AKTIVITAS ANTIOKSIDAN METABOLIT SEKUNDER BAKTERI ENDOFIT AKAR TANAMAN Moringa oleifera L (Kelor)

Zeta Kuntari(1), Sumpono Sumpono(2), Nurhamidah Nurhamidah(3),
(1) Universitas Bengkulu, Indonesia
(2) Universitas Bengkulu, Indonesia
(3) Universitas Bengkulu, Indonesia

Abstract


[ANTIOXIDANT ACTIVITY OF SECONDARY METABOLITE FROM ENDOFIT BACTERIA OF  Moringa oleifera L (KELOR) ROOTS]  The purpose of this research was aims to isolate and measure the ability of antioxidant activity from secondary metabolites produced by endophytic bacteria that grow in the live tissue root  of Moringa oleifera L. (kelor). Endophytic bacteria were purified and cultured using a solid  Murashige-skoog (MS)  medium for 3 days at room temperature. Secondary metabolites were obtained by centrifugation process at a rate of 3000 rpm for 20 minutes. The bacterial fermentation process  using  Nutrient Broth  (NB) medium for 72 hours with a shaker speed at 170 rpm . The suspension supernatant was extracted with a maceration method using 86% ethyl acetate, followed by vacuum rotary evaporator concentration at 40 ° C. The extract antioxidant activity test  was performed using the DPPH (1,1-diphenyl-2-picrylhydrazyl) method using a UV-Vis spectrophotometer at 517 nm wavelength and ascorbic acid as standard. The result of DPPH test showed that the antioxidant activity of ethyl acetate extract of endophytic bacterial from root of M. oleifera L root has IC50 value at  315, 396 ppm.  Based on these results, it can be concluded that the secondary metabolite extract of endophytic bacterial from M. oleifera L root classified as weak antioxidant (IC50> 250 ppm).


Full Text:

PDF

References


Amir, H, B.G. Murcitro, Uji Microtetra-zolium (MTT) Ekstrak Metanol Daun Phaleriamacrocarpa (Scheff.) Boerl Terhadap Sel Kanker Payudara MCF7 , Alotrop, 2017: 1 (1) : 27-32

Andriani, Y, M.N.Ramli, Desi Fitria Syamsumir, Murni .Nur Islamiah . Kassim, J.Jaafar, J N.A. Aziz, Leni Marlina, N.S. Musa, Habsah Mohamad, Phytochemical Analysis, Antioxidant, Antibacterial and Cytotoxicity Properties Of Keys and Cores Parts Of Pandanus Tectorius Fruits , Arabian Journal of Chemistry. 2015; http://dx.doi.org/ 10.1016 /jarabjc.2015.11.003

Andriani,Y Habsah Mohamad, Murni .Nur.Islamiah Kassim, N.D.Rosnan, Desi Fitria Syamsumir, J. Saidin, Tengku.Sifizul.Tengku. Muhammad, Herman-syah Amir, Evaluation on Hydnophytum formi-carum Tuber from Setiu Wetland (Malaysia) and Muara Rupit (Indonesia) for Antibacterial and Antioxidant activities, and anti-cancer Potency against MCF-7 and HeLa Cells, Journal of Applied Pharmaceutical Science 2017 : 7 (9): 30 -37.

Anwar, F, Latif, S, Asraf, M, Gilani, A,H. Moringa oleifera: A food Plant with Multiple Medicinal Uses, Phyto- ther. 2007.: Res 21: 17-25

Pranoto, Eko, Gilang Fauzi, Hingdri. Isolasi dan Karakterisasi Bakteri Endofit Pada Tanaman Teh (Camelia sinen-sis(L)O. Kuntze) Produktif dan Belum Menghasilkan Klon GMB 7 Dataran Tinggi, Biospecies. 2014: 7(1): 1-7

Nursanty, R, dan Suhartono.Isolasi Ka-rakterisasi dan Uji Antimikroba Bakteri Endofit Asal Tumbuhan Johar (Cassia siamea Lamk) Jurnal Ilmiah Pendidikan Biologi, Biologi Edukasi. 2012: 4(1): 7-10

Tan RX, Zou WX. Endophytes: A Rich Source of Functional Metabolites, the royal society of chemistry. Nat Prod. 2001. Rep. 18: 448 459

Radji,Maksum.2005.Peranan Bioteknologi dan Mikroba Endofit dalam Pe-ngembangan Obat Herbal. Majalah Ilmu Kefarmasian. 2005: 2(3): 113-126.

Radji, M, Sumiati, A.,Rachmayani, R., Elya B. Isolation of fungal endophytes from Garcinia mangostana and their antibacterial activity. African Journal of Biotechnology. 2011: 10(1): 103-107.

Kumala,Shirly, Shanny,F,dan Wahyu-di, P. Uji Aktivitas Antimikroba Meta-bolit Bioaktif Mikroba Endofitik Tana-man Trengguli (Cassia fistula L.). J Farmasi Indonesia. 2006:3(2):97– 102.

Pratiwi, Sylvia T. 2008. Mikrobio- logi Farmasi. Jakarta : Erlangga

Strobel, G.A. dan Daisy, B. Biorespecting for Microbial Endophytes and Their Natural Products, Microbial Mole-cular Biology Review. 2003: 67 : 491-502.

Mohamad H , Andriani , Y.,Bakar , K.,CC Siang CC, Syamsumir,D.F., Alias,A., and Radzi, S.A.M., Effect of drying method on anti-microbial, anti-oxidant activities and isolation of bioactive compounds from Peperomia pellucida (L) Hbk, Journal of Chemical and Pharmaceutical Research, 2015: 7 (8): 578-584.

Prakash, A., F. Rigelhof dan E. Miller. 2001. Antioxidant Activity. Medallion Laboratorie: Analitycal Progress., 2001: 19 (2): 1-4.

Andriani Y, Muhammad, T.S.T. Mohamad , H., Saidin,J., Syamsumir,D.F. Chew GS and Wahid, M.E.A., Phaleria macrocarpa Boerl. (Thymelaeaceae) Leaves Increase SR-BI Expression and Reduce Cholesterol Levels in Rats Fed a High Cholesterol Diet , Molecules , 2015: 20: 4410-4429.

Jun, M. H. Y., J, Fong.X., Wan, C.S., Yang, C.T., Ho. 2003. Comparison of antioxidant activeties of isoflavones from kudzu root (Puerarua labata O). Journal food science Institute of technologist. 68: 2117-2122.

Andriani Y, Wahid, M.E.A., Muhammad T.S.T. ,and Mohamad , H., AntibacterialL, Radical – Scavenging Activities And Cytotoxicity Pro-perties Of Phaleria macrocarpa (Scheff.) Boerl leaves In HEPG2 Cell Lines International Journal of Pharma-ceutical Science and Research, 2011: 2, (7): 1700-1706.




DOI: https://doi.org/10.33369/atp.v1i2.3483

Article Metrics

 Abstract Views : 49 times
 PDF Downloaded : 252 times

Refbacks

  • There are currently no refbacks.


Copyright (c)



Alotrop indexed by :

           

ALOTROP

Program Studi Pendidikan Kimia Gedung Dekanat FKIP UNIB Lantai 2
Jl WR Supratman Kandang Limun Bengkulu
Telp/Fax (0736) 21170
e-mail : alotrop.fkip@unib.ac.id

View My Stats