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Abstract
Generative propagation of orchid plants has problems because orchid seeds do not have endosperm that need to be multiplied using tissue culture. Contamination is a limiting factor in the multiplication of plants in tissue culture. This study describes the types of contaminants found in Bromheadia finlaysoniana callus. This research was carried out in two stages. The first stage is carried out culture of various explants of Bromheadia finlaysoniana using murashige and Skoog media with enrichment of hormone Benzylaminopurine 1 mg/l; (Naphthalene acetic acid) 0.5 mg/l; 100 mg mynositol; pyridoxine-HCl 0.5 mg/l; thiamine-HCl 0.1 mg/l; nicotinic-acid 0.5 mg/l; glycine 2 mg/l. The second stage is observed and the percentage of contaminant fungi. The observed contaminants character includes the colour, the direction of growth, and the hyphae colony's shape. Determination of contaminants type compared to the morphology of references. The result is the highest contamination in flower stalk explants, 81%; most colonies on leaf explants are 28. Contaminant fungi grow predominantly of fungi with white and grey colour and a rough surface shape. The macroscopic character of contaminant fungi in tissue cultures mostly comes from the class of Deuteromycetes and Zygomycetes.
Keywords: Bromheadia finlaysoniana , contaminant, fungi, tissue culture
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Copyright (c) 2021 Desta Desta Andriani, Pebra Heriansyah
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References
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References
Andersen, T. F. & H. N. Rasmussen. (1996). The Mycorrhizal Species of Rhizctonia. In Sneh, B., S. Jabaji Hare, S. Neate, & G. Dijst (Eds). Rhizoctonia Species: Taxonomy, Molecular Biology, Ecology, Pathology and Disease Control. (pp. 379-390). London: KAP.
Basri, Z. 2004. Kultur Jaringan Tanaman Universitas Tadulako Press. Palu.
Bey, Y., W. Syafii, dan N. Ngatifah. 2006. Effect of Giberelin Giving on Vacint and Went media on the germination of Moon Orchid Seeds (Phalaenopsis amabilis BL) in In Vitro Jurnal Biogenesis.vol 14,no. 1,:15-21
Gunawan, L.W. 2004. Budidaya Anggrek. Penebar Swadaya. Jakarta.
Juarna K.S 2016. Contamination explan Centella asiatica (L.) Urban (Pegagan) In Vitro culture through comparison of two sterilization methods. Jurnal Pro-Life. 3: 2.119-128
Murashige, T. & Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15, 472-497.
Oratmangun K.M, Pandiangana D * , Febby E. Kandou F.E. 2017. Description Types of Contaminants From Culture Callus Catharanthus roseus (L.) G. Don. Jurnal MIPA Unsrat Online.6 : (1). 47--52
Puspitaningtyas D. M. 2009. exploration of plants in the Suaka Margasatwa Bukit Rimbang Baling. Paper presented at Proceedings of Seminar keanekaragaman hayati. Maliki, Malang. Malang, Indonesian
Waluyo, L., 2004, Mikrobiologi Umum, Malang, UMM press
Wati, T , Astarini I.A, Pharmawati M, Hendriyani E. 2020. Propagation Of Begonia Bimaensis Undaharta & Ardaka Using Tissue Cultura Technique. Journal of Biological Sciences 7(1): 112-122. DOI: 10.24843/metamorfosa.2020.v07.i01.p15