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Abstract

This study aimed to detect single nucleotide polymorphisms (SNPs) in intron-2 on growth hormone receptor (GHR) gene in Papua local chickens using the PCR-RFLP method to study its relationship with growth characteristics. Data on the bodyweight of 49 chickens aged 1, 2, 3, and 4 months (22 males, 27 females) and DNA samples were used for this study. The DNA fragment of size 718 bp in intron-2 of the GHR gene from the study chicken was successfully amplified using a pair of specific primers. The PCR-RFLP/HindIII analysis results found this locus's two genotypes (HindIII++ and HindIII--). HindIII+ and HindIII- alleles were 0.02 and 0.98, respectively.

Keywords

polymorphism GHR gene PCR-RFLP HindIII Papua local chickens.

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How to Cite
Mu’in, M. A., & Murwanto, A. G. (2021). GHR/HindIII Locus Polymorphisms in Intron-2 GHR Gene of Papua Local Chicken. Jurnal Sain Peternakan Indonesia, 16(4), 315–321. https://doi.org/10.31186/jspi.id.16.4.315-321
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References

  1. Artama, W.T. 1991. Rekayasa Genetika. Pusat Antar Universitas – Bioteknologi, Universitas Gadjah Mada, Yogyakarta.
  2. Bingxue, Y., D. Xuemei, F. Jing, H. Xiaoxiang, W. Changxin and L. Ning, 2003. Single nucleotide polymorphism analysis in chicken growth hormone gene and its associations with growth and carcass traits. Chinese Science Bulletin, 48(15): 1561 – 1564.
  3. Burnside, J., S.S. Liou, and L.A. Cogburn, 1991. Molecular cloning of the chicken growth hormone receptor complementary deoxyribonucleic acid: Mutation of the gene in sex-linked dwarf chickens. Endocrinology 128 (6): 3183-3192.
  4. Enayati, B. and G. Rahimi-Mianji, 2009. Genomic growth hormone, growth hormone receptor and transforming growth factor ?-3 gene polymorphism in breeder hens of Mazandaran native fowls. African J. Biotechnology 8(14): 3154-3159.
  5. Feng, X.P., U. Kuhlein, S.E. Aggrey, J.S. Gavora, D. Zadworny, 1997. Trait association of genetic markers in the growth hormone and growth hormone receptor gene in White Leghorn strain. Poult. Sci. 76: 1770 – 1775.
  6. Feng, X.P., U. Kuhnlein, R.W. Fairfull, S.E. Aggrey, J. Yao, and D. Zadworny, 1998. A genetic markers in growth hormone receptor gene associated with body weight in chickens. J. Hered. 89: 355 – 358.
  7. Harris, H., 1994. Dasar-Dasar Genetika Biokemis Manusia. Edisi ketiga, diperbaharui Penerjemah: dr. Abdul Salam M. Sofro, Ph.D. Gadjah Mada University Press, Yogyakarta.
  8. Haryani, E., 1999. Keragaman Morfologi dan Morfogenetik Ayam Kampung di Kabupaten Manokwari. Skripsi. Fakultas Pertanian, Universitas Cenderawasih, Manokwari.
  9. Kirby, L.T., 1990. DNA Fingerprinting: An Introduction. M. Stockton Press. New York.
  10. Lawrence, T.J.J. and V.R. Fowler, 2002. Growth of Farm Animals. 2nd ed. CABI Publishing, New York.
  11. Li, H.F., W. Zhu, K. Chen, X. Wu, Q. Tang, and Y. Gao, 2008. Associations between GHR and IGF-1 gene polymorphism and reproductive traits in Wenchang chickens. Turk. J. Vet. Anim. Sci. 32 (4): 281-285.
  12. Lumatauw, S. A.L. Killian, dan A. Supriyantono, 1995. Identifikasi Sifat-sifat Morfogenetik Ayam Buras di Irian Jaya. Laporan penelitian. Fakultas Pertanian, Universitas Cenderawasih, Manokwari.
  13. Mu’in, M.A., 2000. Analisis potensi genetik beberapa ukuran tubuh ayam kampung. Jurnal Irian Jaya Agro, 7(2): 38- 42.
  14. Muladno, 2002. Seputar Teknologi Rekayasa Genetika. Pustaka Wirausaha Muda dan USESE Foundation, Bogor.
  15. Nei, M. and D. Kumar, 2000. Molecuar Evolution and Phylogenetics. Oxford University Press, New York.
  16. Nie, Q., B. Sun, D. Zhang, C. Luo, N.A. Ishag, M. Lei, G. Yang, and X. Zhang, 2005. High diversity of the chicken growth hormone gene and effect on growth and carcass traits. J. Hered. 96(6):698-703.
  17. Rahayu, B.W.I. (1990). Pengkajian Beberapa Sifat Phenotip Ayam Kampung di Kota Manokwari. Skripsi. Fakultas Pertanian, Universitas Cenderawasih, Manokwari.
  18. Roy, B.G., V.K. Saxena, U. Roy, and M.C. Kataria, 2020. PCR-RFLP study of candidate genes for egg production in layer chicken. Arch Anim Poult Sci, 1(3): 52-59.
  19. Sambrook, J., Fritcsh and T. Maniatis, 1989. Molecular Cloning Laboratory Manual. Second Edition, Vol. 3. Cold Spring Harbor Laboratory Press. USA.
  20. Smith, R.G. and E.W. Brascamp, 1990. Genetic impact by advanced breeding technologies and gene transfer. In: Biotechnology for Control of Growth and Product Quality in Meat Production: Implications and Acceptability. Proceedings of an International Symposium, Washington, D.C. (5-7 Dec. 1990).
  21. Steel, R.G.D. and J.H. Torrie, 1980. Principles and Procedures of Statistics. McGraw_Hill Publishing Co., New York.
  22. Stephen, C.Y. Ip., X. Zhang, and C.L. Frederick, 2001. Genomic growth hormone gene polymorphism in native chinese chickens. Experimental Biology and Medicine 226: 458-462.