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Abstract

From the past until now, weed control using synthetic herbicides has remained the primary method. The use of synthetic herbicides in modern agricultural processes still has negative impacts on the environment, health, and weeds, thereby potentially causing weed resistance. Therefore, planting herbal bioherbicides is a more environmentally friendly method of weed control. Sorghum (Sorghum bicolor L. Moench), in particular, has allelopathic exudates that are very troublesome for weeds. This study focused on analyzing the chemical allelopathy of sorghum root extracts and identifying candidate bioherbicide compounds in the roots using Gas Chromatography-Mass Spectrometry (GC-MS) analysis. Sorghum was extracted using a cold maceration method obtained from a mixture of solvents with different configurations. Then, through GC-MS, the solvents were extracted, and the compounds were separated. As a result, through GC-MS analysis, 20 compounds were identified, most of which were detected in the steroid, sterol, and fatty acid derivative groups. The most prominent compounds detected were 4, 22-stigmastadien-3-one (20.74%); stigmasterol (total 16.47%); cholest-4-en-26-oic acid, 3-oxo- (11.68%); and 9-octadecenamide (Z) (7.69%). Several polar compounds, such as 9-octadecenamide (Z) and cyclohexanol derivatives, exhibited excellent capabilities. Therefore, sorghum root extract contains allelochemical compounds that have the potential to be sustainable and environmentally friendly bioherbicides.

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Copyright (c) 2026 Edi Susilo, Nanik Setyowati, Uswatun Nurjanah, Hesti Pujiwati, Riwandi

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How to Cite
Susilo, E., Setyowati, N., Nurjanah, U., Pujiwati, H., & Riwandi. (2026). Profile of Allelochemical Compounds in Ratun Sorghum Root Extract Based on GC-MS Analysis as a Bioherbicide Candidate: Profil Senyawa Alelokimia Ekstrak Akar Ratun Sorgum Berdasarkan Analisis GC-MS sebagai Kandidat Bioherbisida. PENDIPA Journal of Science Education, 10(1), 211–222. https://doi.org/10.33369/pendipa.10.1.211-222
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