Article Sidebar

Submitted
March 3, 2020
Accepted
May 11, 2020
Published
May 30, 2020
Download
PDF
Statistic
Read Counter : 241 Download : 2423

Main Article Content

Abstract

Adlay is a minor cereal which utilization is not optimal in Indonesia. Adlay grains have natural antioxidants in the form of phenolic compounds and flavonoids found in bran, husk, and testa. Purpose of this study was to determine the type of  adlay bran extraction solvent that produced the highest antioxidant activity. The study used an experimental method with a randomized block design (RBD) consisting of 3 treatments that were repeated 4 times, namely extraction with (1) ethanol solvent; (2) n-hexane solvent; and (3) ethanol solvent then the waste is extracted again with hexane solvent. The extract obtained was analyzed for its antioxidant activity by DPPH method, total phenol, total tocopherol and yield. The results showed extracts from ethanol solvents produced the highest antioxidant activity where IC50 values were 771.73 ppm, but categorized as antioxidants with very weak activity. The extraction of adlay bran with ethanol solvent resulted in a yield of 1.91%, total phenol of 0.92%, and total tocopherol 0.09 mg / mL

 

Article Details

License

Authors who publish in this journal agree with the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
  4. This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
How to Cite
Tensiska, T., Nurhadi, B., Wulandari, E., & Ratri, Y. A. L. (2020). ANTIOXIDANT ACTIVITIY OF ADLAY EXTRACT (Coix lachryma-jobi L.) WITH DIFFERENT SOLVENT. Jurnal Agroindustri, 10(1), 1–11. https://doi.org/10.31186/j.agroindustri.10.1.1-11
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX

References

  1. Ascociation of Analytical Chemists (AOAC). 2012. International Official Method.
  2. Brindzova, L., M. Zalibera, T. Jakubík, M. Mikulášová, M. Takácsová, S. Mošovská dan P. Rapta. 2009. Antimutagenic And Radical Scavenging Activity Of Wheat Bran. Cereal Research Communications, 37 (1), 45–55.
  3. Brown, T. A. 1995. Gene cloning: an introduction/by TA Brown.
  4. Duke, J. A., dan E. S. Ayensu. 1985. Medicinal Plants of China. . Reference Publications Inc. Algonac. MI.
  5. Fessenden, R.J. dan J.S. Fessenden. 1983. Kimia Organik. Edisi Kedua. Penerbit Erlangga, Jakarta.
  6. Gong, E. S., S.J. Luo, T. Li, C.M. Liu, G.W. Zhang, J. Chen, R.H. Liu. 2017. Phytochemical Profiles And Antioxidant Activity of Brown Rice Varieties. Food Chemistry, 227: 432–443.
  7. Hadipernata, M., W. Supartono dan M.A.F. Falah. 2012. Proses Stabilisasi Dedak Padi (Oryza sativa L) Menggunakan Radiasi Far Infra Red (FIR) Sebagai Bahan Baku Minyak Pangan. Jurnal Aplikasi Teknologi Pangan, 1(4):103-107
  8. Harborne, J. B. 1996. Metode Fitokimia, Penuntun Cara Modern Menganalisis Tumbuhan, translated by K. Padmawirata. dan I. Soediro. ITB Press, Bandung.
  9. Harborne, J. B. 1987. Metode Fitokimia: Penuntun Cara Modern Menganalisis Tumbuhan. Penerbit ITB, Bandung.
  10. He, C., Z. Li, H. Liu, H. Zhang, L. Wang and H. Chen. 2019. Chemical Compositions and Antioxidant Activity of Adlay Seed (Coixlachryma-jobi L.) Oil Extracted from Four Main Producing Areas in China. Food Chemistry 85 (1): 123-131
  11. Herawati, D. dan L.N. Sumarto. 2012. Cara Produksi Simplisia yang Baik. Seafast Center, Bogor.
  12. Houghton, J. P. dan A. Raman. 1998. Handbook for the Fractionation of Natural Extracts. Chapman and Hall, London.
  13. Hsia, S.-M., Y.H. Kuo, W. Chiang dan P.S. Wang. 2008. Effects of Adlay Hull Extracts on Uterine Contraction and Ca2+ Mobilization In The Rat. American Journal of Physiology-Endocrinology and Metabolism, 295(3): E719–E726.
  14. Huang, D.W., C.P. Chung, Y.H. Kuo, Y.L. Lin dan W. Chiang. 2009a. Identification of Compounds In Adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) Seed Hull Extracts That Inhibit Lipopolysaccharide-Induced Inflammation In RAW 264.7 Macrophages. Journal of Agricultural and Food Chemistry, 57(22): 10651–10657.
  15. Huang, D.W., Y.H. Kuo, F.Y. Lin, Y.L. Lin dan W. Chiang. 2009b. Effect of Adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) Testa And Its Phenolic Components On Cu2+-Treated Low-Density Lipoprotein (LDL) Oxidation And Lipopolysaccharide (LPS)-Induced Inflammation In RAW 264.7 Macrophages. Journal of Agricultural and Food Chemistry, 57(6): 2259–2266.
  16. Huang, D.W., C.H. Wu, C.K. Shih, C.Y. Liu, P.H. Shih, T.M. Shieh dan S.M. Hsia. 2014. Application Of The Solvent Extraction Technique To Investigation Of The Anti-Inflammatory Activity Of Adlay Bran. Food Chemistry, 145: 445–453.
  17. Huliselan, Y. M. (2015). Aktivitas Antioksidan Ekstrak Etanol, Etil Asetat, dan n-Heksan dari Daun Sesewanua (Clerodendron squamatum Vahl.). Pharmacon, 4(3): 155–163.
  18. Kuo, C.C., W. Chiang, G.P. Liu, Y.L. Chien, J.Y. Chang, C.K. Lee, dan Y.H. Kuo. 2002. 2,2‘-Diphenyl-1-picrylhydrazyl Radical-Scavenging Active Components From Adlay (Coix lachryma-jobi L. Var. ma-yuen Stapf) Hulls. Journal of Agricultural and Food Chemistry, 50(21): 5850–5855.
  19. Lehninger, A.L. 1995. Dasar-Dasar Biokimia. Penerjemah: M. Thenawijaya. Penerbit Erlangga, Jakarta.
  20. Molyneux, P. 2004. The Use Of The Stable Free Radical diphenylpicrylhydrazyl (DPPH) For Estimating Antioxidant Activity. Songklanakarin J. Sci. Technol, 26(2): 211–219.
  21. Mumpuni, P. D. dan F. Ayustaningwarno. 2013. Analisis Kadar Tokoferol,?-Oryzanol Dan ?-Karoten Serta Aktivitas Antioksidan Minyak Bekatul Kasar. Journal of NutritionCollege, 2(3): 350-357.
  22. Povilaitis, D., V. Šulni?t?, P.R. Venskutonis dan V. Kraujalien?. 2015. Antioxidant Properties Of Wheat And Rye Bran Extracts Obtained By Pressurized Liquid Extraction With Different Solvents. Journal of Cereal Science, 62: 117–123.
  23. Rodriguez?Saona, L. E., M.M. Giusti, R.W. Durstdan R.E. Wrolstad. 2001. Development And Process Optimization Of Red Radish Concentrate Extract As Potential Natural Red Colorant. Journal of Food Processing and Preservation, 25(3): 165–182.
  24. Romadanu, R., S. Hanggita dan S.D. Lestari. 2014. Pengujian Aktivitas Antioksidan Ekstrak Bunga Lotus (Nelumbo nucifera). Jurnal FishtecH, 3(1): 1–7.
  25. Shahidi, F. dan M. Naczk. 1995. Food Phenolics. Technomic pub. Co. Inc. Lancester-Basel.
  26. Shahwar, D., N. Ahmad, S. Ullah dan M.A. Raza. 2010. Antioxidant Activities Of The Selected Plants From The Family Euphorbiaceae, Lauraceae, Malvaceae and Balsaminaceae. African Journal of Biotechnology, 9(7): 1086–1096.
  27. Sudarmadji, S., Suhardi dan B. Haryono. 1989. Analisa Bahan Makanan Dan Pertanian. Liberty, Yogyakarta.
  28. Syahbirin, G., I. Batubara, T. Setiawati dan L. Nulhakim. 2005. Senyawa Aktif Daun Picung (Pangium edule Reinw.) Sebagai Insektisida Botani Terhadap Ulat Gerayak (Spodoptera litura F.)(Lepidoptera: Noctuidae). Prosiding Simposium Nasional Kimia Bahan Alam XV, 56–66.
  29. Tambe, V. D., dan R.S. Bhambar. 2014. Estimation Of Total Phenol, Tannin, Alkaloid And Flavonoid In Hibiscus tiliaceus Linn. Wood Extracts. Journal of Pharmacognosy and Phytochemistry, 2(4): 41–47.
  30. Barberán, F. A. T. dan J.C. Espín. 2001. Phenolic Compounds And Related Enzymes As Determinants Of Quality In Fruits And Vegetables. Journal of the Science of Food and Agriculture, 81(9): 853–876.
  31. Wang, L., J. Chen dan H. Xie. 2013. Phytochemical Profiles and Antioxidant Activity of Adlay Varities. Journal of Agricultural and Food Chemistry 61 (21):5103-13
  32. Wang, Jiajing, L. Liu, T. Ball, L. Yu, Y. Li dan F. Xing. 2016. Revealing a 5,000-y-old beer recipe in China. Proceedings of the National Academy of Sciences, 113(23): 6444–6448.
  33. Wang, J., B. Sun, Y. Cao, Y. Tian dan X. Li. 2008. Optimisation Of Ultrasound-Assisted Extraction Of Phenolic Compounds From Wheat Bran. Food Chemistry, 106(2): 804–810.
  34. Wang, L., C. Chen, A. Su, Y. Zhang, J. Yuan dan X. Ju. 2016. Structural Characterization Of Phenolic Compounds And Antioxidant Activity Of The Phenolic-Rich Fraction From Defatted Adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) Seed Meal. Food Chemistry, 196: 509–517.
  35. Weber, M., M. Weber dan L. Pilato. 2010. Phenolic Resins: A Century Of Progress. Berlin and Heidelberg, SpringerVerlage.
  36. Woo, K. S. 2004. Use of bee venom and propolis for apitherapy in Korea. 7. Asian Apicultural Association Conference and 10. BEENET Symposium and Technofora,, College, Laguna (Philippines), 23-27 Feb 2004. UPLB-BP.
  37. Xu, L., P. Wang, B. Ali, and Y. Na. 2017. Changes of the Phenolic Compounds and Antioxidant Activities in Germinated Adlay Seeds. Journal of the Science of Food and Angriculture 97 (12): 4227-4234
  38. Yang, Q., M. Zhao, dan L. Lin. 2016. Adsorption And Desorption Characteristics Of Adlay Bran Free Phenolics On Macroporous Resins. Food Chemistry, 194: 900–907.
  39. Yuliani, S., N. Harimurti dan S.S. Yuliani. 2019. Pengaruh laju alir umpan dan suhu inlet spray drying pada karakteristik mikrokapsul oleoresin jahe. Jurnal Penelitian Pascapanen Pertanian, 4(1): 18–26.