UJI AKTIVITAS ANTI BAKTERI EKSTRAK METANOL DAUN BACCAUREA MACROCARPA TERHADAP ESCHERICHIA COLI DAN SALMONELLA TYPHI

Vivi Mardina(1), Fitri Helmalia(2), Fadhliani Fadhliani(3), Lendawati Lendawati(4),
(1) Samudra University, Indonesia
(2) Samudra University, Indonesia
(3) Samudra University, Indonesia
(4) Politeknik Kesehatan Tanjungkaran, Indonesia

Abstract


Antibiotics are drugs that are used to inhibit bacteria production. The long-term use of antibiotics can cause resistance problems. As a consequent it is necessary to find the alternative antibiotics that are derived from plants. The alternative antibiotics are considered safer, relatively cheap and easy to obtain. One of the herbal plants used is Baccaurea macrocarpa. The objective of this study was to identify the secondary metabolites of B.macrocarpa, the effect of methanol extract from B.macrocarpa leaves on the growth of Escherichia coli and Salmonella typhi bacteria and to measure the optimal concentration of the sample to inhibit the growth of E.coli and S.typhi bacteria. The research has been conducted for 3 months at the Universitas Samudra laboratory. The method used was the well method with E.coli and S.typhi bacteria test. Each treatment of the methanol extract of B. macrocarpa leaves with a concentration of 20%, 30% and 40% (g/mL). Positive control was ciprofloxacin and negative control was sterile distilled water. The results obtained the concentration of 40% (g/mL) as the optimal concentration to inhibit the growth of E. coli and S.typhi bacteria with the resulting inhibition zone of 6.3 mm and 5 mm. The results of the study concluded that the B. Macrocarpa leaf extract could be developed as a basic ingredient for antibiotics.


Keywords


Antibacteria activity, Baccaurea macrocarpa, methanol extract

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References


Akhmadi NR., Sumarmiyati. 2015. Eksplorasi dan karakterisasi buah kapul (Baccaurea macrocarpa) di Kabupaten Kutai Barat, Kalimantan Timur. Prosiding seminar nasional masyarakat biodiversitas Indonesia, 1 (4): 923-929

Day DWR, Erwin, Astuti W. 2018. Uji toksisitas dengan metode BSLT ekstrak kasar kulit batang tampoi Baccaurea macrocarpa). Prosiding Seminar Nasional Kimia 2018, Kimia FMIPA Unmul. ISBN 97860250927 No.17.

Fernandez, BAM. 2013. Studi penggunaan antibiotik tanpa resep di Kabupaten Manggarai barat – NTT. Calyptra: Jurnal ilmiah mahasiswa Universitas Surabaya, 2 (2): 1 – 17.

Gunawan, Tatik C, Sobir, Sulistijorini. 2016. Review: fitokimia genus Baccaurea sp. Bioeksperimen 2(2): 96 – 110.

Malangngi LP, Sangi MS, Paendong JJE. 2012. Penentuan kandungan tanin dan uji aktivitas antioksidan ekstrak biji buah alpukat (Persea americana Mill.). Jurnal Mipa Unsrat. 1(1):5-10.

Mardina V dan Yusof F. 2018. Skim Latex Serum as an Alternative Nutrition for Microbial Growth. In: Multifaceted Protocol in Biotechnology. Springer, Singapore.

Mardina V, Al Fajar B, Fitriani (2019) Potency of phytomedicine Sphagneticola trilobata extract as chemopreventive agent on the cases of breast cancer mice 7,12 Dimetilbenz(?)Antrasena induced. Yarsi medical journal, 27(2), 52 – 61.

aMardina V, Ilyas S, Harmawan T, Halimatussakdiah H, Tanjung M (2020) Antioxidant and cytotoxic activities of the ethyl acetate extract of Sphagneticola trilobata (L.) J.F. Pruski on MCF-7 breast cancer cell. J Adv Pharm Technol Res 11 (3): 123 – 127.

bMardina, V., Halimatussakdiah, Harmawan, T., Ilyas, S., Tanjung, ., Aulya, W., Nasution, A. Preliminary phytochemical screening of different solvent extracts of flower and whole plant of Wedelia biflora. IOP Conference Series: Materials Science and Engineering. 3rd Nommensen International Conference on Technology and Engineering 2019 (3rd NICTE) 25–26 July 2019, Nommensen HKBP University, Indonesia.

cMardina, V., Mastura, Hamdani, Sufriadi, E. 2020. Flower of Sphagneticola trilobata (L.) J.F Pruski from Aceh, Indonesia: Antioxidant and Cytotoxic Activity on HeLa Cells. IOP Conf. Series: Materials Science and Engineering 1007 (2020) 012182. 3rd TICATE 2020.

Mardina V, Yusof F, Alam MZ. 2015. Statistical optimization of physicochemical factors for protease production by Bacillus licheniformis on skim latex serum fortified media Journal of Engineering Science and Technology sp issue 6 (1): 42 – 52.

Mohamad R, Widyatuti N, Suradikususmah E, Darusman L.K. 2012. Aktivitas antioksidan, kadar fenol dan flavonoid total dari enam tumbuhan obat Indonesia, Jurnal Trad Met, J, 18: 29 – 34.

Muharni, Fitrya, Farida S. 2017. Uji aktivitas antibakteri ekstrak etanol tanaman obat Suku Musidi Kabupaten Musi Banyuasin, Sumatera Selatan. Jurnal kefarmasian Indonesia, 7 (2): 127 – 135.

Ngajow, Mercy, Jemmy A.V. 2013. Pengaruh antibakteri ekstrak kulit batang matoa (pometia pinnata) terhadap Staphylococcus aureus secara in vitro. Jurnal MIPA UNSTRAT Manado, 3 (2):

Novitaria, Andi, H, A, Lia Destiarti. 2016. Isolasi dan karakteristik golongan senyawa fenolik dari kulit batang tampoi (Baccaurea macrocarpa) dan uji aktivitas antioksidan. Jurnal KK, 5(2): 27 – 32.

Purnamasari, Fita, R., Susilo. Uji daya hambat ekstrak etanol kulit buah terong ungu (Solanum melongena L.) terhadap Staphylococcus aureus dan Escherichia coli. Jurnal inovasi teknik kimia, 3 (1):

Rohdiana D, Dede Zainal Arief DZ, Budiman A. 2013. Aktivitas penghambatan pertumbuhan bakteri Escherichia coli oleh berbagai jenis teh dan seduhannya. Jurnal Penelitian Teh dan Kina, 16 (1) :37 – 44.

Tirtana, E. Idiawati, N. Warsidah, Jayuska A. 2013. Analisa Proksimat, uji Fitokimia dan aktivitas antioksidan pada buah tampoi (Baccaurea Macrocarpa). JKK, 2 (1): 42 – 45.

Tresia. 2016. Uji daya hambat antibakteri ekstrak umbi bawang merah (Allium ascalonicum ) terhadap pertumbuhan bakteri pembentukan karies gigi Streptococus mutans [skripsi] Yogjakarta : Fakultas Keguruan dan Ilmu Pendidikan.

Yunus R, Alimuddin A,A, Ardiningsih P. 2014. Uji aktivitas antibakteri ekstrak kulit buah tampoi (Baccaurea macrocarpa) terhadap bakteri Escherichia coli dan Staphylococcus aureus. Jurnal Kimia Khatulistiwa Vol 3. No 3 : 19-24.




DOI: https://doi.org/10.33369/hayati.v17i1.12879

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Jl. W.R. Supratman, Kandang Limun, Kec. Muara Bangka Hulu, Kota Bengkulu, Bengkulu 38119. FMIPA, Universitas Bengkulu

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