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Abstract
This study aims to determine the effect of the thawing technique on the quality of Boer goat semen with tris aminomethane diluent supplemented with quercetin antioxidants. The materials used in this research are the semen of a Boer goat, which was collected with an artificial vagina. This study used experimental laboratory methods. The design pattern used is a Factorial Complete Randomized Design, which consists of two factors. Each treatment was repeated 4 times. The first factor is the thawing temperature with 3 levels of treatment those were 32°C, 37°C, and 40°C. The second factor is the thawing time with 3 treatment levels: 30 seconds, 60 seconds and 90 seconds. The variables were the motility percentage, viability, abnormality and membrane integrity. The results showed that thawing temperature had a significantly different effect (P<0.01) on the percentage of motility, viability, abnormality and membrane integrity. Thawing time had a significantly different effect (P<0.01) on the percentage of motility, abnormality and membrane integrity and gave affects significant (P<0.05) in the percentage of viability. Meanwhile, the interaction between thawing temperature and time is insignificant (P>0.05). The best result shown after thawing is in treatment thawing temperature at 37°C and treatment thawing time at 30 seconds.
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References
- Agustian, M.F., M.N. Ihsan dan N. Isnaini. 2014. Pengaruh Lama Simpan Semen Dengan Pengencer Tris Aminomethan Kuning Telur Pada Suhu Ruang Terhadap Kualitas Spermatozoa Kambing Boer. J. Ternak Tropika. 15 (2): 1-6.
- Ansary, M.S., A. Bushra, Rakha and S. Akher. 2010. Effect of Straw Size and Thawing Time on Quality of Cryopreserved Buffalo (Bubalus bubalis) Semen. J Reproductive Biology. 11(1): 49-54.
- Aprilina, N., S. Suharyati dan P.E. Santosa. 2014. Pengaruh Suhu Dan Lama Thawing di Dataran Rendah Terhadap Kualitas Semen Beku Sapi Simmental. Jurnal Ilmiah Peternakan Terpadu. 2(3): 96-102.
- Datta, U., Sekar, M. C., Hembram, M. L., Dasgupta, R., 2009. Development of a New Methode to Preserve Caprine Cauda Epididymal Spermatozoa in situ at 10°C. Procedings. Departement of Veterinary Gynaecology & Obstetrics Faculty of Veterinary and Animal Sciences West Bengal University of Animal and Fishery Sciences. Kolkata West Bengal. India.
- Fitrik dan N. Supartini. 2012. Pengaruh Suhu dan Lama Thawing terhadap Kualitas Spermatozoa Kambing Peranakan Etawa. Buana Sains. 12(1): 81-86.
- Hoesni, F. 2013. Pengaruh Penggunaan Metode Thawing yang Berbeda terhadap Kualitas Spermatozoa Semen Sapi Perah Berpengencer Tris Sitrat Kuning Telur. Jurnal Ilmiah Universitas Batanghari Jambi. 13(4): 118-126.
- Jamadi, A.S., E. Ahmad, M. Ansari and H. Kohram. 2017. Antioxidant effect of quercetin in an extender containing DMA or glycerol on freezing capacity of goat semen. Cryobiology. 75: 15-20.
- Kostaman, T. dan I.K. Sutama. 2006. Studi Motilitas Dan Daya Hidup Spermatozoa Kambing Boer Pada Pengencer Tris-Sitrat-Fruktosa. J. Sain Veteriner. 24 (1): 58-63.
- Kusumawati, E.D., A.T.N. Krisnaningsih, dan R.R. Romadlon. 2016. Kualitas Spermatozoa Semen Beku Sapi Simental dengan Suhu dan Lama Thawing yang Berbeda. Jurnal Ilmu-Ilmu Peternakan. 26(3): 38-41.
- Oyeyemi, M., M.O. Akusu dan O.E. OlaDavis. 2000. Effect of Successive Ejaculations on the Spermiogram of West African Dwarf Goats (Capra hircus L.). Jurnal Veterinarski Arhiv. 70(4): 215-221.
- Pamungkas, F. A., A. Batubara, dan Anwar. 2014. Kriopreservasi Spermatozoa Kambing Boer: Perbandingan Dua Bahan Pengencer terhadap Kualitas Post-thawing dan Kemampuan Fertilisasinya. Jurnal Ilmu Ternak dan Veteriner. 19: 130-137.
- Rizal, M. dan Herdis. 2008. Inseminasi Buatan Pada Domba. Jakarta: Rineka Cipta.
- Salim, M.A., T. Susilawati dan S. Wahyuningsih. 2012. Pengaruh Metode Thawing terhadap Kualitas Semen Beku Sapi Bali, Sapi Madura dan Sapi PO. Agripet. 12 (2): 14 -20.
- Samsudewa, D. 2008. Pengaruh Berbagai Metode Thawing terhadap Kualitas Semen Beku Sapi. Seminar Nasional Teknologi Peternakan dan Veteriner. Fakultas Peternakan, Universitas Diponegoro, Semarang.
- Setiadi, B., I. Sutama., P. Situmorang., Supriyati, U. Adiati., I. Budiarsana, dan Mulyawan. 2000. Evaluasi Karakteristik Semen Kambing Calon Bibit. Balai Penelitian Ternak. 5(2) : 74–87.
- Singh, M., R. T. Mollier, N. Pongener and P. R. Sharma. 2021. Artificial insemination in pig for enhancing the pig productivity in North Eastern Hill region. Indian Farming. 71 (2): 20 – 22.
- Susilawati, T. 2013. Pedoman Inseminasi Buatan pada Ternak. Malang: UB Press
- Susilawati, T.. 2011. Spermatology. UB Press: Malang.
- Suyadi, T. Susilawati dan N. Isnaini. 2004. Uji Coba Produksi Semen Beku Kambing Boer. Laporan Penelitian Kerjasama Ditjen Peternakan – Fakultas Peternakan Universitas Brawijaya Malang.
- Yulnawati dan M. A. Setiadi. 2005. Motilitas dan Keutuhan Membran Plasma Spermatozoa Epididimis Kucing selama Penyimpanan pada Suhu 4°C. J Med Vet. 21(3): 100-104
- Zelpina, E., B. Rosadi dan T. Sumarsono. 2012. Kualitas Spermatozoa Post thawing Dari Semen Beku Sapi Perah. Jurnal Ilmu-Ilmu Peternakan. 15 (2): 98-100.
References
Agustian, M.F., M.N. Ihsan dan N. Isnaini. 2014. Pengaruh Lama Simpan Semen Dengan Pengencer Tris Aminomethan Kuning Telur Pada Suhu Ruang Terhadap Kualitas Spermatozoa Kambing Boer. J. Ternak Tropika. 15 (2): 1-6.
Ansary, M.S., A. Bushra, Rakha and S. Akher. 2010. Effect of Straw Size and Thawing Time on Quality of Cryopreserved Buffalo (Bubalus bubalis) Semen. J Reproductive Biology. 11(1): 49-54.
Aprilina, N., S. Suharyati dan P.E. Santosa. 2014. Pengaruh Suhu Dan Lama Thawing di Dataran Rendah Terhadap Kualitas Semen Beku Sapi Simmental. Jurnal Ilmiah Peternakan Terpadu. 2(3): 96-102.
Datta, U., Sekar, M. C., Hembram, M. L., Dasgupta, R., 2009. Development of a New Methode to Preserve Caprine Cauda Epididymal Spermatozoa in situ at 10°C. Procedings. Departement of Veterinary Gynaecology & Obstetrics Faculty of Veterinary and Animal Sciences West Bengal University of Animal and Fishery Sciences. Kolkata West Bengal. India.
Fitrik dan N. Supartini. 2012. Pengaruh Suhu dan Lama Thawing terhadap Kualitas Spermatozoa Kambing Peranakan Etawa. Buana Sains. 12(1): 81-86.
Hoesni, F. 2013. Pengaruh Penggunaan Metode Thawing yang Berbeda terhadap Kualitas Spermatozoa Semen Sapi Perah Berpengencer Tris Sitrat Kuning Telur. Jurnal Ilmiah Universitas Batanghari Jambi. 13(4): 118-126.
Jamadi, A.S., E. Ahmad, M. Ansari and H. Kohram. 2017. Antioxidant effect of quercetin in an extender containing DMA or glycerol on freezing capacity of goat semen. Cryobiology. 75: 15-20.
Kostaman, T. dan I.K. Sutama. 2006. Studi Motilitas Dan Daya Hidup Spermatozoa Kambing Boer Pada Pengencer Tris-Sitrat-Fruktosa. J. Sain Veteriner. 24 (1): 58-63.
Kusumawati, E.D., A.T.N. Krisnaningsih, dan R.R. Romadlon. 2016. Kualitas Spermatozoa Semen Beku Sapi Simental dengan Suhu dan Lama Thawing yang Berbeda. Jurnal Ilmu-Ilmu Peternakan. 26(3): 38-41.
Oyeyemi, M., M.O. Akusu dan O.E. OlaDavis. 2000. Effect of Successive Ejaculations on the Spermiogram of West African Dwarf Goats (Capra hircus L.). Jurnal Veterinarski Arhiv. 70(4): 215-221.
Pamungkas, F. A., A. Batubara, dan Anwar. 2014. Kriopreservasi Spermatozoa Kambing Boer: Perbandingan Dua Bahan Pengencer terhadap Kualitas Post-thawing dan Kemampuan Fertilisasinya. Jurnal Ilmu Ternak dan Veteriner. 19: 130-137.
Rizal, M. dan Herdis. 2008. Inseminasi Buatan Pada Domba. Jakarta: Rineka Cipta.
Salim, M.A., T. Susilawati dan S. Wahyuningsih. 2012. Pengaruh Metode Thawing terhadap Kualitas Semen Beku Sapi Bali, Sapi Madura dan Sapi PO. Agripet. 12 (2): 14 -20.
Samsudewa, D. 2008. Pengaruh Berbagai Metode Thawing terhadap Kualitas Semen Beku Sapi. Seminar Nasional Teknologi Peternakan dan Veteriner. Fakultas Peternakan, Universitas Diponegoro, Semarang.
Setiadi, B., I. Sutama., P. Situmorang., Supriyati, U. Adiati., I. Budiarsana, dan Mulyawan. 2000. Evaluasi Karakteristik Semen Kambing Calon Bibit. Balai Penelitian Ternak. 5(2) : 74–87.
Singh, M., R. T. Mollier, N. Pongener and P. R. Sharma. 2021. Artificial insemination in pig for enhancing the pig productivity in North Eastern Hill region. Indian Farming. 71 (2): 20 – 22.
Susilawati, T. 2013. Pedoman Inseminasi Buatan pada Ternak. Malang: UB Press
Susilawati, T.. 2011. Spermatology. UB Press: Malang.
Suyadi, T. Susilawati dan N. Isnaini. 2004. Uji Coba Produksi Semen Beku Kambing Boer. Laporan Penelitian Kerjasama Ditjen Peternakan – Fakultas Peternakan Universitas Brawijaya Malang.
Yulnawati dan M. A. Setiadi. 2005. Motilitas dan Keutuhan Membran Plasma Spermatozoa Epididimis Kucing selama Penyimpanan pada Suhu 4°C. J Med Vet. 21(3): 100-104
Zelpina, E., B. Rosadi dan T. Sumarsono. 2012. Kualitas Spermatozoa Post thawing Dari Semen Beku Sapi Perah. Jurnal Ilmu-Ilmu Peternakan. 15 (2): 98-100.