Article Sidebar

Download
PDF
Statistic
Read Counter : 132 Download : 94

Main Article Content

Abstract

Cryopreservation is an important ex-situ conservation measure practiced successfully in fishes over the years. The effects of different extenders and cryoprotectants on the viability and fertilizing ability of cryopreserved sperm of Mystus vittatus were investigated in this study in order to develop a cryopreservation protocol. Milt was collected through sacrifice of males and was suspended in extender solutions. The concentration and pH of milt were found to be 7.9×109 - 8.1×109cells/ml and 8±0, respectively. Different concentrations of NaCl solutions (0.1% to 1.1%) were used to evaluate activation of sperm motility and it was decreased as the concentration of the NaCl solution increased. Sperm motility was completely inhibited at 1.1% and 0.8% NaCl solution with Alsever’s solution and Kurokura-2 solution respectively. Two extenders, Alsever’s and Kurokura-2 solutions and two cryoprotectants, Dimethyl sulfoxide (Me2SO) and methanol were employed to preserve the sperm. Ten percent cryoprotectants with both extenders, Alsever’s and Kurokura-2 solutions produced better motility after 5 and 10 min incubation. 15% cryoprotectant was found to be toxic to sperm. Alsever’s solution with 10% Me2SO showed better performance producing 77.5±1.4% and 58.8±1.25% equilibration and post-thaw motility than that of 73.8±3.15% and 52.5±2.5% with Kurokura-2 solution plus Me2SO respectively. Between two diluents, sperm preserved with Alsever’s solution plus Me2SO produced highest fertilization (70.0±7.07%) and hatching (37.5±13.7%), while those preserved with Kurokura-2 plus Me2SO produced 72.5±2.5% and 29.9±12.5% fertilization and hatching respectively. Fresh sperm yielded 85.0±0% fertilization and 48.0±15.5% hatching.  The protocol developed through this study can be applied for long-term preservation of genetic materials of the threatened catfish M. vittatus and the cryopreserved sperm can be used in artificial breeding for broodstock development. Thus the protocol will help to propagate the new generation in hatcheries using cryopreserved sperm and make the fish available in captive culture systems as well as in wild which eventually helps to eradicate poverty.

Article Details

License

Authors who publish with this journal agree with the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
  4. This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

References

  1. Ashwood-Smith, M.J. 1980. Low temperature preservation of cells, tissues, and organs. In: M.J. Ashwood -Smith and J. Farrant, (editors). Low temperature preservation in medicine and biology (pp.19-44). Pittman Medical, Tunbridge Wells, Kent, UK.
  2. Armitage, W. J. 1987. Cryopreservation of animal cells. Symp. Soc. Exp. Biol. 41: 379 –393. Bart, A. 2002. Conservation of ?sh genetic diversity: need for development of a cryogenic gene bank in Bangladesh. In: D. J. Penman, M. G. Hussain, B. J. McAndrew, M. A. Mazid (Eds). Proceedings of a workshop on genetic management and improvement strategies for exotic carps in Asia, 12-14 February 2002 (pp. 107–110). Bangladesh Fisheries Research Institute, Mymensingh, Dhaka, Bangladesh.
  3. Baynes, S.M., A.P. Scott, and A.P. Dawson. 1981. Rainbow trout, Salmo gairdnerii Richardson, spermatozoa: Effect of cations and pH on motility. Journal of Fish Biology 19(3): 259–67.
  4. Billard, R., Cosson, J., Crim, L.W., Suquet, M. 1995. Sperm Physiology and Quality. In: Broodstock Management and Egg and Larval Quality (pp. 25-52). Blackwell Science, Oxford.
  5. Billard, R., J. Cosson, S.B. Noveiri, and M. Pourkazemi. 2004. Cryopreservation and short-term storage of sturgeon sperm. Aquaculture 236 (1/4): 1-9.
  6. Buda, C.S., I. Dey, and T. Farkas. 1996. Role of membrane lipids in temperature acclimatization of carp. Archive of Animal Nutrition 49(1): 61–62.
  7. Cabrita, E., S. Sarasquete, S. Martinez-Paramo, V. Robles, J. Beirao, S. Perez-Cerezales, and M.P. Harraez. 2010. Cryopreservation of fish sperm: Applications and prospective. Journal of Applied Ichthyology 26(5): 623-635.
  8. Chao, N.H., W.C. Chao, K.C. Lui, and I.C. Liao. 1987. The properties of tilapia sperm and its cryopreservation. Journal of Fish Biology 30(2): 107-118.
  9. Chao, N., H.P. Chen and I. Chiuliao. 1975. Study on cryogenic preservation of grey mullet sperm. Aquaculture 5: 389-406.
  10. Ciereszko, A., L. Ramseyer, and K. Dabrowski. 1993. Cryopreservation of yellow perch semen. The Progressive Fish Culturist 55(4): 261–264.
  11. Craig, J. F., A.S. Halls, J.J.F. Barr, and C.W. Bean. 2004. The Bangladesh floodplain fisheries. Fisheries Research 66(2-3): 271-286.
  12. Daly, J., D. Galloway, W. Bravington, M. Holland, and B. Ingram. 2008. Cryopreservation of sperm from Murray cod, Maccullochella peelii. Aquaculture 285(1): 117-122.
  13. Daniels, R.J.R. 2002. Freshwater fishes of Peninsular India. Universities Press, Hyderabad, India, 288.
  14. DoF. 2016. Fish Week Compendium (In Bengali). Department of Fisheries, Ministry of Fisheries and Livestock, Matshya Bhaban, Dhaka.
  15. Dong, Q., C. Huang, and T.R. Tiersch. 2007. Control of sperm solution is necessary for standardization of sperm cryopreservation in aquatic species: evidence from sperm agglutination in oysters. Cryobiology 54(1): 87- 98.
  16. FAO. 2014. The State of World Fisheries and Aquaculture. Food and Agriculture Organization of the United Nations, Rome.
  17. Farkas, T., E. Fodor, K. Kitajka, and J.E. Halver. 2001. Response of fish membranes to environmental temperature. Aquaculture 32(8): 645–55.
  18. Figueroa, E., I. Valdebenito, A.B. Zepeda, , C.A. Figueroa, K. Dumorne, R.L. Castillo, and J.G. Farias. 2015. Effects of cryopreservation on mitochondria of fish spermatozoa. Reviews in Aquaculture 9 (1): 76-87.
  19. Gupta, S. and S. Banerjee. 2012. Indigenous ornamental fish: a new boon in ornamental fish trade of West Bengal. Fishing Chimes 32(1): 130-134.
  20. Gupta, S. and S. Banerjee. 2014. Indigenous ornamental fish trade of West Bengal. Narendra Publishing House, New Delhi, 63.
  21. Horvath, A. and B. Urbanyi. 2000. The effect of cryoprotectants on the motility and fertilizing capacity of cryopreserved African catfish Clarias gariepinus (Burchell 1822) sperm. Aquaculture Research 31 (3): 317-324.
  22. Hossain, M.Y., Z. F. Ahmed, P.M. Leunda, S. Jasmine, J. Oscoz, and R. Miranda. 2006. Length-weight and length-length relationships of the Asian striped catfish Mystus vittatus (Bloch, 1794) (Siluriformes: Bagridae) in the Mathabhanga River, Southwestern Bangladesh. Journal of Applied Ichthyology 22 (4): 304-307.
  23. Hossain, M.Y. 2014. Threatened fishes of the world: Mystus vittatus. Croatian Journal of Fisheries 72: 183-185
  24. IUCN. 2003. Book on Threatened Wild Animals of Bangladesh. Eds. M.A. Islam, M. Ameen, and A. Nishat. The World Conservation Union. Dhaka, Bangladesh.
  25. Kibria, G. and K.K.U. Ahmed. 2005. Diversity of selective and non-selective fishing gear and their impact on inland fisheries in Bangladesh. NAGA, ICLARM Quarterly 28(1-2): 43-48.
  26. Kumar, K. 1988. A comparative study of various extenders for cryopreservation of carps spermatozoa. Indian Journal of Animal Sciences 58(11): 1355-1360.
  27. Kumar, K. 1989. Studies on cryogenic preservation of carp spermatozoa In: Das and Jhingran (eds.) Fish Genetics in India (pp.199-206).Today and Tomorrows Printers and Publishers, New Delhi.
  28. Lahnsteiner, F., B. Berger, A. Horvath, B. Urbanyi, and T. Weismann. 2000. Cryopreservation of spermatozoa in cyprinid fishes. Theriogenology 54(9):1477–1498.
  29. Leung, L.K.P. 1987. Cryopreservation of spermatozoa of the barramundi, Lates calcarifer (Teleostei: Centropomidae). Aquaculture 64(3): 243-247.
  30. Linhart, O. and M. Rodina. 2000. Cryopreservation of common carp (Cyprinus carpio) and Tench (Tinca tinca) sperm for gene resources conservation. International Symposium on the Reproductive Physiology of Fish 23: 402-404.
  31. Mazur, P. 1984. Freezing of living cells: Mechanisms and implications. American Journal of Physiology 247(3): C125-42.
  32. Mongkonpunya, K., T. Pupipat, and T.R. Tiersch. 2000. Cryopreservation of sperm of Asian catfishes, including the endangered Mekong giant catfish. In: T.R. Tiersch and P.M. Mazik (eds). Cryopreservation in Aquatic Species (pp.108-116). The World Aquaculture Society, Baton Rouge, Louisiana, USA.
  33. Morisawa, M., K. Suzuki, and S. Morisa. 1983. Effects of potassium and osmolality on spermatozoa motility of salmonid fishes. The Journal of Experimental Biology 107(1):105-13.
  34. Muchlisin, Z.A., R. Hashim, and A.S.C. Chong. 2004. Preliminary study on the cryopreservation of tropical bagrid catfish (Mystus nemurus) spermatozoa; the effect of extender and cryoprotectant on the motility after short-term storage. Theriogenology 62(1): 25–34.
  35. Nahiduzzaman, M., M.M. Hassan, U.H. Khanam, S.N.A. Mamun, M.A.R. Hossain, and T.R. Tiersch. 2011. Sperm cryopreservation of the critically endangered olive barb (Sarputi) Puntius sarana (Hamilton,1822). Cryobiology 62(1): 62-67.
  36. Ross, N., M. Islam, and S.H. Thilsted. 2003. Small indigenous fish species in Bangladesh: Contribution to vitamin A, calcium and iron intakes. Journal of Nutrition 133(11):4021-4026.
  37. Sarder, M.R.I., M.F.M. Sarker, and S.K. Saha. 2012. Cryopresevation of sperm of an indigenous endangered fish species Nandus nandus (Hamilton 1822) for ex-situ conservation. Cryobiology 65(3): 202-209.
  38. Sarder, M.R.I., S.K. Saha, and M.F.M. Sarker. 2013. Cryopresevation of sperm of an indigenous endangered fish, Pabda Catfish Ompok pabda. North American Journal of Aquaculture 75(1): 114-123.
  39. Shirohara, J., J.T. Canto, and J.M.F. Almendras. 1982. A comparative study of various extenders for milk fish, Chanos chanos (Forssakal) sperm preservation. Aquaculture 28(3-4): 339-346.
  40. Talwar, P. K. and A.G. Jhingran. 1991. Inland fishes of India and adjacent countries.Vol-1 and Vol-2. Oxford and IBH Publishing Co. Pvt. Ltd. New Delhi, Bombay, and Calcutta.
  41. Tiersch, T.R. 2000. Introduction. In: T.R. Tiersch and P.M. Mazik (eds). Cryopreservation in Aquatic Species (pp.19-26). World Aquaculture Society, Baton Rouge, LA.
  42. Tsuji, M., K. Ikeda, and H. Ohta. 2000. Changes in ionic environmental inducing ayu spermatozoa motility. Nippon Suisan Gakkaishi 66(1): 55–61.
  43. Urbanyi, B., A. Horvath, Z. Varga, L. Horvath, I. Magyary, and F. Radics. 1999. Effect of extenders on sperm cryopreservation of African catfish, Clarias gariepinus (Burchell). Aquaculture Research 30 (2): 145-151.
  44. Yang, H., C. Carmichael, Z.M. Varga, and T.R. Tiersch. 2007. Development of a simplified and standardized protocol with potential for high-throughput for sperm cryopreservation in Zebrafish Danio rerio. Theriogenology 68(2): 128-136.
  45. Yang, H., M. Norris, R. Winn, and T.R. Tiersch. 2010. Evaluation of cryoprotectant and cooling rate for sperm cryopreservation in the euryhaline fish medaka Oryzias latipes. Cryobiology 61(2): 211-219.
  46. Zhang, T. and D.M. Rawson. 2002. Studies of on cryopreservation of lucgene transfected blue gill sunfish fibroblast cell line. Cryoletters 23(3): 191-196.