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Abstract
Long-term use of chemical pesticides in high doses might result in chemical residues. Therefore, eco-friendly plant pest control should be getting more attention. To do so, one might consider the utilization of microorganisms that have the enzymatic ability to destroy the pest's body structure. This study aimed to explore the isolates of Bacillus sp. from coffee beans as a plant pest control candidate. Bacillus could be considered due to its ability to produce protein crystals and extracellular enzymes. Three isolates are obtained from isolation from coffee beans: Bacillus sp. 1; Bacillus sp. 2; and Bacillus sp. 3. These isolates have the enzymatic character of protease, chitinase, and lipase. The results of the bioassay test on larvae of Plutella xylostella (Lepidoptera order) showed that these isolates were able to cause larvae death within 48 hours.
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Copyright (c) 2023 Christina Nugroho Ekowati, Maria Denada Siallagan, Sumardi Sumardi, Emantis Rosa
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References
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- Agustien, A. 2010. Protease Bakteri Termofilik. UNPAD PRESS. .
- Bestari, N.C., dan Suharjono. 2015. Uji Kualitatif dan Kuantitatif Isolat Bakteri Lipolitik dari Limbah Cair Pabrik Pengolahan Ikan Kecamatan Muncar Banyuwangi. Jurnal Biotropika, 3(3):151 -155
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- Federici, Brian A., Hyun-Woo Park, dan Yuko Sakano. 2006. Insecticidal Protein Crystals of Bacillus thuringiensis. Microbial Monogr. doi: 10.1007/7171.
- Firliani, W., A. Agustien, and F.A Febria. 2015. Karakterisasi Bakteri Termofilik Penghasil Enzim Protease Netral. Journal Biologi Universitas Andalas 4(1):9-14
- Gupta, R., Q. K. Beg and P. Lorenz. 2002. Bacterial alkaline proteases: Molekuler approaches and industrial applications. Applied Microbial Biotechnology, 59: 15 - 32
- Nurdin, G. M, Mubarik, N. R, Sudirman Li. 2016. Selection of chitinolytic bacteria as biological control of Colletotrichum capsici. Malaysian Journal of Microbiology.12(1): 35-42
- Siallagan, M. D., Ch. N. Ekowati, Sumardi, dan E. Rosa. 2020. Deteksi Kristal Protein Pada Isolat Bacillus sp. dengan Pewarnaan Coomassie Brilliant Blue. Biospecies, 13(2).
References
REFERENCES
Agustien, A. 2010. Protease Bakteri Termofilik. UNPAD PRESS. .
Bestari, N.C., dan Suharjono. 2015. Uji Kualitatif dan Kuantitatif Isolat Bakteri Lipolitik dari Limbah Cair Pabrik Pengolahan Ikan Kecamatan Muncar Banyuwangi. Jurnal Biotropika, 3(3):151 -155
Cavaglieri L., Andres L., Ibanez M., Etcheverry M.. 2005. Rhizobacteria and their potential
tocontrol Fusarium verticillioides:effect of maize bacterisationand inoculum density.
Antonie Van Leeuwenhoek.
Federici, Brian A., Hyun-Woo Park, dan Yuko Sakano. 2006. Insecticidal Protein Crystals of Bacillus thuringiensis. Microbial Monogr. doi: 10.1007/7171.
Firliani, W., A. Agustien, and F.A Febria. 2015. Karakterisasi Bakteri Termofilik Penghasil Enzim Protease Netral. Journal Biologi Universitas Andalas 4(1):9-14
Gupta, R., Q. K. Beg and P. Lorenz. 2002. Bacterial alkaline proteases: Molekuler approaches and industrial applications. Applied Microbial Biotechnology, 59: 15 - 32
Nurdin, G. M, Mubarik, N. R, Sudirman Li. 2016. Selection of chitinolytic bacteria as biological control of Colletotrichum capsici. Malaysian Journal of Microbiology.12(1): 35-42
Siallagan, M. D., Ch. N. Ekowati, Sumardi, dan E. Rosa. 2020. Deteksi Kristal Protein Pada Isolat Bacillus sp. dengan Pewarnaan Coomassie Brilliant Blue. Biospecies, 13(2).