ISOLASI BAKTERI DARI TANAH TEMPAT PEMBANGAN AKHIR (TPA) AIR SEBAKUL SEBAGAI AGEN BIODEGRADASI LIMBAH PLASTIK POLYETHYLENE

Desy Purnama Sari(1), Hermansyah Amir(2), Rina Elvia(3),
(1) FKIP Universitas Bengkulu, Indonesia
(2) FKIP Universitas Bengkulu, Indonesia
(3) FKIP Universitas Bengkulu, Indonesia

Abstract


This study aims to obtain the bacteria of Pseudomonas from the land of TPA Air Sebakul Bengkulu City as a plastic degrading agent and measure the ability of bacteria to degrade Low Density Polyethylene (LDPE) and Oxium plastic. The research was conducted from February to August 2019, at the Laboratory of Biology and Chemistry Learning, Faculty of Teacher Training and Education, University of Bengkulu. Air Sebakul landfill samples were taken at the coordinate point 3o49 '27.8 "S 102o20 '48.4" E. Isolation of plastic degrading bacteria using selective media King's B Agar added with 2% Polyethylen Glycol (PEG) to test the ability to develop bacterial isolates in plastic-based media. The steps of this research are bacterial isolation, bacterial purification and bacterial selection, macroscopic and microscopic identification of bacteria (Gram staining) and plastic biodegradation test with Mineral Salt Agar (MSM) media. Determination of the% weight loss of LDPE and oxium plastics in the biodegradation process was carried out for 30 days with time variations of 10, 20 and 30 days. The results of bacterial isolation based on morphological characteristics and gram staining test of P-1 bacterial isolate have similarities with Pseudomonas aeruginosa. So that the P-1 bacterial isolate is thought to be a Pseudomonas aeruginosa bacterium. The bidoegradation of LDPE and oxium plastics with isolates of P-1 bacteria for 10, 20 and 30 days respectively was able to degrade oxium plastics by 2.43, 5.17 and 9.86% while LDPE plastics by 1.13, 2 and 1 , 17%.


Full Text:

PDF

References


Asmita, K., Tanwar. S., dan Shanbhag T. 2015. Isolation of Plastic Degrading Micro-organism from Soil Samples Collected at Various Location in Munbai, India. International Research Journal of Environment Sciences. 4(3): 77-85.

Steensgaard, Ida M., Krystian S., Sinjay. R., Nanna B. H., Alessio. B., Steffen. F. H. 2017. From Macro To Microplastic Analysis Of UE Regulation Along The Life Cicle Of Plastics Bags. Environmental Pollution. 224 : 289-299.

Wilkes, R.A., L. Aristilde., 2017. Degradation And Metabolism Of Syntehtic Plastics and Associated Product By Pseudomonas sp : Capabilites and Challenges. Jurnal Of Applied Microbiology. 123: 582-593

Al-Saleem, S.M., A.Y Al-Naseer., M. H Behbehani., H. H Sultan., H. J Karam., M.H Al-Wadi., A. T Al-Dhafeeri., M. Al-Foudaree. 2019. Thermal Response and Degressive Reaction Study of Oxo-Biodegradabel plastic Product Exposed to Various Degradation Media. Int. Journal Polymer Science. (2): 1-15

Nourollahi. Asieh., Samaneh. S. K., Mehdi. M., Gilda. E., Mahbubeh Shiranian. 2018. Isolation and Identification Of Low Density Polyethylene (Biodegrading Bacteria From Weste Landfill in Yazd. International Jurnal Of Environment Studies. 76 (2): 1-14.

Ahmed. T., Muhammad S., Farrukh A., Ijaz R., Asad A.S., Muhammad N., Amir H., Natasha M., Irfan M., Sher M. 2018. Biodegradation of Plastics : Current Scenario and Future Prospect For Environmental Safety. Environtmental Science and Pollutin Research. 25 : 7287-7298.

Surono, U.B., dan Ismanto. 2016. Pengolahan Sampah Plastik PP, PET dan PE Menjadi Bahan Bakar Minyak dan Karakteristiknya. Jurnal Mekanika dan Sistem Terma (JMST). 1(1) : 32-37.

Riandi, M. I., R. Kawuri., S. K, Sudirga. 2017. Potensi Bakteri Pseudomonas sp. dan Orchobactrum sp. Yang Diisolasi Dari Berbagai Sampel Tanah Dalam Mendegradasi Limbah Polimer Plastik Berbahan Dasar High Density Polyethylene (HDPE) dan Low Density Polyethylene (LDPE). Jurnal Simbiosis. 5 (2) : 58-63

McBirney, Samanta. E., Kristy Trinh., Annie. W. Bringer., Andrea. M. Armani. 2016. Wavelength-Normalized Spectroscopic Analysis of Staphylococcus aureus and Pseudomonas aeruginosa Growth Rates. Biomedical Optic Express. 7 (10) : 4034-4042.

Yulvizar, Cut. 2013. Isolasi dan Identifikasi Bakteri Probiotik pada Rastrelliger sp. Biospecies. 6(2): 1-7.

Viana, A. A.G., B. T. M de Oliveira., T. G. Cavalcanti., K. A. de Sausa., E. A. de Moura Mendocana., I. P. G. do Amaeral., U. Vasconcelos. 2018. Corellation between Pyocyanin Production and Hydrocarbonoclastic Activity In Nine Strains of Pseudomonas aeruginosa. IJAERS. 5 (7) : 212-223.

Charkoudian, L. K., J. T. Fitzgerald., Chaitan K., Andrea. C. 2010. In Living Color: Bacterial Pigments as an Untapped Resource in the Classroom and Beyond. PLOS Biology. 8(10): 1 – 6.

Agustien, A., Mifthahul J., Akmal D. 2016. Screening Polyethylene Synthetic Plastic Degrading-Bacteria from Soil. Der Pharmacia Lettre. 8 (7): 183 – 187

Singh, Shivangi., Upma.D., AK. B., Sachin. G., Vikas. G., Sonika Jamwal. 2017. Morpho-Cultural and Biochemical Identification Of Pseudomonas sp. Isolated From The Rizhosphere Of Different Vegetable Crops and Study Its Efficacy On Solanum Melongena (Brinjal). Journal of Pharmacognosy and Phytochemistry. 6 (2) : 22-28.

Kumar, Vijay., Simranjeet Singh., Joginder Singh., Niraj Upadhyay. 2015. Potential of Plant Growth Promoting Traits by Bacteria Isolated from Heavy Metal Contaminated Soil. Bull Environ Contam Toxical. 94 (6) : 807-814

Mona, Sadasivam., Priya. T., Krishna. K., Subramanian. C.S., Karuppan. P. 2016. Production and Biologycal Activity Of Fluoroscent Secondary Metabolitme From Selected Pseudomonas spp. Indian Jurnal Of Applied Microbiology. 19 (2) : 1-10

Rahayu, Susi Afrianti dan Muhammad Hidayat Gumilar. 2017. Uji Cemaran Air Minum Masyarakat Sekitar Margahayu Raya Bandung Dengan Identifikasi Bakteri Escherichia coli. IJPST. 4 (2) : 50-56

Manasa. K., R.Subhash Reddy., S. Triveni. 2017. Isolation and Characterisation of Pseudomonas fluorescen Isolates From Different Rhizopere Soil of Telanga. Journal of Pharmacognosy and Phytochemistry. 6 (3) : 224-229.

Marjayandari, L., Maya Shovitri. 2015. Potensi Bakteri Bacillus sp. Dalam Mendegradasi Plastik. Jurnal Sains dan Seni ITS. 4 (2) : 59-62

Emadian, S. Mehdi., T. T. Onay., B. Demirel. 2017. Biodegradation of Bioplastics in Natural Environments. Waste Management. 59 : 526-236

Vimala, P.P., L. Mathew. 2016. Biodegradation of Polyethylene Using Bacillus subtilis. Procedia Technology. 24 : 232-239

Kyaw, B. M., Ravi. C., Meena. K. S., Chu. S. L., Kishore. R., Sakharkar. 2012. Biodegradation of Low Density Polyethylen (LDPE) By Pseudomonas species. India Jurnal Microbiol. 52 (3): 411-419

Tandio, Sugianto., E. Suryana., J. Mogck., D. Melander. 2017. Plastics Reagent and Process. Google Patents. Paten AS : 9.617.397

Sachan, S.,Iqbal M.S., Singh A. 2018. Extacellular Lipase From Pseudomonas aeuginosa JCM5962(T) : Isolation, Identification and Characterization. International Microbiology. 21 (4) : 197-205


Article Metrics

 Abstract Views : 0 times
 PDF Downloaded : 0 times

Refbacks

  • There are currently no refbacks.


Alotrop indexed by :

           

ALOTROP

Program Studi Pendidikan Kimia Gedung Dekanat FKIP UNIB Lantai 2
Jl WR Supratman Kandang Limun Bengkulu
Telp/Fax (0736) 21170
e-mail : alotrop.fkip@unib.ac.id

View My Stats