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Abstract

This research aims to identify secondary metabolite compounds in Talas kemumu leaf stalk (Colocasia gigantea Hook.f) and to determine the effect of extract on the number of male Mus musculus leukocytes that have been induced aspirin. Aspirin is used to increase the number of M.musculus leucocytes.The method used in this research is the method of extraction by maceration to attract secondary metabolites of active compounds  C.gigantea leaf stalk and the measurement of blood leukocyte counts are calculated using a hemocytometer (improved neubauer). Test of the effect of the leaf stalk of C.giganteaextract on the number leukocytes of M.musculus  was performed by dividing into 5 groups with 5 replications according to the Federer formula, namely K (-) were given distilled water, K (+) were given aspirin, P1, P2 and P3 are each given a dose of extract of  C.gigantea leaf stalk of 0,028 g/KgBB, 0,056 g/KgBB and 0,084 g/KgBB. The results showed that the identification of phytochemical profile in C.gigantea leaf stalk contains secondary metabolite compounds such as alkaloids, flavonoids, terpenoids, tannins and saponins. The results shows that giving of C.gigantea leaf stalk extract can reduce the number of leukocytes M.musculus which have induced aspirin. The giving of C.gigantea leaf stalk extract can decrease the number of aspirin-induced M.musculus leukocytes. This is evident from the results of One Way Anova analysis and the Smallest Real Difference Test (BNT) because in each treatment group has a significant influence. The effective dose that has the greatest activity in decreasing the number of M.musculus leukocytes found in the P1 treatment of 0,028 g /KgBB can decrease the blood leukocyte count by 6,830 / mm3.

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How to Cite
Asri, R., Handayani, D., & Sundaryono, A. (2019). PROFIL FITOKIMIA DAN PENGARUH EKSTRAK TANGKAI DAUN TALAS KEMUMU (Colocasia gigantea Hook.f ) TERHADAP JUMLAH LEUKOSIT Mus musculus. ALOTROP, 3(1). https://doi.org/10.33369/atp.v3i1.9038
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References

  1. Amir, H , Bambang Gonggo Murcitro, AS Ahmad, Murni Nur Islamiah Kassim ,The Potential Use Of Phaleria macrocarpa Leaves Extract As An Alternative Drug For Breast Cancer Among Women Living In Poverty, Asian Journal For Poverty Studies (AJPS), 2017, 3(2): 138 – 145.
  2. Salim, M., Elida Mardiah, Febby Febrizal, Pengaruh Hidrolisis Enzimatis Terhadap Produksi Bioetanol Dari Umbi Talas (Colocasia gigantea Hook F), J. Ris. Kim, 2012: 5(2): 137-142.
  3. Ansori, M.R., Talas (Colocasia esculenta [L.] Schott) sebagai Obat Herbal untuk Mempercepat Penyembuhan Luka, J Agromed Unila, 2015: 2(2): 108-112.
  4. Sari, I.P., Endang Lukitaningsih, ,Rumiyati, Irfan Muris Setiawan., Indek Glikemik Uwi, Gadung Dan Talas Yang Diberikan Pada Tikus , Trad. Med. J., 2013: 18(3) : 127-131.
  5. Devi, Bonika N. and Ganesh C. Jagetia. Anticancer Activity of Colocasia gigantea (Blume) Hook. f. In Cultured Cell Lines. International Journal of Current Engineering and Scientific Research. 2017: 4 (9): 1-11
  6. Wijaya, B, A., Gayatri Citraningtyas, Frenly Wehantouw., Potensi Ekstrak Etanol Tangkai Daun Talas (Colocasia esculenta [L]) Sebagai Alternatif Obat Luka Pada Kulit Kelinci (Oryctolagus cuniculus), PHARMACON Jurnal Ilmiah Farmasi–UNSRAT,2014: 3(3): 211-219.
  7. Mohamad.H,Andriani.Y, Kamariah. B., Siang. C.C., Syamsumir, D.F., Alias, A., Radzi, S.A.M., Effect of drying method on anti-microbial , anti-oxidant activities and isolation of bioactive coumpounds from Peperomia pellucida (L) Hbk, Journal of Chemical and Pharmaceutical Research, 2015 : 7(9): 578-584.
  8. Fitryadi, K., Sutikno , Pengenalan Jenis Golongan Darah Menggunakan Jaringan Syaraf Tiruan Perceptron, Jurnal Masyarakat Informatika, 2017: 7 (1): 1-10.
  9. Kurniasih, Y., Reskiani Mulyani., Gambaran Eritrosit Pada Sdiaan Darah Tepi Pasien Malaria Di Puskesmas Sungai Pencur, Jurnal Endurance , 2018: 3(2): 226-231.
  10. Rafdinal, I., Amiruddin, , Nuzul Asmilia, Zuraidawati, , Arman Sayuti, Zuhrawati, Razali Daud, Perbedaan Jumlah Leukosit Setelah Transplantasi Kulit Secara Autograft Dan Isograft Pada Anjing Lokal (Canis lupus familiaris), Jurnal Medika Veterinaria , 2016: 10 (2): 144-146.
  11. Andriyanto, E., Pengenalan Penyakit Darah Pada Citra Darah Menggunakan Logika Fuzzy, Jurnal JITIKA, 2011: 5(2) : 1-7
  12. Rendra, M., Rismawati Yaswir. Akmal M. Hanif. Gambaran Laboratorium Leukimia Kronik di Bagian Penyakit dalam RSUP Dr. Djamil Padang. Jurnal Kesehatan Andalas, 2013: 2 (3): 141-145.
  13. Amir, H, Bambang Gonggo Murrcitro, , Uji Microtetrazolium (MTT) Ekstrak Metanol Daun Phaleriamacrocarpa (Scheff.) Boerl Terhadap Sel Kanker Payudara MCF7 , Alotrop, 2017: 1 (1) : 27-32.
  14. Shinta R.,N., Bakti Surarso, Terapi Mual Muntah Pasca Kemoterapi, Jurnal THT – KL, 2016: 9(2): 74 – 83.
  15. Hasanah, S.N., Lucie Widowati., Jamu Pada Pasien Tumor/ Kanker sebagai Terapi Komplementer, Jurnal Kefarmasian Indonesia, 2016: 6 (1): 49-59
  16. Agustina, W., Nurhamidah, Dewi Handayani , Skrining Fitokimia Dan Aktivitas Antioksidan Beberapa Fraksi Kulit Batang Jarak (Ricinus communis L.), Alotrop , 2017: 1(2) :117-122 .
  17. Rahayu ,S., Nunung Kurniasih, Vina Amalia., Ekstraksi Dan Indentifikasi Senyawa Flavonoid Dari Limbah Kulit Bawang Merah Sebagai Anti Oksidan Alami, al Kimiya, 2015: 2 (1): 1-8.
  18. Dwisari, F., Harlia, Andi Hairil Alimuddin, Isolasi Dan Karakterisasi Senyawa Terpenoid Ekstrak Metanol Akar Pohon Kayu Buta-buta (Excoecaria agallocha L.), JKK, 2016 : 5(3): 25-30.
  19. Ergina, Siti Nuryanti dan Indarini Dwi Pursitasari, Uji Kualitatif Senyawa Metabolit Sekunder Pada Daun Palado (Agave angustifolia) Yang Diekstraksi Dengan Pelarut Air Dan Etanol, J. Akad. Kim, 2014: 3(3): 165-172.
  20. Liem, A.F., Elizabeth Holle. Ivone Y Gemnafle , Sarah Wakum , Isolasi Senyawa Saponin dari Mangrove Tanjang (Bruguiera gymnorrhiza) dan Pemanfaatannya sebagai Pestisida Nabati pada Larva Nyamuk, Jurnal Biologi Papua, 2013: 5(1): 27–34.
  21. Saridewi, M.N., Meiskha Bahar, Anisah., Uji Efektivitas Antibakteri Perasan Jus Buah Nanas (Ananas comosus) Terhadap Pertumbuhan Isolat Bakteri Plak Gigi di Puskesmas Kecamatan Tanah Abang Periode April 2017, Biogenesis, 2017: 5 (2): 104-110.
  22. Raupong, M. Saleh AF, Hasruni Satya Taruma, Penaksiran Rataan Dan Varians Populasi Pada Sampel Acak Terstratifika Dengan Auxiliary Variable , JMSK, 2015: 12 (1): 9-18.
  23. April. H. Wardhana, E. Kencanawati, Nurmawati , Rahmaweni, C.B. Jatmiko , Pengaruh Pemberian Sediaan Patikan Kebo (Euphorbia Hirta L) Terhadap Jumlah Eritrosit, Kadar Hemoglobin , Dan Nilai Hematokrit Pada Ayam Yang Diinfeksi Dengan Eimeria Tenella, Jurnal Ilmu Ternak dan Veteriner, 2001: 6 (2): 126-133.
  24. Hervidea, R., Endang Linirin Widiastuti, Endang Nurcahyani, Sutyarso , G.Nurgoho Susanto, Efek Ekstrak Metanol Makroalga Cokelat (Sargassum sp.), Merah (Gracillaria sp.) dan Taurin Terhadap Gambaran Histopatologi Hepar Mencit Jantan (Mus musculus) yang Diinduksi Benzo(?)Piren, Jurnal Biologi Indonesia , 2018: 14(1): 123-131.
  25. Risky, T.A., Suyatno, Aktivitas Antioksidan Dan Anti Kanker Ekstrak Metanol Tumbuhan Paku, Adiantum philippensis L, UNESA Journal of Chemistry , 2014: 3 (1): 89-95.
  26. Rumagit, H.M., Max R.J. Runtuwene, Sri Sudewi, Uji Fitokimia Dan Uji Aktivitas Anti Oksidan Dari Ekstrak Etanol Spons Lamellodysidea herbacea, PHARMACON Jurnal Ilmiah Farmasi – UNSRAT, 2015: 4 (3): 183-192.
  27. Romadanu, Siti Hanggita Rachmawati, Shanti Dwita Lestari, Pengujian Aktivitas Antioksidan Ekstrak Bunga Lotus (Nelumbo nucifera) , FishtecH, 2014:3(1): 1-7.
  28. Agustina, S., Ruslan, Agrippina Wiraningtyas., Skrining Fitokimia Tanaman Obat Di Kabupaten Bima, Cakra Kimia (Indonesian E-Journal of Applied Chemistry) , 2016: 4 (1): 71-76.
  29. Andriani Y. Toksisitas Fraksi Aktif Steroid Ekstrak Daun Jati Belanda (Guazuma Ulmifolia Lamk.) Terhadap Aktivitas Serum Glutamat Oksalat Transaminase (SGOT) Dan Serum Glutamat Piruvat Transaminase (SGPT) Pada Tikus Putih, Jurnal Gradien, 2008 : 4 (2): 365-371.
  30. Ningsih,D.R., Zusfahair, Dwi Kartika., Identifikasi Senyawa Metabolit Sekunder Serta Uji Aktivitas Ekstrak Daun Sirsak Sebagai Anti Bakteri, Molekul, 2016: 11(1): 101 - 111
  31. Andriani, Y., Pengaruh ekstrak daun jati belanda (Guazuma ulmifolia Lamk.) terhadap bobot badan kelinci yang diberi pakan berlemak, Jurnal Gradien, 2005: 1(2): 74-76.
  32. Andriani, Y, Habsah Mohamad, M.N.I, Kassim., N.D. Rosnan., D.F. Syamsumir., J.Saidin., et al , Evaluation on Hydnophytum formicarum Tuber from Setiu Wetland (Malaysia) and Muara Rupit (Indonesia) for Anti bacterial and Antioxidant activities and anti-cancer Potency against MCF-7 and HeLa Cell. Journal of Applied Pharmaceutical Science, 2017: 7 (9) :30-37.
  33. Yosie Andriani, NHM Lazim, A Asari, F Mohamad, TST Muhammad, N Ismail, M Taib, H Amir, A Ahmad, Habsah Mohamad., Evaluation of selected echinoderms from peninsular Malaysia for cytotoxicity against HepG2 cells, antioxidant and antibacterial activities, and their metabolites profiling, Journal of Applied Pharmaceutical Science,2018: 8 (10): 32-38
  34. A.Mohamad,H., Rosmiati., Muhammad, T.S.T., Andriani,Y., Bakar, K., Ismail,N., Saidin,J., Latip, J., Musa, N., Perenrengi., Potential Secondary Metabolites from Marine Sponge Aaptos aaptos for Artherosclerosis and Vibriosis Treatments, NPC( Natural Product Communications), 2017 : 12 (8): 1227-1230.
  35. Andriani , Y, Leni Marlina , Habsah Mohamad , Hermansyah Amir , Siti Aisha M Radzi , Jasnizat Saidin , Anti –Inflammantory Activity Of Bacteria Associated With Marine Sponge (Haliclona Amboinensis) Via Reducting No Production And Inhibiting Cyclooxygenase1-Cyclooxygenase-2, And Secretory Phospholipase A2 Activities , Asian J Pharm Clin Res, 2017: 10 (11): 95-100.
  36. Musa ,N. S, Nadia Madiha Ramli, Jaznizat Saidin,Yosie Andriani, Antioxidant And Cytotoxicity Propertise Of Ethyl Acetate Fractions Of Pandanus tectorius Fruit Against HELA Cell Line, Alotrop , 2017: 1(2): 106-112.
  37. Tonahi, J.M.M., Siti Nuryanti, Suherman, Antioksidan Dari Daun Sirih Merah (Piper crocatum), J. Akademika Kim, 2014: 3(3): 383-389.