ANTIOXIDANT AND CYTOTOXICITY PROPERTIES OF ETHYL ACETATE FRACTIONS OF Pandanus tectorius FRUIT AGAINST HELA CELL LINES

Noor Suryani Musa(1), Nadia Madiha Ramli(2), Jasnizat Saidin(3), Yosie Andriani(4),
(1) Institute of Marine Biotechnology, Universiti Malaysia Terengganu, Kuala Nerus 20130, Terengganu, Malaysia, Malaysia
(2) School of Marine and Environmental Sciences, Universiti Malaysia Terengganu, Kuala Nerus 21030, Terengganu, Malaysia, Malaysia
(3) Institute of Marine Biotechnology, Universiti Malaysia Terengganu, Kuala Nerus 20130, Terengganu, Malaysia, Malaysia
(4) School of Marine and Environmental Sciences, Universiti Malaysia Terengganu, Kuala Nerus 21030, Terengganu, Malaysia, Malaysia

Abstract


Antioxidant agents play an important role in inhibiting free radical molecules activity thus preventing the cancer disease. This current study aimed to evaluate antioxidant and cytotoxicityproperties of ethyl acetate fractions of Pandanustectorius fruit extract on HeLa cell lines. The radical scavenging activity was measured by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. The cytotoxicity was measured by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay against human HeLa cell lines. The result showed that all ethyl acetate fractions of P. tectoriusfruit had high antioxidant property. All of seven fractions of the ethyl acetate extract were observed based on the IC50 value and had high DPPH free radical scavenging activity with the IC50 value range from 0.3 to 2.4 mg/ml compare to positive control,quercetin(IC50= 0.2 mg/ml). Based on the DPPH free radical scavenging activity (antioxidant property), three of seven fractions were chosen for the MTT assay to analyze their cytotoxic activity as anticancer potential agent against HeLa cell lines.From MTT result, one of tree fractions were showed cytotoxicity activity against HeLa cell lines with the IC50value is 12 µg/ml (less than 30 µg/ml), while anothertwo fractions was found not cytotoxic against HeLa cell lines.It is clearly indicated that P. tectoriusfruit has a very good potential as antioxidant and anticancer agent.


Full Text:

PDF

References


WHO (2015). Cancer: The Problem. World Health Organization.

Pezzuto JM. Plant–derived anticancer agents. Biochemical Pharmacology. 1997:53:121-133.

Wu J, Wu, Yang BB. Anticancer activity of Hemsleyaamabilis extract. LifeScience. 2002: 71: 2161-2170.

Zhang X, Guo P, Sun G, Chen S, Yang M, Wu H, Xu X. Phenolic compounds and flavonoids from the fruits of Pandanus tectorius Soland. Journal of Medicinal Plants Research. 2012:6(13):2622-2626.

Huang WY, Cai Y, Zhang Y. Natural phenolic compounds from medical herbs and dietary plants: potential use for cancer prevention, nutrition and cancer. 2009:62(1): 1-20.

Johnson IT, Williamson G, Musk SR. Anti-carcinogenic factors in plant foods: a new class of nutrients. Nutrition Reviews. 1994: 7: 175-204.

Abrahim NN, Kanthimati MS, Abdul Aziz A. Piper betle shows antioxidant activities, inhibits MCF-7 cell proliferation and increases activities of catalase ad Superoxide dismutase. BMC complementary and alternative medicine. 2012:12: 220.

Kanski J, Aksenova M, Stoyanova A, Butterfield DA. Ferulic acid antioxidant pro-tecttion against hydroxyl and peroxyl radical oxidation in synaptosomal and neuronal cell culture system In Vitro: Structure-activity Studies. Journal of Nutritional Biochemistry. 2002:13: 273-281.

Lee JY, Hwang WI, Lima ST. Antioxidant and anticancer activities of organic extracts from Platycodon grandiflorum A. De Candolle roots. Journal of Ethnopharmacology. 2004: 93(5): 409-415.

Hu FL, Lu RL, Huang B, Ming L.Free Radical scavenging activity of extracts prepared from fresh leaves of selected Chinese medicinal plants. Fitoterapia. 2004: 75(1):14-23.

Duan XJ, Zhang WW, Li, XL, Wang BG. Evaluation of antioxidant property ofextract and fractions obtained from a red alga, Polysiphoniaur ceolata. Science Direct. 2004:95(1):37-43.

Riaz, T., Abbasi, M.A., Aziz,U.R., Shahzadi, T., Ajaib,M and Khan,M. Phytochemical Screening Free Radical Scavenging, Antioxidant Activity and Phenolic Contect of Dodonaca Viscose Jacq. Journal of Serbian Chemical Society. 2012: 77(4):423-435.

Praveen KR, Awang B. Antioxidant Activity, Total phenolic and flavonoid content of Morindaci trifolia Fruit extracts from various extraction processes. Journal of Engineering Science and Technology. 2007: 2(1): 70-80.

Hermien N, Mulyadi T, Nanik SA, Ami SJS. Antioxidant activities of extracts of Trengguli stem bark (Cassia fistula L.) International Journal of Applied Science. 2012:12(4): 85-89.

Andriani Y, Effendy MAW, Habsah M, Sifzizul TMT. Antibacterial, radical-scavenging activities andcytotoxicity pro-perties of Phaleria macrocarpa (Scheff.) Boerl leaves in HepG2 cell lines. International Journal of Pharmaceutical Sciences and Research. 2011:2(7): 1700-1706.

Andriani Y, Ramli NM, Syamsumir DF, Kassim MNI, Jaafar J., N.A.Azis, Marlina, L., Musa NS, Mohamad H., Phytochemical analysis, antioxidant, antibacterial and cytotoxicity Activities of keys and cores part of Pandanus tectorius fruits. Arabian Journal of Chem. 2015.http://doi.org/ 10.1016/ j.arabjc. 2015. 11. 003.

Milugo TK, Omosa LK, Ochanda JO, Owuor BO, Wamunyokoli FA, Oyugi JO, et al. Antagonistic effect of alkaloids and saponins on bioactivity in the quinine tree (Rauvolfia caffra sond.): Further evidence to support biotechnology in traditional medicinal plants. BMC Complement Alternative Meddicine. 2013: 13: 285.




DOI: https://doi.org/10.33369/atp.v1i2.3515

Article Metrics

 Abstract Views : 54 times
 PDF Downloaded : 32 times

Refbacks

  • There are currently no refbacks.


Copyright (c)



Alotrop indexed by :

           

ALOTROP

Program Studi Pendidikan Kimia Gedung Dekanat FKIP UNIB Lantai 2
Jl WR Supratman Kandang Limun Bengkulu
Telp/Fax (0736) 21170
e-mail : alotrop.fkip@unib.ac.id

View My Stats