Article Sidebar

Download
PDF
Statistic
Read Counter : 253 Download : 230

Downloads

Download data is not yet available.

Main Article Content

Abstract

This study aimed to determine the level of protection provided by supplementation of Azolla microphylla flour in broiler feed against the antibody titers profile of Avian Influenza and Newcastle Disease. The research was conducted over two months (July-August 2021) at the broiler unit of the Integrated Field Laboratory, Faculty of Agriculture, University of Lampung. The experimental design was a Completely Randomized Design with four treatments and five replications. Each replication consisted of five Cobb CP707 strain broilers, resulting in 100 broilers. Azolla microphylla flour supplementation in the feed was administered at different doses: P0=100% commercial feed (control); P1=97.5% commercial feed + 2.5% Azolla microphylla flour; P2=95% commercial feed + 5% Azolla microphylla flour; P3=92.5% commercial feed + 7.5% Azolla microphylla flour. Serum samples were collected from 30-day-old broilers, with one sample taken from each replication, totalling 20 samples. The samples were analyzed for Avian Influenza and Newcastle Disease antibody titers at AGRILab Vaksindo Laboratory using the Hemagglutination Inhibition test. Each treatment's antibody titer data for Avian Influenza and Newcastle Disease were tabulated and presented in histograms to facilitate descriptive analysis. Antibody titers (log2 X) of Avian Influenza 4.00±0.71 and Newcastle Disease 4.60±2.07 from P2 were included in the protection level based on the WOAH Standard. The study concluded that supplementing 5% Azolla microphylla flour in broiler feed increases the antibody titers against Avian Influenza and Newcastle Disease to protective levels.

Keywords

Antibody titer Avian influenza Azolla microphylla Newcastle disease Protection level

Article Details

License

Copyright (c) 2024 Jurnal Sain Peternakan Indonesia

Creative Commons License

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

The author who submits the manuscript must understand and agree that Jurnal Sain Peternakan Indonesia holds the copyrights published. Copyright includes rights to reproduce, distribute and sell every part of journal articles in all forms and media. This is a copyright transfer form (Download) signed by the corresponding author.

All articles published in Open Access will be immediately and permanently free for everyone to read and download. We are continuously working with our author communities to select the best choice of license options, currently being defined for this journal as follows:

• Creative Commons Attribution-ShareAlike (CC BY-SA)

Creative Commons License

Jurnal Sain Peternakan Indonesia is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

 

You are free to:

Share — copy and redistribute the material in any medium or format

Adapt — remix, transform, and build upon the material

for any purpose, even commercially.

The licensor cannot revoke these freedoms if you follow the license terms.

Author Biography

Ratna Ermawati, University of Lampung

Study Program of Animal Husbandry, Department of Animal Husbandry, Faculty of Agriculture, University of Lampung

How to Cite
Ermawati, R., Sirat, M. M. P., Hartono, M., Fathul, F., Hanafi, R., & Fauzi, T. A. (2024). Supplementation of Azolla microphylla Flour in Broiler Feed Increases Antibody Titers against Avian Influenza and Newcastle Disease. Jurnal Sain Peternakan Indonesia, 19(2), 59–67. Retrieved from https://ejournal.unib.ac.id/jspi/article/view/33899
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX

References

  1. Aiyer Harini, P., Ashok Kumar, H. G., Praveen Kumar, G., & Shivakumar, N. (2013). An overview of immunologic adjuvants - A review. Journal of Vaccines and Vaccination, 4(1), 167. https://doi.org/10.4172/2157-7560.1000167
  2. Balqis, U., Hambal, M., Mulyadi, Samadi, & Darmawi. (2011). Peningkatan Titer Antibodi Terhadap Avian Influenza Dalam Serum Ayam Petelur yang Divaksin Dengan Vaksin Komersial. Agripet, 11(1), 5–9. https://doi.org/10.17969/agripet.v11i1.647
  3. Bhatt, N., Tyagi, N., Chandra, R., Meena, D. C., & Prasad, C. K. (2021). Growth performance and nutrient digestibility of azolla pinnata feeding in sahiwal calves (bos indicus) by replacing protein content of concentrate with azolla pinnata during winter season. Indian Journal of Animal Research, 55(6), 663–668. https://doi.org/10.18805/ijar.B-4004
  4. Bhattacharyya, A., Shukla, P. K., Roy, D., & Shukla, M. (2016). Effect of Azolla Supplementation on Growth, Immunocompetence and Carcass Characteristics of Commercial Broilers. Journal of Animal Research, 6(5), 941. https://doi.org/10.5958/2277-940x.2016.00122.4
  5. Carlander, David. (2002). Avian IgY antibody : in vitro and in vivo [Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, ]. https://www.diva-portal.org/smash/record.jsf?pid=diva2%3A161296&dswid=-157
  6. Ekaningtias, M., Wuryastuty, H., & Wasito, W. (2017). Pendekatan Diagnosis Avian Influenza Virus dan Newcastle Disease Virus pada Kasus Lapangan Ayam Petelur: Imunopatologis Streptavidin Biotin. Jurnal Sain Veteriner, 35(1), 118–126. https://doi.org/10.22146/jsv.29299
  7. Ermawati, R., Sirat, M. M. P., Hartono, M., Fathul, F., & Farda, F. T. (2023). Efektivitas Suplementasi Tepung Azolla microphylla dalam Ransum Terhadap Bobot Badan, Total Protein Plasma Darah dan Total Cemaran Mikroba Karkas Broiler. Jurnal Ilmiah Peternakan Terpadu, 11(1), 65–81. https://doi.org/10.23960/jipt. v11i1.p65-81
  8. Hammond, Edward. (2009). Some intellectual property issues related to H5N1 Influenza viruses, research and vaccines. Third World Network. https://www.twn.my/title2/IPR/pdf/ipr12.pdf
  9. Khodijah, N. N. (2023). Deteksi Antibodi terhadap Virus Newcastle Disease pada Ayam Pedaging (Gallus domesticus Linnaeus, 1758) di Peternakan SMS Bekasi dengan Uji HA-HI [Fakultas Sains dan Teknologi Universitas Islam Negeri Syarif Hidayatullah ]. https://repository.uinjkt.ac.id/dspace/bitstream/123456789/66775/1/NADINI%20NUR%20KHODIJAH-FST.pdf
  10. Lehninger, A. L. (1997). Dasar-Dasar Biokimia (M. Thenawidjaja, Ed.; 1st ed.). Penerbit Erlangga.
  11. Murthy, T. N. K., Ashok, M., Thirumalesh, T., Umesh, B. U., & Nataraju, O. R. (2013). Effect of partial replacement of Azolla for concentrate supplement on lactating crossbred cows. Environment & Ecology, 31(2), 415–417. https://drive.google.com/file/d/1R_T1AEBtHI-n4N_LAJy1HYyFuVe6Sovz/view?usp=sharing.html
  12. Naghshi, H., Khojasteh, S., & Jafari, M. (2014). Investigation the effect of different levels of Azolla (Azolla pinnata) on performance and carcass characteristics of cobb broiler chicks. International Journal of Farming and Allied Sciences, 3(1), 45–49. https://ijfas.com/2014-3-1/
  13. Nguyen, D. C., Uyeki, T. M., Jadhao, S., Maines, T., Shaw, M., Matsuoka, Y., Smith, C., Rowe, T., Lu, X., Hall, H., Xu, X., Balish, A., Klimov, A., Tumpey, T. M., Swayne, D. E., Huynh, L. P. T., Nghiem, H. K., Nguyen, H. H. T., Hoang, L. T., … Katz, J. M. (2005). Isolation and Characterization of Avian Influenza Viruses, Including Highly Pathogenic H5N1, from Poultry in Live Bird Markets in Hanoi, Vietnam, in 2001. Journal of Virology, 79(7), 4201–4212. https://doi.org/10.1128/jvi.79.7.4201-4212.2005
  14. Nugrahawaty, T. (2002). Efek Berbagai Tingkat Protein Pakan Terhadap Titer Antibodi Ayam Petelur Jantan yang Divaksin ND (Newcastle Disease) [Skripsi, Universitas Airlangga]. https://repository.unair.ac.id/131194/
  15. Rai, R. B., Dhama, K., Damodaran, T., Ali, H., Rai, S., Singh, B., & Bhatt, P. (2012). Evaluation of Azolla (Azolla Pinnata) as a poultry feed and its role in poverty alleviation among landless people in northern plains of India. Veterinary Practitioner, 13(2), 250–254. https://www.vetpract.in/contents/vp-13-2-dec-2012
  16. Sanotharan, N., & Peramunagama, S. S. M. (2023, December 10). Use of Azolla as a sustainable protein and mineral supplement for livestock feed. Proceedings of the 79th Annual Sessions . https://doi.org/1391-023X
  17. Subowo. (2009). Imunobiologi Edisi 2. Sagung Seto. https://sagungseto.co.id/product/kedokteran/immunology/imunobiologi-ed-2/
  18. Subudhi, B. P. R., & Singh, P. K. (1978). Nutritive Value of the Water Fern Azolla pinnata for Chicks. Poultry Science, 57(2), 378–380. https://doi.org/10.3382/ps.0570378
  19. Tangendjaja, B. (2007). Inovasi Teknologi Pakan Menuju Kemandirian Usaha Ternak Unggas. Wartazoa, 17(1), 12–20. https://doi.org/10.14334/wartazoa.v17i1.886
  20. Wibawan, I. W. T. (2008). Pemanfaatan Telur Ayam sebagai Pabrik Biologis (Kajian Pustaka). Majalah Ilmiah Peternakan, 11(1), 36–41. https://ojs.unud.ac.id/index.php/mip/article/view/1763
  21. Wibawan, I. W. T., & Soejoedono, R. D. (2014). Intisari Imunologi Medis. PT. Penerbit IPB Press.
  22. WOAH. (2021a). WOAH Terrestrial Manual 2021 Chapter 3.3.4: Avian influenza (including infection with high pathogenicity avian influenza viruses). World Organisation for Animal Health. https://www.woah.org/fileadmin/Home/fr/Health_standards/tahm/3.03.04_AI.pdf
  23. WOAH. (2021b). WOAH Terrestrial Manual 2021 Chapter 3.3.14: Newcastle Disease (Infection with Newcastle Disease Virus). https://www.woah.org/fileadmin/Home/fr/Health_standards/tahm/3.03.14_NEWCASTLE_DIS.pdf
  24. Wulandari, E. C., Wahyuni, H. I., & Suthama, N. (2019). Kualitas Fisik Cangkang Telur Ayam Arab dengan Ransum Kombinasi Azolla microphylla dan Sumber Mineral Kalsium Berbeda. Jurnal Wahana Peternakan, 3(1), 21–25. https://doi.org/10.37090/jwputb.v3i1.113
  25. Yanshi, & Rahal, A. (2019). Azolla-Emerging Animal Feed. International Research Journal of Natural and Applied Sciences ISSN, 6(1), 1–12. https://aarf.asia/pages/applied2.php?p=Volume6,Issue1,January,2019