Main Article Content
Abstract
White egg contains the most significant protein source that has been explored to give more benefit for health purposes. This study evaluated the potential of antibacterial and antioxidant activity of white egg protein hydrolysate extracted from Indonesian avian eggs. The avian egg materials used local Indonesian chicken eggs (kampung chicken), chicken breed eggs, duck eggs, and muscovy duck eggs. The white egg was extracted by organic solvent and produced protein extracted which was dominated by ovalbumin and ovotransferrin. The protein extracted was hydrolyzed by pepsin and then powdered using a freeze dryer. Antibacterial and antioxidant activity was evaluated by agar well diffusion technique and 2,2-Diphenyl-1-picryl hydroxyl (DPPH) radical scavenging method. All samples showed antimicrobial activity against Salmonella sp., Escherichia coli, Staphylococcus aureus, Bacillus cereus, and Bacillus subtilis with a concentration of protein hydrolysate at 40 mg/mL. The antioxidant activity between avian species was different and dose-dependent. Protein hydrolysate extracted from duck white egg exhibited the highest radical scavenging activity (65%) at 10 mg/mL concentration. However, all white egg protein hydrolysate extracted from Indonesian avian eggs can potentially help reduce oxidation and microbial deterioration.
Keywords
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References
- Abeyrathne, E. D. N. S., H. Y. Lee, J. S. Ham, and D. U. Ahn. 2013. Separation of ovotransferrin from chicken egg white without using organic solvents. Poult Sci. 92(4):1091–1097.
- Abeyrathne, E. D. N. S., H. Y. Lee, C. Jo, K. C. Nam, and D. U. Ahn. 2014. Enzymatic hydrolysis of ovalbumin and the functional properties of the hydrolysates. Poult Sci. 93(10):2678–2686.
- Al-Mohammadi, A. R., A. Osman, G. Enan, S. Abdel-Shafi, M. El-Nemer, M. Sitohy, and M. A. Taha. 2020. Powerful antibacterial peptides from egg albumin hydrolysates. Antibiotics. 9(12):1–21.
- Benedé, S., and E. Molina. 2020. Chicken egg proteins and derived peptides with antioxidant properties. Foods. 9(735).
- Cruz, V. L., J. Ramos, J. Martinez-Salazar, M. Montalban-Lopez, and M. Maqueda. 2021. The Role of Key Amino Acids in the Antimicrobial Mechanism of a Bacteriocin Model Revealed by Molecular Simulations. J Chem Inf Model. 61(12):6066–6078.
- Garcés-Rimón, M., C. González, J. A. Uranga, V. López-Miranda, R. López-Fandiño, and M. Miguel. 2016. Pepsin egg white hydrolysate ameliorates obesity-related oxidative stress, inflammation and steatosis in Zucker fatty rats. PLoS One. 11(3).
- Giansanti, F., L. Leboffe, F. Angelucci, and G. Antonini. 2015. The nutraceutical properties of ovotransferrin and its potential utilization as a functional food. Nutrients. 7(11):9105–9115.
- Jahandideh, F., K. Majumder, S. Chakrabarti, J. S. Morton, S. Panahi, S. Kaufman, S. T. Davidge, and J. Wu. 2014. Beneficial Effects of SimulatedGastro-Intestinal Digests of Fried Egg andIts Fractions on Blood Pressure, PlasmaLipids and Oxidative Stress inSpontaneously Hypertensive Rats. PLoS One. :1–21.
- Jeevithan, E., B. Bao, J. Zhang, S. Hong, and W. Wu. 2015. Purification, characterization and antioxidant properties of low molecular weight collagenous polypeptide (37 kDa) prepared from whale shark cartilage (Rhincodon typus). J Food Sci Technol. 52(10):6312–6322.
- Kim, J., S. H. Moon, D. U. Ahn, H. D. Paik, and E. Park. 2012. Antioxidant effects of ovotransferrin and its hydrolysates. Poult Sci. 91(11):2747–2754.
- Ko, K. Y., A. F. Mendoncam, H. Ismail, and D. U. Ahn. 2009. Ethylenediaminetetraacetate and lysozyme improves antimicrobial activities of ovotransferrin against Escherichia coli O157:H7. Poult Sci. 88(2):406–414.
- Lee, J. H., S. H. Moon, H. S. Kim, E. Park, D. U. Ahn, and H. D. Paik. 2017. Antioxidant and anticancer effects of functional peptides from ovotransferrin hydrolysates. J Sci Food Agric. 97(14):4857–4864.
- Lee, J. H., and H. D. Paik. 2019. Anticancer and immunomodulatory activity of egg proteins and peptides: a review. Poult Sci. 98(12):6505–6516.
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- Moon, S. H., J. H. Lee, Y. J. Lee, J. Y. Paik, D. U. Ahn, and H.-D. Paik. 2012. Antioxidant, Antimicrobial, and Cytotoxic Activities of Ovotransferrin from Egg White. Korean J Food Sci Anim Resour. 32(5):612–617.
- Nariah, N., A. Mohamad Legowo, E. Abustam, and A. Hintono. 2015. Antioxidant and Antihypertensive Activity Egg White Powder Produced by Pan Drying at Different Temperature and Drying Time. Journal Ilmu dan Teknologi Peternakan. 4(1).
- Nasution, S., E. Kusumaningtyas, D. N. Faridah, and H. Kusumaningrum. 2018. Lysozyme from Chicken Egg White as an Antibacterial Agent. Wartazoa. 28(4):175–188.
- Otte, J., M. Abdel-Hamid, and A. Osman. 2015. Comparative assessment of peptide concentration in milk protein hydrolysates and fractions. International Journal of Dairy Science. 10(5):228–235.
- Pellegrini, A., A. J. Hülsmeier, P. Hunziker, and U. Thomas. 2004. Proteolytic fragments of ovalbumin display antimicrobial activity. Biochimica et Biophysica Acta . 1672(2):76–85.
- Rathnapala, E. C. N., D. U. Ahn, and S. Abeyrathne. 2021. Functional properties of ovotransferrin from chicken egg white and its derived peptides: a review. Food Sci Biotechnol. 30(5):619–630.
- Sun, S., H. Niu, T. Yang, Q. Lin, F. Luo, and M. Ma. 2014. Antioxidant and anti-fatigue activities of egg white peptides prepared by pepsin digestion. J Sci Food Agric. 94(15):3195–3200.
- Wellman-Labadie, O., J. Picman, and M. T. Hincke. 2008. Comparative antibacterial activity of avian egg white protein extracts. Br Poult Sci. 49(2):125–132.
References
Abeyrathne, E. D. N. S., H. Y. Lee, J. S. Ham, and D. U. Ahn. 2013. Separation of ovotransferrin from chicken egg white without using organic solvents. Poult Sci. 92(4):1091–1097.
Abeyrathne, E. D. N. S., H. Y. Lee, C. Jo, K. C. Nam, and D. U. Ahn. 2014. Enzymatic hydrolysis of ovalbumin and the functional properties of the hydrolysates. Poult Sci. 93(10):2678–2686.
Al-Mohammadi, A. R., A. Osman, G. Enan, S. Abdel-Shafi, M. El-Nemer, M. Sitohy, and M. A. Taha. 2020. Powerful antibacterial peptides from egg albumin hydrolysates. Antibiotics. 9(12):1–21.
Benedé, S., and E. Molina. 2020. Chicken egg proteins and derived peptides with antioxidant properties. Foods. 9(735).
Cruz, V. L., J. Ramos, J. Martinez-Salazar, M. Montalban-Lopez, and M. Maqueda. 2021. The Role of Key Amino Acids in the Antimicrobial Mechanism of a Bacteriocin Model Revealed by Molecular Simulations. J Chem Inf Model. 61(12):6066–6078.
Garcés-Rimón, M., C. González, J. A. Uranga, V. López-Miranda, R. López-Fandiño, and M. Miguel. 2016. Pepsin egg white hydrolysate ameliorates obesity-related oxidative stress, inflammation and steatosis in Zucker fatty rats. PLoS One. 11(3).
Giansanti, F., L. Leboffe, F. Angelucci, and G. Antonini. 2015. The nutraceutical properties of ovotransferrin and its potential utilization as a functional food. Nutrients. 7(11):9105–9115.
Jahandideh, F., K. Majumder, S. Chakrabarti, J. S. Morton, S. Panahi, S. Kaufman, S. T. Davidge, and J. Wu. 2014. Beneficial Effects of SimulatedGastro-Intestinal Digests of Fried Egg andIts Fractions on Blood Pressure, PlasmaLipids and Oxidative Stress inSpontaneously Hypertensive Rats. PLoS One. :1–21.
Jeevithan, E., B. Bao, J. Zhang, S. Hong, and W. Wu. 2015. Purification, characterization and antioxidant properties of low molecular weight collagenous polypeptide (37 kDa) prepared from whale shark cartilage (Rhincodon typus). J Food Sci Technol. 52(10):6312–6322.
Kim, J., S. H. Moon, D. U. Ahn, H. D. Paik, and E. Park. 2012. Antioxidant effects of ovotransferrin and its hydrolysates. Poult Sci. 91(11):2747–2754.
Ko, K. Y., A. F. Mendoncam, H. Ismail, and D. U. Ahn. 2009. Ethylenediaminetetraacetate and lysozyme improves antimicrobial activities of ovotransferrin against Escherichia coli O157:H7. Poult Sci. 88(2):406–414.
Lee, J. H., S. H. Moon, H. S. Kim, E. Park, D. U. Ahn, and H. D. Paik. 2017. Antioxidant and anticancer effects of functional peptides from ovotransferrin hydrolysates. J Sci Food Agric. 97(14):4857–4864.
Lee, J. H., and H. D. Paik. 2019. Anticancer and immunomodulatory activity of egg proteins and peptides: a review. Poult Sci. 98(12):6505–6516.
Lin, S., Y. Jin, M. Liu, Y. Yang, M. Zhang, Y. Guo, G. Jones, J. Liu, and Y. Yin. 2013. Research on the preparation of antioxidant peptides derived from egg white with assisting of high-intensity pulsed electric field. Food Chem. 139(1–4):300–306.
Miguel, M., M. A. Manso, R. López-Fandiño, and M. Ramos. 2005. Comparative study of egg white proteins from different species by chromatographic and electrophoretic methods. European Food Research and Technology. 221(3–4):542–546.
Moon, S. H., J. H. Lee, Y. J. Lee, J. Y. Paik, D. U. Ahn, and H.-D. Paik. 2012. Antioxidant, Antimicrobial, and Cytotoxic Activities of Ovotransferrin from Egg White. Korean J Food Sci Anim Resour. 32(5):612–617.
Nariah, N., A. Mohamad Legowo, E. Abustam, and A. Hintono. 2015. Antioxidant and Antihypertensive Activity Egg White Powder Produced by Pan Drying at Different Temperature and Drying Time. Journal Ilmu dan Teknologi Peternakan. 4(1).
Nasution, S., E. Kusumaningtyas, D. N. Faridah, and H. Kusumaningrum. 2018. Lysozyme from Chicken Egg White as an Antibacterial Agent. Wartazoa. 28(4):175–188.
Otte, J., M. Abdel-Hamid, and A. Osman. 2015. Comparative assessment of peptide concentration in milk protein hydrolysates and fractions. International Journal of Dairy Science. 10(5):228–235.
Pellegrini, A., A. J. Hülsmeier, P. Hunziker, and U. Thomas. 2004. Proteolytic fragments of ovalbumin display antimicrobial activity. Biochimica et Biophysica Acta . 1672(2):76–85.
Rathnapala, E. C. N., D. U. Ahn, and S. Abeyrathne. 2021. Functional properties of ovotransferrin from chicken egg white and its derived peptides: a review. Food Sci Biotechnol. 30(5):619–630.
Sun, S., H. Niu, T. Yang, Q. Lin, F. Luo, and M. Ma. 2014. Antioxidant and anti-fatigue activities of egg white peptides prepared by pepsin digestion. J Sci Food Agric. 94(15):3195–3200.
Wellman-Labadie, O., J. Picman, and M. T. Hincke. 2008. Comparative antibacterial activity of avian egg white protein extracts. Br Poult Sci. 49(2):125–132.