AKTIVITAS ANTIOKSIDAN DAN ANTIBAKTERI EKSTRAK DAUN Strobilanthes crispus Bl (Keji Beling) ) TERHADAP Staphylococcus aureus DAN Escherichia coli

Sukaina Adibi(1), Hendry Nordan(2), Septri Nurjaya Ningsih(3), Moga Kurnia(4), Evando Evando(5), Salastri Rohiat(6),
(1) Universitas Bengkulu, Indonesia
(2) Universitas Bengkulu, Indonesia
(3) Universitas Bengkulu, Indonesia
(4) Universitas Bengkulu, Indonesia
(5) Universitas Bengkulu, Indonesia
(6) Universitas Bengkulu, Indonesia

Abstract


[ANTIXOXIDANT AND ANTIBACTERIA ACTIVITIES OF Strobilanthes crispus Bl (Keji Beling) EXTRACT AGAINST Stapylococcus Aureus AND Escherichia coli] Strobilanthes crispus Bl (Keji Beling)  is one of herbs that has been used for the treatment of several types of diseases such as kidney stones, gall stones, diabetes, cholesterol, tumors and others. The purpose of this study was to determine the value of antioxidant activity (IC50) and antibacterial activity of ethanol extract of leaf vermicelli against Staphylococcus aureus and Escherichia coli bacteria. Antioxidant test was performed using DPPH method and vitamin C was used as standard. While the antibacterial activity test done by paper disc method. Amphicillin is used as a positive control and aquades as a negative control. Ethanol extract of leaf vermicelli able to counteract free radical of DPPH with IC50 value that is: 102.85 ppm and IC50 value from vitamin C as comparative solution equal to 19.268 ppm. The results of antibacterial inhibition test of ethanol extract of vile leaf on S. aureus bacteria with extract concentration of 20% (0 mm), 40% (2.5 mm), 60% (3.25 mm), and 80% (4.75) were included Weak and 100% (5.75 mm) including Medium, and E.coli antibacterial inhibition in the extract con- tacts of 20% (0 mm), 40% (2 mm), 60% (2.25 mm), 80% (4.25 mm) were weak, and 100% (5.25 mm ) including moderate. So it is known that the extract of ethanol leaves vile at 100% concentration can inhibit the growth of S. aureus bacteria and E. coli bacteria characterized by the formation of the largest clear zone diameter at the concentration. But the strength of its bacteria is still not effective to inhibit the growth of both test bacteria.


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References


Oktarina, D., Sumpono, Rina Elvia. Uji Effektivitas Asap Cair Cangkah Buah Hevea brazilensis Terhadap Aktivitas Bakteria Escherichia coli. Alotrop. 2017:1(1):1-5.

Agustina, W., Sumpono, Rina Elvia. Aktivitas Asap Cair Cangkang Buah Hevea brazilensis sebagai Anti Bakteri Staphylococcus aureus. Alotrop. 2017: 1(1): 6-9.

Melliawati, R. Escherichia coli dalam kehidupan manusia. BioTrends. 2009:4(1): 10-14.

Haposan, E., Suwarman, Redjeki, I.S. Gambaran Pola Kuman pada Bilah Laringoskop di Ruang Operasi Rumah Sakit Dr Hasan Sadikin Bandung. Jurnal Anestesi Perioperatif (JAP). 2016: 4(3):162-169.

Sartika, R.A.D., Yvonne M. Indrawani, Trini Sudiarti. Analisis Mikrobiologi Escherichia coli O157:H7 Pada Hasil Olahan Hewan Sapi dalam Proses Produksinya. MAKARA KESEHATAN. 2005: 9(1):23-38.

Setyawan, A.B., Winarto, Endang.S. Lestari. Pembuktian Ekstrak Daun Kejibeling Dalam Meningkatkan Sistem Imun. Jurnal Kesehatan Masyarakat KEMAS. 2016: 11(2):96-100.

Nurraihana,H. Norfarizan –Hanoon, N.A. Phytochemistry, pharmacology and toxico-logy properties of Strobilanthes crispus. International Food Research Journal. 2013: 20(5):2045-2056.

Andriani, Y., Desy. F. Syamsumir, T.C.Yee, F.S.Harisson, G.M Herng, S.A.Abdullah, C.A. Orosco, A.M.Ali, J. Latip, H. Kikuzaki, H. Mohamad. Biological activities of isolated coumpounds from three edible Malaysian red seaweeds, Gracilaria changii, G. manilaensis and Gracilaria sp. Natural Product Communications. 2016: (8):1117-1120.

Molyneux, P. The Use of The Stable Free Radical Diphenylpicryl-hydrazyl (DPPH) for Estimating Antioxidant Activity. Journal of Science Technology. 2004: 26 (2), 211-219.

Pangestu, N.S, Nurhamidah, Elvinawati. Aktivitas Antioksidan dan Antibakteri Ekstrak Daun Jatropha gossypifolia L Alotrop. 2017:1(1): 15-19.

Bakti, A.A., Liling Triyasmono, M.I. Rizki. Penentuan Kadar Flavonoid Total dan Uji Antioksidan Ekstrak Etanol Daun Kasturi (Mangifera casturi K osterm.) dengan metode DPPH. Jurnal Pharmascience. 2017: 4(1): 102-108.

Sari. M.L., Arfan Abrar, Merint. Isolasi dan Karakterisasi Bakteri Asam Laktat Pada Usus Ayam Broiler. Agripet. 2013: 13(1): 43-48.

Andriani, Y., M.A.W Effendy, TST. Muhamad, H. Mohamad. Antibacterial, radical scavenging activities and cytotoxicity properties of Phaleria macrocarpa (scheff.) Boerl. Leaves in Hepg2 cell lines. International Journal of Pharmaceutical Sciences and Research. 2011: 2(7): 1700-1706.

Davis, W.W., Stout, T.R. Disc Plate Methods of Microbiological Antibiotic Assay. Microbiology. 1991: 22(4) : 659-665.

Prakash, A., Rigelhof, F., Miller, E. Antioxidant Activity. Medallion Laborato-ries : Analithycal Progress. 2001:19(2): 1-4.

Andriani, Y, Habsah Mohamad, M.N.I, Kassim., N.D. Rosnan., D.F. Syamsumir., J.Saidin., T.S.T. Muhammad, Hermansyah Amir. Evaluation on Hydnophytum formicarum Tuber from Setiu Wetland (Malaysia) and Muara Rupit (Indonesia) for Antibacterial and Antioxidant activities and anti-cancer Potency against MCF-7 and HeLa Cell. Journal of Applied Pharmaceutical Science. 2017: 7(9):30-37.

Sudiono.J.,Oka CT., Trisfilha P. The Scientific Base of Murmecodia pendans as Herbal Remedies. Br.J.Med. Medic Res. 2015: 8(3): 230-237.

Mohamad.H, Yosie Andriani, Kamariah Bakar, CC Siang, D.F. Syamsumir, A.Alias., S.A.M. Radzi. Effect of drying method on anti-microbial, anti-oxidant acti-vities and isolation of bioactive coum-pounds from Peperomia pellucida (L) Hbk. Journal of Chemical and Pharmaceutical Research. 2015:7(8): 578-584.




DOI: https://doi.org/10.33369/atp.v1i2.3547

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