Main Article Content
Abstract
[ANTIOXIDANT ACTIVITY OF SECONDARY METABOLITE FROM ENDOFIT BACTERIA OF Moringa oleifera L (KELOR) ROOTS] The purpose of this research was aims to isolate and measure the ability of antioxidant activity from secondary metabolites produced by endophytic bacteria that grow in the live tissue root of Moringa oleifera L. (kelor). Endophytic bacteria were purified and cultured using a solid Murashige-skoog (MS) medium for 3 days at room temperature. Secondary metabolites were obtained by centrifugation process at a rate of 3000 rpm for 20 minutes. The bacterial fermentation process using Nutrient Broth (NB) medium for 72 hours with a shaker speed at 170 rpm . The suspension supernatant was extracted with a maceration method using 86% ethyl acetate, followed by vacuum rotary evaporator concentration at 40 ° C. The extract antioxidant activity test was performed using the DPPH (1,1-diphenyl-2-picrylhydrazyl) method using a UV-Vis spectrophotometer at 517 nm wavelength and ascorbic acid as standard. The result of DPPH test showed that the antioxidant activity of ethyl acetate extract of endophytic bacterial from root of M. oleifera L root has IC50 value at 315, 396 ppm. Based on these results, it can be concluded that the secondary metabolite extract of endophytic bacterial from M. oleifera L root classified as weak antioxidant (IC50> 250 ppm).
Article Details
Authors who publish with this journal agree with the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
- This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
References
- Amir, H, B.G. Murcitro, Uji Microtetra-zolium (MTT) Ekstrak Metanol Daun Phaleriamacrocarpa (Scheff.) Boerl Terhadap Sel Kanker Payudara MCF7 , Alotrop, 2017: 1 (1) : 27-32
- Andriani, Y, M.N.Ramli, Desi Fitria Syamsumir, Murni .Nur Islamiah . Kassim, J.Jaafar, J N.A. Aziz, Leni Marlina, N.S. Musa, Habsah Mohamad, Phytochemical Analysis, Antioxidant, Antibacterial and Cytotoxicity Properties Of Keys and Cores Parts Of Pandanus Tectorius Fruits , Arabian Journal of Chemistry. 2015; http://dx.doi.org/ 10.1016 /jarabjc.2015.11.003
- Andriani,Y Habsah Mohamad, Murni .Nur.Islamiah Kassim, N.D.Rosnan, Desi Fitria Syamsumir, J. Saidin, Tengku.Sifizul.Tengku. Muhammad, Herman-syah Amir, Evaluation on Hydnophytum formi-carum Tuber from Setiu Wetland (Malaysia) and Muara Rupit (Indonesia) for Antibacterial and Antioxidant activities, and anti-cancer Potency against MCF-7 and HeLa Cells, Journal of Applied Pharmaceutical Science 2017 : 7 (9): 30 -37.
- Anwar, F, Latif, S, Asraf, M, Gilani, A,H. Moringa oleifera: A food Plant with Multiple Medicinal Uses, Phyto- ther. 2007.: Res 21: 17-25
- Pranoto, Eko, Gilang Fauzi, Hingdri. Isolasi dan Karakterisasi Bakteri Endofit Pada Tanaman Teh (Camelia sinen-sis(L)O. Kuntze) Produktif dan Belum Menghasilkan Klon GMB 7 Dataran Tinggi, Biospecies. 2014: 7(1): 1-7
- Nursanty, R, dan Suhartono.Isolasi Ka-rakterisasi dan Uji Antimikroba Bakteri Endofit Asal Tumbuhan Johar (Cassia siamea Lamk) Jurnal Ilmiah Pendidikan Biologi, Biologi Edukasi. 2012: 4(1): 7-10
- Tan RX, Zou WX. Endophytes: A Rich Source of Functional Metabolites, the royal society of chemistry. Nat Prod. 2001. Rep. 18: 448 459
- Radji,Maksum.2005.Peranan Bioteknologi dan Mikroba Endofit dalam Pe-ngembangan Obat Herbal. Majalah Ilmu Kefarmasian. 2005: 2(3): 113-126.
- Radji, M, Sumiati, A.,Rachmayani, R., Elya B. Isolation of fungal endophytes from Garcinia mangostana and their antibacterial activity. African Journal of Biotechnology. 2011: 10(1): 103-107.
- Kumala,Shirly, Shanny,F,dan Wahyu-di, P. Uji Aktivitas Antimikroba Meta-bolit Bioaktif Mikroba Endofitik Tana-man Trengguli (Cassia fistula L.). J Farmasi Indonesia. 2006:3(2):97– 102.
- Pratiwi, Sylvia T. 2008. Mikrobio- logi Farmasi. Jakarta : Erlangga
- Strobel, G.A. dan Daisy, B. Biorespecting for Microbial Endophytes and Their Natural Products, Microbial Mole-cular Biology Review. 2003: 67 : 491-502.
- Mohamad H , Andriani , Y.,Bakar , K.,CC Siang CC, Syamsumir,D.F., Alias,A., and Radzi, S.A.M., Effect of drying method on anti-microbial, anti-oxidant activities and isolation of bioactive compounds from Peperomia pellucida (L) Hbk, Journal of Chemical and Pharmaceutical Research, 2015: 7 (8): 578-584.
- Prakash, A., F. Rigelhof dan E. Miller. 2001. Antioxidant Activity. Medallion Laboratorie: Analitycal Progress., 2001: 19 (2): 1-4.
- Andriani Y, Muhammad, T.S.T. Mohamad , H., Saidin,J., Syamsumir,D.F. Chew GS and Wahid, M.E.A., Phaleria macrocarpa Boerl. (Thymelaeaceae) Leaves Increase SR-BI Expression and Reduce Cholesterol Levels in Rats Fed a High Cholesterol Diet , Molecules , 2015: 20: 4410-4429.
- Jun, M. H. Y., J, Fong.X., Wan, C.S., Yang, C.T., Ho. 2003. Comparison of antioxidant activeties of isoflavones from kudzu root (Puerarua labata O). Journal food science Institute of technologist. 68: 2117-2122.
- Andriani Y, Wahid, M.E.A., Muhammad T.S.T. ,and Mohamad , H., AntibacterialL, Radical – Scavenging Activities And Cytotoxicity Pro-perties Of Phaleria macrocarpa (Scheff.) Boerl leaves In HEPG2 Cell Lines International Journal of Pharma-ceutical Science and Research, 2011: 2, (7): 1700-1706.
References
Amir, H, B.G. Murcitro, Uji Microtetra-zolium (MTT) Ekstrak Metanol Daun Phaleriamacrocarpa (Scheff.) Boerl Terhadap Sel Kanker Payudara MCF7 , Alotrop, 2017: 1 (1) : 27-32
Andriani, Y, M.N.Ramli, Desi Fitria Syamsumir, Murni .Nur Islamiah . Kassim, J.Jaafar, J N.A. Aziz, Leni Marlina, N.S. Musa, Habsah Mohamad, Phytochemical Analysis, Antioxidant, Antibacterial and Cytotoxicity Properties Of Keys and Cores Parts Of Pandanus Tectorius Fruits , Arabian Journal of Chemistry. 2015; http://dx.doi.org/ 10.1016 /jarabjc.2015.11.003
Andriani,Y Habsah Mohamad, Murni .Nur.Islamiah Kassim, N.D.Rosnan, Desi Fitria Syamsumir, J. Saidin, Tengku.Sifizul.Tengku. Muhammad, Herman-syah Amir, Evaluation on Hydnophytum formi-carum Tuber from Setiu Wetland (Malaysia) and Muara Rupit (Indonesia) for Antibacterial and Antioxidant activities, and anti-cancer Potency against MCF-7 and HeLa Cells, Journal of Applied Pharmaceutical Science 2017 : 7 (9): 30 -37.
Anwar, F, Latif, S, Asraf, M, Gilani, A,H. Moringa oleifera: A food Plant with Multiple Medicinal Uses, Phyto- ther. 2007.: Res 21: 17-25
Pranoto, Eko, Gilang Fauzi, Hingdri. Isolasi dan Karakterisasi Bakteri Endofit Pada Tanaman Teh (Camelia sinen-sis(L)O. Kuntze) Produktif dan Belum Menghasilkan Klon GMB 7 Dataran Tinggi, Biospecies. 2014: 7(1): 1-7
Nursanty, R, dan Suhartono.Isolasi Ka-rakterisasi dan Uji Antimikroba Bakteri Endofit Asal Tumbuhan Johar (Cassia siamea Lamk) Jurnal Ilmiah Pendidikan Biologi, Biologi Edukasi. 2012: 4(1): 7-10
Tan RX, Zou WX. Endophytes: A Rich Source of Functional Metabolites, the royal society of chemistry. Nat Prod. 2001. Rep. 18: 448 459
Radji,Maksum.2005.Peranan Bioteknologi dan Mikroba Endofit dalam Pe-ngembangan Obat Herbal. Majalah Ilmu Kefarmasian. 2005: 2(3): 113-126.
Radji, M, Sumiati, A.,Rachmayani, R., Elya B. Isolation of fungal endophytes from Garcinia mangostana and their antibacterial activity. African Journal of Biotechnology. 2011: 10(1): 103-107.
Kumala,Shirly, Shanny,F,dan Wahyu-di, P. Uji Aktivitas Antimikroba Meta-bolit Bioaktif Mikroba Endofitik Tana-man Trengguli (Cassia fistula L.). J Farmasi Indonesia. 2006:3(2):97– 102.
Pratiwi, Sylvia T. 2008. Mikrobio- logi Farmasi. Jakarta : Erlangga
Strobel, G.A. dan Daisy, B. Biorespecting for Microbial Endophytes and Their Natural Products, Microbial Mole-cular Biology Review. 2003: 67 : 491-502.
Mohamad H , Andriani , Y.,Bakar , K.,CC Siang CC, Syamsumir,D.F., Alias,A., and Radzi, S.A.M., Effect of drying method on anti-microbial, anti-oxidant activities and isolation of bioactive compounds from Peperomia pellucida (L) Hbk, Journal of Chemical and Pharmaceutical Research, 2015: 7 (8): 578-584.
Prakash, A., F. Rigelhof dan E. Miller. 2001. Antioxidant Activity. Medallion Laboratorie: Analitycal Progress., 2001: 19 (2): 1-4.
Andriani Y, Muhammad, T.S.T. Mohamad , H., Saidin,J., Syamsumir,D.F. Chew GS and Wahid, M.E.A., Phaleria macrocarpa Boerl. (Thymelaeaceae) Leaves Increase SR-BI Expression and Reduce Cholesterol Levels in Rats Fed a High Cholesterol Diet , Molecules , 2015: 20: 4410-4429.
Jun, M. H. Y., J, Fong.X., Wan, C.S., Yang, C.T., Ho. 2003. Comparison of antioxidant activeties of isoflavones from kudzu root (Puerarua labata O). Journal food science Institute of technologist. 68: 2117-2122.
Andriani Y, Wahid, M.E.A., Muhammad T.S.T. ,and Mohamad , H., AntibacterialL, Radical – Scavenging Activities And Cytotoxicity Pro-perties Of Phaleria macrocarpa (Scheff.) Boerl leaves In HEPG2 Cell Lines International Journal of Pharma-ceutical Science and Research, 2011: 2, (7): 1700-1706.