Main Article Content
Abstract
Meatballs are a processed-meat product which has high nutritional value and is classified as perishable food products. Therefore, preservative agents were needed to maintain the shelf life of meatball products. Natural preservative agents that can be used are antibacterial compounds derived from lactic acid bacteria. The objective of this study was to determine the use of cell-free supernatant antibacterial produced by Pediococcus pentosaceus BAF715 as a biopreservative of buffalo meatballs storage in cold temperatures. Buffalo meatballs were soaked in cell-free supernatant antibacterial for 30 minutes, then stored at cold temperatures for 0, 3, 6, 9, and 12 days were used as treatments. The results showed that the storage time of meatballs soaked in cell-free antibacterial supernatant can reduce the total bacteria, Staphylococcus aureus and kill Escherichia coli. The storage time can maintain pH and percentage of free water. Total bacteria and Staphylococcus aureus in 9 days of storage and it still within the requirements of the Indonesian National Standard (SNI 3818: 2014) regarding the quality of meatballs, while control meatballs (without soaked) were only up to 3 days of storage. It was concluded that the cell-free supernatant antibacterial produced by Pediococcus pentosaceus BAF715 was able to maintain the microbiological and physical quality of buffalo meatballs until 9 days of storage at cold temperatures.
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References
- Adeniyi,B.A., F.A. Ayeni, S.T. Ogunbanwo. 2006. Antogonistic activities of lactic acid bacteria isolated from Nigerian fermented dairy foof against organisms implicated in urinary tract infection. Biotechnolog 5(2): 183-188. doi:10.3923/biotech.2006.183.188.
- Afriani, Arnim, Y. Marlinda and Yuherman. 2018. Isolation and characterization of lactic acid bacteria proteases from bekasam for use as a beef tenderizer. Pakistan Journal of Nutrition (17):361-367. doi: 10.3923/pjn.2018.361.367.
- AOAC (Association Official Analitycal Chemistry). 2005. Official Method of Analysis. 18th Edition. Maryland (USA): AOAC International.
- Arief I.I , B. S. L. Jenie , T. Suryati , G. Ayuningtyas and A. Fuziawan. 2012. Antimicrobial activity of bacteriocin from indigenous lactobacillus plantarum 2c12 and its application on beef meatball as biopreservative. J.Indonesian Trop.Anim.Agric. 37(2) : 90-96. doi: 10.14710/jitaa 37.90-96.
- Arief, I.I., C. Budiman, B.S.L. Jenie, E. Andreas, and A. Yuneni. 2015. Plantaricin IIA-1A5 from Lactobacillus plantarum IIA-1A5 displays bactericidal activity against Staphylococcus aureus. Beneficial Microbes. 6(4): 603-613. doi. 10.3920/BM2014.0064
- Badan Standardisasi Nasional. 2014. Persyaratan Baso Daging. SNI 3818:2014. Jakarta.
- Cleveland, J., T.J. Montville, I.F. Nes, and M.L. Chikindas. 2001. Bacteriocins: Safe, natural antimicrobials for food preservation. Int. J. Food Microbiol. 71: 1-20
- Dhiman, A., A. Nanda, S. Ahmad, and B. Narasimhan. 2011. In vitro antimicrobial activity of methanolic leaf extract of psidium guajava L. J Pharm Bioallied Sci. 3(2): 226-9. doi: 10.4103/0975-7406.80776
- Diep D.B., D. Straume, M. Kjos, C. Torres, and I. F. Nes. 2009. An overview of the mosaic bacteriocin pln loci from Lactobacillus plantarum. Peptides. 30(8):1562-74. doi: 10.1016/j.peptides.2009.05.014
- Franciska J., I W. Suardana, dan I. N. Suarsana. 2018. Bakteriosin asal streptococcus bovis 9a sebagai biopreservatif pada daging sapi ditinjau dari uji eber. Indonesia Medicus Veterinus. 7(2): 158-167. doi: 10.19087/imv.2018.7.2.158
- Hafsan. 2014. Bakteriosin asal bakteri asam laktat sebagai biopreservatif pangan. J. Teknosains, 8(2): 175 – 184. doi:10.24252/teknosains.v8i2.1905.
- Hastaoglu, E., M.G. Sarac, Z.S. Keskin, and F. Yozgat. 2016. Effects of nisin and natamycin on microbiological and physiochemical qualities of meatball supplied from Yildizeli, Sivas. J Food Process Technol 2016, 7:12 (Suppl). doi: 10.4172/2157-7110.C1.056.
- Hastaoglu, E., M.G. Sarac, Z.S. Keskin, and F. Yozgat. 2017. The Effects of nisin and natamycin on the microbiological, chemical and sensorial qualities of meatballs. Cumhuriyet Sci. J. 38(4): 834-844. doi.10.17776/csj.349817.
- Hatta, T., R. Tanaka, and S. Ohmomo. 2010. Isolation and characteriation of plantaricin ASM1: A new bacteriocin produced by Lactobacillus plantarum A-1. J. Food Microbiol. 137: 94-99.
- Ismail M , R. Kautsar , P. Sembada , S. Aslimah dan I.I. Arief. 2016. Kualitas fisik dan mikrobiologis bakso daging sapi pada penyimpanan suhu yang berbeda. Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan. 04 (3) : 372-374.
- Kadariya J, T.C Smith, D. Thapaliya. 2014. Staphylococcus aureus and Staphylococcal foodborne disease: an ongoing challenge in public health. BioMed Research International, 827965. doi: 10.1155/2014/827965
- Kia, K.W., I.I. Arief, C. Sumantri and C. Budiman. 2016. Plantaricin IIA-1A5 from Lactobacillus plantarum IIA-1A5 retards pathogenic bacteria in beef meatball stored at room temperatur. Am. J Food Technol. 11 (1-2): 37-43. DOI: 10.3923/ajft.2016.37.43
- Kusumawati N, 2000. Peranan bakteri asam laktat dalam menghambat listeria monocitogenes dalam bahan pangan. Jurnal Teknologi Pangan dan Gizi. 1(1) : 14-28. doi: 10.33508/jtpg.v1i1.77
- Lapitan, R.M., A. N. Del Bario, O. Katsube, T. Ban-Tokuda, E. A. Orden, A. Y. Robles, L.C. Cruz, Y. Kanai and T. Fujihara. 2008. Comparison of carcass and meat characteristic of brahman grade cattle (bos indicus) and crossbred water buffalo (bubalus bubalis) fed on high roughage diet. Animal Science Journal 79 : 210-217. doi:10.1111/j.1740.0929.2008.00519.
- Savadogo, A., C.A.T. Ouattara, I.H.N. Bassole, and S.A. Traore. 2006. Bacteriocin and lactid acid bacteria-a minireview. Afr. J. Biotechnol. 5: 678-683.
- See, A.S., A.B. Salleh, F.A. Bakar, N.A. Yusof, A.S Abdulamir, and L.Y. Heng. 2010. Risk and health effect of boric acid. Am. J Appl. Sci. 7(5): 620-627.
- Sihombing, D.E., I.I. Arief and S. Budiarti, 2015. Application of antimicrobial agents produced by Lactobacillus plantarum IIA-1A5 as natural preservative on beef during room temperature storage. Adv. J. Food Sci. Technol., 8 (4) : 251-255. doi:10.19026/ajfst.8.1503.
- Sindelar, J.J., and A.L. Milkowski. 2011. Sodium nitrite in processed meat and poultry meat: A review of curing and examining the risk or benefit of its use. AMSA White Paper Series No. 3. American Meat Scince Association, USA. pp: 1-14.
- Soeparno. 2015. Ilmu dan Teknologi Daging. Gadjah Mada University Press, Yogyakarta
- Steel, R.G.D., dan J.H. Torrie. 1991. Prinsip dan Prosedur Statistik. Suatu Pendekatan Biometrik.Terjemahan. Alih bahasa. B. Sumantri. Gramedia. Jakarta.
- Soenarno M. S. , I. I. Arief, C. Sumantri, E. Taufik dan L. Nuraida. 2020. Karakterisasi plantarisin iia-1a5 sebagai antimikroba dan evaluasi aktivitas sediaan kering beku terenkapsulasi. Jurnal Aplikasi Teknologi Pangan 9 (1) : 30 – 37. doi: 10.17728/jatp.5480
- Suradi, K. 2012. Pengaruh lama penyimpanan pada suhu ruang terhadap perubahan nilai pH, TVB dan total bakteri daging kerbau. Jurnal Ilmu Ternak 2 (2): 9-12. doi: 10.24198/jit.v12i2.5121
- Pato U, Y. Yusuf, S. Fitriani, D. A. Fauzi, G. Ismadiah, M. Hidayah, and W. Sabiliani. 2022. Antibacterial Activity of Bacteriocin from pediococcus pentosaceus strain 2397 and application as biopreservative for fishballs. Philippine Journal of Science 151 (2): 713-725. doi: 10.24198/jit.v12i2.5121
- World Health Organization. 2011. Nitrate and Nitrite in Drinking-water. Geneva: WHO Press .
References
Adeniyi,B.A., F.A. Ayeni, S.T. Ogunbanwo. 2006. Antogonistic activities of lactic acid bacteria isolated from Nigerian fermented dairy foof against organisms implicated in urinary tract infection. Biotechnolog 5(2): 183-188. doi:10.3923/biotech.2006.183.188.
Afriani, Arnim, Y. Marlinda and Yuherman. 2018. Isolation and characterization of lactic acid bacteria proteases from bekasam for use as a beef tenderizer. Pakistan Journal of Nutrition (17):361-367. doi: 10.3923/pjn.2018.361.367.
AOAC (Association Official Analitycal Chemistry). 2005. Official Method of Analysis. 18th Edition. Maryland (USA): AOAC International.
Arief I.I , B. S. L. Jenie , T. Suryati , G. Ayuningtyas and A. Fuziawan. 2012. Antimicrobial activity of bacteriocin from indigenous lactobacillus plantarum 2c12 and its application on beef meatball as biopreservative. J.Indonesian Trop.Anim.Agric. 37(2) : 90-96. doi: 10.14710/jitaa 37.90-96.
Arief, I.I., C. Budiman, B.S.L. Jenie, E. Andreas, and A. Yuneni. 2015. Plantaricin IIA-1A5 from Lactobacillus plantarum IIA-1A5 displays bactericidal activity against Staphylococcus aureus. Beneficial Microbes. 6(4): 603-613. doi. 10.3920/BM2014.0064
Badan Standardisasi Nasional. 2014. Persyaratan Baso Daging. SNI 3818:2014. Jakarta.
Cleveland, J., T.J. Montville, I.F. Nes, and M.L. Chikindas. 2001. Bacteriocins: Safe, natural antimicrobials for food preservation. Int. J. Food Microbiol. 71: 1-20
Dhiman, A., A. Nanda, S. Ahmad, and B. Narasimhan. 2011. In vitro antimicrobial activity of methanolic leaf extract of psidium guajava L. J Pharm Bioallied Sci. 3(2): 226-9. doi: 10.4103/0975-7406.80776
Diep D.B., D. Straume, M. Kjos, C. Torres, and I. F. Nes. 2009. An overview of the mosaic bacteriocin pln loci from Lactobacillus plantarum. Peptides. 30(8):1562-74. doi: 10.1016/j.peptides.2009.05.014
Franciska J., I W. Suardana, dan I. N. Suarsana. 2018. Bakteriosin asal streptococcus bovis 9a sebagai biopreservatif pada daging sapi ditinjau dari uji eber. Indonesia Medicus Veterinus. 7(2): 158-167. doi: 10.19087/imv.2018.7.2.158
Hafsan. 2014. Bakteriosin asal bakteri asam laktat sebagai biopreservatif pangan. J. Teknosains, 8(2): 175 – 184. doi:10.24252/teknosains.v8i2.1905.
Hastaoglu, E., M.G. Sarac, Z.S. Keskin, and F. Yozgat. 2016. Effects of nisin and natamycin on microbiological and physiochemical qualities of meatball supplied from Yildizeli, Sivas. J Food Process Technol 2016, 7:12 (Suppl). doi: 10.4172/2157-7110.C1.056.
Hastaoglu, E., M.G. Sarac, Z.S. Keskin, and F. Yozgat. 2017. The Effects of nisin and natamycin on the microbiological, chemical and sensorial qualities of meatballs. Cumhuriyet Sci. J. 38(4): 834-844. doi.10.17776/csj.349817.
Hatta, T., R. Tanaka, and S. Ohmomo. 2010. Isolation and characteriation of plantaricin ASM1: A new bacteriocin produced by Lactobacillus plantarum A-1. J. Food Microbiol. 137: 94-99.
Ismail M , R. Kautsar , P. Sembada , S. Aslimah dan I.I. Arief. 2016. Kualitas fisik dan mikrobiologis bakso daging sapi pada penyimpanan suhu yang berbeda. Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan. 04 (3) : 372-374.
Kadariya J, T.C Smith, D. Thapaliya. 2014. Staphylococcus aureus and Staphylococcal foodborne disease: an ongoing challenge in public health. BioMed Research International, 827965. doi: 10.1155/2014/827965
Kia, K.W., I.I. Arief, C. Sumantri and C. Budiman. 2016. Plantaricin IIA-1A5 from Lactobacillus plantarum IIA-1A5 retards pathogenic bacteria in beef meatball stored at room temperatur. Am. J Food Technol. 11 (1-2): 37-43. DOI: 10.3923/ajft.2016.37.43
Kusumawati N, 2000. Peranan bakteri asam laktat dalam menghambat listeria monocitogenes dalam bahan pangan. Jurnal Teknologi Pangan dan Gizi. 1(1) : 14-28. doi: 10.33508/jtpg.v1i1.77
Lapitan, R.M., A. N. Del Bario, O. Katsube, T. Ban-Tokuda, E. A. Orden, A. Y. Robles, L.C. Cruz, Y. Kanai and T. Fujihara. 2008. Comparison of carcass and meat characteristic of brahman grade cattle (bos indicus) and crossbred water buffalo (bubalus bubalis) fed on high roughage diet. Animal Science Journal 79 : 210-217. doi:10.1111/j.1740.0929.2008.00519.
Savadogo, A., C.A.T. Ouattara, I.H.N. Bassole, and S.A. Traore. 2006. Bacteriocin and lactid acid bacteria-a minireview. Afr. J. Biotechnol. 5: 678-683.
See, A.S., A.B. Salleh, F.A. Bakar, N.A. Yusof, A.S Abdulamir, and L.Y. Heng. 2010. Risk and health effect of boric acid. Am. J Appl. Sci. 7(5): 620-627.
Sihombing, D.E., I.I. Arief and S. Budiarti, 2015. Application of antimicrobial agents produced by Lactobacillus plantarum IIA-1A5 as natural preservative on beef during room temperature storage. Adv. J. Food Sci. Technol., 8 (4) : 251-255. doi:10.19026/ajfst.8.1503.
Sindelar, J.J., and A.L. Milkowski. 2011. Sodium nitrite in processed meat and poultry meat: A review of curing and examining the risk or benefit of its use. AMSA White Paper Series No. 3. American Meat Scince Association, USA. pp: 1-14.
Soeparno. 2015. Ilmu dan Teknologi Daging. Gadjah Mada University Press, Yogyakarta
Steel, R.G.D., dan J.H. Torrie. 1991. Prinsip dan Prosedur Statistik. Suatu Pendekatan Biometrik.Terjemahan. Alih bahasa. B. Sumantri. Gramedia. Jakarta.
Soenarno M. S. , I. I. Arief, C. Sumantri, E. Taufik dan L. Nuraida. 2020. Karakterisasi plantarisin iia-1a5 sebagai antimikroba dan evaluasi aktivitas sediaan kering beku terenkapsulasi. Jurnal Aplikasi Teknologi Pangan 9 (1) : 30 – 37. doi: 10.17728/jatp.5480
Suradi, K. 2012. Pengaruh lama penyimpanan pada suhu ruang terhadap perubahan nilai pH, TVB dan total bakteri daging kerbau. Jurnal Ilmu Ternak 2 (2): 9-12. doi: 10.24198/jit.v12i2.5121
Pato U, Y. Yusuf, S. Fitriani, D. A. Fauzi, G. Ismadiah, M. Hidayah, and W. Sabiliani. 2022. Antibacterial Activity of Bacteriocin from pediococcus pentosaceus strain 2397 and application as biopreservative for fishballs. Philippine Journal of Science 151 (2): 713-725. doi: 10.24198/jit.v12i2.5121
World Health Organization. 2011. Nitrate and Nitrite in Drinking-water. Geneva: WHO Press .