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Abstract

This study aims to identify the antibiotic resistance pattern of Escherichia coli in the broiler food chain at poultry slaughterhouses and outlets. The sampling method used purposive sampling. Each of the 30 cecum and carcass samples was obtained from a Poultry Slaughterhouse, and 40 samples from 1 retail (consisting of 5 outlets), which received supplies from the same Poultry Slaughterhouse, were isolated and identified as E. coli. The results showed that 57 isolates were positively identified as E. coli, and the sensitivity was tested for the Antibiotic Susceptibility Test using the disc diffusion method. Statistical analysis using the Chi-Square method and Fisher's Exact Test (p-value=0.01) showed a pattern of sensitivity of Ciprofloxacin (p=0.89), Colistin (p=0.07), Sulfamethoxazole (p=1.00), Chloramphenicol (p=0.67) and Meropenem (p= <0.001). The highest resistance was found in frozen carcass isolates at the outlet to Colistin (69%), and E. coli isolates from cecum at the poultry slaughterhouse showed resistance to Meropenem (67%). Furthermore, it was discovered that 35% of isolates were only resistant to one type of antibiotic. Resistance bacteria may spread and threaten consumers' health while they get carried from farm to retail.

Keywords

Antimicrobial resistance pattern AMR Escherichia coli broiler chicken English

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How to Cite
Emiliana, N., Khairani, N., Prayoga, R., Nurbiyanti, N., Sunandar, S., & Naipospos, T. S. P. (2024). Antibiotic Resistance Patterns of Escherichia coli Isolates in Broiler Chickens at Slaughterhouse and Retails. Jurnal Sain Peternakan Indonesia, 19(3), 117–123. https://doi.org/10.31186/jspi.id.19.3.117-123
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References

  1. Akond, M. A., S. Alam, S.M.R. Hassan, and M. Shirin. 2009. Antibiotic Resistance of Escherichia coli Isolated from Poultry and Poultry Environment of Bangladesh. American Journal of Environmental Sciences 5(1), 47–52.
  2. Besung, I N. K., I G.K. Suarjana, and K.P.G Tono. 2019. Resistensi Antibiotik pada Escherichia coli yang Diisolasi dari Ayam Petelur. Buletin Veteriner Udayana 11(1), 28–32.
  3. Bhunia, A. K. 2008. Foodborne Microbial Pathogens. 1st Edition. USA: Springer, New York, NY.
  4. Brooks, G. F, K. C. Caroll, J.S. Butel, and S.. A Morse. 2007. Jawetz, Melnick, & Adelberg’s Medical Microbiology. 24th ed. USA: The McGraw-Hill Companies.
  5. Cheney, T.E.A, R.P. Smith, J.P. Hutchinson, L.A. Brunton, G. Pritchard, and C.J. Teale. 2015. Cross-sectional Survey of Antibiotic Resistance in Escherichia coli Isolated from Diseased Farm Livestock in England and Wales. Epidemiology and Infection 143(12), 2653–59.
  6. Food and Agriculture Organization of the United Nations. 2019. Regional Antimicrobial Resistance Monitoring and Surveillance Guidelines. Volume 1, Monitoring and Surveillance of Antimicrobial Resistance in Bacteria from Healthy Food Animals Intended for Consumption. Bangkok: Food & Agriculture Organization of the United Nations.
  7. Indonesia Ministry of Health. 2017. Apoteker Ikut Atasi Masalah Resistensi Antimikroba Unpublished gov. Kementerian Kesehatan RI: Farmalkes. https://farmalkes.kemkes.go.id/2017/11/peningkatan-pelayanan-kefarmasian-dalam-pengendalian-resistensi-antimikroba-apoteker-ikut-atasi-masalah-resistensi-antimikroba/.
  8. Indonesian Ministry of Agriculture. 2017. Regulation of Indonesian Minister of Agriculture Number 14 Year 2017.
  9. Indonesian Ministry of Agriculture. 2023. Indonesian Veterinary Drug Index by the Indonesian Ministry of Agriculture. https://iohi.ditjenpkh.pertanian.go.id/iohi/filter?keyword=.
  10. Jeżak, K., and A. Kozajda. 2022. Occurrence and Spread of Antibiotic-Resistant Bacteria on Animal Farms and in their Vicinity in Poland and Ukraine—Review. Environmental Science and Pollution Research 29(7), 9533–59.
  11. Jufri, R. F. 2020. Microbial Isolation. Journal La Lifesci 1(1), 18–23.
  12. Kallau, N., I W. T. Wibawan, D. W. Lukman, and M. B. Sudarwanto. 2019. Studi Pemetaan Multi-Drug Resistant (MDR) Escherichia coli pada Peternakan Babi di Kota Kupang. Jurnal Kajian Veteriner 7(1), 70–79.
  13. Kousar, S., N. R., A. Javed, A. Hussain, M. Naeem, S. Masood, H. A. Ali, A. Manzoor, A. A. Khan, A. Akrem, F. Iqbal, A. Zulfiqar, M. B. Jamshaid, M. Waqas, A. Waseem, and M. Q. Saeed. 2021. Intensive Poultry Farming Practices Influence Antibiotic Resistance Profiles in Pseudomonas aeruginosa Inhabiting Nearby Soils. Infection and Drug Resistance Volume 14, 4511–16.
  14. EFSA BIOHAZ Panel (EFSA Panel on Biological Hazards), Koutsoumanis K, Allende A, Álvarez-Ordóñez A, Bolton D, Bover-Cid S, Chemaly M, Davies R, De Cesare A, Herman L, Hilbert F, Lindqvist R, Nauta M, Ru G, Simmons M, Skandamis P, Suffredini E, Argüello-Rodríguez H, Dohmen W, Magistrali CF, Padalino B, Tenhagen B-A, Threlfall J, García-Fierro R, Guerra B, Liébana E, Stella P and Peixe L, 2022. Scientific Opinion on the transmission of antimicrobial resistance (AMR) during animal transport. EFSA Journal 2022; 20(10):7586, 1-83 pp. https://doi.org/10.2903/j.efsa.2022.7586
  15. Kusumaningsih, A., and M. Sudarwanto. 2011. Infeksi Salmonella enteritidis pada Telur Ayam dan Manusia serta Resisten terhadap Antibiotik. Berita Biologi Jurnal-Ilmu-Ilmu Hayati 10(6), 771–79.
  16. Lestari, P. B., and T. W. Hartati. 2017. Mikrobiologi Berbasis Inkuiry. Cetakan I. Malang: Penerbit Gunung Samudera.
  17. Magnusson, U., A. Moodley, and K. Osbjer. 2021. Antimicrobial Resistance at the Livestock-Human Interface: Implications for Veterinary Services. Rev. Sci. Tech. Off. Int. Epiz 40(2), 1–19.
  18. Marshall, B. M., and S. B. Levy. 2011. Food Animals and Antimicrobials: Impacts on Human Health. Clinical Microbiology Reviews 24(4), 718–33.
  19. Murray, L. M., A. Hayes, J. Snape, B. Kasprzyk-Hordern, W. H. Gaze, and A. K. Murray. 2024. Co-selection for antibiotic resistance by environmental contaminants. npj Antimicrobials and Resistance 2(1), 9.
  20. Niasono, A. B., H. Latif, and T. Purnawarman. 2019. Resistensi Antibiotik Terhadap Bakteri Escherichia coli yang Diisolasi dari Peternakan Ayam Pedaging di Kabupaten Subang, Jawa Barat. Jurnal Veteriner 20(2), 187–95.
  21. Normaliska, R., M. B. Sudarwanto, and H. Latif. 2019. Pola Resistensi Antibiotik pada Escherichia coli Penghasil ESBL dari Sampel Lingkungan di RPH-R Kota Bogor. Acta Veterinaria Indonesiana 7(2), 42–48.
  22. Novianti, H. R., E. T. Marlina, and D. Z. Badruzzaman. 2021. Kajian Mikrobiologis Daging Ayam Giling yang dijual di Supermarket Wilayah Jatinangor. Jurnal Teknologi Hasil Peternakan 2(2), 82–94.
  23. Nurjanah, G. S., A. I. Cahyadi, and S. Windria. 2020. Escherichia coli Resistance to Various Kinds of Antibiotics in Animals and Humans: A Literature Study. Indonesia Medicus Veterinus 9(6), 970–83.
  24. Rorong, J. A., and W. F. Wilar. 2020. Keracunan Makanan Oleh Mikroba. Techno Science Journal 2(2), 47–60.
  25. Sani, R. A., J. A. Wagenaar, T. EHA Dinar, S. Sunandar, N. Nurbiyanti, I. Suandy, G. Pertela, E. J Jahja, B. Purwanto, CORNERSTONE group, I. M. V. Geijlswijk, and D. C. Speksnijder. 2023. The Comparison and Use of Tools for Quantification of Antimicrobial Use in Indonesian Broiler Farms. Frontiers in Veterinary Science 10, 1–12.
  26. Sasongko, H. 2014. Uji Resistensi Bakteri Escherichia Coli dari Sungai Boyong Kabupaten Sleman terhadap Antibiotik Amoksisilin, Kloramfenikol, Sulfametoxasol, dan Streptomisin. Jurnal Bioedukatika 2(1), 25.
  27. Spellberg, B., J. G. Bartlett, and D. N. Gilbert. 2013. The Future of Antibiotics and Resistance. New England Journal of Medicine 368(4), 299–302.
  28. World Health Organization. 2019. Critically Important Antimicrobials for Human Medicine. 6th rev. Geneva: World Health Organization.
  29. World Health Organization. 2023. Antimicrobial Resistance. Fact sheets of Antimicrobial Resistance.
  30. World Organisation for Animal Health. 2019. OIE List of Antimicrobial Agents of Veterinary Importance. Paris.