Effect of BAP (6-Benzyl Aminopurine) on In Vitro Shoot Growth of Curcumas

Rustikawati Rustikawati(1), Catur Herison(2), Entang Inoriah(3), Vera Dwisari(4),
(1) Department of Crop Production, Faculty of Agriculture, University of Bengkulu, Indonesia
(2) Department of Crop Production, Faculty of Agriculture, University of Bengkulu, Indonesia
(3) Department of Crop Production, Faculty of Agriculture, University of Bengkulu, Indonesia
(4) Department of Crop Production, Faculty of Agriculture, University of Bengkulu, Indonesia

Abstract


Curcuma sp has been widely investigated for its anti-cancer properties. Conventionally, vegetative propagation needs a long time to produce a large number of planting materials, so that it is necessary to find an alternative approach through in vitro propagation.  The effect of BAP on the in vitro shoot formation of ‘temu putih’ and ‘temu putih’ has been investigated in this study. The experiment was a 4x2 factorial with 5 replications arranged in a completely randomized design.  The first factor was the concentration of BAP i.e. 0, 1.5, 3, and 4.5 ppm.  The second factor was the curcuma species consisting of ‘temu putih’ (Curcuma zedoaria Roch.) and ‘temu mangga’ (Curcuma mangga Val.).  Each experimental unit consisted of 2 in vitro bottles, each of which planted with 1 explant bud.  Analysis of variance was conducted on percentage of live explants, shoot height, number of roots, root length, wet weight, percentage of explants that sprouted, percentage of rooted explants and shoot color.  Mean comparison was performed by the Least Significant Difference (LSD).  The results showed that there was no interaction between BAP concentration and genotype on any variable observed.  The shoot growth of ‘temu putih’ was significantly higher than ‘temu mangga’ in vitro.  The best concentration of BAP for the growth of ‘temu putih’ and ‘temu mangga’ shoots was 1.5 ppm.


Keywords


cytokinine; ‘temu putih’; ‘temu mangga’; in vitro

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References


Abas, F., Lajis, N.H., Shaari, K., Israf, D.A., Stanslas, J., Yusuf, U.K., Raof. S.M. (2005). A labdane diterpene glucoside from the rhizomes of Curcuma mangga. J Nat Prod. 68(7),1090-1093. doi: 10.1021/np0500171.

Ali, A., Naz, S., Siddiqui, F.A. and Iqbal, J. (2008). Rapid clonal multiplication of sugarcane (Saccharum officinarum) through callogenesis and organogenesis. Pakistan Journal of Botany, 40(1),123.

Antoniazzi, D., de-Souza-Ferrari, M.P., Nascimento, A.B., Silveira, F.A., Pio, L.A.S., Pasqual, M. & Magalhães H.M. (2016). Growth regulators, DNA content and anatomy in vitro-cultivated Curcuma longa seedlings. Afric. J. Biotech., 15(32), 1711–1725.

Arniputri, R.B. dan Purnomo, D. (2003). Pengaruh konsentrasi IAA dan BAP terhadap pertumbuhan dan perkembangan tanaman kunir putih (Kaempferia rotunda L.) Secara in vitro. Agrosains, 5(2),48-51.

Aulia, M.I., Rustikawati dan Inoriah. E. (2020). Respon temu putih dan temu mangga dengan pemberian BA dan 2,4-D secara in vitro. Gema Agro 25(2),92~102

Bharalee, R., Das, A.& Kalita. M.C. (2005). In vitro clonal propagation of Curcuma caesia Roxb and Curcuma zedoaria Rosc from rhizome bud explants. J. Plant Biochem. Biotechnol. 14, 61-63 https://doi.org/10.1007/BF03263228

Cahyati, S., Isda M.N., and Lestari. W. (2016). Induksi tunas dari ekplan kotiledon dan epikotil in-vitro jeruk siam (Citrus nobilis Lour.) asal kampar pada media MS. Jurnal Riau Biologia. 1(5), 31-38

Cosic, T., Motyka, V., Raspor, M., Savic, J., Cingel, A., Vinterhalter, B., Vinterhalter, D., Travnockova, A., Dobrev, P., Bohanec B., & Nincovic. S. (2015). In vitro shoot organogenesis and comparative analysis of endogenous phytohormones in kohlrabi (Brassica oleracea var. Gongylodes): effects of genotype, explant type and applied cytokinins. PCTOC. 121,741-760

Das, A., Kesari, V., & Rangan, L. (2010). Plant regeneration in Curcuma species and assessment of genetic stability of regenerated plants. Biologia Plantarum, 54(3), 423-429. doi:10.1007/s10535-010-0077-0

Hendrikos, R., Marusin, N., and Tjong. D.H. (2014). Efek ekstrak etanol rimpang temu mangga (Curcuma mangga Val.) terhadap sel ? pankreas mencit putih yang diinduksi aloksan secara histologis. Jurnal Biologi Universitas Andalas. 3(4), 317-323

Herdiyanto, D. (2013). Rancangan Acak Lengkap. Jurusan Ilmu Tanah Universitas Padjajaran, Bandung

Hutami, S. dan Purnamaningsih, R. (2003). Perbanyakan klonal temu mangga (Curcuma mangga) melalui kultur in vitro. Balai Penelitian Bioteknologi dan Sumberdaya Genetik Pertanian. Bogor. 9(1), 39-44

Jala, A. (2013). The effect of the 2, 4-dichlorophenoxy acetic acid, benzyl adenine and paclobutrazol, on vegetative tissue-derived somatic embryogenesis in turmeric (Curcuma var. Chattip). Int. Trans. J. Eng. Manag. Sci. Tech. 4(2), 105-110.

Klerk, G.J. (2002). Plant hormones in tissue culture. Biochemical Plant Cell and Tissue Culture, Duchefa Biochemiev. 19-23.

Lakshmi, S., Padmaja, G., and Remani, P. (2011). Antitumour effects of isocurcumenol isolatedfrom Curcuma zedoaria rhizomes on human and murine cancer cells. International Journal of Medicinal Chemistry, 2011, 1-13. http://dx.doi.org/10.1155/2011/253962.

Mastuti, R. (2017). Dasar-dasar Kultur Jaringan Tumbuhan. Universitas Brawijaya Press, Malang

Miryam, A., Suliansyah, I. dan Djamaran, A. (2008). Multiplikasi jeruk kacang (Citrus nobilis L.) pada beberapa konsentrasi NAA dan BAP pada media WPM secara in vitro. Jerami, 1(2), 1-8.

Murwanti, R., Meiyanto, E., Nurrochmad, A., and Kristina, D.W. (2004). Efect ekstrak etanol rimpang temu putih (Curcuma zedoaria Roch.) terhadap pertumbuhan tumor paru fase post inisiasi pada mencit betina diinduksi Benzo[a]piren. Majalah Farmasi Indonesia, 15(1), 7-12.

Pamungkas, S.S.T. (2015). Pengaruh konsentrasi NAA dan BAP terhadap pertumbuhan tunas eksplan tanaman pisang cavendish (Musa paradisiaca l.) melalui kultur in vitro. Gontor Agrotech Science Journal, 2(1), 31-45.

Putri, M.S. (2014). White turmeric (Curcuma zedoaria) its chemical substance and the pharmacological benefit. Jurnal Majory, 3(7), 88-93

Raihana, R., Faridah, Q.Z., Julia, A.A., Abdelmageed, A.H.A., & Kadir, M.A. (2011). In vitro culture of Curcuma mangga from rhizome bud. J. Med. Plants Res, 5(28), 6418–6422.

Saha, K. , Sinha, R., Basak, S. and Sinha, S. (2016) ISSR fingerprinting to ascertain the genetic relationship of curcuma sp. of tripura. American Journal of Plant Sciences, 7, 259-266. doi: 10.4236/ajps.2016.72025

Sembiring, W.S., dan Suarnella. D.T. (2012). Efektivitas minyak atsiri rimpang kunyit putih Curcuma zedoaria sebagai larvasida terhadap nyamuk Aedes aegypti. Jurnal Buski, 4(2), 80-86.

Sharma, G.K., Jagetiya, S. Dashora, R. (2015). General Techniques of Plant Tissue Culture. Lulu Press Inc. Raleigh, North Carolina

Shukla S K, Shukla, S., Koche , V., and Mishra, S. K. (2007). In vitro propagation of tikhur (Curcuma angustifolia Roxb.): A starch yielding plant. Indian J. Biotech., 6, 274-276

Singh, W.R., Singh, H.B., Devi, S.S., Singh, W.N., Singh, N.M., & Devi, Y.P. (2015). Conservation of Curcuma caesia by in vitro techniques. Helix, 2, 708–713

Sintha, D. (2017). Pengaruh BAP dan kinetin terhadap pertumbuhan tunas pisang barangan (Musa paradisiaca L.) secara in vitro. Skripsi. Universitas Bengkulu, Bengkulu.

Siregar, L.H., Siregar, L.A.M., dan Putri. L.A.P. (2013). Pengaruh ?-benzil amino purin dan ?-asam asetat naftalena terhadap pertumbuhan akar Boesenbergia flava secara in-vitro. Agroekoteknologi, 1(3), 511-522.

Syukur, C. (2003). Temu Putih Tanaman Obat Anti Kanker. Penerbit Swadaya, Jakarta.

Waryastuti, D.E., Setyobudi, L. dan Wardiyati, T. (2017). Pengaruh tingkat konsentrasi 2,4-D dan BAP pada media MS terhadap induksi kalus embriogenik temulawak (Curcuma xanthorrhiza Roxb.). Jurnal Produksi Tanaman, 5(1),140-149.

Widono, M.S., dan Parfiani, N. (2002). Curcuma zedoaria Roch. Kajian pustaka kandungan kimia dan aktifitas farmakologik. Artocarpus, 2(1), 1-10.

Yulizar, D.R., Noli, Z.A., and Idris, M. (2014). Induksi tunas kunyit putih (Curcuma zedoaria Roscoe) pada media MS dengan penambahan berbagai konsentrasi BAP dan sukrosa secara in vitro. Jurnal Biologi Universitas Andalas, 3(4): 310-316.

Zulkarnain, H., (2009). Kultur Jaringan Tanaman. Jakarta, Bumi Aksara




DOI: https://doi.org/10.31186/j.agritropica.4.1.82-92

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