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Abstract

This study aims to obtain MSTN gene diversity and MSTN gene association. The materials used in this study were 96 KUB chickens and 96 KUB chicken blood samples consisting of 41 males and 55 females. This research method is an experiment. Data collected included body weight, weight gain and body size of male and female KUB chickens, and diversity and association of MSTN genes. Data analysis used mean difference test (T-test), T2-Hotelling, Principal Component Analysis (PCA), Genotype and allele frequencies, Hardy-Weinberg equilibrium, Heterozygosity and Polymorphic Information Content (PIC). The results showed that body weight at 1 and 2 months of age, body weight gain at 1-2 months and body size of male KUB chickens at two months were significantly (P<0.05) higher than female KUB chickens. The body size characteristic of male and female KUB chickens is chest circumference (CC) while the body shape characteristic is wing length (WL). The MSTN|MspI gene in KUB chickens is polymorphic. The KUB chicken population is in Hardy-Weinberg imbalance. Heterozygosity of the KUB chicken population showed a value of Ho (0.31) < He (0.51). The MSTN| MspI gene of KUB chicken was associated with body weight, weight gain and body measurements and the best genotype was (-/-).

Keywords

Diversity KUB chicken Myostatin gene (MSTN) Msp1 enzyme

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How to Cite
Sugara, M. F., Depison, D., & Gushairiyanto, G. (2023). Characterization of Myostatin (MSTN) Gene Using PCR-RFLP Method in KUB Chicken. Jurnal Sain Peternakan Indonesia, 18(3), 169–176. https://doi.org/10.31186/jspi.id.18.3.169-176
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References

  1. Aiello, D., K, Patel, E, Lasagna. 2018. The myostatin gene: an overview of mechanisms of action and its relevance to livestock animals. Animal Genetics. 49(1): 505-519.
  2. Allendorf, F. W, G. Luikart, S. N. Aitken 2013. Conservation and the Genetics of Populations. Blackwell Publishing, Oxford, UK.
  3. Al-Sobri, S. N., Depison, E. Wiyanto. 2022. Myostatin (MSTN) gene polymorphism using PCR-RFLP method in Kerinci Ducks. Journal of Indonesian Animal Science 17(4): 229-237.
  4. Bhattacharya, T.K., R. Shukla, R.N. Chatterjee and S. K. Bhanja. 2019. Comparative analysis of gene by RNA interference increased body weight in chicken. J Biotechnol. 241(2): 61-68.
  5. Botstein D., R.L. White, M. Skolnick, and R.W. Davis. 1980. Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am. J. Hum. Genet. 32 (3): 314-331.
  6. Depison, Gushairiyanto and D. Irmaya, 2022. Characterization phenotype and genetic distance some of the native chicken strains in Jambi Province Indonesia. Iraqi Journal of Agricultural Sciences 53(5):1154- 1166.
  7. Fastawa, R., C. Sumantri, A. Gunawan and S. Nurtini. 2019. Feeding anti-myostatin to parents and myostatin gene diversity and its association with carcass cuts of Sentul (F1) chicks. Journal of Production Science and Technology of Animal Products 07(1): 22-28.
  8. Ghassani, A. F., Depison, D., & Ediyanto, H. 2022. Association of Quantitative Characteristics with Growth Hormone Gene (GH Gene) in Kerinci Duck Using PCR-RFLP Method. Buletin Peternakan 46 (4): 248-256.
  9. Hapsari, I.P. 2015. Body size and egg production of chickens crossed with local broilers. Bogor. Bogor Agricultural University.
  10. Hernandez, M.R., F.R.L. Buckle, E. Palacios, and B.S. Baron. 2006. Preferential behavior of white shrimp Itopenaeus vannamei (Boone 1931) by progressive temperature-salinity simultaneous interaction. J. Thermal Biology 31: 565-572.
  11. Hidayati, E. Saleh, and T. Aulawi. 2016. Identification of bmpr-1b (bone morphogenetic protein receptor ib) gene diversity in Arabian chickens, Kampung chickens and laying breeds using pcr-rflp. Journal of Animal Husbandry 13(1): 1-12.
  12. Hidayati, E. Saleh, and T. Aulawi. 2016. Identification of bmpr-1b (bone morphogenetic protein receptor ib) gene diversity in Arabian chickens, Kampung chickens and laying breeds using pcr-rflp. Journal of Animal Husbandry 13(1): 1-12.
  13. Hikmawaty, A. Gunawan, R. R. Noor, and Jakaria. 2014. Identification of body size and body shape of Balinese cattle in several breeding centres through principal component analysis approach. Journal of Animal Products Science and Technology 02(1): 231-237.
  14. Khaerunnisa I, Pramujo M, Arief I I, Budiman C, Gunawan A, Jakaria, andSumantri C. 2016. Polymorphism of the T4842G myostatin gene is associated with carcass characteristics in Indonesian chickens. International Journal Poultry Science15 (8): 316-24.
  15. Lynch, G, S., R, Koopman. 2019. Overcoming nature's paradox in skeletal muscle to optimize animal production. Animal Production Science 59(1): 1957-1969.
  16. Mardiah, T., Depison and Helmi. Phenotypic Diversity and Myostatin (MSTN) Gene of Bangkok Chicken Using PCR-RFLP Method. Livestock Bulletin 45 (4): 233-240.
  17. Nei, M. and S. Kumar. 2000. Molecular evolution and phylogenetics. Oxford University Press. New York.
  18. Nova, T. D., Yurnalis and A.K. Sari. 2016. Genetic diversity of growth hormone gene (gh|mboii) in Sikumbang janti ducks using pcrrflp characterizer. Indonesian Journal of Animal Husbandry 18(1): 44-52.
  19. Prawira, R., Depison, Gushariyanto, and S. Erina. 2021. Relationship of egg morphology with egg weight and DOC weight with body weight of F1 Kampung chicken. Journal of Applied Animal Science 5(1): 19-30.
  20. Putri, A. B. S. R. N., Gushairiyanto and Depison. 2020. Body weight and morphometric characteristics of several local chicken strains. Journal of Tropical Livestock Science and Technology 7(3): 256-263.
  21. Rahayu, F. F., Gushairiyanto and Depison. 2021. Appearance of super native chickens and first-generation (G1) Bangkok chickens until 12 weeks of age. Livestock and Animal Research 19 (3): 326-336.
  22. Ratnawati, S., A. Rubianty, Y. Achadi Y., P.R. Matittaputy. 2020. Development of superior native chickens of the R & D agency after the COVID-19 pandemic in Kupang district, Nusa Tenggara. Faculty of Animal Husbandry, Jendral Soedirman University. ISBN: 978-602-52203-2-6.
  23. Salsabila, S., Depison, and S. Erina. 2022. Morphometric characterization and effect of growth hormone (GH) gene polymorphism on growth traits of Kerinci duck (Anas platyrhynchos). Livestock and Animal Research 20 (3): 300-311.
  24. Sari, M., Depison, Gushariyanto, and E. Wijayanto. 2021. Relationship between egg weight and hatching weight with body weight of Merawang G1 chickens until 4 months of age. Journal of Animal Husbandry 18(2): 147-159.
  25. Sitanggang, E.N., Hasnudi, and Hamdan. 2016. Diversity of qualitative and morphometric traits between ayam kampung, ayam bangkok, ayam katai, ayam birma, ayam bagon and magon in Medan. Journal of Integration Animal Husbandry 3:167-189.
  26. Suhartati, L, Khaerunnisa, A, Gunawan, Rukmiasih, S, Darwati, C, Sumantri, Rizqan. 2020. Short Communication: Identification of the exon 1 myostatin gene polymorphism and its association with slaughtered weight in Indonesian Kampung and Broiler Chicken. Biodiversitas 21 (1): 3893-3897.
  27. Tanjung, A., H.T.S.G. Saragih, Trijoko, H.P. Soenarwan, S. Widianto, L.W.S. Mahardika, B.S. Daryono. 2019. Polymorphism of myostatin gene and its association with body weight traits in a hybrid of gama chicken (Gallus gallus domesticus Linn. 1758). Biodiversitas 20 (11): 3207-3212. https://doi.org/10.13057/biodiv/d201113
  28. Utama M., Depison, Gushairiyanto, H. Ediyanto. 2022. Comparison of Germination, hatchability, and quantitative characteristics of KUB Chickens and Kampung Chickens (G1). Journal of Tropical Animal Science and Technology 9(1): 95-103.
  29. Wang, K., H. Ouyang, Z. Xie, C. Yao, N. Guo, M. Li, H. Jiao, and D. Pang. 2015. Efficient generation of myostatin mutations in pigs using the crispr/cas9 system. Scientific reports 5.1: 16623.DOI:10.1038/srep16623.
  30. Widhianata, H. 2019. Analysis of elders and legitimacy of controlled crosses in oil palm using ssr markers. Agriosta, Journal of Agrotechnology 3(1):26-30.
  31. Zhao, X, H., M. Y. Li, S. S. Xu, J. Sun, G. J. Liu. 2019. Expression of myostatin (mstn) and myogenin (myog) genes in Zi and Rhine Goose and their correlation with carcass traits. Brazilian Journal of Poultry Science 21 (1): 1- 6.