Main Article Content
Abstract
This study aimed to determine the effect of vitamin E supplementation on tris-egg yolk diluent using different quality fresh semen. The research material was ejaculated from six Simmental bulls and then classified into standard quality (SQ) and low quality (LQ) according to progressive motility. The research method was experimental with a randomized factorial design (2X5), with the group by fresh semen with 3 replications. The first factor was the quality of fresh semen, while the second factor was the level of vitamin E addition (B0: 0mg/ml; B1: 0.5 mg/ml; B2: 1 mg/ml; B3: 1.5 mg/ml; and B4: 2 mg/ml). After thawing, the observation parameters were motility, viability, abnormality, plasma membrane integrity, and MDA levels. The result shows that the quality of fresh semen has a very significant effect (p<0.01) on the motility of frozen semen (66.28±8.66%) and a significant impact (p<0.05) on the plasma membrane integrity (91.31±2.87%). The use of vitamin E at 1 mg/ml showed the best results on motility (66.11±7.64%), viability (70.19±12.13), abnormality (12.00±3.13), and MDA (2.11±0.77). This study concludes that adding vitamin E to the diluent can increase motility, viability, and plasma membrane integrity while reducing abnormality and MDA levels. Low-quality fresh semen can be processed into frozen semen after adding vitamin E. The best level of vitamin E addition is 1 mg/ml.
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Effect of Vitamin E Supplementation In Diluent on Fertility Parameters of Different Quality Fresh Bull Sperm. (2025). Jurnal Sain Peternakan Indonesia, 20(1), 19–28. https://doi.org/10.31186/jspi.id.20.1.19-28
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Ramazani, N., Mahd Gharebagh, F., Soleimanzadeh, A., Arslan, H. O., Keles, E., Gradinarska-Yanakieva, D. G., Arslan-Acaröz, D., Zhandi, M., Baran, A., Ayen, E., & Dinç, D. A. (2023). Reducing oxidative stress by κ-carrageenan and C60HyFn: The post-thaw quality and antioxidant status of Azari water buffalo bull semen. Cryobiology, 111(April), 104–112. https://doi.org/10.1016/j.cryobiol.2023.04.003
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Tanga, B. M., Qamar, A. Y., Raza, S., & Bang, S. (2021). Semen evaluation : methodological advancements in sperm quality-specific fertility assessment — A review. 34(8), 1253–1270.
Ullah, Z., Khan, H., Hussain, S. M., Tunio, M. T., Dilshad, S. M. R., Gohar, A., Zahid, H., & Ali, A. (2019). Enhancement of extender excellence of frozen bull semen using α-tocopherol as an antioxidant. Acta Scientiae Veterinariae, 47(1), 1–6. https://doi.org/10.22456/1679-9216.90447
Upadhyay, V. R., Ramesh, V., Dewry, R. K., Yadav, D. K., & Ponraj, P. (2022). Bimodal interplay of reactive oxygen and nitrogen species in physiology and pathophysiology of bovine sperm function. Theriogenology, 187, 82–94. https://doi.org/10.1016/j.theriogenology.2022.04.024
Wang, M., Wu, S., Yang, B., Ye, M., Tan, J., Zan, L., & Yang, W. (2023). Grape Seed Proanthocyanidins Improve the Quality of Fresh and Cryopreserved Semen in Bulls. Animals, 13(17). https://doi.org/10.3390/ani13172781
Wijayanti, A., Suprayogi, T. W., Prastiya, R. A., Hernawati, T., Sardjito, T., Saputro, A. L., Amaliya, A., & Sulistyowati, D. (2023). Effect of Addition of Green Tea Extract (Camellia sinensis) in Egg Yolk Tris Diluter on Spermatozoa Quality in Bali Cattle (Bos sondaicus) After Freezing. Jurnal Medik Veteriner, 6(1), 66–74. https://doi.org/10.20473/jmv.vol6.iss1.2023.66-74
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