Main Article Content
Abstract
This study aimed to analyze the genetic diversity of the sambar deer (Cervus unicolor) population through mitochondrial D-loop DNA sequencing. The findings provide valuable insights into the conservation status of this vulnerable species in the region and inform future management strategies for Cervus unicolor. Blood samples from 10 individual sambar deer, consisting of 8 females and two male deer, were amplified using PCR and then sequenced. Data analysis of the genetic diversity of sambar deer was carried out using the genome sequencing method from NCBI, Bioedit 7.7.1 software, DNAsp 5.1, and MEGA 11 Software. The results of this study were that the DNA concentration test in sambar deer had an average of 12.375 ng/uL, with an average DNA purity test = 1.34, with 10 samples divided into 3 haplotypes. The level of genetic diversity of sambar deer from all samples is π = 0.01745 ± 0.00380, and haplotype diversity of Hd = 1,000 ± 0.045. Based on the phylogenetic tree, there are two parts: the Asian and Kalimantan regions. The conclusion of the current study showed that sequencing analysis of Sambar deer shows relatively high diversity and is a reasonable basis for performance selection and development of modern Sambar deer breeding.
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References
- Agisimanto, D., Supriyanto, A. 2007. Keragaman Genetik Pamelo Indonesia Berdasarkan Primer Random Amplified Polymorphic DNA. Jurnal Hortikultura, 17(1), 1–7.
- Ando, S., Komiyama, T., Sudo, M., Higaki, Y., Ishida, K., Costello, J. T., & Katayama, K. 2020. The interactive effects of acute exercise and hypoxia on cognitive performance: A narrative review. Scandinavian Journal of Medicine and Science in Sports, 30(3), 384–398. https://doi.org/10.1111/sms.13573
- Aulia, S. L., Suwignyo, R. A., & Hasmeda, M. 2021. Optimasi Suhu Annealing untuk Amplifikasi Dna Padi Hasil Persilangan Varietas Tahan Terendam dengan Metode Polymerase Chain Reaction. Sainmatika: Jurnal Ilmiah Matematika Dan Ilmu Pengetahuan Alam, 18(1), 44. https://doi.org/10.31851/sainmatika.v17i3.5805
- Avise, J. C., Arnold, J., Ball, R. M., Bermingham, E., Lamb, T., Neigel, J. E., Reeb, C. A., & Saunders, N. C. 1987. INTRASPECIFIC PHYLOGEOGRAPHY: The Mitochondrial DNA Bridge Between Population Genetics and Systematics. Annual Review of Ecology and Systematics, 18(1), 489–522. https://doi.org/10.1146/annurev.es.18.110187.002421
- Balakrishnan, C. N., Monfort, S. L., Gaur, A., Singh, L., & Sorenson, M. D. 2003. Phylogeography and conservation genetics of Eld’s deer (Cervus eldi). Molecular Ecology, 12(1), 1–10. https://doi.org/10.1046/j.1365-294X.2003.01751.x
- Dauzery, E., & Randi, E. 1997. The mitochondrial control region of Cervidae: Evolutionary patterns and phylogenetic content. Molecular Biology and Evolution, 14(11), 1154–1166. https://doi.org/10.1093/oxfordjournals.molbev.a025725
- Ghazi, M. G., Hussain, S. A., & Gupta, S. K. 2021. Detection of 40 bp tandem repeat motif and associated insertions and deletions (INDEL) in the mitochondrial DNA control region of Sambar deer (Rusa unicolor). Molecular Biology Reports, 48(5), 4129–4135. https://doi.org/10.1007/s11033-021-06426-6
- Hasibuan, F. E. B., Mantiri, F. R., & Rumende, R. R. . 2017. KAJIAN VARIASI SEKUNES INTRASPESIES DAN FILOGENETIK MONYET HITAM SULAWESI (Macaca nigra) DENGAN MENGGUNAKAN GEN COI. Jurnal Ilmiah Sains, 17(1), 59–67. https://doi.org/10.35799/jis.17.1.2017.15558
- Hisheh, S., Westerman, M., & Schmitt, L. H. 1998. Biogeography of the Indonesian archipelago: Mitochondrial DNA variation in the fruit bat, Eonycteris spelaea. Biological Journal of the Linnean Society, 65(3), 329–345. https://doi.org/10.1006/bijl.1998.0244
- Hobbs, J. P. A., Frisch, A. J., Ford, B. M., Thums, M., Saenz-Agudelo, P., Furby, K. A., & Berumen, M. L. 2013. Taxonomic, Spatial and Temporal Patterns of Bleaching in Anemones Inhabited by Anemonefishes. PLoS ONE, 8(8), 1–12. https://doi.org/10.1371/journal.pone.0070966
- Hu, J., Fang, S. G., & Wan, Q. H. 2006. Genetic diversity of Chinese water deer (Hydropotes inermis inermis): Implications for conservation. Biochemical Genetics, 44(3–4), 161–172. https://doi.org/10.1007/s10528-006-9020-7
- Liu, B., Tao, W., Feng, D., Wang, Y., Heizatuola, N., Ahemetbai, T., & Wu, W. 2022. Revealing Genetic Diversity and Population Structure of Endangered Altay White-Headed Cattle Population Using 100 k SNP Markers. Animals, 12(22), 1–17. https://doi.org/10.3390/ani12223214
- Liu, R. Y., Lei, C. Z., Liu, S. H., & Yang, G. S. 2007. Genetic diversity and origin of Chinese domestic goats revealed by complete mtDNA D-loop sequence variation. Asian-Australasian Journal of Animal Sciences, 20(2), 178–183. https://doi.org/10.5713/ajas.2007.178
- Liu, R. Y., Yang, G. S., & Lei, C. Z. 2006. The genetic diversity of mtDNA D-loop and the origin of Chinese goats. Acta Genetica Sinica, 33(5), 420–428. https://doi.org/10.1016/S0379-4172(06)60069-3
- Martins, R. F., Schmidt, A., Lenz, D., Wilting, A., & Fickel, J. 2018. Human-mediated introduction of introgressed deer across Wallace’s line: Historical biogeography of Rusa unicolor and R. timorensis. Ecology and Evolution, 8(3), 1465–1479. https://doi.org/10.1002/ece3.3754
- Nei, M. 1972. Chapter 9: Genetic Distance Between Populations. Molecular Evolutionary Genetics, 106(949), 283–292. https://doi.org/10.7312/nei-92038-010
- Nei, M. 1987. The Molecular Approach (Vol. 237, Issue 1984).
- Nicholls, T. J., & Minczuk, M. 2014. In D-loop: 40 years of mitochondrial 7S DNA. Experimental Gerontology, 56, 175–181. https://doi.org/10.1016/j.exger.2014.03.027
- Pereira, F., Pereira, L., Van Asch, B., Bradley, D. G., & Amorim, A. 2005. The mtDNA catalogue of all Portuguese autochthonous goat (Capra hircus) breeds: High diversity of female lineages at the western fringe of European distribution. Molecular Ecology, 14(8), 2313–2318. https://doi.org/10.1111/j.1365-294X.2005.02594.x
- Rahayu, S. E. 2010. Keragaman Genetik Pandan Asal Jawa Barat. Makara Sains, 14(2), 158–162.
- Rianti, P., Hutapea, A. L., Rahman, D. A., & Santosa, Y. 2021. Primer design of D-loop region for wild population genetics of Rusa timorensis in Indonesia. IOP Conference Series: Earth and Environmental Science, 948(1), 1–13. https://doi.org/10.1088/1755-1315/948/1/012017
- S. Blair Hedges, Julie Marin, Michael Suleski, M. P., & and Sudhir Kumara. 1925. Title: Tree of life reveals clock-like speciation and diversification S. Blair Hedges. 1–23.
- Suparningtyas, J. F., Dwi Pramudyawardhani, O., Purwoko, D., & Tajuddin, T. 2018. Analisis filogenetik beberapa Klon Karet dengan Marka AFLP. Jurnal Bioteknologi Dan Biosains Indonesia, 5(1), 8–19. http://ejurnal.bppt.go.id/index.php/JBBI
- Taylor, R. W., & Turnbull, D. M. 2005. Mitochondrial DNA mutations in human disease. Nature Reviews Genetics, 6(5), 389–402. https://doi.org/10.1038/nrg1606
- Utami, S. D., Utaminingsih, S., & Sophian, A. 2023. Analisis DNA Hasil Isolasi Pada Produk Pangan Olahan Ikan (Surimi Ikan) Menggunakan Nano Photometer. JRST (Jurnal Riset Sains Dan Teknologi), 7(1), 9–13. https://doi.org/10.30595/jrst.v7i1.15180
- Whitehead, G. K. 1993. Whitehead encyclopedia of deer. 704. https://doi.org/10.3/JQUERY-UI.JS
- Wirdateti. 2012. Keragaman Genetik Rusa Sambar (Rusa unicolor), Pemanfaatan dan Implikasinya Untuk Konservasi. Jurnal Biologi Indonesia, 8(1), 131–139.
- Wu, H. L., & Fang, S. G. 2005. Mitochondrial DNA genetic diversity of black muntjac (Muntiacus crinifrons), an endangered species endemic to China. Biochemical Genetics, 43(7–8), 407–416. https://doi.org/10.1007/s10528-005-6779-x
- Wu, H., Wan, Q. H., & Fang, S. G. 2004. Two genetically distinct units of the Chinese sika deer (cervus nippon): Analyses of mitochondrial DNA variation. Biological Conservation, 119(2), 183–190. https://doi.org/10.1016/j.biocon.2003.10.027
- Y, Y., Widayanti, R., & Artama, T. 2014. Analisis Genetika Molekuler Kuda Sumba Berdasarkan Urutan D-Loop Mitokondria. Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences, 8(1), 23–26. https://doi.org/10.21157/j.ked.hewan.v8i1.1250
- Yuliati, S., Helmi, T. Z., Rinidar, Balqis, U., Erwin, & Rosmaidar. 2019. Kajian Molekuler Karakteristik Gen Calpastatin (Cast) Pada Kambing Boerka (Capra Hircus). Jimvet, 3(4), 206–216. http://www.etd.unsyiah.ac.id/index.php?p=show_detail&id=40853
- Zein, M. S. 2009. Keragaman Daerah Kontrol DNA Mitokondria Rusa Timor (Cervus timorensis timorensis) di Pulau Timor, Alor, dan Pantar. Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati, 12(3), 138–144. https://doi.org/10.24002/biota.v12i3.2799
References
Agisimanto, D., Supriyanto, A. 2007. Keragaman Genetik Pamelo Indonesia Berdasarkan Primer Random Amplified Polymorphic DNA. Jurnal Hortikultura, 17(1), 1–7.
Ando, S., Komiyama, T., Sudo, M., Higaki, Y., Ishida, K., Costello, J. T., & Katayama, K. 2020. The interactive effects of acute exercise and hypoxia on cognitive performance: A narrative review. Scandinavian Journal of Medicine and Science in Sports, 30(3), 384–398. https://doi.org/10.1111/sms.13573
Aulia, S. L., Suwignyo, R. A., & Hasmeda, M. 2021. Optimasi Suhu Annealing untuk Amplifikasi Dna Padi Hasil Persilangan Varietas Tahan Terendam dengan Metode Polymerase Chain Reaction. Sainmatika: Jurnal Ilmiah Matematika Dan Ilmu Pengetahuan Alam, 18(1), 44. https://doi.org/10.31851/sainmatika.v17i3.5805
Avise, J. C., Arnold, J., Ball, R. M., Bermingham, E., Lamb, T., Neigel, J. E., Reeb, C. A., & Saunders, N. C. 1987. INTRASPECIFIC PHYLOGEOGRAPHY: The Mitochondrial DNA Bridge Between Population Genetics and Systematics. Annual Review of Ecology and Systematics, 18(1), 489–522. https://doi.org/10.1146/annurev.es.18.110187.002421
Balakrishnan, C. N., Monfort, S. L., Gaur, A., Singh, L., & Sorenson, M. D. 2003. Phylogeography and conservation genetics of Eld’s deer (Cervus eldi). Molecular Ecology, 12(1), 1–10. https://doi.org/10.1046/j.1365-294X.2003.01751.x
Dauzery, E., & Randi, E. 1997. The mitochondrial control region of Cervidae: Evolutionary patterns and phylogenetic content. Molecular Biology and Evolution, 14(11), 1154–1166. https://doi.org/10.1093/oxfordjournals.molbev.a025725
Ghazi, M. G., Hussain, S. A., & Gupta, S. K. 2021. Detection of 40 bp tandem repeat motif and associated insertions and deletions (INDEL) in the mitochondrial DNA control region of Sambar deer (Rusa unicolor). Molecular Biology Reports, 48(5), 4129–4135. https://doi.org/10.1007/s11033-021-06426-6
Hasibuan, F. E. B., Mantiri, F. R., & Rumende, R. R. . 2017. KAJIAN VARIASI SEKUNES INTRASPESIES DAN FILOGENETIK MONYET HITAM SULAWESI (Macaca nigra) DENGAN MENGGUNAKAN GEN COI. Jurnal Ilmiah Sains, 17(1), 59–67. https://doi.org/10.35799/jis.17.1.2017.15558
Hisheh, S., Westerman, M., & Schmitt, L. H. 1998. Biogeography of the Indonesian archipelago: Mitochondrial DNA variation in the fruit bat, Eonycteris spelaea. Biological Journal of the Linnean Society, 65(3), 329–345. https://doi.org/10.1006/bijl.1998.0244
Hobbs, J. P. A., Frisch, A. J., Ford, B. M., Thums, M., Saenz-Agudelo, P., Furby, K. A., & Berumen, M. L. 2013. Taxonomic, Spatial and Temporal Patterns of Bleaching in Anemones Inhabited by Anemonefishes. PLoS ONE, 8(8), 1–12. https://doi.org/10.1371/journal.pone.0070966
Hu, J., Fang, S. G., & Wan, Q. H. 2006. Genetic diversity of Chinese water deer (Hydropotes inermis inermis): Implications for conservation. Biochemical Genetics, 44(3–4), 161–172. https://doi.org/10.1007/s10528-006-9020-7
Liu, B., Tao, W., Feng, D., Wang, Y., Heizatuola, N., Ahemetbai, T., & Wu, W. 2022. Revealing Genetic Diversity and Population Structure of Endangered Altay White-Headed Cattle Population Using 100 k SNP Markers. Animals, 12(22), 1–17. https://doi.org/10.3390/ani12223214
Liu, R. Y., Lei, C. Z., Liu, S. H., & Yang, G. S. 2007. Genetic diversity and origin of Chinese domestic goats revealed by complete mtDNA D-loop sequence variation. Asian-Australasian Journal of Animal Sciences, 20(2), 178–183. https://doi.org/10.5713/ajas.2007.178
Liu, R. Y., Yang, G. S., & Lei, C. Z. 2006. The genetic diversity of mtDNA D-loop and the origin of Chinese goats. Acta Genetica Sinica, 33(5), 420–428. https://doi.org/10.1016/S0379-4172(06)60069-3
Martins, R. F., Schmidt, A., Lenz, D., Wilting, A., & Fickel, J. 2018. Human-mediated introduction of introgressed deer across Wallace’s line: Historical biogeography of Rusa unicolor and R. timorensis. Ecology and Evolution, 8(3), 1465–1479. https://doi.org/10.1002/ece3.3754
Nei, M. 1972. Chapter 9: Genetic Distance Between Populations. Molecular Evolutionary Genetics, 106(949), 283–292. https://doi.org/10.7312/nei-92038-010
Nei, M. 1987. The Molecular Approach (Vol. 237, Issue 1984).
Nicholls, T. J., & Minczuk, M. 2014. In D-loop: 40 years of mitochondrial 7S DNA. Experimental Gerontology, 56, 175–181. https://doi.org/10.1016/j.exger.2014.03.027
Pereira, F., Pereira, L., Van Asch, B., Bradley, D. G., & Amorim, A. 2005. The mtDNA catalogue of all Portuguese autochthonous goat (Capra hircus) breeds: High diversity of female lineages at the western fringe of European distribution. Molecular Ecology, 14(8), 2313–2318. https://doi.org/10.1111/j.1365-294X.2005.02594.x
Rahayu, S. E. 2010. Keragaman Genetik Pandan Asal Jawa Barat. Makara Sains, 14(2), 158–162.
Rianti, P., Hutapea, A. L., Rahman, D. A., & Santosa, Y. 2021. Primer design of D-loop region for wild population genetics of Rusa timorensis in Indonesia. IOP Conference Series: Earth and Environmental Science, 948(1), 1–13. https://doi.org/10.1088/1755-1315/948/1/012017
S. Blair Hedges, Julie Marin, Michael Suleski, M. P., & and Sudhir Kumara. 1925. Title: Tree of life reveals clock-like speciation and diversification S. Blair Hedges. 1–23.
Suparningtyas, J. F., Dwi Pramudyawardhani, O., Purwoko, D., & Tajuddin, T. 2018. Analisis filogenetik beberapa Klon Karet dengan Marka AFLP. Jurnal Bioteknologi Dan Biosains Indonesia, 5(1), 8–19. http://ejurnal.bppt.go.id/index.php/JBBI
Taylor, R. W., & Turnbull, D. M. 2005. Mitochondrial DNA mutations in human disease. Nature Reviews Genetics, 6(5), 389–402. https://doi.org/10.1038/nrg1606
Utami, S. D., Utaminingsih, S., & Sophian, A. 2023. Analisis DNA Hasil Isolasi Pada Produk Pangan Olahan Ikan (Surimi Ikan) Menggunakan Nano Photometer. JRST (Jurnal Riset Sains Dan Teknologi), 7(1), 9–13. https://doi.org/10.30595/jrst.v7i1.15180
Whitehead, G. K. 1993. Whitehead encyclopedia of deer. 704. https://doi.org/10.3/JQUERY-UI.JS
Wirdateti. 2012. Keragaman Genetik Rusa Sambar (Rusa unicolor), Pemanfaatan dan Implikasinya Untuk Konservasi. Jurnal Biologi Indonesia, 8(1), 131–139.
Wu, H. L., & Fang, S. G. 2005. Mitochondrial DNA genetic diversity of black muntjac (Muntiacus crinifrons), an endangered species endemic to China. Biochemical Genetics, 43(7–8), 407–416. https://doi.org/10.1007/s10528-005-6779-x
Wu, H., Wan, Q. H., & Fang, S. G. 2004. Two genetically distinct units of the Chinese sika deer (cervus nippon): Analyses of mitochondrial DNA variation. Biological Conservation, 119(2), 183–190. https://doi.org/10.1016/j.biocon.2003.10.027
Y, Y., Widayanti, R., & Artama, T. 2014. Analisis Genetika Molekuler Kuda Sumba Berdasarkan Urutan D-Loop Mitokondria. Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences, 8(1), 23–26. https://doi.org/10.21157/j.ked.hewan.v8i1.1250
Yuliati, S., Helmi, T. Z., Rinidar, Balqis, U., Erwin, & Rosmaidar. 2019. Kajian Molekuler Karakteristik Gen Calpastatin (Cast) Pada Kambing Boerka (Capra Hircus). Jimvet, 3(4), 206–216. http://www.etd.unsyiah.ac.id/index.php?p=show_detail&id=40853
Zein, M. S. 2009. Keragaman Daerah Kontrol DNA Mitokondria Rusa Timor (Cervus timorensis timorensis) di Pulau Timor, Alor, dan Pantar. Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati, 12(3), 138–144. https://doi.org/10.24002/biota.v12i3.2799