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Abstract

Tempe is one type of food that is easily damaged. The use of probiotic microorganisms in the preparation of tempeh is expected to increase the storability and quality of tempe sensory. This study aims to determine the effect of concentrations of wheat flour and low temperature storage that can extend shelf life and maintain the sensory properties of probiotic soybean tempeh. This study used a Complete Randomized Block Design (RCBD) with two factors and three replications. The first factor is the concentration of flour consisting of 5 levels (0%, 0.2%, 0.4%, 0.6%, and 0.8% (b / v). The second factor is the low temperature of storage, which consists from 3 levels (5 ° C, 10 ° C, and 15 ° C). Data obtained were analyzed descriptively for parameters of determination of shelf life, while parameter data of sensory properties (color, aroma, texture, and overall acceptance) were tested for their similarities with Barlett's test, the addition of data with the Tuckey test, and further testing with orthogonal polynomial test at the level of 5% and 1 %.The results showed that the shelf life of soybean tempe treated with probiotic microorganisms ranged from 12.33 to 17.33 days. orthogonal polynomials of wheat flour and low temperature storage have an effect on increasing color scores and texture, but have a significant effect on decreasing aroma score and overall acceptance.The best probiotic soybean tempe is obtained by adding 0.6% flour and 5 ° C storage temperature keep the shelf life of 17.33 days, color score of 3.72 (bright white), aroma score of 2.78 (somewhat typical of tempe and mildly sour), texture score of 4.12 (compact and solid), score equal to overall acceptance 3.41 (rather like), and the total lactic acid bacteria ranged from 9.04 log CFU / g to 9.13 log CFU /g

Article Details

Author Biographies

Suharyono Suharyono, University of Lampung

Department of Agricultural Product Technology

Samsul Rizal, Universitas Lampung

Jurusan Teknologi Hasil Pertanian

Sussi Astuti, Universitas Lampung

Jurusan Teknologi Hasil Pertanian

Raisa Amalia, Alumni Universitas Lampung

Jurusan Teknologi Hasil Pertanian
How to Cite
Suharyono, S., Rizal, S., Astuti, S., & Amalia, R. (2019). EFFECT OF WHEAT FLOUR AND LOW TEMPERATURE STORAGE TO SHELF LIFE AND SENSORY PROPERTIES OF PROBIOTIC SOY TEMPEH. Jurnal Agroindustri, 9(1), 37–48. https://doi.org/10.31186/j.agroindustri.9.1.37-48

References

  1. Aptesia, L.T., Suharyono, dan H.A. Rasyid. 2013. Pemanfaatan Lactobacillus casei dan Tapioka dalam Upaya Menghambat Kerusakan Tempe Kedelai. Jurnal Teknologi Industri dan Hasil Pertanian. 18(2):175-184.
  2. Djaafar, T.F. dan E.S. Rahayu. 2006. Karakteristik Yogurt dengan Inokulum Lactobacillus yang Diisolasi dari Makanan Fermentasi Tradisional. Jurnal Agros. 8(1):73-80.
  3. Emilia, Q. 2015. Perilaku Bacillus cereus selama Fermentasi Tempe yang Diperkaya dengan Bakteri Asam Laktat. (Skripsi). Institut Pertanian Bogor. Bogor. 38 hlm.
  4. Efriwati, A. Suwanto, G. Rahayu, dan L. Nuraida. 2013. Population Dynamics of Yeast and
  5. Lactic Acid Bacteria (LAB) during Tempeh Production. Hayati Journal of Biosciences. 20(2):57-64.
  6. Feng, X.M., Eriksson, R.B. Anders, and S. Johan. 2005. Growth of Lactic Acid Bacteria and
  7. Rhizopus oligosporus during Barley Tempeh Fermentation. International Journal of Food Microbiology. 104(3):249–256.
  8. Hamzah, F., Marniza, dan S. Rizal. 2014. Pengaruh Konsentrasi Lactobacillus acidophilus dan Tepung Sagu terhadap Umur Simpan dan Sifat Sensori Tempe Kedelai. Jurnal Kelitbangan. 02(03):46-68.
  9. Hugenholtz, J. 2013. Traditional Biotechnology for New Foods and Beverages. Current Opinion in Biotechnology. 24 (2):155-159.
  10. Klaenhammer, T.R. 2007. Probiotics and Prebiotics. ASM Press. Washington DC. 891-907.
  11. Marini, S.M., Desniar, dan J. Santoso. 2016. Karakterisasi Minuman Jelly Probiotik dengan Penambahan Lactobacillus plantarum (SK5) Asal Bekasam selama Penyimpanan. Jurnal Pengolahan Hasil Perikanan Indonesia. 19(3):288-298.
  12. Moreno, M.F., J.J. Leisner, L.K. Tee, C. Ley, S. Radu, G. Rusul, M. Vancanneyt, and L. De Vuyst. 2002. Microbial Analysis of Malaysian Tempe and Characterization of Two Bacteriocins Produced by Isolates of Enterococcus faecium. Journal of Applied Microbiology. 92:147-157.
  13. Muslikhah, S., C. Anam, dan M.A.M. Andriani. Penyimpanan Tempe dengan Metode Modifikasi Atmosfer (Modified Atmosphere) untuk Mempertahankan Kualitas dan Daya Simpan. 2013. Jurnal Teknosains Pangan. 2(3):51-60.
  14. Nouts, M.J.R., and J.L. Kiers. 2005. A Review Tempe Fermentation, Innovation and Functionality: Update into The Third Millenium. Journal of Applied Microbiology. 98:789-805.
  15. Riniarsi, D. 2015. Outlook Komoditas Pertanian Tanaman Pangan: Kedelai. Pusat Data dan Sistem Informasi Pertanian, Kementerian Pertanian. Jakarta. 73 hlm.
  16. Sukardi, Wignyanto, dan P. Isti. 2008. Uji Coba Penggunaan Inokulum Tempe dari Kapang Rhyzopus oryzae dengan Substrat Tepung Beras dan Ubi Kayu pada Unit Produksi Tempe Sanan Kodya Malang. Jurnal Teknologi Pertanian. (9):207-215.
  17. Sunaryanto, R., E. Martius, dan B. Marwoto. 2014. Uji Kemampuan Lactobacillus casei sebagai Agensia Probiotik. Jurnal Bioteknologi &. Biosains. Indonesia. 1(1):9-15.
  18. Triyono, M., Nazaruddin, dan W. Werdiningsih. 2017. Uji Aktivitas Inokulum Tempe dari Bahan Limbah Kulit Pisang terhadap Mutu Tempe Kedelai. Jurnal Ilmu dan Teknologi Pangan. 3(1):200-206.
  19. Widowati, S., M.E. Yuniar, Christina, dan R. Holinesti. 2004. Analisis Kerusakan Produk Tempe Kedelai. (Laporan Mata Kuliah Pengawetan Pangan). Program Studi Ilmu Pangan, Sekolah Pascasarjana, Institut Pertanian Bogor. Bogor. 22 hlm.
  20. Winanti, R., S.H. Bintari, dan D. Mustikaningtyas. 2014. Studi Observasi Higienitas Produk
  21. Tempe Berdasarkan Perbedaan Metode Inokulasi. Unnes Journal of Life Science. 3(1):39-46.
  22. Winarno, F.G. 2004. Kimia Pangan dan Gizi. Gramedia Pustaka Utama. Jakarta. 253 hlm.