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Abstract
The Sambar deer (Cervus unicolor) is a species facing the threat of population decline due to poaching. This study aims to analyze the genetic diversity of Sambar Deer from East Kalimantan using the Inter Simple Sequence Repeat (ISSR) molecular marker with primers P01 and P02. There were 10 sambar deer, eight females and two males, from Pembibitan Ternak dan Hijuan Pakan Ternak, Penajam Paser Utara, East Kalimantan. Blood samples were obtained from the jugular vein using an EDTA project tube. The samples were processed through Polymerase Chain Reaction (PCR) and electrophoresis to detect polymorphism patterns. This research showed a DNA average purity of 1.382 and an average concentration of 13.205; primer P01 produced three haplotype diversity, and primer P02 produced two haplotype diversity. The Polymorphic Information Content (PIC) values were 0.632 for primer P01 and 0.304 for primer P02. Dendrogram analysis revealed three population clusters based on genetic diversity. The conclusion indicates polymorphism, with primer P01 showing higher polymorphism than primer P02.
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References
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References
Askari, N., Abadi, M. M., & Baghizadeh, A. 2011. ISSR markers for assessing DNA polymorphism and genetic characterization of cattle , goat and sheep popula- tions. 9(3), 222–229.
Destomo, A., Putra, W., Hartati, Handiwirawan, E., Mariyono, Elieser, S., Karim, N., Ramadhan, M., Dakhlan, A., Kurniawati, D., & Hasbi. 2024. Association of Two ISSR Markers with the Growth Traits of Saburai Does Short Communication Association of Two ISSR Markers with the Growth Traits of Saburai Does ( Capra hircus ). October. https://doi.org/10.9775/kvfd.2024.32730
Etta, H. 2023. Finger Printing of Three Indigenous Goat (Capra aegagrus Hircus) Breeds in Nigeria Using ISSR Marker. In Science (pp. 0–11). Science.
Febrita, R. E., & Amaniyah, M. 2022. Penentuan Kekerabatan Hewan Berdasarkan Struktur Protein IGF2 Menggunakan Metode K-Means dan N-Gram. 9(2).
Kol, N. V, Evsyukov, A. N., Ruzina, M. N., Shimiit, L. V, & Sulimova, G. E. 2010. Analysis of the Genetic Structure of Tuvinian Short Fat Tailed Sheep Populations with the Use of the ISSR PCR Method. 46(12), 1462–1470. https://doi.org/10.1134/S1022795410120094
Kol, N. V, & Lazebny, O. E. 2006. Polymorphism of ISSR – PCR Markers in Tuvinian Population of Reindeer Rangifer tarandus L . 42(12), 1464–1466. https://doi.org/10.1134/S1022795406120167
Kostova, M., & Bojinov, B. 2018. Application of ISSR markers for detection of genetic variation in two Bulgarian autochthonous goat breeds. 24(6), 1109–1113.
Puspa Pratiwi, A. 2014. Variasi Genetik Attacus atlas L. (Lepidoptera: Saturniidae) Berdasar Penanda Molekuler ISSR (Inter Simple Sequence Repeat). Biogenesis: Jurnal Ilmiah Biologi, 2(1), 21–29. https://doi.org/10.24252/bio.v2i1.464
Putra, W., Margawati, E., Furqon, A., Puja, I., & Hasbi, H. 2024. GENETIC VARIATION IN TWO PHENOTYPES OF BALI CATTLE (Bos javanicus) INFERRED BY (AG)9C ISSR MARKER. 27(092), 1–6.
Sutoyo. 2010. Keanekaragaman Hayati Indonesia Suatu Tinjauan : Masalah dan Pemecahannya. 10, 101–106.
Wardana, A., & Muslih, M. 2021. Comparison the Quality of Template DNA isolated by Column Method with and without Centrifugation.
Wirdateti. 2012. Keragaman genetik Rusa Sambar (Rusa unicolor), pemanfaatan dan implikasinya untuk konservasi. Jurnal Biologi Indonesia, 8(1), 131–139.