of Talaromyces flavus. Biological Control Phytopathology. Vol. 87 (5)
Freimoser, FM, S. Screen., S. Bagga, G. Hu, and RJSt. Leger. 2003. Expressed Sequence Tag (EST) analysis of two subspecies of M. anisopliae revealed a pletora secreted protein with potential activity in insects. Journal of Microbiology, 149: 239-247.
Ginting, FD 2008. Testing of Two Adsorber Cooling System Cooling Tools Using 1000ml Methanol as Refrigerant. Bachelor Thesis. Undergraduate Mechanical Engineering Study Program, University of Indonesia.
Hasyim, A. 2007. Increased infectivity of the entomopathogenic fungus Beauvoria bassina (Balsamo) Vuill. In a variety of carrier materials to control banana cobs borer Cosmopolites sordidus Germar in the field. Horti Journal. 17 (40): 335-342.
Heriyanto and Suharno. 2008. Pathogenicity Study of Metarhizium anisopliae (Meth.) Sor Results of Propagation of Natural Liquid Medium onLarvae Oryctes rhinoceros. J. Agricultural Sciences 4 (1): 47-54.
Herlinda, S., Hartono, and C. Irsan. 2008. Efficacy of Bioinsecticide Liquid Formulation withingredients Active Beauveria bassiana (Bals.) Vuill. and Metarihzium sp. On the white ridge of Sogatella furcifera Horv. PATPI National Seminar and Congress, Palembang, 14-16 Otober 2008.
Ismail, MS and AM Waliuddin. 1996. Effect of Rice Husk Ash on High Strength Concrete. Construction and Building Materials. 10 (1): 521 - 526
Kershaw, MJ, ER Moorhouse, R. Bateman, SE Reynolds, and AK Charnley. 1999. the role of destruction in the pathogenicity of Metarhizium anisopilae for three species of insect. Journal of Invertebrate Pathology 74; 213-223
Prayogo Y. 2004. Utilization of entomopathogenic fungus Metarhizium anisopliae (Metsch.) Sorokin to control pest Spodoptera litura armyworm in soybean. [Integrated Pest Control Colloquium]. Department of Pests and Plant Diseases. Bogor Agricultural University Postgraduate School. 23 p.
Prayogo Y. 2006. Efforts to maintain the effectiveness of entomopathogenic fungi to control food crop pests. Agricultural Research and Development Journal 22 (2).
Saebani BA 2008. Mushroom Research Methods. Faithful Reader. Bandung
Shegal, AK and A. Sagar, 2006. In vitro isolation and influence of nutritional conditions on the mycelial growth of the entomopathogenic and medicinal fungus cordyceps militaris. Plant Pathology Journal. Department of Bioscience. Ilimachal Pradesh University. Shimla Vol. 5 (3).
Sinaga, S. 2010. Effect of Addition of Noni(JuiceMorinda citrifolia L) to Peroxide Numbers, Iodine Numbers for Cooking Oil. Thesis. FMIPA. USU
Sudharto, PS, ZA Aini, CU Ginting and B. Papierok. 1998. Development of thefungus Cordyceps aff. militaris on rice bran media and its pathogenicity to the cocoon of Setothosea asigna Van Eecke. Palm oil research journal. 6 (2): 141-151.
Suziani, W. 2011. Pathogenicity Test ofBorer Metarhizium anisopliae andFungus Cordyceps militaris on Palm-PalmLarvae.
Winarto, L. 2005. Integrated Palm Beetle Pest Control. http://www.agroindonesia.com Accessed October 14, 2018.
Wesi, Jasmi and Z. Lusi. 2014. Population density of horn beetles (Oryctes rhinoceros L.) In oil palm plants at PTPN VI Ophir Pasaman Barat Business Unit. Palm Oil Research Journal. 15 (2): 69-82.
Vander, MJEC, B. Van der Veen, JCM Uitdehaag, Leemhuis , and L. Dijkhuizen, 2002. Properties and applications of starch converting enzymes of the ?amylase family) Journal of Biotechnology 94: 137–155