Main Article Content
Abstract
Begomovirus infection, which causes yellow leaf curl disease, is commonly found in the Bengkulu province, affecting various plants, including chilli, melon, cucumber, papaya, and weeds. The viruses reported to date in long bean plants include Mungbean yellow mosaic India virus (MYMIV) and Bean common mosaic virus (BCMV). This study aimed to detect viruses in long bean samples collected from Musi Rawas (South Sumatra) and North Bengkulu (Bengkulu), areas where yellow mosaic symptoms resembling those caused by Begomovirus infection were observed. Virus detection was performed using PCR-based DNA analysis with specific MYMIV (MY1/MY2) primers, targeting a DNA fragment of approximately 238 bp. The results revealed that yard-long bean plants from Musi Rawas were positively infected with MYMIV, while those from North Bengkulu tested negative for MYMIV. Begomovirus or other viral infections may cause the yellow mosaic symptoms observed in North Bengkulu yard long beans. Therefore, further investigations using additional primers are needed to identify the specific virus responsible for the symptoms in this region.
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Copyright (c) 2024 Mimi Sutrawati, Ewa Aulia

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References
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- Aulia, E., Sutrawati, M., & Pamekas, T. (2022). Deteksi molekuler dan analisis genetik Begomovirus pada tanaman cabai di Desa Pematang Donok. Jurnal Ilmu-Ilmu Pertanian Indonesia, 24(2), 69–74. https://doi.org/10.31186/jipi.24.2.69-74
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- Pertiwi, M. A. K. P., Hartono, S., Somowiyarjo, S., Sulandari, S., & Kandito, A. (2021). Identifikasi Molekuler Mungbean Yellow Mosaic India Virus dan betasatelit yang berasosiasi dengan gejala mosaik kuning pada kacang panjang di Sleman, Yogyakarta. Jurnal Fitopatologi Indonesia, 17(6), 251–260. https://doi.org/10.14692/jfi.17.6.251-260
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- Sutrawati, M., Parwito, P., Zarkani, A., Sariasih, Y., & Ganefianti, D. W. (2021). First report of Begomovirus infection on papaya in Bengkulu, Indonesia. Jurnal Hama dan Penyakit Tumbuhan Tropika, 21(1), 49–55. https://doi.org/10.23960/jhptt.12149-55
- Sutrawati, M., Sipriyadi, S., Sihotang, Y. K. S., Hutasoit, C. M., Parwito, P., & Aulia, E. (2022, May). Tomato Leaf Curl New Delhi Virus associated with yellow mosaic disease of cucumber (Cucumis sativus) in Bengkulu, Indonesia. In 7th International Conference on Biological Science (ICBS 2021) (pp. 1–4). Atlantis Press. https://www.atlantis-press.com/proceedings/icbs-21/125974073
- Tsai, W. S., Shih, S. L., Rauf, A., Safitri, R., Hidayati, N., Huyen, B. T. T., & Kenyon, L. (2013). Genetic diversity of legume yellow mosaic begomoviruses in Indonesia and Vietnam. Annals of Applied Biology, 163, 367–377. https://doi.org/10.1111/aab.12063
- Verma, H. N., Baranwal, V. K., & Srivastava, S. (1998). Antiviral substances of plant origin. In A. Hadidi, R. K. Khetarpal, & H. Koganezawa (Eds.), Plant viruses diseases control (pp. 154–162). APS Press.
- Zerbini, F. M., Briddon, R. W., Idris, A., Martin, D. P., Moriones, E., Navas-Castillo, J., & ICTV Report Consortium. (2017). ICTV virus taxonomy profile: Geminiviridae. Journal of General Virology, 98(2), 131–133. https://doi.org/10.1099/jgv.0.000738
References
Agrios, G. N. (2005). Plant pathology. Elsevier.
Anggraini, S., & Hidayat, S. H. (2014). Sensitivitas metode serologi dan polymerase chain reaction untuk mendeteksi Bean common mosaic potyvirus pada kacang panjang. Jurnal Fitopatologi Indonesia, 10(1), 17. https://doi.org/10.14692/jfi.10.1.17
Aulia, E., Sutrawati, M., & Pamekas, T. (2022). Deteksi molekuler dan analisis genetik Begomovirus pada tanaman cabai di Desa Pematang Donok. Jurnal Ilmu-Ilmu Pertanian Indonesia, 24(2), 69–74. https://doi.org/10.31186/jipi.24.2.69-74
Damayanti, T. A., Alabi, O. J., Naidu, R. A., & Rauf, A. (2009). Severe outbreak of a yellow mosaic disease on the yard long bean in Bogor, West Java. Hayati Journal of Biosciences, 16(2), 78–82.
Deepthi, N., Madhusudhan, K. N., Udayashankar, A. C., Kumar, H. B., Prakash, H. S., & Shetty, H. S. (2007). Effect of plant extracts and acetone precipitated proteins from six medicinal plants against tobamovirus infection. International Journal of Virology, 3(2), 80–87.
Doyle, J. J., & Doyle, J. L. (1987). A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin, 19(1), 11–15.
King, A. M. Q., Adams, M. J., Carstens, E. B., & Lefkowitz, E. J. (2012). Virus taxonomy: Classification and nomenclature of viruses. Academic Press.
Marpaung, N. K. B., Sutrawati, M., Ganefianti, D. W., Novanda, R. R., & Pamekas, T. (2023). Infeksi Ageratum yellow vein virus pada gulma Crassocephalum crepidioides di Bengkulu. Jurnal Fitopatologi Indonesia, 19(1), 39–44. https://doi.org/10.14692/jfi.19.1.39-44
Melinda, Damayanti, T. A., & Hidayat, S. H. (2015). Identifikasi molekuler Bean Common Mosaic Virus yang berasosiasi dengan penyakit mosaik kuning kacang panjang. Jurnal Hama dan Penyakit Tumbuhan Tropika, 15(2), 132–140. https://doi.org/10.23960/j.hptt.215132-140
Mulyadi, D., Sulandari, S., Hartono, S., & Somowiyarjo, S. (2021). Distribution, host range, and detection of seed-borne yellow mosaic disease on yardlong beans (Vigna unguiculata subsp. sesquipedalis) in the special region of Yogyakarta, Indonesia. Biodiversitas Journal of Biological Diversity, 22(9). https://doi.org/10.13057/biodiv/d220942
Mustika, A. K. P., Sedyo, H., Susanto, S., Sri, S., & Argawi, K. (2021). Identifikasi molekuler Mungbean yellow mosaic India virus dan betasatelit yang berasosiasi dengan gejala mosaik kuning pada kacang panjang di Sleman, Yogyakarta. Jurnal Fitopatologi Indonesia, 17(6), 251–260. https://doi.org/10.14692/jfi.17.6.251–260
Nurulita, S., Hidayat, S. H., Mutaqin, K. H., & Thomas, J. (2015). Molecular characterization of Begomovirus infecting yard long bean (Vigna unguiculata subsp. sesquipedalis) in Java, Indonesia. BIOTROPIA-The Southeast Asian Journal of Tropical Biology, 22(1). https://doi.org/10.11598/btb.2015.22.1.401
Nurulita, S., Mutaqin, K., & Hidayat, S. (2023). Development of specific detection for Mungbean Yellow Mosaic India Virus infecting yard long bean in Java, Indonesia. Jurnal Fitopatologi Indonesia, 19(2), 83–88. https://doi.org/10.14692/jfi.19.2.83-88
Pertiwi, M. A. K. P., Hartono, S., Somowiyarjo, S., Sulandari, S., & Kandito, A. (2021). Identifikasi Molekuler Mungbean Yellow Mosaic India Virus dan betasatelit yang berasosiasi dengan gejala mosaik kuning pada kacang panjang di Sleman, Yogyakarta. Jurnal Fitopatologi Indonesia, 17(6), 251–260. https://doi.org/10.14692/jfi.17.6.251-260
Purwaningsih, N. N. A., Puspawati, N. M., & Nyana, I. D. N. (2016). Pengaruh penyakit virus mosaik dan kuning terhadap hasil panen tanaman kacang panjang (Vigna sinensis) di Desa Perean, Baturiti, Tabanan. E-Jurnal Agroekoteknologi Tropika, 5(3), 212–221.
Supyani, Septariani, D. N., & Hadiwiyono, A. (2020). Symptoms variation of viral infections in yardlong bean. Jurnal Hama dan Penyakit Tumbuhan Tropika, 20(1), 28–36. https://doi.org/10.23960/j.hptt.12028-36
Sutrawati, M., Hidayat, S. H., Soekarno, B. P. W., & Nurmansyah, A. (2020). Yellow mosaic disease on soybean. Jurnal Fitopatologi Indonesia, 16(1), 30–36. https://doi.org/10.14692/jfi.16.1.30-36
Sutrawati, M., Parwito, P., Zarkani, A., Sariasih, Y., & Ganefianti, D. W. (2021). First report of Begomovirus infection on papaya in Bengkulu, Indonesia. Jurnal Hama dan Penyakit Tumbuhan Tropika, 21(1), 49–55. https://doi.org/10.23960/jhptt.12149-55
Sutrawati, M., Sipriyadi, S., Sihotang, Y. K. S., Hutasoit, C. M., Parwito, P., & Aulia, E. (2022, May). Tomato Leaf Curl New Delhi Virus associated with yellow mosaic disease of cucumber (Cucumis sativus) in Bengkulu, Indonesia. In 7th International Conference on Biological Science (ICBS 2021) (pp. 1–4). Atlantis Press. https://www.atlantis-press.com/proceedings/icbs-21/125974073
Tsai, W. S., Shih, S. L., Rauf, A., Safitri, R., Hidayati, N., Huyen, B. T. T., & Kenyon, L. (2013). Genetic diversity of legume yellow mosaic begomoviruses in Indonesia and Vietnam. Annals of Applied Biology, 163, 367–377. https://doi.org/10.1111/aab.12063
Verma, H. N., Baranwal, V. K., & Srivastava, S. (1998). Antiviral substances of plant origin. In A. Hadidi, R. K. Khetarpal, & H. Koganezawa (Eds.), Plant viruses diseases control (pp. 154–162). APS Press.
Zerbini, F. M., Briddon, R. W., Idris, A., Martin, D. P., Moriones, E., Navas-Castillo, J., & ICTV Report Consortium. (2017). ICTV virus taxonomy profile: Geminiviridae. Journal of General Virology, 98(2), 131–133. https://doi.org/10.1099/jgv.0.000738