Main Article Content

Abstract

This research has been carried out for a month using eighty three-month-old female Cihateup ducks and has been used to study the influence of glutathione on the endogen antioxidant response and  histological illeum. The separation of glutathione was carried out using the distillation technique. Each experimental group consisted of 20 ducks, each treated with G0 = without glutathione; G1= Administration of glutathione 150 µL/head; G2 = Administration of glutathione 175 µL/head; G3= Administration of glutathione 175 µL/head. Tissue and blood sampling was collected at the end of the study by EDTA tube and a fixative solution, and tissue preparations were made using the Mallory-Asan technique and analyzed using a binocular microscope. Antioxidant levels were measured using a spectrophotometer technique based on the instructions of the Randox Kit. The results showed that glutathione affected (P<0.05) morphometric ileum (villi, Peyeri's plaque and goblet cells) and increased with increasing glutathione level and endogenous antioxidant response. It was concluded that glutathione is able to stimulate protein and lipid anabolism, as well as hormonal signals related to ileal tissue growth and antioxidants.

Keywords

additive physiologic duck

Article Details

How to Cite
Mushawwir, A., Suwarno, N., & Aritonang, H. (2022). Impact of Glutathione Administration on Antioxidant Levels and Ileum Histologic of Growth Phase of Cihateup Duck in Extensively Maintained. Jurnal Sain Peternakan Indonesia, 17(3), 142–148. https://doi.org/10.31186/jspi.id.17.3.142-148

References

  1. Adriani L, Abun and A. Mushawwir. 2015. Effect of dietary supplementation of Jengkol (Pithecellobium jiringa) skin extract on blood biochemistry and gut flora of broiler chicken. International Journal of Poultry Science 14(3): 407-410. DOI: 10.3923/ijps.2015.407.410
  2. Adriani L. and A. Mushawwir. 2020. Correlation between blood parameters, physiological and liver gene expression levels in native laying hens under heat stress. IOP Conf. Series: Earth and Environmental Science 466 (7): 1-7. DOI:10.1088/1755-1315/788/1/012091
  3. Adriani L., A. Mushawwir, C. Kumalasari, L. Nurlaeni, R. Lesmana and U. Rosani. 2021. “Improving Blood Protein and Albumin Level Using Dried Probiotic Yogurt in Broiler Chicken”, Jordan Journal of Biological Sciences, 14(5): 1021-1024. DOI:10.54319/jjbs/140521
  4. Adriani, L., A. Mushawwir, B.R. Anastasia and B. Rahayu. 2018. Effect of combination chitosan and turmeric powder (curcuma domestica val.) For improving blood lipid profile in broilers. Scientific Papers. Series D. Animal Science. LXI (1):225-229. DOI: 10.3244/j.ass.2018.99.021
  5. Allen J.D., L. W. Hall, R.J. Collier and J.F. Smith. 2015. Effect of core body temperature, time of day, and climate conditions on behavioral patterns of lactating dairy cows experiencing mild to moderate heat stress. Journal Dairy Science. 98(3): 118-27. DOI: 10.3168/jds.2013-7704
  6. Ammer S, C. Lambertz, D. von Soosten, K. Zimmer, U. Meyer, S. Dänicke and M. Gauly. 2018. Impact of diet composition and temperature-humidity index on water and dry matter intake of high-yielding dairy cows. Journal Animal Physiology and Animal Nutrition. 102 (3): 103-113. DOI: 10.1111/jpn.12664
  7. Carrol E.C., L. Jin, A. Mori, M.N. Wolf, E. Oleszycka, H.B.T. Moran, S. Mansouri, C.P. McEntee, E. Lambe, E.M. Agger, P. Andersen, C. Cunningham, P. Hertzog, K.A. Fitzgerald, A.G. Bowie and E.C. Lavelle. 2016. The Vaccine Adjuvant Chitosan Promotes Celluler Immunity Via DNA Sensor Cgas-STING-Dependent Induction of Type I Interferons. Immunity. 44(1): 1-12. DOI: 10.1016/j.immuni.2016.02.004
  8. Dinana, A., D. Latipudin, D. Darwis dan A. Mushawwir. 2019. Profil enzim transaminase ayam ras petelur yang diberi kitosan iradiasi. Jurnal Nutrisi Ternak Tropis dan Ilmu Pakan. 1 (1):6-15. DOI: http://dx.doi.org/10.24198/jnttip. v1i1.25425
  9. Fabris T.F., J. Laporta and F.N. Corra. 2017. Effect of nutritional immunomodulation and heat stress during the dry period on subsequent performance of cows. Journal Dairy Science 100: 6733-6742. DOI: https://DOI.org/10.3168/jds.2016-12313
  10. Gehrke N., C. Mertens, T. Zillinger, J. Wenzel, T. Bald, S. Zahn, T. Tuting, G.W. Hartmann and W. Barchet. 2013. Oxidative Damage of DNA Confers Resistance to Cytosolic Nuclease TREX1 Degradation and Pontentiates STING-Dependent Immune Sensing. Immunity 39:482-495. DOI: 0.1016/j.immuni.2013.08.004
  11. Gray L.R., M.R. Sultana and A. J. Rauckhorst. 2015. Hepatic mitochondrial pyruvate carrier 1 is required for efficient regulation of gluconeogenesis and whole-body glucose homeostatis. Cell Metabolism. 22: 669-681. DOI: https://DOI.org/10.1016/ j.cmet.2015.07.027
  12. Hernawan E, L. Adriani and A. Mushawwir, C. Cahyani and D. Darwis. 2017. Effect of dietary supplementation of chitosan on blood biochemical profile of laying hens. Pakistan Journal Nutrition 16: 696-699. DOI: https://dx.DOI.org/10.3923/pjn.2017.696.699
  13. Ippolito D. L., J. A. Lewis, C. Yu, L.R. Leon and J.D. Stallings. 2014. Alteration in circulating metabolites during and after heat stress in the conscious rat: potential biomarkers of exposure and organ specific injury. BMC Physiology 14: 23-29.DOI: https://dx.DOI.org/ 10.1186%2Fs12899-014-0014-0
  14. István F., L. Zsolt and O. László. 2020. Relationship of dairy heifer reproduction with survival to first calving, milk yield and culling risk in the first lactation. Asian-Australasian Journal of Animal Sciences. 33: 1360-1368. DOI: 10.5713/ajas.19.0474
  15. Jiwandini A., H. Burhanudin dan A. Mushawwir. 2020. Kadar enzim transaminase (sgpt, sgot) dan gamma glutamyl transpeptidase (γ-gt) pada ayam petelur fase layer yang diberi ekstrak pegagan (Centella asiatica). Jurnal Nutrisi Ternak Tropis dan Ilmu Pakan. 2(2):112-119. DOI: https://doi.org/10.24198/jnttip.v2i2.27389
  16. Kamil K.A., D. Latipudin, A. Mushawwir, D. Rahmat and R. L. Balia. 2020. The Effects of ginger volatile oil (GVO) on the metabolic profile of glycolytic pathway, free radical and antioxidant activities of heat-stressed cihateup duck. International Journal on Advanced Science, Engineering and Information Technology 10:1228-1233. DOI: 10.1817/ijaseit.10.3.11117
  17. Khan S., K. Anwar, K. Kaleem, A. Saeed, H. Nabi, A. Hayat, Z. Ahmad, F. Hayan and Safirullah. 2015. Study of phenotypic and morphometric characteristics of Achai cattle at Livestock Research and Development Station Dir (Lower), Pakistan. Pakistan Journal Nutrition 14: 201-203. DOI: 10.4737/pjn.2015.452.774
  18. Kharazi A.Y., D. Latipudin, N. Suwarno, T. Puspitasari, N. Nuryanthi and A. Mushawwir. 2022. Lipogenesis in Sentul chickens of starter phase inhibited by irradiated chitosan. IAP Conference Proceedings 1001: 1-7. doi:10.1088/1755-1315/1001/1/012021
  19. Loyau T, S. Metayer-Coustard, C. Berri, S. Crochet, S. Cailleau-Audouin, M. Sannier, P. Chartrin, C. Praud, C. Hennequet-Antier, N. Rideau, N. Courousse, N. Mignon-Grasteau, N. Everaert, M. J. Duclos, S. Yahav, S. Tesseraud and A. Collin. 2014. Thermal manipulation during embryogenesis has longterm effects on muscle and liver metabolism in fast-growing chickens. PLoS One 9: e105339. http://dx.DOI.org/ plosone/10.1016/P.2014.08.444
  20. Mushawwir A, J. Arifin, D. Darwis, T. Puspitasari, D.S. Pengerteni, N. Nuryanthi and R. Permana. 2020e. Liver metabolic activities of Pasundan cattle induced by irradiated chitosan. Biodiversitas. 21: 5571-5578. https://DOI.org/10.13057/ biodiv/d211202
  21. Mushawwir A, R. Permana, D. Darwin, T. Puspitasari, D. S. Pangerteni, N. Nuryanthi and N. Suwarno. 2021c. Enhancement of the liver histologic of broiler induced by irradiated chitosan (IC). IAP Conference Proceedings 2381: 0200461-0200467. DOI: https://DOI.org/10.1063/5.0066271
  22. Mushawwir A., K.Y. Yong, L. Adriani, E. Hernawan and K.A. Kamil. 2010. The Fluctuation Effect of Atmospheric Ammonia (NH3) Exposure and Microclimate on Hereford Bulls Hematochemical. Journal of The Indonesian Tropical Animal Agriculture 35: 232-238. DOI: https://DOI.org/10.14710/jitaa.35.4.232-238
  23. Mushawwir A., L. Adriani and K.A. Kamil. 2011. Prediction models for olfactory metabolic and sows % RNAreticulocyt (RNArt) by measurement of atmospheric ammonia exposure and microclimate level. Journal of The Indonesian Tropical Animal Agriculture. 36: 14-20. DOI: https://DOI.org/10.14710/jitaa.36.1.14-20
  24. Mushawwir A., U.H. Tanuwiria, K.A. Kamil, L. Adriani, R. Wiradimadja and N. Suwarno. 2018. Evaluation of haematological responses and blood biochemical parameters of heat-stressed broilers with dietary supplementation of javanese ginger powder (Curcuma xanthorrhiza) and garlic extract (Allium sativum). International Journal of Poultry Science.17: 452-458. DOI: 10.3923/ijps.2018.452.458
  25. Mushawwir, A., N. Suwarno dan R. Permana. 2020a. Profil Total Lemak dan Protein Hati Puyuh Fase Grower dan Layer. Jurnal Ilmu dan Industri Peternakan. 6(2):65-76. DOI: http://dx.doi.org/10.24252/jiip.v6i2.18312
  26. Mushawwir, A., A.A. Yulianti dan N. Suwarno. 2020b. Histologi Liver Burung Puyuh dengan Pemberian Minyak Atsiri Bawang Putih. Jurnal Ilmu dan Teknologi Peternakan. 8(1):1-7. DOI: http://dx.doi.org/10.24252/jitp.v8i1.32758
  27. Mushawwir, A., N. Suwarno dan D. Latipudin. 2020c. Profil metabolik jalur glikogenolisis puyuh dalam kondisi stres panas dengan pemberian diallyl n-sulfida¬ (Dn-S) organic. Jurnal Galung Tropika. 9:48-59. DOI: http://dx.doi.org/10.31850/jgt.v9i1.519
  28. Mushawwir, A., N. Suwarno dan R. Permana. 2020d. Profil non-esterified fatty acids (NEFA) dan trigliserida ayam sentul pada sistem pemeliharaan berbeda. Jurnal Ilmu dan Industri Peternakan. 6:14-24. DOI: 10.24252/jiip.v6i1.14445
  29. Mushawwir A., R. Permana, D. Latipudin and N. Suwarno. 2021a. Organic Diallyl-n-Sulfide (Dn-S) inhibited the glycogenolysis pathway and heart failure of heat-stressed laying hens. IOP Conf. Series: Earth and Environmental Science. 788: 1-7. DOI:10.1088/1755-1315/788/1/012091
  30. Mushawwir, A., N. Suwarno dan R. Permana. 2021b. Dialil n-Sulfida Organik Menurunkan Kadar Lipid Plasma Darah dan Hati Itik Cihateup Fase Grower. Jurnal Ilmu dan Teknologi Peternakan Tropis. 8(1):19-25. DOI: 10.33772/jitro.v8i1.15128
  31. Mushawwir, A., R. Permana, D. Darwin, T. Puspitasari, D.S. Pangerteni, N. Nuryanthi and N. Suwarno. 2021c. Enhancement of the liver histologic of broiler induced by irradiated chitosan (IC). IAP Conference Proceedings 2381: 0200461-0200467. DOI: 10.1063/5.0066271
  32. Mushawwir, A., D. Latipudin, R. Permana and N. Suwarno. 2021d. diallyl-n-sulfide of garlic inhibits glycogenolysis in heat-stressed laying Sentul Chicken. Jurnal Sain Peternakan Indonesia 16 (4), 301-307. DOI: 10.31186/jspi.id.16.4.301-307
  33. Na Y.K., H.M. Sang, J.K. Seong, K.K. Eun, O. Mirae, T. Yujiao and Y. J. Se. 2020. Summer season temperature-humidity index threshold for infrared thermography in Hanwoo (Bos taurus coreanae) heifers. Asian-Australasian Journal of Animal Sciences. 33: 1691-1698. DOI: 10.3791/52703
  34. Nurmalia, V.R., D. Rusmana dan A. Mushawwir. 2020. Kadar Glukosa Dan Trigliserida Ayam Ras Petelur Fase Layer Yang Diberi Ransum Mengandung Ekstrak Pegagan (Centella asiatica). Jurnl Ilmu Nutrisi Ternak Tropis dan Ilmu Pakan. 2(4):217-224. DOI: 10.24198/jnttip. v2i4.27396.
  35. Rahmania H., R. Permana, D. Latipudin, N. Suwarno, T. Puspitasari, N. Nuryanthi and A. Mushawwir. 2022. Enhancement of the liver status of Sentul chickens from the starter phase induced by irradiated chitosan. IAP Conference Proceedings 1001: 1-7. doi:10.1088/1755-1315/1001/1/012007
  36. Roland L, M. Drillich, D. Klein-Jobstl and M. Iwernes. 2016. Invited review: Influence of climatic conditions on the development, performance, and health of claves. Journal of Dairy Science. 99: 2438-2452. DOI: 10.3168/jds.2015-9901
  37. Sang-Ho M, K. Eun-Kyung, S.J. Se, T. Yujiao, S. Hye-Jin, S. Y. Yeong, C. Sanguk and O. Mirae. 2018. Fatty acid compositions, free radical scavenging activities, and antioxidative enzyme activities of high-preference and low-preference beef cuts of Hanwoo (Bos taurus coreanae) cows. 31: 1974-1979. DOI: 11.1017/S17517311150008963
  38. Siregar R.H., D. Latipudin dan A. Mushawwir. 2020. Profil lipid darah ayam ras petelur yang di beri kitosan iradiasi. Jurnal Nutrisi Ternak Tropis dan Ilmu Pakan. 2(1):1-8. DOI : 10.24198/jnttip.v2i1.25707
  39. Suwarno, N. dan A. Mushawwir. 2019. Model Prediksi Metabolit Melalui Jalur Glikogenolisis Berdasarkan Fluktuasi Mikroklimat Lingkungan Kandang Sapi Perah. Jurnal Ilmu dan Industri Peternakan. 5 (2):77-86. DOI: http://dx.doi.org/10.24252/jiip.v5i2.11884
  40. Tanuwiria U.H. and A. Mushawwir. 2020a. Hematological and antioxidants responses of dairy cow fed with a combination of feed and duckweed (Lemna minor) as a mixture for improving milk biosynthesis. Biodiversitas. 21:4741-4746. DOI https://DOI.org/10.13057/biodiv/ d211038
  41. Tanuwiria U.H., I. Susilawati, D. S. Tasrifin, L. B. Salman and A. Mushawwir. 2022. Behavioral, physiological, and blood biochemistry of Friesian Holstein dairy cattle at different altitudes in West Java, Indonesia. Biodiversitas. 23(1): 533-539. DOI: 10.13057/biodiv/d230157
  42. Tanuwiria U.H., D. Tasrifin dan A. Mushawwir. 2020b. Respon gamma glutamil transpeptidase (-gt) dan kadar glukosa sapi perah pada ketinggian tempat (altitude) yang berbeda. Jurnal Ilmu dan Industri Peternakan. 6:25-34. DOI: 10.24252/jiip. v6i1.14446
  43. Tian H, W. Wang, and N. Zheng. 2015. Identification of diagnostic biomarkers and metabolic pathway shifts of heat-stressed lactating dairy cows. Journal of proteomics. 125(12): 17-28. DOI: 10.1016/j.jprot.2015.04.014
  44. Xu B., M. Chen and X. Ji. 2015. Metabolomic profiles reveal key metabolic changes in heat stress-treated mouse Sertoli cells. Toxicology In Vitro. 29(3): 1745-1752. DOI: 10.1016/j.tiv.2015.07.009