PROFIL FITOKIMIA DAN UJI SITOTOKSIK EKSTRAK ETANOL TUMBUHAN Zanthoxylum acanthopodium DC (ANDALIMAN) MENGGUNAKAN METODE BSLT

Liis Panggabean(1), Nurhamidah Nurhamidah(2), Dewi Handayani(3),
(1) FKIP Universitas Bengkulu, Indonesia
(2) FKIP Universitas Bengkulu, Indonesia
(3) FKIP Universitas Bengkulu, Indonesia

Abstract


The purpose of this study was to determine the phytochemical profile of Zanthoxylum acanthopodium DC plant (Andaliman) and to know the extract part of plant ethanol Z acanthopodium DC as the best anticancer agent. Root basal samples, stem bark, leaves and fruit Z acanthopodium DC as much as 3 Kg, then dried up and smoothed. Fine samples of root, bark, leaves and fruit of Z acanthopodium DC were each weighed 450 gr and each sample was extracted using 5 L ethanol solvent. Root extract, bark, leaves and fruit Z anthoxylum acanthopodium DC obtained from extraction weighed as much as 1 g, then each of them in concentrations of 10, 100, and 1000 ppm. Cytotoxic tested using BSLT method with shrimp larvae of 10 tail, then determined LC50 by using relationship between probit and log of concentration. Each sample of fine roots, bark, leaves and fruit Z acanthropodium DC were weighed 3 grams. Then press the phytochemical profile. Phytochemical profile test results of each sample of acanthopodium DC Z showed the presence of alkaloids, flavonoids, tannins, terpenoids and saponins. The largest alkaloid content is found on the skin of stems and roots (++) with Papaya leaf comparison, the largest flavonoid content of bark (++) skin with peach flower compound, tannin leaf and tanug (+++) seedlings with Avocado seed comparator, and the largest Kandugan saponin on bark (+++) with the comparative Starfruit Wuluh. In the cytotoxic test results, each leaf extract of stem, leaves, roots and Z acanthopodium DC fruit has potential as an anticancer agent because the liberating LC50 in each sample is in the range 30-1000 ppm. The extract part of the stem bark has the greatest potential as an anticancer compared to fruits, leaves and roots. Because bark leaf extract has LC50 so that 57,677 ppm compared to root of 65,313 ppm, leaves of 77, 983 ppm, and fruit of 191,426 ppm. Usually root samples, stem bark, leaves and fruit Z acanthopodium DC can be used as an anticancer because of the content of flavonoids, saponins, alkaloids, tannins, and terpenoids.

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References


Kementrian Kesehatan RI. 2015. Stop Kanker. Jakarta : Pusat Data dan Informasi Kementrian Kesehatan RI. ISBN. 978-602-288-145-2.

Keputusan Mentri Kesehatan Republik Indonesia. 2007. Mengenai Kebijakan Obat Tradisional Nasional. Nomor 381/MENKES/ SK/III/2007.

Wijaya, Cindy A., Muchtaridi M. 2014. Pengobatan kanker melalui metode gen terapi. Jurnal Farmaka. 2014 : 15 (1) : 53-58.

Adelina, R., Rahmi F., Intan S. O., Putri Reno Intan. Ekstrak daun Annona Muricata Linn Sebagai Antiploriferasi Tergadap Sel Hepar Tikus 7,12

Dimetilbenz (a) antracene (DMBA). Jurnal Kefarmasian Indonesia. 2013 : 4 (1) : 1-4.

Media Unika, 2012. Andaliman (Zanthoxylum Acanthopodium DC) Dan Pemanfaatannya, No. 84/ TH. XXV April – Juni 2012 : 123-125.

Chou, T.Su., Hsiu-Hui C., Hsin-Yi P., Meei-jen L.,Tian S. W. Isolation Of Substance With Antiploriferative And Apoptosis-Inducing Activities Against Leukimia Cell From The Leaves Of Zanthoxylum ailanthoides Sieb. &

Zucc. Jurnal Phytomedicine. 2011 : 18 : 344-348

Tensiska., Hanny W., Nuri A. Aktivitas Antioksidan Ekstrak Buah Andaliman (Zanthoxylum acanthopodium DC) dalam Beberapa Siste

Pangan dan Kesetabilan Aktivitas Terhadap Kondisi Suhu dan pH. Jurnal teknol dan industri pagan. 2003 : 14(1) : 29-39

Wijaya, C.H. Telaah Ringkas Rempah-rempah Tradisional. Andaliman, Rempah Tradisional Sumatera Utara dengan Aktivitas Antioksidan

dan Antimikroba. Buletin Teknologi dan Industri Pangan. Jurnal Bul.teknol dan industri pangan. 1999: 10 (2) : 51-69

Vivi L., Sumali W., Broto S.K. Brine Shrimp Lethality Test (Bslt) Dari Berbagai Fraksi Ekstrak Daging Buah Dan Kulit Biji Mahkota D

E-A (Phaleria Macrocarpa). Jurnal Bul. Panel. Kesehatan. 2006 : 34 (3) : 111-117

Ratnawati, D. Preliminary Test of Determination of Alkaloid and Steroid Compounds and BioassaYon Some Vegetable Plant Extract. Jurnal Gradien 2011 : 7 (2) : 692-6

Agustina, W., Nurhamidah., Dewi, H. Skrining Fitokimia dan aktivitas antioksidan beberapa fraksi dari kulit batang jarak (Ricinnus communis

L). Alotrop 2017:1(2):117-122

Adfa, M. Survey Etnobotani, Studi Senyawa Flavonoid Dan Uji Brine Shirmp Beberapa Tumbuhan Obat Tradisional Suku Serawai Di

Provinsi Bengkulu. Jurnal Gradien. 2005 : 1 (1) : 43-50

Marlina., Meiske S., Audy D. Analisa Senyawa Metabolit Sekunder dan Uji Sitotoksik Ekstrak Etanol Biji Buah Alpokat (Parcea americana Mill). Jurnal Unstrat Online 1 (1) : 24-28

Ikalinus, R., Sri K.Y., Ni L.E.S. Skrining Fitokimia

Ekstrak Etanol Kulit Batang Kelor (Moringa

oleifera). Jurnal Indonesia Medicus Veterinus.

4(1) : 71-79

Fahrunida., Rarasteoti. P. Kandungan Saponin, Buah, Daun Dan Tangkai Daun (Averrhoa balimbi L). Jurnal ilmiah sains 2015 : 1(1):220-204.

Arundina, M ., Theresia I. B., Muhamma L., Retno I. Identifikasi Kromatografi Lapis Tipis Sudamala (Ertemisia vulgaris L) Maj Ked Gi

Ind 1 (2) : 167-171

Pangestu N.S., Nurhamida., Elvinawati. Aktivitas antioksidan dan antibakteri ekstrak daun Jatropha gossypifolia L. Jurnal Alotrop. 2017:

(1) : 15-19.

Zuhra, C. F., J. Br. Tarigan., H. Sitohang. Aktivitas Antioksidan Senyawa Flavonoid dan Daun katuk (Saouropus androgunus (L) Merr). Jurnal

Biologi Sumatera. 2008 : 3(1) : 7-10

Yulia , Mega., Devahimer H.R. Uji Aktivitas Sitotoksik Ekstrak Etanol dari Variasi Teh Daun Sirsak (Annona muricata linn) Terhadap Larva

Udang (Artemia salina leach). Jurnal scientia. 2016 : 6 (1) : 13-16.

Simanjuntak, P., Samsoedin R., Parwati T., Widayanti. Uji Toksisitas Ekstrak Tumbuhan Sukun Anno naceae. Jurnal Biologi Indonesia.

: 3(1) : 5-6.

Meyer, B. N., Ferrigni, N. R., Putnarn, J. E., Jacobsen, L. B., Nichols, D. E. and Mc Laughlin, J. L. Brine Shrimp: A Convenient General Bioassay for Active Plant Constituents. Planta Medica. Jurnal Of Medical Plant

Research. 1982 : 45 (1) : 31-34.

Priyanto, B., Lilian. Farmokoterapi &Terminologi Medis. Jawa Barat : Lembaga Studi dan Konsultasi Farmokologi. ISBN : 979-172-022-9.

Siregar, Syofian. 2011. Statistik Deskriptif untuk Penelitian. Rajawali Pers. Jakarta. ISBN 978-979-769-310-7.


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