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Abstract

This study aims to determine the level of antioxidant activity of Sikkam bark extract (Bischofia javanica Blume) and its potential as an anticancer agent. This research was conducted in November 2018 - April 2019 in the Laboratory of Chemical Education Guidance and Counseling and Basic Science Laboratory, University of Bengkulu. The sample in this study was obtained from Gunung Mariah village in Simalungun district, North Sumatra. The method used in this study was extraction using maceration, liquid-liquid fractionation, phytochemical test, DPPH (Diphenylpicrylhiydrazyl) test and BSLT test (Brine Shrimp Lethality Test). Extraction maceration using ethanol 96%, fractionation using a solvent n-hexane and ethyl acetate, phytochemical tests using Mayer's reagent, Lieberman-Burchard, FeCl3 1% and H2SO4 2N, antioxidant test using DPPH (1,1-diphenyl-2-picrylhydrazyl) and cytotoxic tests using shrimp Artemia salina Leach larvae. The results of the study on maceration of 800 grams of Sikkam bark obtained a crude extract of 53.6 grams (6.7%). Fractionation from 10 grams of crude extract obtained 7.24 gram ethanol fraction (72.4%), ethyl acetate fraction 2.14 gram (21.4%) and n-Hexane fraction 0.39 gram (3.9%). Phytochemical test results obtained the content of alkaloids, flavonoids, tannins, saponins and terpenoids with the highest yield on tannin compounds. The antioxidant activity test results obtained IC50 values in crude extracts of 20.94 ppm, ethanol fraction 36.29 ppm, ethyl acetate fraction 83.28 ppm and n-Hexane fraction 39.13 ppm which showed that crude extract, ethanol fraction, n-Hexane fraction were categorized as very strong antioxidants and Ethyl acetate fraction is categorized as a strong antioxidant. The cytotoxic test results using crude extract obtained an LC50 value of 54,827 ppm which showed that Sikkam bark extract was toxic so that it was potential as an anticancer agent.

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How to Cite
Manurung, D. P., Sundaryono, A., & Amir, H. (2020). PENENTUAN POTENSI EKSTRAK KULIT BATANG TUMBUHAN SIKKAM (Bischofia javanica Blume) SEBAGAI ANTIOKSIDAN DENGAN METODE DPPH DAN SITOTOKSIK DENGAN METODE BSLT. ALOTROP, 4(1). https://doi.org/10.33369/atp.v4i1.13715
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References

  1. Parthasarathy,S., R. Jayaraman., N. Palani and P.Kuppusami. Botanicals InecoFriendly Post Harvest Diseasemanagement. Innovative Farming, 2016 : 1(3): 67-71.
  2. Sukandar, D.,N. Radiastuti., I. Jayanegara, dan A. Hudaya. Karakterisasi Senyawa Aktif Antibakteri Ekstrak Air Bunga Kecombrang (Etlingera Elatior) Sebagai Bahan Pangan Fungsional. Jurnal valensi.
  3. : 2(1) : 333-339.
  4. Andriani, Y, N. M. Ramli, D.Syamsumira. M. Kassima, J.Jaafara, N. Aziza,
  5. L.Marlinaa, N.S Musaa, H.Mohamad. Phytochemical analysis, antioxidant,
  6. antibacterial and cytotoxicity properties of keys and cores part of Pandanus tectorius fruits. Arabian Journal of Chemistry.2015:11(3):1-10
  7. Sangi, M., M. R. J. Runtuwene., H. E. I. Simbala dan V. M. A. Makang. Analisis Fitokimia Tumbuhan Obat Di Kabupaten Minahasa Utara. Chemistry Progress. 2008:1(1):47-53.
  8. Ratnawati, D. Preliminary Test of Determination of Alkaloid and Steroid
  9. Compounds and BioassaY on Some Vegetable Plant Extract. Jurnal Gradien. 2011 : 7 (2) : 692-696.
  10. Alen, Y., Fitria L. A., Yori Y. Analisis Kromatografi Lapis Tipis (KLT) dan
  11. Aktivitas Antihiperurisemia Ekstrak Rebung Schizostachyum brachycladum Kurz (Kurz) pada Mencit Putih Jantan (Thin
  12. layer chromatography (TLC). Jurnal Sains Farmasi dan Klinis. 2017 : 3(2) : 146-152.
  13. Fahrunnida dan Rarastoeti, P. Kandungan Saponin Buah, Daun dan Tangkai Daun Belimbing Wuluh (Averrhoa bilimbi L.).Jurnal Ilmiah Sains. 2015 : 1(1) : 220-224.
  14. Marlinda, M., M.S. Sangi, A.D Wuntu. Analisis Senyawa Metabolit Sekunder dan Uji Toksisitas Ekstrak Etanol Biji Buah Alpukat (Persea americana Mill.)Jurnal Mipa Unsrat Online. 2012 : 1 (1) : 24-28.
  15. Yulia, Mega., D.V Rosi. Uji Aktivitas Sitotoksik Ekstrak Etanol dari Variasi Teh Daun Sirsak (Annora muricata linn) Terhadap Larva Udang (Artemia salina Leach). Jurnal Permata Indonesia, 2014 :5(2):1-7.
  16. Yusriana, C.S., Budi, dan T, Dewi. Uji Daya Hambat Infusa Daun Nangka
  17. (Artocarpus Heterophyllus) Terhadap Pertumbuhan Bakteri Staphylococcus Aureus. Jurnal pertama Indonesia, 2014 : 5(2):1-7.
  18. Angelina, M., M.Turnip., S. Khotimah. Uji Aktivitas Antibakteri Ekstrak Etanol Daun Kemangi (Ocinum sanctum L.) Terhadap Pertumbuhan Bakter Escherichia coli dan Staphylococcus aureus. Jurnal Protobiont.
  19. : 4(1):184-189.
  20. Sarfina, J., Nurhamidah., D, Handayani. Uji Aktivitas Antioksidan dan Antibakteri Ekstrak Daun Ricinus Communis L(Jarak Kepyar). Jurnal Pendidikan Ilmu Kimia. 2017 : 1(1):66-70.
  21. Vitalia, N., Ahmad Najib, A.R. Ahmad. Uji Toksisitas Ekstrak Daun Pletekan (Ruellia tuberosa L.) Dengan Menggunakan Metode
  22. Brine Shrimp Lethality Test (BSLT). Jurnal Fitofarmaka Indonesia. 2016 : 1 (3): 124-129.
  23. Asmiyarti, N.I., Muhamad A.W. Uji Aktivitas Antioksidan Metode DPPH dan
  24. Uji Steud). Jurnal Kimia Khatulistiwa, 2014 : 3 (4) :48-62
  25. Afriani, N., Nora, I., Dan Andi, H.I,. Uji Toksisitas Ekstrak Akar Mentawa
  26. (Artocarpus anisophyllus) Terhadap Larva Artemia salina Leach. Jurnal Kimia Khatulistiwa. 2016 : 5(1) : 58-64.
  27. Haryati,N.A., Chairul,S., Erwin. Uji Toksisitas Dan Aktivitas Antibakteri
  28. Ekstrak Daun Merah Tanaman Pucuk Merah (Syzygium myrtifolium Walp.)
  29. Terhadap Bakteri Staphylococcus aureus dan Escherichia coli. Jurnal Kimia
  30. Mulawarman .2015 :13 ( 1) : 35-40.