Main Article Content
Abstract
Penelitian ini dirancang untuk menguji bioavailabilitas vitamin E yang dilarutkan dengan menggunakan pelarut yang berbeda, yaitu surfaktan tween 80 dan etanol pada bahan pengencer semen Andromed. Perlakuan yang diberikan pada penelitian ini adalah bahan antioksidan vitamin E dan bahan pelarut minyak yang terbagi dalam kelompok Andromed kontrol (P0), Andromed Vit E (0.2 gr) + etanol (P1), Andromed vit.E (0.2 gr) + tween 80 (P2), Andromed vit. E (0.4 gr) + etanol (P3) dan Andromed Vit. E (0.4 gr) + tween 80 (P4). Metode penelitian menggunakan pendekatan eksperimental, dan dianalisis secara deskriptif. Vitamin E dikombinasikan dengan pelarut sesuai perlakuan yang selanjutnya disebut sediaan vitamin E. Sediaan vitamin E kemudian dicampurkan kedalam bahan pengencer. Sampel bahan pengencer tersebut dianalisis menggunakan analisis DPPH untuk mengukur aktivitas dari vitamin E di dalam bahan pengencer semen. Hasil penelitian menunjukkan nilai aktivitas antioksidan bahan pengencer yang diberikan kombinasi Vit. E dan tween 80 paling tinggi sebesar 91.29% sedangkan bahan pengencer dengan kombinasi Vit. E dan etanol aktivitas antioksidan tertingginya sebesar 57.45% dan hasil paling rendah pada bahan pengencer control tanpa penambahan vitamin E. Tween 80 sebagai pelarut vitamin E memiliki kemampuan yang lebih baik dalam melarutkan vitamin E dalam bahan pengencer semen jika dibandingkan dengan etanol.
Keywords
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References
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References
Azawi, O. I., Hussein, E. K. 2013. Effect of vitamins C or E supplementation to tris diluent on the semen quality of awassi rams preserved at 5°C. Vet Res Forum. 4: 157–160.
Benhenia, K., Lamara, A., Fatmi, S., Iguer-Ouada, M. 2016. Effect of cyclodextrins, cholesterol and vitamin E and their complexation on cryopreserved epididymal ram semen. Small Rum Res. 141: 29–35. DOI: 10.1016/j.smallrumres.2016.06.009
Breininger, E., Beorlegui, N. B., O’Flaherty, C. M., & Beconi, M. T. 2005. Alpha-tocopherol improves biochemical and dynamic parameters in cryopreserved boar semen. Theriogenology. 63: 2126–2135. DOI: 10.1016/j.theriogenology.2004.08.016
Dubbs, M. D. Dan Gupta, R. B. 1998. Solubility of vitamin e (?-tocopherol) and vitamin K 3 (Menadione) in ethanol-water mixture. Journal of Chemical and Engineering Data. 43(4): 590 591. DOI: 10.1021/je980017l
Flanagan, J., and Singh, H. 2006. Microemulsions; a potential delivery system for bioactives in food. Food Science and Nutrition. 46: 221- 237. DOI: 10.1080/10408690590956710
Gangwar, C., Kharche, S. D., Ranjan, R., Kumar, S., Goel, A. K., Jindal, S. K., & Agarwal, S. K. 2015. Effect of vitamin C supplementation on freezability of Barbari buck semen. Small Rum Res. 129: 104–107. DOI: 10.1016/j.smallrumres.2015.06.002
Herdis, Kusuma, I., Surachman, M., Riza, M., Sutama, I.K., Inounu, I., Arifiantini, I. 2002. Peningkatan Kualitas semen beku domba garut melalui penambahan ? -Tokoferol ke dalam pengencer susu-skim kuning telur. Jurnal Ilmu Ternak dan Veteriner. 7: 12–17. DOI: 10.14334/jitv.v7i1.269
Herold, F. C., de Haas, K., Colenbrander, B., Gerber, D. 2006. Comparison of equilibration times when freezing epididymal sperm from African buffalo (Syncerus caffer) using Triladyl or AndroMed. Theriogenology. 66: 1123–1130. DOI: 10.1016/j.theriogenology.2006.03.007
Jusnita, N. 2014. Produksi Nanoemulsi Ekstrak Temulawak Dengan Metode Homogenisasi. Tesis, Institut Pertanian Bogor, Bogor.
Minitub. 2001. Certificate Andromed. Minitub Abfullund Labortechnik GmbH and Co KG, Germany.
Mocé, E., Purdy, P. H., Graham, J. K. 2010. Treating ram sperm with cholesterol-loaded cyclodextrins improves cryosurvival. Anim Reprod Sci. 118: 236–247. DOI: 10.1016/j.anireprosci.2009.06.013
Moretti, E., Castellini, C., Mourvaki, E., Capitani, S., Geminiani, M., Renieri, T., Collodel, G. 2011. Distribution of ?- and ?-tocopherols in seminal plasma and sperm fractions of men with normal and abnormal semen parameters. J Androl. 32: 232–9. DOI: 10.2164/jandrol.109.009936
Saberi, A. H., Fang, Y., Mcclements, D.J. 2013. Fabrication of vitamin E- enriched nanoemulsions by spontaneous emulsification: effect of propylene glycol and ethanol on formation, stability, and properties. Food Research International. 54; 812-820. DOI: 10.1016/j.foodres.2013.08.028
Setya, S., Talegaonkar, S., Razdan, B. K. 2014. Nanoemulsions:Formulation methods and stability aspects. World Journal of Pharmacy and Pharmaceutical Sciences. 3: 2214–2228.
Winangun, K. 2019. Kualitas Larutan Pengencer Dan Kualitas Semen Domba Pada Temperatur Penyimpanan Yang Berbeda. Kandaga–Media Publikasi Ilmiah Jabatan Fungsional Tenaga Kependidikan, 1(1). DOI: 10.24198/kandaga.v1i1.21217
Yang, Y., Julian, D., Clements, M. 2013. Encapsulation of vitamin E in edible emulsions fabricated using a natural surfactant. Food Hydrocolloids. 30: 712–720. DOI: 10.1016/j.foodhyd.2012.09.003