Main Article Content
Abstract
Talipuk seed flour is processed from mature talipuk seeds. This flour is commonly used as a raw material for traditional cakes and as a substitute for wheat flour by the people of South Kalimantan. Talipuk seeds contain various compounds that function well for health. However, talipuk seeds are less desirable because they have a sandy texture and contain anti-nutritional compounds that cause a less desirable flavor. This can be overcome by fermentation by yeast. This study was conducted to analyze the functional properties (total phenolic content by the Folin-Ciocalteau method and antioxidant activity with the free radical inhibitor DPPH (2,2-diphenyl-1-picrylhydrazil) in talipuk seed flour fermented with various yeasts. Talipuk seeds were fermented by fermentation spontaneously, tape yeast, baker's yeast and tempeh yeast for 48 hours. The results of the fermentation are dried using an oven at 60oC for 8 hours or with a hot sun for ± 12 hours. Then mashed and sieved with a 60 mesh flour sieve to obtain fermented talipuk seed flour Based on the results of research, the fermentation of talipuk seed flour can increase the total phenolic content and antioxidant activity of talipuk seed flour. The highest total phenolic in the fermentation of talipuk seed flour using baker's yeast is 61.22 mg/g GAE and tape yeast 56.40 mg/g GAE, while the highest antioxidant activity in unfermented talipuk bij flour was 84.85%, followed by fermentation of talipuk seed flour using 47.34% baker's yeast and 36.34 % tape yeast. The use of baker's yeast and tape yeast in the fermentation of talipuk seed flour gave better total phenolic and antioxidant activity test results than spontaneous fermentation and fermentation using tempeh yeast.
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References
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- Singleton, V. L., & Rossi, J. A. (1965). Colorimetry of Total Phenolics with Phosphomolybdic-Phosphotungstic Acid Reagents. American Journal of Enology and Viticulture, 16(3).
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- Wu, Q., Qu, H., Jia, J., Kuang, C., Wen, Y., Yan, H., & Gui, Z. (2015). Characterization, antioxidant and antitumor activities of polysaccharides from purple sweet potato. Carbohydrate Polymers, 132, 31–40. https://doi.org/10.1016/J.CARBPOL.2015.06.045
- Yen, G. C., & Hung, C. Y. (2000). Effects of alkaline and heat treatment on antioxidative activity and total phenolics of extracts from Hsian-tsao (Mesona procumbens Hemsl.). Food Research International, 33(6), 487–492. https://doi.org/10.1016/S0963-9969(00)00073-9
References
Adetuyi, F. O., & Ibrahim, T. A. (2014). Effect of Fermentation Time on the Phenolic, Flavonoid and Vitamin C Contents and Antioxidant Activities of Okra (Abelmoschus esculentus) Seeds. Nigerian Food Journal, 32(2), 128–137. https://doi.org/10.1016/S0189-7241(15)30128-4
Dungir, S. G., Katja, D. G., & Kamu, V. S. (2012). Aktivitas Antioksidan Ekstrak Fenolik dari Kulit Buah Manggis (Garcinia mangostana L.). Jurnal MIPA, 1(1), 11. https://doi.org/10.35799/jm.1.1.2012.424
F. Kawamura, S. F.M. Ramle, O. Sulaiman, & Hashim, R. (2008). Study on antioxidant activities, total phenolic compound and antifungal properties of some Malaysian timbers from selected hardwoods species. Proceedings International Conference on Environmental Research and Technology. https://doi.org/10.3/JQUERY-UI.JS
Fitrial, Y. (2011). Aktivitas Antibakteri Ekstrak Etil Asetat Biji Teratai (Nymphaea Pubescens Willd) Akibat Pemanasan. Jurnal Pengolahan Hasil Perikanan Indonesia, 14(1), 43–48. https://doi.org/10.17844/JPHPI.V14I1.3427
Indra, I., Nurmalasari, N., & Kusmiati, M. (2019). Fenolik Total, Kandungan Flavonoid, dan Aktivitas Antioksidan Ekstrak Etanol Daun Mareme (Glochidion arborescense Blume.). Jurnal Sains Farmasi & Klinis, 6(3), 206. https://doi.org/10.25077/jsfk.6.3.206-212.2019
Khairina, R., Fitrial, Y., Khotimah, I. K., & Soetikno, N. (2020). Pelatihan Pengolahan Klemben Berbahan Tepung Biji Teratai. Jurnal Masyarakat Mandiri) |, 4(5), 764–774. https://doi.org/10.31764/jmm.v4i5.2954
Khan, A., Khan, S., Khan, M. A., Qamar, Z., & Waqas, M. (2015). The uptake and bioaccumulation of heavy metals by food plants, their effects on plants nutrients, and associated health risk: a review. Environmental Science and Pollution Research 2015 22:18, 22(18), 13772–13799. https://doi.org/10.1007/S11356-015-4881-0
Kiay, N., & Suryanto, E. (2019). Efek Lama Perendaman Ekstrak Kalamansi (Citrus Microcarpa) Terhadap Aktivitas Antioksidan Tepung Pisang Goroho (Musa spp.). Chemistry Progress, 4(1), 27–33. https://doi.org/10.35799/cp.4.1.2011.26502
Kil, H. Y., Seong, E. S., Ghimire, B. K., Chung, I. M., Kwon, S. S., Goh, E. J., Heo, K., Kim, M. J., Lim, J. D., Lee, D., & Yu, C. Y. (2009). Antioxidant and antimicrobial activities of crude sorghum extract. Food Chemistry, 115(4), 1234–1239. https://doi.org/10.1016/J.FOODCHEM.2009.01.032
Lestario, L. N., Sugiarto, S., & Timotius, K. H. (2008). Aktifitas Antioksidan dan Kadar Fenolik Total dari Ganggag Merah (Gracilaria verrucosa L.). J Teknol Dan Industri Pangan, 19(2), 131–138.
Luliana, S., Purwanti, N. U., & Manihuruk, K. N. (2016). Pengaruh Cara Pengeringan Simplisia Daun Senggani (Melastoma malabathricum L.) Terhadap Aktivitas Antioksidan Menggunakan Metode DPPH (2,2-difenil-1-pikrilhidrazil). Pharmaceutical Sciences and Research, 3(3), 2. https://doi.org/10.7454/psr.v3i3.3291
Othman, N. Ben, Roblain, D., Chammen, N., Thonart, P., & Hamdi, M. (2009). Antioxidant phenolic compounds loss during the fermentation of Chétoui olives. Food Chemistry, 116(3), 662–669. https://doi.org/10.1016/J.FOODCHEM.2009.02.084
Rahmi, N., Khairiah, N., Rufida, Hidayati, S., & Muis, A. (2020). Pengaruh Fermentasi Terhadap Total Fenolik, Aktivitas Penghambatan Radikal dan Aktivitas Antibakteri Ekstrak Tepung Biji Teratai (Nymphaea pubescens Willd.). Jurnal BIOPORAL Industri, 11(1), 9–18.
Singleton, V. L., & Rossi, J. A. (1965). Colorimetry of Total Phenolics with Phosphomolybdic-Phosphotungstic Acid Reagents. American Journal of Enology and Viticulture, 16(3).
Tandrianto, J., Mintoko, D. K., & Gunawan, S. (2014). Effect of fermentation using Lactobacillus plantarum on protein content of mocaf (modified cassava flour). Jurnal Teknik Pomits, 3(2), F-143-F145.
Wu, Q., Qu, H., Jia, J., Kuang, C., Wen, Y., Yan, H., & Gui, Z. (2015). Characterization, antioxidant and antitumor activities of polysaccharides from purple sweet potato. Carbohydrate Polymers, 132, 31–40. https://doi.org/10.1016/J.CARBPOL.2015.06.045
Yen, G. C., & Hung, C. Y. (2000). Effects of alkaline and heat treatment on antioxidative activity and total phenolics of extracts from Hsian-tsao (Mesona procumbens Hemsl.). Food Research International, 33(6), 487–492. https://doi.org/10.1016/S0963-9969(00)00073-9